AB233971

Anti-TAGLN/Transgelin antibody [TAGLN/247]

Name: Anti-TAGLN/Transgelin antibody [TAGLN/247] (ab233971) | Abcam
Brand: Abcam Limited
SKU: AB233971
Price: 525 USD
Availability: InStock

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(4 Publications)

Mouse Monoclonal TAGLN/Transgelin antibody. Suitable for IHC-P and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human TAGLN.

View Alternative Names

SM22, WS3-10, TAGLN, Transgelin, 22 kDa actin-binding protein, Protein WS3-10, Smooth muscle protein 22-alpha, SM22-alpha

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TAGLN/Transgelin antibody [TAGLN/247] (AB233971)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TAGLN/Transgelin antibody [TAGLN/247] (AB233971)

Formalin-fixed, paraffin-embedded human colon carcinoma tissue stained for SM22 alpha using ab233971 at 1μg/ml in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TAGLN/Transgelin antibody [TAGLN/247] (AB233971)

Formalin-fixed, paraffin-embedded human colon carcinoma tissue stained for SM22 alpha using ab233971 at 1μg/ml in immunohistochemical analysis.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

TAGLN/247

Isotype

IgG1

Light chain type

kappa

Carrier free

Reacts with

Human

Applications

IHC-P

applications

Immunogen

Recombinant Full Length Protein corresponding to Human TAGLN.

Q01995

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "0.5-1 µg/mL", "IHCP-species-notes": "<p>Incubate with primary antibody for 30 minutes at room temperature.</p> Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A/G

Purification notes

Purified from Bioreactor Concentrate by Protein A/G.

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: PBS, 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TAGLN also known as Transgelin or SM22-alpha is a protein with a molecular weight of approximately 22 kDa. Structurally it belongs to the calponin family and contains an ACT domain which is critical for its actin-binding ability. It is expressed abundantly in smooth muscle cells where it stabilizes cytoskeletal dynamics by interacting with actin filaments. TAGLN also shows expression in fibroblasts and myofibroblasts supporting structural integrity and cellular movement.

Biological function summary

The regulatory involvement of TAGLN in smooth muscle contraction influences cellular activities essential for vascular function. This protein modulates remodeling and differentiation within smooth muscle cells. It can associate with actin and calponin to form complexes that regulate the stability of cytoskeletal structures ensuring effective cellular responses to mechanical stimuli. By linking signal transduction to cytoskeletal mechanics TAGLN facilitates the maintenance of cell shape and tension.

Pathways

TAGLN plays a role in the regulation of the RhoA/Rho kinase pathway contributing to actin cytoskeleton organization and smooth muscle contraction. Additionally it is involved in the WNT signaling pathway influencing cell proliferation and differentiation. In these pathways TAGLN works with proteins like RhoA and beta-catenin to transmit signals from the extracellular environment to the nucleus impacting gene expression and cellular responses.

Disruptions in TAGLN expression associate with vascular diseases and certain cancers. Downregulation or altered expression patterns can lead to smooth muscle cell dysfunction contributing to atherosclerosis. Similarly TAGLN interacts with proteins such as fibronectin and MMP2 in cancer where its altered expression could promote metastasis and tumor progression. Understanding these connections highlights TAGLN's potential as a target for therapeutic interventions in these diseases.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Actin cross-linking/gelling protein (By similarity). Involved in calcium interactions and contractile properties of the cell that may contribute to replicative senescence.

See full target information TAGLN

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

International journal of oncology 63: PubMed37203411

2023

Transgelin promotes ferroptosis to inhibit the malignant progression of esophageal squamous cell carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Qiuyu Chen,Lingyun Zhang,Changshan Wan,Boli Yang,Xiangxu Kong,Xin Xu,Yu Gu,Chen Wang,Xiaotong Liu,Qian Ding,Lanping Zhu,Ying Li,Bangmao Wang,Weilong Zhong

International journal of experimental pathology 103:190-197 PubMed35527237

2022

Expression of airway smooth muscle contractile proteins in children with acute interstitial pneumonia.

Applications

Unspecified application

Species

Unspecified reactive species

Fang Cheng,Tao Lu,Yicheng Wang,Didi Yuan,Zehong Wei,Yongguo Li,Jianbo Li,Renkuan Tang

International journal of molecular sciences 23: PubMed35008490

2021

Circular RNA hsa_circ_0000190 Facilitates the Tumorigenesis and Immune Evasion by Upregulating the Expression of Soluble PD-L1 in Non-Small-Cell Lung Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Yung-Hung Luo,Yi-Ping Yang,Chian-Shiu Chien,Aliaksandr A Yarmishyn,Afeez Adekunle Ishola,Yueh Chien,Yuh-Min Chen,Ping-Hsing Tsai,Tzu-Wei Lin,Mong-Lien Wang,Shih-Hwa Chiou

Frontiers in oncology 11:709486 PubMed34552870

2021

Transgelin Inhibits the Malignant Progression of Esophageal Squamous Cell Carcinomas by Regulating Epithelial-Mesenchymal Transition.

Applications

Unspecified application

Species

Unspecified reactive species

Boli Yang,Qiuyu Chen,Changshan Wan,Siyuan Sun,Lanping Zhu,Zhizhong Zhao,Weilong Zhong,Bangmang Wang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-TAGLN/Transgelin antibody [TAGLN/247]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TAGLN/Transgelin antibody [TAGLN/247] (AB233971)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TAGLN/Transgelin antibody [TAGLN/247] (AB233971)

Key facts

Host species

Clonality

Clone number

Isotype

Light chain type

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

Product promise