Mouse Monoclonal TAGLN/Transgelin antibody. Carrier free. Suitable for Flow Cyt, ICC/IF, IHC-P and reacts with Human samples. Cited in 5 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human TAGLN.
Images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TAGLN/Transgelin antibody [TAGLN/247] - BSA and Azide free (AB212857), expandable thumbnail](https://content.abcam.com/products/images/tagln-transgelin-antibody-tagln-247-bsa-and-azide-free-ab212857--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img9865.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TAGLN/Transgelin antibody [TAGLN/247] - BSA and Azide free (AB212857)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TAGLN/Transgelin antibody [TAGLN/247] - BSA and Azide free (AB212857), expandable thumbnail](https://content.abcam.com/products/images/tagln-transgelin-antibody-tagln-247-bsa-and-azide-free-ab212857--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img91124.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TAGLN/Transgelin antibody [TAGLN/247] - BSA and Azide free (AB212857)")
Publications
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- Cardiovascular drugs and therapy 36:575-5882021CHOP Increases TRIB3-Dependent miR-208 Expression to Potentiate Vascular Smooth Muscle Cell Proliferation and Migration by Downregulating TIMP3 in Atherosclerosis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRui Chen,Yan Zhang,Chunyan ZhaoPubMed 33856595
- Arteriosclerosis, thrombosis, and vascular biology 41:1428-14452021Knockout of the Gene Inhibits Neointima Formation in a Mouse Model of Vascular Injury.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXingli Xu (徐兴丽),Xinghua Xu (徐兴华),Yang Mao (毛洋),Lin Lu (卢琳),Jing Ma (马静),Tengfei Zheng (郑腾飞),Jie Zhang (张杰),Meng Zhang (章萌),Linlin Meng (孟霖霖),Lianyue Ma (马连越),Jing Cheng (程晶),Wenqiang Chen (陈文强),Hong Jiang (姜虹),Yun Zhang (张运),Cheng Zhang (张澄)PubMed 33626912
- Experimental and therapeutic medicine 21:3222021Effects of shRNA-mediated silencing of PDE5A3 on intracellular cGMP and free Ca levels and human prostate smooth muscle cell proliferation from benign prostatic hyperplasia.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZheng Xu,Yuzheng Ge,Ke Jiang,Luwei Xu,Jiageng Zhu,Changcheng Zhou,Liuhua Zhou,Ruipeng JiaPubMed 33732295
- Journal of the American Heart Association 9:e0141032019Drug-Eluting Stent Targeting Sp-1-Attenuated Restenosis by Engaging YAP-Mediated Vascular Smooth Muscle Cell Phenotypic Modulation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChen Huang,Jie Zhao,Yuelin ZhuPubMed 31880978
- Arteriosclerosis, thrombosis, and vascular biology 38:1878-18892018Diabetic Vascular Calcification Mediated by the Collagen Receptor Discoidin Domain Receptor 1 via the Phosphoinositide 3-Kinase/Akt/Runt-Related Transcription Factor 2 Signaling Axis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMarsel Lino,Mark H Wan,Antonio S Rocca,David Ngai,Navid Shobeiri,Guangpei Hou,Chunxi Ge,Renny T Franceschi,Michelle P BendeckPubMed 29930002
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- Recombinant Full Length Protein corresponding to Human TAGLN. Database link Q01995
Reactivity data
Select an application| Flow Cyt | ICC/IF | IHC-P | |
|---|---|---|---|
| Human | Expected | Expected | Tested |
| Mouse | Predicted | Predicted | Predicted |
| Rat | Predicted | Predicted | Predicted |
| Cow | Predicted | Predicted | Predicted |
| Pig | Predicted | Predicted | Predicted |
| Rabbit | Predicted | Predicted | Predicted |
Flow Cyt
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1 µg for 106 Cells | Notes (in 0.1 ml). Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Rabbit, Cow, Pig | Dilution info - | Notes - |
ICC/IF
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1-2 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Rabbit, Cow, Pig | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.5-1 µg/mL | Notes (Primary incubation for 30 minutes at RT) Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Rabbit, Cow, Pig | Dilution info - | Notes - |
Associated Products
Select an associated product type
6 products for Alternative Product
1 product for Alternative Version
Target data
Function
Actin cross-linking/gelling protein (By similarity). Involved in calcium interactions and contractile properties of the cell that may contribute to replicative senescence.
Alternative names
SM22, WS3-10, TAGLN, Transgelin, 22 kDa actin-binding protein, Protein WS3-10, Smooth muscle protein 22-alpha, SM22-alpha
Recommended products
Mouse Monoclonal TAGLN/Transgelin antibody. Carrier free. Suitable for Flow Cyt, ICC/IF, IHC-P and reacts with Human samples. Cited in 5 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human TAGLN.
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- Recombinant Full Length Protein corresponding to Human TAGLN. Database link Q01995
- Carrier free
- Yes
- Clone number
- TAGLN/247
- Purification technique
- Affinity purification Protein G
- Light chain type
- kappa
- Concentration
- Purification notes
ab212857 is purified from Bioreactor Concentrate by Protein A/G.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
TAGLN also known as Transgelin or SM22-alpha is a protein with a molecular weight of approximately 22 kDa. Structurally it belongs to the calponin family and contains an ACT domain which is critical for its actin-binding ability. It is expressed abundantly in smooth muscle cells where it stabilizes cytoskeletal dynamics by interacting with actin filaments. TAGLN also shows expression in fibroblasts and myofibroblasts supporting structural integrity and cellular movement.
- Biological function summary
The regulatory involvement of TAGLN in smooth muscle contraction influences cellular activities essential for vascular function. This protein modulates remodeling and differentiation within smooth muscle cells. It can associate with actin and calponin to form complexes that regulate the stability of cytoskeletal structures ensuring effective cellular responses to mechanical stimuli. By linking signal transduction to cytoskeletal mechanics TAGLN facilitates the maintenance of cell shape and tension.
- Pathways
TAGLN plays a role in the regulation of the RhoA/Rho kinase pathway contributing to actin cytoskeleton organization and smooth muscle contraction. Additionally it is involved in the WNT signaling pathway influencing cell proliferation and differentiation. In these pathways TAGLN works with proteins like RhoA and beta-catenin to transmit signals from the extracellular environment to the nucleus impacting gene expression and cellular responses.
- Associated diseases and disorders
Disruptions in TAGLN expression associate with vascular diseases and certain cancers. Downregulation or altered expression patterns can lead to smooth muscle cell dysfunction contributing to atherosclerosis. Similarly TAGLN interacts with proteins such as fibronectin and MMP2 in cancer where its altered expression could promote metastasis and tumor progression. Understanding these connections highlights TAGLN's potential as a target for therapeutic interventions in these diseases.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TAGLN/Transgelin antibody [TAGLN/247] - BSA and Azide free (ab212857), expandable thumbnail](https://content.abcam.com/products/images/tagln-transgelin-antibody-tagln-247-bsa-and-azide-free-ab212857--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img9865.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TAGLN/Transgelin antibody [TAGLN/247] - BSA and Azide free (ab212857)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TAGLN/Transgelin antibody [TAGLN/247] - BSA and Azide free (ab212857)
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human colon carcinoma tissue labeling SM22 alpha with ab212857 at 1μg/ml.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TAGLN/Transgelin antibody [TAGLN/247] - BSA and Azide free (ab212857), expandable thumbnail](https://content.abcam.com/products/images/tagln-transgelin-antibody-tagln-247-bsa-and-azide-free-ab212857--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img91124.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TAGLN/Transgelin antibody [TAGLN/247] - BSA and Azide free (ab212857)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TAGLN/Transgelin antibody [TAGLN/247] - BSA and Azide free (ab212857)
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human colon carcinoma tissue labeling SM22 alpha with ab212857 at 1μg/ml.
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