AB212857

Anti-TAGLN/Transgelin antibody [TAGLN/247] - BSA and Azide free

Name: Anti-TAGLN/Transgelin antibody [TAGLN/247] - BSA and Azide free (ab212857) | Abcam
Brand: Abcam Limited
SKU: AB212857
Price: 905 USD
Availability: InStock

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(8 Publications)

Mouse Monoclonal TAGLN/Transgelin antibody. Carrier free. Suitable for Flow Cyt, ICC/IF, IHC-P and reacts with Human samples. Cited in 8 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human TAGLN.

View Alternative Names

SM22, WS3-10, TAGLN, Transgelin, 22 kDa actin-binding protein, Protein WS3-10, Smooth muscle protein 22-alpha, SM22-alpha

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TAGLN/Transgelin antibody [TAGLN/247] - BSA and Azide free (AB212857)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TAGLN/Transgelin antibody [TAGLN/247] - BSA and Azide free (AB212857)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human colon carcinoma tissue labeling SM22 alpha with ab212857 at 1μg/ml.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TAGLN/Transgelin antibody [TAGLN/247] - BSA and Azide free (AB212857)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human colon carcinoma tissue labeling SM22 alpha with ab212857 at 1μg/ml.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

TAGLN/247

Isotype

IgG1

Light chain type

kappa

Carrier free

Yes

Reacts with

Human

Applications

ICC/IF, IHC-P, Flow Cyt

applications

Immunogen

Recombinant Full Length Protein corresponding to Human TAGLN.

Q01995

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "1 µg for 10^6 Cells", "FlowCyt-species-notes": "(in 0.1 ml).", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "1-2 µg/mL", "ICCIF-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "0.5-1 µg/mL", "IHCP-species-notes": "(Primary incubation for 30 minutes at RT) Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." }, "Mouse": { "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Cow": { "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Pig": { "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rabbit": { "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Purification notes

ab212857 is purified from Bioreactor Concentrate by Protein A/G.

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TAGLN also known as Transgelin or SM22-alpha is a protein with a molecular weight of approximately 22 kDa. Structurally it belongs to the calponin family and contains an ACT domain which is critical for its actin-binding ability. It is expressed abundantly in smooth muscle cells where it stabilizes cytoskeletal dynamics by interacting with actin filaments. TAGLN also shows expression in fibroblasts and myofibroblasts supporting structural integrity and cellular movement.

Biological function summary

The regulatory involvement of TAGLN in smooth muscle contraction influences cellular activities essential for vascular function. This protein modulates remodeling and differentiation within smooth muscle cells. It can associate with actin and calponin to form complexes that regulate the stability of cytoskeletal structures ensuring effective cellular responses to mechanical stimuli. By linking signal transduction to cytoskeletal mechanics TAGLN facilitates the maintenance of cell shape and tension.

Pathways

TAGLN plays a role in the regulation of the RhoA/Rho kinase pathway contributing to actin cytoskeleton organization and smooth muscle contraction. Additionally it is involved in the WNT signaling pathway influencing cell proliferation and differentiation. In these pathways TAGLN works with proteins like RhoA and beta-catenin to transmit signals from the extracellular environment to the nucleus impacting gene expression and cellular responses.

Disruptions in TAGLN expression associate with vascular diseases and certain cancers. Downregulation or altered expression patterns can lead to smooth muscle cell dysfunction contributing to atherosclerosis. Similarly TAGLN interacts with proteins such as fibronectin and MMP2 in cancer where its altered expression could promote metastasis and tumor progression. Understanding these connections highlights TAGLN's potential as a target for therapeutic interventions in these diseases.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Actin cross-linking/gelling protein (By similarity). Involved in calcium interactions and contractile properties of the cell that may contribute to replicative senescence.

See full target information TAGLN

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Publications (8)

Recent publications for all applications. Explore the full list and refine your search

Journal of cellular physiology 239:e31251 PubMed38634445

2024

KLF13 promotes VSMCs phenotypic dedifferentiation by directly binding to the SM22α promoter.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaofan Yuan,Chuan Jiang,Yuzhou Xue,Fuqiang Guo,Minghao Luo,Lei Guo,Yang Gao,Tongling Yuan,Hui Xu,Hong Chen

Cardiovascular drugs and therapy 36:575-588 PubMed33856595

2021

CHOP Increases TRIB3-Dependent miR-208 Expression to Potentiate Vascular Smooth Muscle Cell Proliferation and Migration by Downregulating TIMP3 in Atherosclerosis.

Applications

Unspecified application

Species

Unspecified reactive species

Rui Chen,Yan Zhang,Chunyan Zhao

Arteriosclerosis, thrombosis, and vascular biology 41:1428-1445 PubMed33626912

2021

Knockout of the Gene Inhibits Neointima Formation in a Mouse Model of Vascular Injury.

Applications

Unspecified application

Species

Unspecified reactive species

Xingli Xu (徐兴丽),Xinghua Xu (徐兴华),Yang Mao (毛洋),Lin Lu (卢琳),Jing Ma (马静),Tengfei Zheng (郑腾飞),Jie Zhang (张杰),Meng Zhang (章萌),Linlin Meng (孟霖霖),Lianyue Ma (马连越),Jing Cheng (程晶),Wenqiang Chen (陈文强),Hong Jiang (姜虹),Yun Zhang (张运),Cheng Zhang (张澄)

Experimental and therapeutic medicine 21:322 PubMed33732295

2021

Effects of shRNA-mediated silencing of PDE5A3 on intracellular cGMP and free Ca levels and human prostate smooth muscle cell proliferation from benign prostatic hyperplasia.

Applications

Unspecified application

Species

Unspecified reactive species

Zheng Xu,Yuzheng Ge,Ke Jiang,Luwei Xu,Jiageng Zhu,Changcheng Zhou,Liuhua Zhou,Ruipeng Jia

Journal of the American Heart Association 9:e014103 PubMed31880978

2019

Drug-Eluting Stent Targeting Sp-1-Attenuated Restenosis by Engaging YAP-Mediated Vascular Smooth Muscle Cell Phenotypic Modulation.

Applications

Unspecified application

Species

Unspecified reactive species

Chen Huang,Jie Zhao,Yuelin Zhu

American journal of physiology. Heart and circulat 317:H541-H551 PubMed31298560

2019

Drug-eluting stent specifically designed to target vascular smooth muscle cell phenotypic modulation attenuated restenosis through the YAP pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Chen Huang,Wenwen Zhang,Yuelin Zhu

Arteriosclerosis, thrombosis, and vascular biology 38:1878-1889 PubMed29930002

2018

Diabetic Vascular Calcification Mediated by the Collagen Receptor Discoidin Domain Receptor 1 via the Phosphoinositide 3-Kinase/Akt/Runt-Related Transcription Factor 2 Signaling Axis.

Applications

Unspecified application

Species

Unspecified reactive species

Marsel Lino,Mark H Wan,Antonio S Rocca,David Ngai,Navid Shobeiri,Guangpei Hou,Chunxi Ge,Renny T Franceschi,Michelle P Bendeck

Oncology letters 15:7279-7290 PubMed29731886

2018

Cluster of differentiation 147 mediates chemoresistance in breast cancer by affecting vacuolar H-ATPase expression and activity.

Applications

Unspecified application

Species

Unspecified reactive species

Yehong Kuang,Shouman Wang,Lili Tang,Jian Hai,Guojiao Yan,Liqiu Liao

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-TAGLN/Transgelin antibody [TAGLN/247] - BSA and Azide free

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TAGLN/Transgelin antibody [TAGLN/247] - BSA and Azide free (AB212857)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TAGLN/Transgelin antibody [TAGLN/247] - BSA and Azide free (AB212857)

Key facts

Host species

Clonality

Clone number

Isotype

Light chain type

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (8)

Product promise