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Rabbit Recombinant Monoclonal TAK1 antibody. Suitable for WB and reacts with Drosophila melanogaster samples. Cited in 1 publication.

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Images

Western blot - Anti-TAK1 antibody [EPR22903-57] (AB239353), expandable thumbnail
  • Western blot - Anti-TAK1 antibody [EPR22903-57] (AB239353), expandable thumbnail

Publications

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PIPWB
Drosophila melanogaster
Not recommended
Not recommended
Tested

Not recommended
Not recommended

Species

Drosophila melanogaster

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Drosophila melanogaster

Dilution info

-

Notes

-

Tested
Tested

Species

Drosophila melanogaster

Dilution info

1/1000

Notes

-

Associated Products

Select an associated product type

5 products for Alternative Product

Target data

Function

Component of a protein kinase signal transduction cascade. Mediator of TGF-beta signal transduction. Responsible for activation of the JNK MAPK pathway (basket, bsk and hemipterous, hep) in response to LPS. Component of the NF-kappa-B pathway; relish-mediated JNK inhibition involves proteasomal degradation of Tak1; certain targets of Relish that are induced during immune responses may facilitate destruction of Tak1 and switch off the JNK cascade. Participates in diverse roles such as control of cell shape and regulation of apoptosis.

Alternative names

CG18492, CG18492, Tak1, Mitogen-activated protein kinase kinase kinase 7, TGF-beta-activated kinase 1, dTAK1

Recommended products

Rabbit Recombinant Monoclonal TAK1 antibody. Suitable for WB and reacts with Drosophila melanogaster samples. Cited in 1 publication.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EPR22903-57

Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

2 product images

  • Western blot - Anti-TAK1 antibody [EPR22903-57] (ab239353), expandable thumbnail

    Western blot - Anti-TAK1 antibody [EPR22903-57] (ab239353)

    Blocking/Diluting buffer: 5% NFDM/TBST

    All lanes: Western blot - Anti-TAK1 antibody [EPR22903-57] (ab239353) at 1/1000 dilution

    All lanes: Drosophila schneider 2 (S2) cell whole cell lysate at 10 µL

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Predicted band size: 67 kDa

    Observed band size: 85 kDa

    Exposure time: 26s

    Blocking/Diluting buffer: 5% NFDM/TBST

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab239353).

  • Western blot - Anti-TAK1 antibody [EPR22903-57] (ab239353), expandable thumbnail

    Western blot - Anti-TAK1 antibody [EPR22903-57] (ab239353)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    This blot was developed using a higher sensitivity ECL substrate.
    The lysates were kindly provided by an anonymous collaborator.

    Exposure time: 3 minutes

    All lanes: Western blot - Anti-TAK1 antibody [EPR22903-57] (ab239353) at 1/1000 dilution

    Lane 1: Wild type Drosophila lysate at 20 µg

    Lane 2: Drosophila Tak1[2] mutant (with a point mutation resulting in a 53aa truncated protein) lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 67 kDa

    Observed band size: 85 kDa

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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