Rabbit Recombinant Monoclonal TAK1 antibody. Suitable for WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Human, Mouse, Rat samples. Cited in 66 publications.
Images
![Western blot - Anti-TAK1 antibody [EPR5984] (AB109526), expandable thumbnail](https://content.abcam.com/products/images/tak1-antibody-epr5984-ab109526--western-blot-img6879.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-TAK1 antibody [EPR5984] (AB109526)")
![Immunocytochemistry/ Immunofluorescence - Anti-TAK1 antibody [EPR5984] (AB109526), expandable thumbnail](https://content.abcam.com/products/images/tak1-antibody-epr5984-ab109526--immunocytochemistry-immunofluorescence-img121771.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-TAK1 antibody [EPR5984] (AB109526)")
![Flow Cytometry (Intracellular) - Anti-TAK1 antibody [EPR5984] (AB109526), expandable thumbnail](https://content.abcam.com/products/images/tak1-antibody-epr5984-ab109526--flow-cytometry-intracellular-img139044.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-TAK1 antibody [EPR5984] (AB109526)")
![Western blot - Anti-TAK1 antibody [EPR5984] (AB109526), expandable thumbnail](https://content.abcam.com/products/images/tak1-antibody-epr5984-ab109526--western-blot-img105233.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-TAK1 antibody [EPR5984] (AB109526)")
![Western blot - Anti-TAK1 antibody [EPR5984] (AB109526), expandable thumbnail](https://content.abcam.com/products/images/tak1-antibody-epr5984-ab109526--western-blot-img136467.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-TAK1 antibody [EPR5984] (AB109526)")
Publications
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- Scientific reports 13:211062023Application of human platelet lysate in chondrocyte expansion promotes chondrogenic phenotype and slows senescence progression via BMP-TAK1-p38 pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNarong Chitchongyingcharoen,Tulyapruek Tawonsawatruk,Jitrada Phetfong,Wrattya Aroontanee,Aungkura SupokawejPubMed 38036641
- Nature communications 14:66992023Broad-spectrum kinome profiling identifies CDK6 upregulation as a driver of lenvatinib resistance in hepatocellular carcinoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesCarmen Oi Ning Leung,Yang Yang,Rainbow Wing Hei Leung,Karl Kam Hei So,Hai Jun Guo,Martina Mang Leng Lei,Gregory Kenneth Muliawan,Yuan Gao,Qian Qian Yu,Jing Ping Yun,Stephanie Ma,Qian Zhao,Terence Kin Wah LeePubMed 37872167
- Aging and disease 14:1799-18172023TAK1 Improves Cognitive Function via Suppressing RIPK1-Driven Neuronal Apoptosis and Necroptosis in Rats with Chronic Hypertension.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJing Yang,Pei Sun,Xiangming Xu,Xiaolu Liu,Linfang Lan,Ming Yi,Chi Xiao,Ruichen Ni,Yuhua FanPubMed 37196118
- Drug design, development and therapy 17:2287-23012023Dispelling Dampness, Relieving Turbidity and Dredging Collaterals Decoction, Attenuates Potassium Oxonate-Induced Hyperuricemia in Rat Models.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHai-Bo Liu,Min Yang,Wan Li,Ting Luo,Yang Wu,Xiang-Yu Huang,Yao-Lei Zhang,Tao Liu,Yong LuoPubMed 37551408
- Cell biology international 47:1198-12082023RNF150 suppresses papillary thyroid carcinoma with ASK1 ubiquitination presenting a direct target via inactivating p38 signaling axis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWei Deng,Jing Wu,Wei Zheng,Qian Wang,Dongling Li,Hongyu KuangPubMed 36950779
- JHEP reports : innovation in hepatology 5:1006952023TAK1 deficiency promotes liver injury and tumorigenesis via ferroptosis and macrophage cGAS-STING signalling.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWantong Su,Weicheng Gao,Rui Zhang,Qi Wang,Lei Li,Qingfa Bu,Zibo Xu,Zheng Liu,Mingming Wang,Yaqing Zhu,Guoping Wu,Haoming Zhou,Xun Wang,Ling LuPubMed 36968217
- World journal of gastroenterology 28:5420-54432022Lentivirus-mediated short hairpin RNA interference of CENPK inhibits growth of colorectal cancer cells with overexpression of Cullin 4A.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXian Li,Yi-Ru Han,Xuefeng Xuefeng,Yong-Xiang Ma,Guo-Sheng Xing,Zhi-Wen Yang,Zhen Zhang,Lin Shi,Xin-Lin WuPubMed 36312839
- Phytotherapy research : PTR 37:731-7422022Effect of curcumin on regulatory B cells in chronic colitis mice involving TLR/MyD88 signaling pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJie Huang,Tiantian Wu,Youbao Zhong,Jiaqi Huang,Zengping Kang,Bugao Zhou,Haimei Zhao,Duanyong LiuPubMed 36196887
- Frontiers in microbiology 13:9891832022Toll-like receptor 2 signaling pathway activation contributes to a highly efficient inflammatory response in Japanese encephalitis virus-infected mouse microglial cells by proteomics.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGuanyu Zhao,Yan Gao,Jiaqi Zhang,He Zhang,Changzhan Xie,Fulong Nan,Sheng Feng,Zhuo Ha,Chenghui Li,Xiangyu Zhu,Zhuoxin Li,Ping Zhang,Ying Zhang,Huijun Lu,Ningyi JinPubMed 36171749
- International journal of molecular sciences 23:2022Multi-Omics Studies Unveil Extraciliary Functions of BBS10 and Show Metabolic Aberrations Underlying Renal Disease in Bardet-Biedl Syndrome.Applications:Unspecified applicationReactive species:Unspecified reactive speciesEmanuela Marchese,Marianna Caterino,Roberta Fedele,Francesca Pirozzi,Armando Cevenini,Neha Gupta,Diego Ingrosso,Alessandra Perna,Giovambattista Capasso,Margherita Ruoppolo,Miriam ZacchiaPubMed 36012682
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.05% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 0.1% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | WB | ICC/IF | Flow Cyt (Intra) | IHC-P | |
|---|---|---|---|---|---|
| Human | Not recommended | Tested | Tested | Tested | Tested |
| Mouse | Not recommended | Tested | Expected | Expected | Expected |
| Rat | Not recommended | Tested | Expected | Expected | Expected |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval via the microwave method before commencing with IHC staining protocol. |
Species Mouse | Dilution info - | Notes - |
Species Rat | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000 - 1/10000 | Notes - |
Species Mouse | Dilution info 1/1000 | Notes - |
Species Rat | Dilution info 1/1000 | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/50 - 1/100 | Notes (Heat to 98°C, allow to cool for 10-20 minutes) Perform heat-mediated antigen retrieval via the microwave method before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
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Target data
Function
Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway (PubMed:10094049, PubMed:11460167, PubMed:12589052, PubMed:16845370, PubMed:16893890, PubMed:21512573, PubMed:8663074, PubMed:9079627). Plays an important role in the cascades of cellular responses evoked by changes in the environment (PubMed:10094049, PubMed:11460167, PubMed:12589052, PubMed:16845370, PubMed:16893890, PubMed:21512573, PubMed:8663074, PubMed:9079627). Mediates signal transduction of TRAF6, various cytokines including interleukin-1 (IL-1), transforming growth factor-beta (TGFB), TGFB-related factors like BMP2 and BMP4, toll-like receptors (TLR), tumor necrosis factor receptor CD40 and B-cell receptor (BCR) (PubMed:16893890, PubMed:9079627). Once activated, acts as an upstream activator of the MKK/JNK signal transduction cascade and the p38 MAPK signal transduction cascade through the phosphorylation and activation of several MAP kinase kinases like MAP2K1/MEK1, MAP2K3/MKK3, MAP2K6/MKK6 and MAP2K7/MKK7 (PubMed:11460167, PubMed:8663074). These MAP2Ks in turn activate p38 MAPKs and c-jun N-terminal kinases (JNKs); both p38 MAPK and JNK pathways control the transcription factors activator protein-1 (AP-1) (PubMed:11460167, PubMed:12589052, PubMed:8663074). Independently of MAP2Ks and p38 MAPKs, acts as a key activator of NF-kappa-B by promoting activation of the I-kappa-B-kinase (IKK) core complex (PubMed:12589052, PubMed:8663074). Mechanistically, recruited to polyubiquitin chains of RIPK2 and IKBKG/NEMO via TAB2/MAP3K7IP2 and TAB3/MAP3K7IP3, and catalyzes phosphorylation and activation of IKBKB/IKKB component of the IKK complex, leading to NF-kappa-B activation (PubMed:10094049, PubMed:11460167). In osmotic stress signaling, plays a major role in the activation of MAPK8/JNK1, but not that of NF-kappa-B (PubMed:16893890). Promotes TRIM5 capsid-specific restriction activity (PubMed:21512573). Phosphorylates RIPK1 at 'Ser-321' which positively regulates RIPK1 interaction with RIPK3 to promote necroptosis but negatively regulates RIPK1 kinase activity and its interaction with FADD to mediate apoptosis (By similarity). Phosphorylates STING1 in response to cGAMP-activation, promoting association between STEEP1 and STING1 and STING1 translocation to COPII vesicles (PubMed:37832545).
Alternative names
TAK1, MAP3K7, Mitogen-activated protein kinase kinase kinase 7, Transforming growth factor-beta-activated kinase 1, TGF-beta-activated kinase 1
Recommended products
Rabbit Recombinant Monoclonal TAK1 antibody. Suitable for WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Human, Mouse, Rat samples. Cited in 66 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.05% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 0.1% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR5984
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Stable for 12 months at -20°C
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
TAK1 also known as MAP3K7 (Mitogen-activated protein kinase kinase kinase 7) is an important protein kinase that weighs approximately 63 kDa. This protein is expressed in various tissues and cells including HEK 293T cells. TAK1 plays a mechanical role as a part of the MAPK signaling cascade. It phosphorylates and activates downstream kinases which is key to transmitting cellular signals that regulate responses to external stimuli like cytokines and stress.
- Biological function summary
TAK1 is involved in several cellular processes necessary for maintaining balance and responding to stress. TAK1 forms a complex with proteins like TAB1 TAB2 and TAB3 which are important for its activation and signaling function. This complex facilitates TAK1's involvement in inflammation and immune response indicating its significance in mediating cellular survival and apoptosis.
- Pathways
TAK1 operates within the NF-κB and MAPK pathways two critical routes for cellular response to inflammation and stress. In the NF-κB pathway TAK1 activates IKK which triggers the degradation of IκB freeing NF-κB to move into the nucleus and activate transcription. In the MAPK pathway TAK1 directly influences JNK and p38 cascades revealing regulatory connections to proteins like MEK and ERK.
- Associated diseases and disorders
TAK1 has been linked to conditions such as cancer and inflammatory diseases. In cancers aberrant TAK1 activity can lead to enhanced cell proliferation and survival. Moreover inflammation-related disorders can arise from malfunctioning TAK1 signaling demonstrating its connection to proteins like TNF receptor-associated factors which are involved in inflammatory responses. Understanding TAK1's role in these disorders may pave the way for developing potential therapeutic interventions.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
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8 product images
![Western blot - Anti-TAK1 antibody [EPR5984] (ab109526), expandable thumbnail](https://content.abcam.com/products/images/tak1-antibody-epr5984-ab109526--western-blot-img6879.jpg "Western blot - Anti-TAK1 antibody [EPR5984] (ab109526)")
Western blot - Anti-TAK1 antibody [EPR5984] (ab109526)
Lane 1: Wild-type HAP1 cell lysate (20 μg)
Lane 2: TAK1 knockout HAP1 cell lysate (20 μg)
Lane 3: SHSY5Y cell lysate (20 μg)
Lane 4: A431 cell lysate (20 μg)
Lanes 1 - 4: Merged signal (red and green). Green - ab109526 observed at 72 kDa. Red - loading control, Anti-GAPDH antibody [6C5] - Loading Control ab8245, observed at 37 kDa.
ab109526 was shown to specifically react with TAK1 when TAK1 knockout samples were used. Wild-type and TAK1 knockout samples were subjected to SDS-PAGE. ab109526 and Anti-GAPDH antibody [6C5] - Loading Control ab8245 (loading control to GAPDH) were diluted 1/1000 and 1/2000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.All lanes: Western blot - Anti-TAK1 antibody [EPR5984] (ab109526)
Predicted band size: 67 kDa
![Immunocytochemistry/ Immunofluorescence - Anti-TAK1 antibody [EPR5984] (ab109526), expandable thumbnail](https://content.abcam.com/products/images/tak1-antibody-epr5984-ab109526--immunocytochemistry-immunofluorescence-img121771.jpg "Immunocytochemistry/ Immunofluorescence - Anti-TAK1 antibody [EPR5984] (ab109526)")
Immunocytochemistry/ Immunofluorescence - Anti-TAK1 antibody [EPR5984] (ab109526)
ab109526 staining TAK1 in wild-type HAP1 cells (top panel) and TAK1 knockout HAP1 cells (bottom panel). The cells were fixed with 4% formaldehyde (10min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab109526 at 1/1000 dilution and Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 at 1/250 dilution (shown in pseudo colour red) overnight at +4°C, followed by a further incubation at room temperature for 1h with a goat secondary antibody to Rabbit IgG (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 2 μg/ml (shown in green). Nuclear DNA was labelled in blue with DAPI.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
![Flow Cytometry (Intracellular) - Anti-TAK1 antibody [EPR5984] (ab109526), expandable thumbnail](https://content.abcam.com/products/images/tak1-antibody-epr5984-ab109526--flow-cytometry-intracellular-img139044.jpg "Flow Cytometry (Intracellular) - Anti-TAK1 antibody [EPR5984] (ab109526)")
Flow Cytometry (Intracellular) - Anti-TAK1 antibody [EPR5984] (ab109526)
Intracellular Flow Cytometry analysis of A431 (human epidermoid carcinoma) cells labeling TAK1 with unpurified ab109526 at 1/20 dilution (10ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488) (1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) was used as the unlabeled control.
![Western blot - Anti-TAK1 antibody [EPR5984] (ab109526), expandable thumbnail](https://content.abcam.com/products/images/tak1-antibody-epr5984-ab109526--western-blot-img105233.jpg "Western blot - Anti-TAK1 antibody [EPR5984] (ab109526)")
Western blot - Anti-TAK1 antibody [EPR5984] (ab109526)
All lanes: Western blot - Anti-TAK1 antibody [EPR5984] (ab109526) at 1/1000 dilution
Lane 1: K562 cell lysate at 10 µg
Lane 2: HeLa cell lysate at 10 µg
Lane 3: A431 cell lysate at 10 µg
SecondaryAll lanes: HRP labelled Goat anti-Rabbit IgG at 1/2000 dilution
Predicted band size: 67 kDa
Observed band size: 75 kDa
![Western blot - Anti-TAK1 antibody [EPR5984] (ab109526), expandable thumbnail](https://content.abcam.com/products/images/tak1-antibody-epr5984-ab109526--western-blot-img136467.jpg "Western blot - Anti-TAK1 antibody [EPR5984] (ab109526)")
Western blot - Anti-TAK1 antibody [EPR5984] (ab109526)
Lanes 1- 2: Merged signal (red and green). Green - ab109526 observed at 72 kDa. Red - Anti-GAPDH antibody [6C5] - Loading Control (Anti-GAPDH antibody [6C5] - Loading Control ab8245) observed at 37 kDa.ab109526 was shown to react with TAK1 in wild-type HEK-293T cells in western blot. Loss of signal was observed when knockout cell line Human MAP3K7 (TAK1) knockout HEK-293T cell line ab266555 (knockout cell lysate Human MAP3K7 (TAK1) knockout HEK-293T cell lysate ab256984) was used. Wild-type HEK-293T and MAP3K7 knockout HEK-293T cell lysates were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab109526 and Anti-GAPDH antibody [6C5] - Loading Control (Anti-GAPDH antibody [6C5] - Loading Control ab8245) overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye®800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye®680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes: Western blot - Anti-TAK1 antibody [EPR5984] (ab109526) at 1/1000 dilution
Lane 1: Wild-type HEK-293T cell lysate at 20 µg
Lane 2: MAP3K7 knockout HEK-293T cell lysate at 20 µg
Lane 2: Western blot - Human MAP3K7 (TAK1) knockout HEK-293T cell line (Human MAP3K7 (TAK1) knockout HEK-293T cell line ab266555)
Performed under reducing conditions.
Predicted band size: 35 kDa, 36 kDa, 43 kDa, 47 kDa, 60 kDa, 67 kDa, 74 kDa
Observed band size: 36 kDa, 60 kDa, 72 kDa, 95 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TAK1 antibody [EPR5984] (ab109526), expandable thumbnail](https://content.abcam.com/products/images/tak1-antibody-epr5984-ab109526--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img15466.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TAK1 antibody [EPR5984] (ab109526)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TAK1 antibody [EPR5984] (ab109526)
ab109526, at a 1/50 dilution, staining TAK1 in Formalin/PFA-fixed paraffin-embedded Human brain tissue, by Immunohistochemistry.
Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol.
![Western blot - Anti-TAK1 antibody [EPR5984] (ab109526), expandable thumbnail](https://content.abcam.com/products/images/tak1-antibody-epr5984-ab109526--western-blot-img444560.jpg "Western blot - Anti-TAK1 antibody [EPR5984] (ab109526)")
Western blot - Anti-TAK1 antibody [EPR5984] (ab109526)
All lanes: Western blot - Anti-TAK1 antibody [EPR5984] (ab109526) at 1/1000 dilution
Lane 1: Mouse brain tissue lysate at 20 µg
Lane 2: Mouse heart tissue lysate at 20 µg
Lane 3: Mouse spleen tissue lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 75 kDa
Exposure time: 48s
![Western blot - Anti-TAK1 antibody [EPR5984] (ab109526), expandable thumbnail](https://content.abcam.com/products/images/tak1-antibody-epr5984-ab109526--western-blot-img444561.jpg "Western blot - Anti-TAK1 antibody [EPR5984] (ab109526)")
Western blot - Anti-TAK1 antibody [EPR5984] (ab109526)
All lanes: Western blot - Anti-TAK1 antibody [EPR5984] (ab109526) at 1/1000 dilution
Lane 1: Rat brain tissue lysate at 20 µg
Lane 2: Rat kidney tissue lysate at 20 µg
Lane 3: Rat spleen tissue lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 75 kDa
Exposure time: 180s
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TAK1 antibody [EPR5984] (ab109526), expandable thumbnail](https://content.abcam.com/products/images/tak1-antibody-epr5984-ab109526--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img15466.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TAK1 antibody [EPR5984] (ab109526)")
![Western blot - Anti-TAK1 antibody [EPR5984] (ab109526), expandable thumbnail](https://content.abcam.com/products/images/tak1-antibody-epr5984-ab109526--western-blot-img444560.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-TAK1 antibody [EPR5984] (ab109526)")
![Western blot - Anti-TAK1 antibody [EPR5984] (ab109526), expandable thumbnail](https://content.abcam.com/products/images/tak1-antibody-epr5984-ab109526--western-blot-img444561.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-TAK1 antibody [EPR5984] (ab109526)")
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