JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB308573

Anti-Tal1 antibody [HL1703] - BSA and Azide free

Be the first to review this product! Submit a review

|

(0 Publication)

Rabbit Recombinant Monoclonal TAL1 antibody. Carrier free. Suitable for WB and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human TAL1.

View Alternative Names

BHLHA17, SCL, TCL5, TAL1, T-cell acute lymphocytic leukemia protein 1, TAL-1, Class A basic helix-loop-helix protein 17, Stem cell protein, T-cell leukemia/lymphoma protein 5, bHLHa17

1 Images
Western blot - Anti-Tal1 antibody [HL1703] - BSA and Azide free (AB308573)
  • WB

Supplier Data

Western blot - Anti-Tal1 antibody [HL1703] - BSA and Azide free (AB308573)

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with TAL1 antibody HL1703 (ab308573) diluted at 1/1000. A HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

All lanes:

Western blot - Anti-Tal1 antibody [HL1703] - BSA and Azide free (ab308573) at 1/1000 dilution

Lane 1:

Jurkat cell lysate at 30 µg

Lane 2:

HeLa cell lysate at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 34.271 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

HL1703

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB

applications

Immunogen

Synthetic Peptide within Human TAL1. The exact immunogen used to generate this antibody is proprietary information.

P17542

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" } } }
style
left-column

Product details

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Constituents: 100% PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The protein Tal1 also known as T-cell acute lymphocytic leukemia protein 1 and SCL (stem cell leukemia) is a transcription factor of about 34 kDa. It plays a role in the regulation of hematopoiesis. Tal1 is expressed in hematopoietic cells and tissues like bone marrow thymus and fetal liver. It binds to specific DNA sequences to regulate gene expression essential for the development and function of blood cells.
Biological function summary

The Tal1 protein controls the differentiation and proliferation of hematopoietic stem cells and progenitor cells. It participates as a part of a multiprotein complex involving other transcription factors and cofactors such as GATA-1 and LMO2 which modulates gene expression during blood cell lineage commitment. Through these interactions Tal1 influences both myeloid and lymphoid lineage differentiation.

Pathways

Tal1 acts as an important regulator in the hematopoietic development pathway and is critical for the endothelial-to-hematopoietic transition. This transition is supported by interaction with the E2A proteins which stabilize the formation of the transcription complex needed for lineage-specific gene regulation. Tal1 also plays a role in the VEGF signaling pathway impacting vascular and blood development processes.

Tal1 significantly associates with T-cell acute lymphoblastic leukemia (T-ALL) and some types of anemia. Its deregulation often through chromosomal translocations or overexpression results in aberrant hematopoiesis contributing to leukemogenesis. In T-ALL Tal1 can interact with oncogenic proteins like LMO1 and LMO2 leading to the disruption of normal T-cell development and proliferation highlighting its involvement in the disease's molecular pathology.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Implicated in the genesis of hemopoietic malignancies. It may play an important role in hemopoietic differentiation. Serves as a positive regulator of erythroid differentiation (By similarity).
See full target information TAL1
style
left-column
style
left-column
style
right-column

Complementary Products

Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB109241

Anti-GATA2 antibody [EPR2822(2)]

RabMAb|Recombinant|KO Validated

ChIP - Anti-GATA2 antibody [EPR2822(2)] (AB109241)

  • 3
  • 1
Primary Antibodies

AB76543

Anti-PU.1/Spi1 antibody [EPR3158Y]

BOND RX™ Validated|RabMAb|Recombinant

Western blot - Anti-PU.1/Spi1 antibody [EPR3158Y] (AB76543)

  • 5
  • 3
Primary Antibodies

AB133485

Anti-FLI1 antibody [EPR4646]

RabMAb|Recombinant|Advanced Validation

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FLI1 antibody [EPR4646] (AB133485)

  • 5
  • 1

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com