JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB104913

Anti-Talin 1 antibody [93E12]

Be the first to review this product! Submit a review

|

(7 Publications)

Mouse Monoclonal Talin 1 antibody. Suitable for IP, ELISA, WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 7 publications. Immunogen corresponding to Recombinant Fragment Protein within Human TLN1 aa 750-850.

View Alternative Names

KIAA1027, TLN, TLN1, Talin-1

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

93E12

Isotype

IgG1

Carrier free

No

Reacts with

Human

Applications

ELISA, IHC-P, IP, ICC/IF, WB

applications

Immunogen

Recombinant Fragment Protein within Human TLN1 aa 750-850. The exact immunogen used to generate this antibody is proprietary information.

Q9Y490

Specificity

ab104913 can be used for specific detection of the focal adhesion protein Talin 1 and does not cross react with Talin 2.

style
left-column

Alternative Products

Primary Antibodies

AB108480

Anti-Talin 1 antibody [97H6]

BOND RX™ Validated|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Talin 1 antibody [97H6] (AB108480)

  • 5
  • 1
Primary Antibodies

AB157808

Anti-Talin antibody [8D4]

Flow Cytometry (Intracellular) - Anti-Talin antibody [8D4] (AB157808)

  • 0
  • 0
Primary Antibodies

AB71333

Anti-Talin 1 antibody

BOND RX™ Validated|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Talin 1 antibody (AB71333)

  • 4
  • 4
Primary Antibodies

AB241096

Anti-Talin 1 antibody

Immunoprecipitation - Anti-Talin 1 antibody (AB241096)

  • 5
  • 1

Complementary Products

Primary Antibodies

AB32084

Anti-Paxillin antibody [Y113]

BOND RX™ Validated|RabMAb|Recombinant|KO Validated|20ul selling size

Immunocytochemistry/ Immunofluorescence - Anti-Paxillin antibody [Y113] (AB32084)

  • 5
  • 21
Primary Antibodies

AB129002

Anti-Vinculin antibody [EPR8185] - Loading Control

20ul selling size|RabMAb|Recombinant|KO Validated

Immunocytochemistry/ Immunofluorescence - Anti-Vinculin antibody [EPR8185] - Loading Control (AB129002)

  • 5
  • 21
Primary Antibodies

AB92537

Anti-RIAM antibody [EPR2806]

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RIAM antibody [EPR2806] (AB92537)

  • 0
  • 0
Primary Antibodies

AB108967

Anti-Talin 2 antibody [EPR2536(2)]

RabMAb|Recombinant

Western blot - Anti-Talin 2 antibody [EPR2536(2)] (AB108967)

  • 0
  • 0
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" } } }
style
left-column

Product details

Growth Conditions: RPMI1650 + Glu + Pen + Strep + 10% FCS @ 37C + 5%CO2.
style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
Preservative: 0.02% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Talin 1 (TLN1) is a cytoskeletal protein that mechanically links integrins to actin filaments in cells. It has a molecular weight of 270 kDa and is expressed in many tissues including muscle brain and kidney. Talin 1 contains a head domain and a tail domain which bind to integrins and actin respectively. By establishing these connections Talin 1 plays a role in cell adhesion spreading and migration. This protein also interacts with other adhesion-associated proteins reinforcing its structural function inside cells.
Biological function summary

Talin 1 is involved in facilitating integrin activation and focal adhesion formation. It is a component of a larger complex that includes proteins like vinculin and paxillin. These complexes are responsible for transmitting mechanical signals from the extracellular matrix into the intracellular environment initiating cellular responses like growth and differentiation. Talin 1 also modulates actin dynamics which contributes to changes in cell shape and motility. Its role in these processes highlights its significance in maintaining cellular architecture.

Pathways

Talin 1 participates in the integrin-mediated signaling pathway and the Rho GTPase signaling pathway. In the integrin pathway Talin 1 interacts with integrin beta subunits leading to integrin clustering and increased focal adhesion strength. In the Rho GTPase pathway Talin 1 influences actin cytoskeleton rearrangements through its interactions with proteins like RhoA and Rac1. These pathways are critical for processes such as cell proliferation and migration which are essential for proper tissue function and development.

Talin 1 has an association with cancer and cardiovascular diseases. Changes in Talin 1 expression and localization can contribute to altered cell adhesion and motility promoting cancer cell invasion and metastasis. Talin 1 has a relationship with the protein FAK (Focal Adhesion Kinase) in cancer progression. In cardiovascular diseases Talin 1 dysregulation affects cardiomyocyte adhesion and survival and it interacts with proteins such as vinculin to sustain cardiac tissue integrity. These connections highlight Talin 1's role in disease pathology.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

High molecular weight cytoskeletal protein concentrated at regions of cell-matrix and cell-cell contacts. Involved in connections of major cytoskeletal structures to the plasma membrane. With KANK1 co-organize the assembly of cortical microtubule stabilizing complexes (CMSCs) positioned to control microtubule-actin crosstalk at focal adhesions (FAs) rims.
See full target information TLN1
style
left-column

Publications (7)

Recent publications for all applications. Explore the full list and refine your search

iScience 28:111745 PubMed39898029

2025

Nuclear talin-1 provides a bridge between cell adhesion and gene expression.

Applications

Unspecified application

Species

Unspecified reactive species

Alejandro J Da Silva,Hendrik S E Hästbacka,Mikael C Puustinen,Jenny C Pessa,Jens C Luoto,Erika Sundström,Benjamin T Goult,Guillaume Jacquemet,Eva Henriksson,Lea Sistonen

Cell reports 44:115237 PubMed39883578

2025

G3BP1 ribonucleoprotein complexes regulate focal adhesion protein mobility and cell migration.

Applications

Unspecified application

Species

Unspecified reactive species

Liana C Boraas,Mengwei Hu,Pieter Martino,Lauren Thornton,Charles E Vejnar,Gang Zhen,Longhui Zeng,Dylan M Parker,Andy L Cox,Antonio J Giraldez,Xiaolei Su,Christine Mayr,Siyuan Wang,Stefania Nicoli

Oncotarget 8:13003-13014 PubMed28099903

2017

Talin-1 interaction network promotes hepatocellular carcinoma progression.

Applications

Unspecified application

Species

Unspecified reactive species

Peng Chen,Xiaohu Zheng,Yonggang Zhou,Yechuan Xu,Lixin Zhu,Yeben Qian

Viruses 8: PubMed27775586

2016

Hepatitis B Virus Protein X Induces Degradation of Talin-1.

Applications

WB

Species

Human

Maarten A A van de Klundert,Maartje van den Biggelaar,Neeltje A Kootstra,Hans L Zaaijer

The Journal of cell biology 213:371-83 PubMed27161398

2016

Talin tension sensor reveals novel features of focal adhesion force transmission and mechanosensitivity.

Applications

WB

Species

Unspecified reactive species

Abhishek Kumar,Mingxing Ouyang,Koen Van den Dries,Ewan James McGhee,Keiichiro Tanaka,Marie D Anderson,Alexander Groisman,Benjamin T Goult,Kurt I Anderson,Martin A Schwartz

International journal of clinical and experimental 8:17748-56 PubMed26770365

2015

MicroRNA-9 is a ponderable index for the prognosis of human hepatocellular carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Jingchang Sun,Kunpeng Fang,Hang Shen,Yeben Qian

Proceedings of the National Academy of Sciences of 112:E4864-73 PubMed26283369

2015

Talin determines the nanoscale architecture of focal adhesions.

Applications

Unspecified application

Species

Unspecified reactive species

Jaron Liu,Yilin Wang,Wah Ing Goh,Honzhen Goh,Michelle A Baird,Svenja Ruehland,Shijia Teo,Neil Bate,David R Critchley,Michael W Davidson,Pakorn Kanchanawong
View all publications
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com