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Mouse Recombinant Monoclonal Talin 1 antibody. Suitable for IP, WB, IHC-P and reacts with Mouse, Rat, Human samples. Cited in 9 publications.

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Images

Western blot - Anti-Talin 1 antibody [97H6] (AB108480), expandable thumbnail
  • Immunoprecipitation - Anti-Talin 1 antibody [97H6] (AB108480), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Talin 1 antibody [97H6] (AB108480), expandable thumbnail

Publications

Key facts

Isotype
IgG1
Host species
Mouse
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPWBIHC-P
Human
Expected
Tested
Not recommended
Mouse
Tested
Tested
Tested
Rat
Expected
Tested
Not recommended

Tested
Tested

Species
Mouse
Dilution info
16.033 µg/mL
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Expected
Expected

Species
Rat, Human
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Mouse
Dilution info
0.481 µg/mL
Notes

-

Species
Rat
Dilution info
0.481 µg/mL
Notes

-

Species
Human
Dilution info
0.481 µg/mL
Notes

-

Tested
Tested

Species
Mouse
Dilution info
0.241 µg/mL
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species
Human
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Rat
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target data

Function

High molecular weight cytoskeletal protein concentrated at regions of cell-matrix and cell-cell contacts. Involved in connections of major cytoskeletal structures to the plasma membrane. With KANK1 co-organize the assembly of cortical microtubule stabilizing complexes (CMSCs) positioned to control microtubule-actin crosstalk at focal adhesions (FAs) rims.

Alternative names

Tln, Tln1, Talin-1

Recommended products

Mouse Recombinant Monoclonal Talin 1 antibody. Suitable for IP, WB, IHC-P and reacts with Mouse, Rat, Human samples. Cited in 9 publications.

Key facts

Isotype
IgG1
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
97H6
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Talin 1 (TLN1) is a cytoskeletal protein that mechanically links integrins to actin filaments in cells. It has a molecular weight of 270 kDa and is expressed in many tissues including muscle brain and kidney. Talin 1 contains a head domain and a tail domain which bind to integrins and actin respectively. By establishing these connections Talin 1 plays a role in cell adhesion spreading and migration. This protein also interacts with other adhesion-associated proteins reinforcing its structural function inside cells.

Biological function summary

Talin 1 is involved in facilitating integrin activation and focal adhesion formation. It is a component of a larger complex that includes proteins like vinculin and paxillin. These complexes are responsible for transmitting mechanical signals from the extracellular matrix into the intracellular environment initiating cellular responses like growth and differentiation. Talin 1 also modulates actin dynamics which contributes to changes in cell shape and motility. Its role in these processes highlights its significance in maintaining cellular architecture.

Pathways

Talin 1 participates in the integrin-mediated signaling pathway and the Rho GTPase signaling pathway. In the integrin pathway Talin 1 interacts with integrin beta subunits leading to integrin clustering and increased focal adhesion strength. In the Rho GTPase pathway Talin 1 influences actin cytoskeleton rearrangements through its interactions with proteins like RhoA and Rac1. These pathways are critical for processes such as cell proliferation and migration which are essential for proper tissue function and development.

Associated diseases and disorders

Talin 1 has an association with cancer and cardiovascular diseases. Changes in Talin 1 expression and localization can contribute to altered cell adhesion and motility promoting cancer cell invasion and metastasis. Talin 1 has a relationship with the protein FAK (Focal Adhesion Kinase) in cancer progression. In cardiovascular diseases Talin 1 dysregulation affects cardiomyocyte adhesion and survival and it interacts with proteins such as vinculin to sustain cardiac tissue integrity. These connections highlight Talin 1's role in disease pathology.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

3 product images

  • Western blot - Anti-Talin 1 antibody [97H6] (ab108480), expandable thumbnail

    Western blot - Anti-Talin 1 antibody [97H6] (ab108480)

    The expression profile/molecular weight observed is consistent with what has been described in the literature (PMID:22306379 and 25751747).

    Blocking/Dilution buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-Talin 1 antibody [97H6] (ab108480) at 0.481 µg/mL

    Lane 1: HUVEC (human umbilical vein endothelial cell), whole cell lysate at 10 µg

    Lane 2: HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate at 10 µg

    Lane 3: HepG2 (human hepatocellular carcinoma epithelial cell), whole cell lysate at 10 µg

    Lane 4: C6 (rat glial tumor glial cell), whole cell lysate at 10 µg

    Lane 5: NIH/3T3 (mouse embryonic fibroblast), whole cell lysate at 10 µg

    Lane 6: MEF (mouse embryonic fibroblast (immortalized)), whole cell lysate at 10 µg

    Secondary

    All lanes: Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/5000 dilution

    Predicted band size: 269 kDa

    Exposure time: 3s

  • Immunoprecipitation - Anti-Talin 1 antibody [97H6] (ab108480), expandable thumbnail

    Immunoprecipitation - Anti-Talin 1 antibody [97H6] (ab108480)

    Talin 1 was immunoprecipitated from NIH/3T3 (mouse embryonic fibroblast), whole cell lysate with ab108480 at 1/30 dilution (2μg in 0.35mg lysates) (16.033 μg/ml). Western blot was performed on the immunoprecipitate using ab108480 at 1/1000 dilution (0.481μg/ml). Mouse IgG for IP (HRP) (Anti-mouse IgG for IP (HRP) ab131368) was used at 1/1000 dilution.

    Lane 1: NIH/3T3 whole cell lysate 10μg.

    Lane 2: ab108480 IP in NIH/3T3 whole cell lysate.

    Lane 3: Mouse monoclonal IgG1 (Mouse IgG1, kappa monoclonal [MOPC-21] - isotype control ab18443) instead of ab108480 in NIH/3T3 whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 3 seconds.

    All lanes: Immunoprecipitation - Anti-Talin 1 antibody [97H6] (ab108480)

    Predicted band size: 269 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Talin 1 antibody [97H6] (ab108480), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Talin 1 antibody [97H6] (ab108480)

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling Talin 1 with ab108480 at 1/5000 dilution (0.241μg/ml) followed by ready to use Goat Anti-mouse IgG H&L (HRP polymer) (Goat Anti-Mouse IgG H&L (HRP polymer) ab214879). Cytoplasmic staining on mouse spleen. The section was incubated with ab108480 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-mouse IgG H&L (HRP polymer) (Goat Anti-Mouse IgG H&L (HRP polymer) ab214879).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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