Anti-Talin 1 antibody

• IP

Supplier Data

Immunoprecipitation - Anti-Talin 1 antibody (AB241096)

HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) prepared using NETN lysis buffer.
Lane 1 : ab241096 used for IP at 6 μg per reaction. For WB 0.4 μg/ml.
Lane 2 : Control IgG.
Chemiluminescence detection : 10 seconds.

All lanes:

Immunoprecipitation - Anti-Talin 1 antibody (ab241096)

Predicted band size: 269 kDa

false

• WB

Supplier Data

Western blot - Anti-Talin 1 antibody (AB241096)

Lysates prepared using NETN lysis buffer.

All lanes:

Western blot - Anti-Talin 1 antibody (ab241096) at 0.1 µg/mL

Lane 1:

HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg

Lane 2:

HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg

Lane 3:

Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate at 50 µg

Lane 4:

TCMK-1 (mouse kidney epithelial cell line) whole cell lysate at 50 µg

Lane 5:

NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 50 µg

Predicted band size: 269 kDa

false

Exposure time: 10s