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Mouse Monoclonal TAMRA antibody. Suitable for IP, WB and reacts with Chemical samples. Cited in 2 publications. Immunogen corresponding to Chemical / Small Molecule corresponding to TAMRA.


Images

Immunoprecipitation - Anti-TAMRA antibody [5G5] (AB171120), expandable thumbnail
  • Western blot - Anti-TAMRA antibody [5G5] (AB171120), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Mouse
Storage buffer

Preservative: 0.05% Sodium azide
Constituents: 99% PBS, 0.1% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • Chemical / Small Molecule corresponding to TAMRA.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPWB
Chemical
Tested
Tested

Tested
Tested

Species
Chemical
Dilution info
10 µg/mg of lysate
Notes

-

Tested
Tested

Species
Chemical
Dilution info
1/1000
Notes

-

Associated Products

Select an associated product type

1 product for Alternative Product

Alternative names

Recommended products

Mouse Monoclonal TAMRA antibody. Suitable for IP, WB and reacts with Chemical samples. Cited in 2 publications. Immunogen corresponding to Chemical / Small Molecule corresponding to TAMRA.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • Chemical / Small Molecule corresponding to TAMRA.
Clone number
5G5
Purification technique
Affinity purification Protein G
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

TAMRA also known as carboxytetramethylrhodamine functions as a fluorescent dye in biochemistry and molecular biology. It has a molecular weight of approximately 430 g/mol. Researchers commonly use TAMRA in various labeling applications including tagging nucleic acids and proteins. It often expresses prominently in assay systems where visualization of biological processes in real time is necessary.

Biological function summary

Scientists utilize carboxytetramethylrhodamine in tracking molecular dynamics within the cell. Despite not forming part of a native biological complex TAMRA links to other molecules for imaging purposes. Its fluorescent properties help study biomolecular interactions and cellular localization enhancing understanding of spatial and temporal characteristics of cells.

Pathways

TAMRA does not naturally integrate into specific biological pathways on its own. Instead researchers use it to investigate and map pathways such as signal transduction and nucleotide metabolism. Its interactions with macromolecules like proteins can reveal how proteins such as G-proteins affect cellular functions in pathway analyses offering insights into cellular signaling mechanisms.

Associated diseases and disorders

Scientists use TAMRA extensively to study and diagnose conditions like cancer and Alzheimer's disease. By tagging proteins involved such as beta-amyloid in Alzheimer's researchers can visualize and track pathological processes in cells. This ability enhances detection and provides data for drug targeting and therapy development.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

2 product images

  • Immunoprecipitation - Anti-TAMRA antibody [5G5] (ab171120), expandable thumbnail

    Immunoprecipitation - Anti-TAMRA antibody [5G5] (ab171120)

    Immunoprecipitation was performed on purified TAMRA-BSA. The antigen:antibody complex was formed by binding 5 μg protein with 10 μg of ab171120 or mouse IgG, whole molecule overnight at 4°C. The immune-complex was then captured on 50 μl Protein A/G Plus Agarose. Captured immune-complexes were then washes extensively and proteins eluted with sample loading dye. Samples were resolved on a SDS PAGE gel.

    Lane 1: ab171120

    Lane 2: BSA

    Lane 3: TAMRA-BSA

    Lane 4: BSA-ab171120

    Lane 5: TAMRA-BSA + mouse IgG

    Lane 6: TAMRA-BSA + ab171120

    All lanes: Immunoprecipitation - Anti-TAMRA antibody [5G5] (ab171120)

  • Western blot - Anti-TAMRA antibody [5G5] (ab171120), expandable thumbnail

    Western blot - Anti-TAMRA antibody [5G5] (ab171120)

    All lanes: Western blot - Anti-TAMRA antibody [5G5] (ab171120) at 1/1000 dilution

    Lane 1: TAMRA-BSA at 10 µg

    Lane 2: TAMRA-BSA at 5 µg

    Lane 3: TAMRA-BSA at 2 µg

    Lane 4: TAMRA-BSA at 1 µg

    Lane 5: TAMRA-BSA at 0.5 µg

    Lane 6: TAMRA-BSA

    Secondary

    All lanes: Goat anti-mouse-HRP

    Developed using the ECL technique.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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