JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB171120

Anti-TAMRA antibody [5G5]

2

(1 Review)

|

(3 Publications)

Mouse Monoclonal TAMRA antibody. Suitable for IP, WB and reacts with Chemical samples. Cited in 3 publications. Immunogen corresponding to Chemical / Small Molecule corresponding to TAMRA.
2 Images
Immunoprecipitation - Anti-TAMRA antibody [5G5] (AB171120)
  • IP

Supplier Data

Immunoprecipitation - Anti-TAMRA antibody [5G5] (AB171120)

Immunoprecipitation was performed on purified TAMRA-BSA. The antigen : antibody complex was formed by binding 5 μg protein with 10 μg of ab171120 or mouse IgG, whole molecule overnight at 4°C. The immune-complex was then captured on 50 μl Protein A/G Plus Agarose. Captured immune-complexes were then washes extensively and proteins eluted with sample loading dye. Samples were resolved on a SDS PAGE gel.

Lane 1 : ab171120

Lane 2 : BSA

Lane 3 : TAMRA-BSA

Lane 4 : BSA-ab171120

Lane 5 : TAMRA-BSA + mouse IgG

Lane 6 : TAMRA-BSA + ab171120

All lanes:

Immunoprecipitation - Anti-TAMRA antibody [5G5] (ab171120)

false

Western blot - Anti-TAMRA antibody [5G5] (AB171120)
  • WB

Supplier Data

Western blot - Anti-TAMRA antibody [5G5] (AB171120)

All lanes:

Western blot - Anti-TAMRA antibody [5G5] (ab171120) at 1/1000 dilution

Lane 1:

TAMRA-BSA at 10 µg

Lane 2:

TAMRA-BSA at 5 µg

Lane 3:

TAMRA-BSA at 2 µg

Lane 4:

TAMRA-BSA at 1 µg

Lane 5:

TAMRA-BSA at 0.5 µg

Lane 6:

TAMRA-BSA

Secondary

All lanes:

Goat anti-mouse-HRP

true

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

5G5

Isotype

IgG

Carrier free

No

Applications

IP, WB

applications

style
left-column

Alternative Products

Primary Antibodies

AB173827

Anti-TAMRA antibody [11H10]

  • 0
  • 0

Complementary Products

Primary Antibodies

AB173827

Anti-TAMRA antibody [11H10]

  • 0
  • 0
Primary Antibodies

AB52060

Anti-Cy3 / Cy5 antibody [CY96]

  • 4
  • 1
Primary Antibodies

AB52061

Anti-Cy5 antibody [CY5-15]

  • 3
  • 1
Secondary Antibodies

AB150113

Goat Anti-Mouse IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (AB150113)

  • 5
  • 15
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Chemical": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "10 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" } } }
style
left-column
style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Storage buffer
Preservative: 0.05% Sodium azide Constituents: PBS, 0.1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TAMRA also known as carboxytetramethylrhodamine functions as a fluorescent dye in biochemistry and molecular biology. It has a molecular weight of approximately 430 g/mol. Researchers commonly use TAMRA in various labeling applications including tagging nucleic acids and proteins. It often expresses prominently in assay systems where visualization of biological processes in real time is necessary.
Biological function summary

Scientists utilize carboxytetramethylrhodamine in tracking molecular dynamics within the cell. Despite not forming part of a native biological complex TAMRA links to other molecules for imaging purposes. Its fluorescent properties help study biomolecular interactions and cellular localization enhancing understanding of spatial and temporal characteristics of cells.

Pathways

TAMRA does not naturally integrate into specific biological pathways on its own. Instead researchers use it to investigate and map pathways such as signal transduction and nucleotide metabolism. Its interactions with macromolecules like proteins can reveal how proteins such as G-proteins affect cellular functions in pathway analyses offering insights into cellular signaling mechanisms.

Scientists use TAMRA extensively to study and diagnose conditions like cancer and Alzheimer's disease. By tagging proteins involved such as beta-amyloid in Alzheimer's researchers can visualize and track pathological processes in cells. This ability enhances detection and provides data for drug targeting and therapy development.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

See full target information TAMRA
style
left-column

Publications (3)

Recent publications for all applications. Explore the full list and refine your search

British journal of cancer 133:756-770 PubMed40646287

2025

Targeting ribosomes reprograms the tumour microenvironment and augments cancer immunotherapy.

Applications

Unspecified application

Species

Unspecified reactive species

Kaisa Cui,Bingxin Liu,Liang Gong,Quan Wan,Hong Tang,Zhicheng Gong,Renhui Shen,Chao Wang,Qiang Zhang,Qilin Li,Yizhun Zhu,Youming Zhang,Xiaojie Lu

Frontiers in oncology 11:681366 PubMed34136404

2021

N-Myristoylation by NMT1 Is POTEE-Dependent to Stimulate Liver Tumorigenesis Differentially Regulating Ubiquitination of Targets.

Applications

Unspecified application

Species

Unspecified reactive species

Guoqing Zhu,Feng Wang,Haojie Li,Xiao Zhang,Qi Wu,Ya Liu,Mingping Qian,Susu Guo,Yueyue Yang,Xiangfei Xue,Fenyong Sun,Yongxia Qiao,Qiuhui Pan

Developmental cell 53:691-705.e7 PubMed32533921

2020

Prenylation of Axonally Translated Rac1 Controls NGF-Dependent Axon Growth.

Applications

Unspecified application

Species

Unspecified reactive species

Emily Scott-Solomon,Rejji Kuruvilla
View all publications
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com