AB47632

Anti-TANK/TRAF2 antibody

Name: Anti-TANK/TRAF2 antibody (ab47632) | Abcam
Brand: Abcam Limited
SKU: AB47632

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(2 Publications)

Rabbit Polyclonal TANK/TRAF2 antibody. Suitable for WB and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human TANK.

View Alternative Names

ITRAF, TRAF2, TANK, TRAF family member-associated NF-kappa-B activator, TRAF-interacting protein, I-TRAF

2 Images

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Western blot - Anti-TANK/TRAF2 antibody (AB47632)

Western Blot of human kidney lysate labeling TANK/TRAF2 with Anti-TANK/TRAF2 antibody (ab47632) at (1) 1 and (2) 2μg/ml.

All lanes:

Western blot - Anti-TANK/TRAF2 antibody (ab47632)

Predicted band size: 48 kDa

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Western blot - Anti-TANK/TRAF2 antibody (AB47632)

Lane 1:

Western blot - Anti-TANK/TRAF2 antibody (ab47632) at 0.5 µg/mL

Lane 2:

Western blot - Anti-TANK/TRAF2 antibody (ab47632) at 1 µg/mL

Lane 3:

Western blot - Anti-TANK/TRAF2 antibody (ab47632) at 2 µg/mL

All lanes:

Daudi Cell Lysate at 15 µg

Predicted band size: 48 kDa

Observed band size: 55 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

Synthetic Peptide within Human TANK. The exact immunogen used to generate this antibody is proprietary information.

Q92844

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-2 µg/mL", "WB-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TANK (TRAF family member-associated NF-kappa-B activator) and TRAF2 (TNF receptor-associated factor 2) are proteins involved in the regulation of immune responses. TANK also known as I-TRAF is a protein with a mass of approximately 49 kDa. TRAF2 has a mass of approximately 56 kDa. These proteins express in various immune cells including T and B lymphocytes. TANK interacts with TRAF2 to mediate downstream signaling pathways essential for immunity.

Biological function summary

The TANK/TRAF2 complex plays a role in modulating the NF-kappa-B signaling pathway which is important for inflammation and immune responses. This complex also contributes to cellular responses to stress and infection. TANK acts as an adaptor protein that interacts with TRAF2 to relay signals from upstream receptors ensuring proper immune function. Additionally TANK inhibits excessive activation of NF-kappa-B maintaining a balanced immune response.

Pathways

As it relates to regulating immune functions TANK/TRAF2 is pivotal in the NF-kappa-B and MAPK signaling pathways. In the NF-kappa-B pathway TRAF2 helps mediate the activation of downstream kinases which are critical for the translocation of NF-kappa-B to the nucleus. TANK works alongside other signaling molecules like TRAF3 and TRAF6 integrating signals that lead to gene transcription involved in immune response and cell survival.

The dysfunction of TANK/TRAF2 differs from normal regulatory controls it links to auto-inflammatory diseases and certain cancers. In autoimmune disorders such as systemic lupus erythematosus aberrant NF-kappa-B activity possibly influenced by TANK/TRAF2 leads to uncontrolled immune activation. In cancer particularly lymphomas altered TANK/TRAF2 signaling correlates with the unchecked proliferation of cells. The protein's interplay with receptor-interacting protein 1 (RIP1) further emphasizes its role as disruptions in this interaction are seen in pathological states.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Adapter protein involved in I-kappa-B-kinase (IKK) regulation which constitutively binds TBK1 and IKBKE playing a role in antiviral innate immunity. Acts as a regulator of TRAF function by maintaining them in a latent state. Blocks TRAF2 binding to LMP1 and inhibits LMP1-mediated NF-kappa-B activation. Negatively regulates NF-kappaB signaling and cell survival upon DNA damage (PubMed : 25861989). Plays a role as an adapter to assemble ZC3H12A, USP10 in a deubiquitination complex which plays a negative feedback response to attenuate NF-kappaB activation through the deubiquitination of IKBKG or TRAF6 in response to interleukin-1-beta (IL1B) stimulation or upon DNA damage (PubMed : 25861989). Promotes UBP10-induced deubiquitination of TRAF6 in response to DNA damage (PubMed : 25861989). May control negatively TRAF2-mediated NF-kappa-B activation signaled by CD40, TNFR1 and TNFR2.

See full target information TANK

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Cell reports 42:111949 PubMed36640307

2023

Rabies virus P protein binds to TBK1 and interferes with the formation of innate immunity-related liquid condensates.

Applications

Unspecified application

Species

Unspecified reactive species

Nathalie Scrima,Romain Le Bars,Quentin Nevers,Damien Glon,Guillaume Chevreux,Ahmet Civas,Danielle Blondel,Cécile Lagaudrière-Gesbert,Yves Gaudin

Cell reports 32:108146 PubMed32937131

2020

Extensive SUMO Modification of Repressive Chromatin Factors Distinguishes Pluripotent from Somatic Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Ilan Theurillat,Ivo A Hendriks,Jack-Christophe Cossec,Alexandra Andrieux,Michael L Nielsen,Anne Dejean

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-TANK/TRAF2 antibody

Western blot - Anti-TANK/TRAF2 antibody (AB47632)

Western blot - Anti-TANK/TRAF2 antibody (AB47632)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

Product promise