AB124841

Anti-TAOK2 + TAOK1 + TAOK3 (phospho S177 + S181 + S181) antibody [EPR4883]

RabMAb
Recombinant
What is this?

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(2 Publications)

Rabbit Recombinant Monoclonal TAOK2 phospho S181 antibody. Suitable for WB and reacts with Mouse, Rat, Human samples. Cited in 2 publications.

View Alternative Names

KIAA0881, MAP3K17, PSK, PSK1, UNQ2971/PRO7431, TAOK2, Serine/threonine-protein kinase TAO2, Kinase from chicken homolog C, Prostate-derived sterile 20-like kinase 1, Thousand and one amino acid protein kinase 2, hKFC-C, PSK-1, Prostate-derived STE20-like kinase 1

3 Images

Lab

Western blot - Anti-TAOK2 + TAOK1 + TAOK3 (phospho S177 + S181 + S181) antibody [EPR4883] (AB124841)

We are unsure about the nature of other bands. Bands at 110 to 140.

All lanes:

Western blot - Anti-TAOK2 + TAOK1 + TAOK3 (phospho S177 + S181 + S181) antibody [EPR4883] (ab124841) at 1/10000 dilution

Lane 1:

HEK-293 (Human embryonic kidney epithelial cell) whole cell lysate at 15 µg

Lane 2:

HEK-293 (Human embryonic kidney epithelial cell) whole cell lysate, then the membrane treated with Lambda Phosphatase at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 138 kDa

Observed band size: 110-140 kDa

false

Lab

Western blot - Anti-TAOK2 + TAOK1 + TAOK3 (phospho S177 + S181 + S181) antibody [EPR4883] (AB124841)

We are unsure about the nature of the extra bands. Bands at 110 to 140.

All lanes:

Western blot - Anti-TAOK2 + TAOK1 + TAOK3 (phospho S177 + S181 + S181) antibody [EPR4883] (ab124841) at 1/10000 dilution

Lane 1:

Mouse brain lysate at 15 µg

Lane 2:

Mouse brain lysate, then the membrane treated with Lambda Phosphatase at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 138 kDa

Observed band size: 110-140 kDa

false

Lab

Western blot - Anti-TAOK2 + TAOK1 + TAOK3 (phospho S177 + S181 + S181) antibody [EPR4883] (AB124841)

We are unsure about the nature of the extra bands. Bands at 110 to 140.

All lanes:

Western blot - Anti-TAOK2 + TAOK1 + TAOK3 (phospho S177 + S181 + S181) antibody [EPR4883] (ab124841) at 1/10000 dilution

Lane 1:

Rat brain lysate at 15 µg

Lane 2:

Rat brain lysate, then the membrane treated with Lambda Phosphatase at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 138 kDa

Observed band size: 110-140 kDa

false

Carrier free

Anti-TAOK2 + TAOK1 + TAOK3 (phospho S177 + S181 + S181) antibody [EPR4883] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR4883

Isotype

IgG

Carrier free

Reacts with

Human, Mouse, Rat

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

ab124841 recognizes Human TAOK1 when phosphorylated at Serine 181, TAOK2 when phosphorylated at Serine 181 and TAOK3 when phosphorylated at Serine 177.

ab124841 cross reactivates with MEK1 due to high immunogen homology.

The antibody is detecting TAOK2 + TAOK1 + TAOK3 at around 138 kDa and MAP2K1 at around 44kDa.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "1/1000 - 1/8000", "IHCP-species-notes": "Antigen retrieval is recommended.1:1000 (Hu) / 1:8000 (Ms & Rt)For unpurified use at 1:250 - 1:500", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "" }, "Mouse": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "1/1000 - 1/8000", "IHCP-species-notes": "Antigen retrieval is recommended.1:1000 (Hu) / 1:8000 (Ms & Rt)For unpurified use at 1:250 - 1:500", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "1/1000 - 1/8000", "IHCP-species-notes": "Antigen retrieval is recommended.1:1000 (Hu) / 1:8000 (Ms & Rt)For unpurified use at 1:250 - 1:500", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TAOK2 TAOK1 and TAOK3 are members of the TAO (thousand and one amino acid) kinase family which are serine/threonine protein kinases. These kinases are known alternative names such as KINASE ORCCAN and MAP3K16. They operate through the phosphorylation of substrates to relay signals within cells a vital function in maintaining cellular processes. TAOK family proteins have varying molecular masses with TAOK2 weighing around 130 kDa. These kinases exhibit broad expression in the human body but are notably present in the brain suggesting a significant role in neurological functions.

Biological function summary

TAOK family kinases participate in the regulation of cell cycle apoptosis and stress response. They interact with various signaling complexes serving important roles in maintaining cellular homeostasis. Their ability to influence cytoskeletal dynamics further highlights their involvement in controlling cell morphology and mobility. Their activity is therefore important in ensuring that cells respond appropriately to internal and external stimuli promoting overall cellular health and function.

Pathways

The TAOK family kinases engage actively in the MAPK signaling cascade and JNK signaling pathway. Their engagement in these pathways links them to other proteins such as MAPK14 and JNK3 which play pivotal roles in transmitting stress signals thereby enabling adaptation and survival mechanisms within cells. These signaling routes allow cells to adjust to changes in the environment by regulating gene expression and cellular responses.

Altered TAOK kinase activity connects to neurodevelopmental disorders such as autism spectrum disorder and neurodegenerative conditions like Alzheimer's disease. Disruptions in TAOK signaling may affect proteins such as NRXN1 and APP known for their roles in synaptic function and neuronal integrity respectively. These connections emphasize the importance of TAOK kinases in neural tissue and highlight potential avenues for therapeutic interventions targeting these kinases in relevant neurological diseases.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Serine/threonine-protein kinase involved in different processes such as membrane blebbing and apoptotic bodies formation DNA damage response and MAPK14/p38 MAPK stress-activated MAPK cascade. Phosphorylates itself, MBP, activated MAPK8, MAP2K3, MAP2K6 and tubulins. Activates the MAPK14/p38 MAPK signaling pathway through the specific activation and phosphorylation of the upstream MAP2K3 and MAP2K6 kinases. In response to DNA damage, involved in the G2/M transition DNA damage checkpoint by activating the p38/MAPK14 stress-activated MAPK cascade, probably by mediating phosphorylation of upstream MAP2K3 and MAP2K6 kinases. Isoform 1, but not isoform 2, plays a role in apoptotic morphological changes, including cell contraction, membrane blebbing and apoptotic bodies formation. This function, which requires the activation of MAPK8/JNK and nuclear localization of C-terminally truncated isoform 1, may be linked to the mitochondrial CASP9-associated death pathway. Isoform 1 binds to microtubules and affects their organization and stability independently of its kinase activity. Prevents MAP3K7-mediated activation of CHUK, and thus NF-kappa-B activation, but not that of MAPK8/JNK. May play a role in the osmotic stress-MAPK8 pathway. Isoform 2, but not isoform 1, is required for PCDH8 endocytosis. Following homophilic interactions between PCDH8 extracellular domains, isoform 2 phosphorylates and activates MAPK14/p38 MAPK which in turn phosphorylates isoform 2. This process leads to PCDH8 endocytosis and CDH2 cointernalization. Both isoforms are involved in MAPK14 phosphorylation.

See full target information TAOK2 phospho S181

Additional targets

TAOK1 phospho S181,TAOK3 phospho S177

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

eLife 10: PubMed33973518

2021

Limited inhibition of multiple nodes in a driver network blocks metastasis.

Applications

Unspecified application

Species

Unspecified reactive species

Ali Ekrem Yesilkanal,Dongbo Yang,Andrea Valdespino,Payal Tiwari,Alan U Sabino,Long Chi Nguyen,Jiyoung Lee,Xiao-He Xie,Siqi Sun,Christopher Dann,Lydia Robinson-Mailman,Ethan Steinberg,Timothy Stuhlmiller,Casey Frankenberger,Elizabeth Goldsmith,Gary L Johnson,Alexandre F Ramos,Marsha R Rosner

Frontiers in immunology 9:687 PubMed29696016

2018

Gα Signaling Promotes Marginal Zone B Cell Development by Enabling Transitional B Cell ADAM10 Expression.

Applications

Unspecified application

Species

Unspecified reactive species

Il-Young Hwang,Cedric Boularan,Kathleen Harrison,John H Kehrl

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-TAOK2 + TAOK1 + TAOK3 (phospho S177 + S181 + S181) antibody [EPR4883]

Western blot - Anti-TAOK2 + TAOK1 + TAOK3 (phospho S177 + S181 + S181) antibody [EPR4883] (AB124841)

Western blot - Anti-TAOK2 + TAOK1 + TAOK3 (phospho S177 + S181 + S181) antibody [EPR4883] (AB124841)

Western blot - Anti-TAOK2 + TAOK1 + TAOK3 (phospho S177 + S181 + S181) antibody [EPR4883] (AB124841)

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Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Additional targets

Publications (2)

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