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AB248030

Anti-TAOK2 + TAOK1 + TAOK3 (phospho S177 + S181 + S181) antibody [EPR4883] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal TAOK1 phospho S181 antibody. Carrier free. Suitable for WB and reacts with Mouse, Rat, Human samples. Cited in 1 publication.

View Alternative Names

KIAA1361, MAP3K16, MARKK, TAOK1, Serine/threonine-protein kinase TAO1, Kinase from chicken homolog B, MARK Kinase, Prostate-derived sterile 20-like kinase 2, Thousand and one amino acid protein kinase 1, hKFC-B, PSK-2, PSK2, Prostate-derived STE20-like kinase 2, hTAOK1

3 Images
Western blot - Anti-TAOK2 + TAOK1 + TAOK3 (phospho S177 + S181 + S181) antibody [EPR4883] - BSA and Azide free (AB248030)
  • WB

Lab

Western blot - Anti-TAOK2 + TAOK1 + TAOK3 (phospho S177 + S181 + S181) antibody [EPR4883] - BSA and Azide free (AB248030)

This data was developed using ab124841 the same antibody clone in a different buffer formulation.

We are unsure about the nature of the extra bands. Bands at 110 to 140.

All lanes:

Western blot - Anti-TAOK2 + TAOK1 + TAOK3 (phospho S177 + S181 + S181) antibody [EPR4883] (<a href='/en-us/products/primary-antibodies/taok2-taok1-taok3-phospho-s177-s181-s181-antibody-epr4883-ab124841'>ab124841</a>) at 1/10000 dilution

Lane 1:

Mouse brain lysate at 15 µg

Lane 2:

Mouse brain lysate, then the membrane treated with Lambda Phosphatase at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 138 kDa

Observed band size: 110-140 kDa

false

Western blot - Anti-TAOK2 + TAOK1 + TAOK3 (phospho S177 + S181 + S181) antibody [EPR4883] - BSA and Azide free (AB248030)
  • WB

Lab

Western blot - Anti-TAOK2 + TAOK1 + TAOK3 (phospho S177 + S181 + S181) antibody [EPR4883] - BSA and Azide free (AB248030)

This data was developed using ab124841 the same antibody clone in a different buffer formulation.

We are unsure about the nature of the extra bands. Bands at 110 to 140.

All lanes:

Western blot - Anti-TAOK2 + TAOK1 + TAOK3 (phospho S177 + S181 + S181) antibody [EPR4883] (<a href='/en-us/products/primary-antibodies/taok2-taok1-taok3-phospho-s177-s181-s181-antibody-epr4883-ab124841'>ab124841</a>) at 1/10000 dilution

Lane 1:

Rat brain lysate at 15 µg

Lane 2:

Rat brain lysate, then the membrane treated with Lambda Phosphatase at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 138 kDa

Observed band size: 110-140 kDa

false

Western blot - Anti-TAOK2 + TAOK1 + TAOK3 (phospho S177 + S181 + S181) antibody [EPR4883] - BSA and Azide free (AB248030)
  • WB

Lab

Western blot - Anti-TAOK2 + TAOK1 + TAOK3 (phospho S177 + S181 + S181) antibody [EPR4883] - BSA and Azide free (AB248030)

This data was developed using ab124841 the same antibody clone in a different buffer formulation.

We are unsure about the nature of other bands. Bands at 110 to 140.

All lanes:

Western blot - Anti-TAOK2 + TAOK1 + TAOK3 (phospho S177 + S181 + S181) antibody [EPR4883] (<a href='/en-us/products/primary-antibodies/taok2-taok1-taok3-phospho-s177-s181-s181-antibody-epr4883-ab124841'>ab124841</a>) at 1/10000 dilution

Lane 1:

HEK-293 (Human embryonic kidney epithelial cell) whole cell lysate at 15 µg

Lane 2:

HEK-293 (Human embryonic kidney epithelial cell) whole cell lysate, then the membrane treated with Lambda Phosphatase at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 138 kDa

Observed band size: 110-140 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR4883

Isotype

IgG

Carrier free

Yes

Reacts with

Human, Mouse, Rat

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

This antibody recognizes Human TAOK1 when phosphorylated at Serine 181, TAOK2 when phosphorylated at Serine 181 and TAOK3 when phosphorylated at Serine 177.

ab248030 cross reactivates with MEK1 due to high immunogen homology.

The antibody is detecting TAOK2 + TAOK1 + TAOK3 at around 138 kDa and MAP2K1 at around 44kDa.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p>Antigen retrieval is recommended.</p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p>Antigen retrieval is recommended.</p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Rat": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p>Antigen retrieval is recommended.</p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }
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Product details

ab248030 is the carrier-free version of ab124841.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TAOK2 TAOK1 and TAOK3 are members of the TAO (thousand and one amino acid) kinase family which are serine/threonine protein kinases. These kinases are known alternative names such as KINASE ORCCAN and MAP3K16. They operate through the phosphorylation of substrates to relay signals within cells a vital function in maintaining cellular processes. TAOK family proteins have varying molecular masses with TAOK2 weighing around 130 kDa. These kinases exhibit broad expression in the human body but are notably present in the brain suggesting a significant role in neurological functions.
Biological function summary

TAOK family kinases participate in the regulation of cell cycle apoptosis and stress response. They interact with various signaling complexes serving important roles in maintaining cellular homeostasis. Their ability to influence cytoskeletal dynamics further highlights their involvement in controlling cell morphology and mobility. Their activity is therefore important in ensuring that cells respond appropriately to internal and external stimuli promoting overall cellular health and function.

Pathways

The TAOK family kinases engage actively in the MAPK signaling cascade and JNK signaling pathway. Their engagement in these pathways links them to other proteins such as MAPK14 and JNK3 which play pivotal roles in transmitting stress signals thereby enabling adaptation and survival mechanisms within cells. These signaling routes allow cells to adjust to changes in the environment by regulating gene expression and cellular responses.

Altered TAOK kinase activity connects to neurodevelopmental disorders such as autism spectrum disorder and neurodegenerative conditions like Alzheimer's disease. Disruptions in TAOK signaling may affect proteins such as NRXN1 and APP known for their roles in synaptic function and neuronal integrity respectively. These connections emphasize the importance of TAOK kinases in neural tissue and highlight potential avenues for therapeutic interventions targeting these kinases in relevant neurological diseases.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Serine/threonine-protein kinase involved in various processes such as p38/MAPK14 stress-activated MAPK cascade, DNA damage response and regulation of cytoskeleton stability. Phosphorylates MAP2K3, MAP2K6 and MARK2. Acts as an activator of the p38/MAPK14 stress-activated MAPK cascade by mediating phosphorylation and subsequent activation of the upstream MAP2K3 and MAP2K6 kinases. Involved in G-protein coupled receptor signaling to p38/MAPK14. In response to DNA damage, involved in the G2/M transition DNA damage checkpoint by activating the p38/MAPK14 stress-activated MAPK cascade, probably by mediating phosphorylation of MAP2K3 and MAP2K6. Acts as a regulator of cytoskeleton stability by phosphorylating 'Thr-208' of MARK2, leading to activate MARK2 kinase activity and subsequent phosphorylation and detachment of MAPT/TAU from microtubules. Also acts as a regulator of apoptosis : regulates apoptotic morphological changes, including cell contraction, membrane blebbing and apoptotic bodies formation via activation of the MAPK8/JNK cascade. Plays an essential role in the regulation of neuronal development in the central nervous system (PubMed : 33565190). Also plays a role in the regulation of neuronal migration to the cortical plate (By similarity).
See full target information TAOK1 phospho S181

Additional targets

,TAOK1 phospho S181,
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of virology 97:e0190722 PubMed36946735

2023

Characterization of the Impact of Merkel Cell Polyomavirus-Induced Interferon Signaling on Viral Infection.

Applications

Unspecified application

Species

Unspecified reactive species

Ranran Wang,June F Yang,Taylor E Senay,Wei Liu,Jianxin You
View all publications
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Product promise

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