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Mouse Monoclonal TAP tag antibody. C-terminal. Suitable for WB and reacts with Tag samples.

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Images

Western blot - Anti-TAP tag antibody [22F12-1E3] - C-terminal (AB185976), expandable thumbnail

Key facts

Isotype

IgG1

Host species

Mouse

Storage buffer

Preservative: 0.05% Sodium azide
Constituents: 69% PBS, 30% Glycerol (glycerin, glycerine), 0.1% BSA

Form

Liquid

Clonality

Monoclonal

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WB
Tag
Tested

Tested
Tested

Species

Tag

Dilution info

1/1000 - 1/2000

Notes

-

Recommended products

Mouse Monoclonal TAP tag antibody. C-terminal. Suitable for WB and reacts with Tag samples.

Key facts

Isotype

IgG1

Form

Liquid

Clonality

Monoclonal

Clone number

22F12-1E3

Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

TAP adds about 20 kDa to the size of the protein.

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

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Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

The TAP tag also known as the Tandem Affinity Purification tag is a biotechnological tool used for studying protein interactions and functions. It is not a natural protein but rather an engineered tag that researchers attach to target proteins for purification and analysis purposes. The TAP tag comprises two protein domains typically Protein A and calmodulin-binding peptide connected by a TEV protease cleavage site and has a total mass of approximately 21 kDa. Researchers frequently express TAP-tagged proteins in yeast cells or mammalian systems to facilitate the study of complex protein networks in these models.

Biological function summary

TAP tags allow for the isolation and analysis of protein complexes from cells. This occurs through a multi-step purification process leveraging the tag's affinity for specific binding partners. Using TAP tags enhances the identification and study of interacting proteins and complexes improving our understanding of biological processes. The TAP tag system is often combined with mass spectrometry to identify additional complex members enhancing the resolution of protein interaction networks.

Pathways

TAP tags enable the investigation of proteins involved in key pathways by isolating their associated complexes. Notable pathways where TAP tags have facilitated research include signal transduction and cell cycle regulation. Within these pathways TAP tags help identify interactions with proteins like kinases and phosphatases providing insights into pathway dynamics and control mechanisms. The use of TAP tagging in pathway analysis can also reveal cross-talk between pathways by uncovering shared or sequentially interacting proteins.

Associated diseases and disorders

TAP tags have been instrumental in revealing interactions relevant to cancer and neurodegenerative diseases. Researchers have used TAP tags to study molecules involved in tumor progression and metastasis shedding light on proteins like p53 and its network of interactions. Similarly in neurodegenerative conditions such as Alzheimer's disease TAP tags facilitate the identification of protein complexes that include beta-amyloid precursor protein (APP) and associated interactors offering insights into pathogenic mechanisms. These studies can help identify new biomarkers or therapeutic targets by elucidating the complex protein interactions modulating disease processes.

Product promise

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In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
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1 product image

  • Western blot - Anti-TAP tag antibody [22F12-1E3] - C-terminal (ab185976), expandable thumbnail

    Western blot - Anti-TAP tag antibody [22F12-1E3] - C-terminal (ab185976)

    All lanes: Western blot - Anti-TAP tag antibody [22F12-1E3] - C-terminal (ab185976) at 1/1000 dilution

    Lane 1: TAP-tag containing control protein at 20 µL

    Lane 2: TAP-tag containing control protein at 10 µL

    Lane 3: TAP-tag containing control protein at 5 µL

    Secondary

    All lanes: goat anti-mouse IgG-HRP at 1/15000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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