Mouse Monoclonal TAP tag antibody. C-terminal. Suitable for WB and reacts with Tag samples.
IgG1
Mouse
Preservative: 0.05% Sodium azide
Constituents: 69% PBS, 30% Glycerol (glycerin, glycerine), 0.1% BSA
Liquid
Monoclonal
WB | |
---|---|
Tag | Tested |
Species | Dilution info | Notes |
---|---|---|
Species Tag | Dilution info 1/1000 - 1/2000 | Notes - |
Mouse Monoclonal TAP tag antibody. C-terminal. Suitable for WB and reacts with Tag samples.
IgG1
Mouse
Preservative: 0.05% Sodium azide
Constituents: 69% PBS, 30% Glycerol (glycerin, glycerine), 0.1% BSA
Liquid
Monoclonal
22F12-1E3
Affinity purification Protein A
Blue Ice
1-2 weeks
+4°C
-20°C
Upon delivery aliquot
Avoid freeze / thaw cycle
TAP adds about 20 kDa to the size of the protein.
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This supplementary information is collated from multiple sources and compiled automatically.
The TAP tag also known as the Tandem Affinity Purification tag is a biotechnological tool used for studying protein interactions and functions. It is not a natural protein but rather an engineered tag that researchers attach to target proteins for purification and analysis purposes. The TAP tag comprises two protein domains typically Protein A and calmodulin-binding peptide connected by a TEV protease cleavage site and has a total mass of approximately 21 kDa. Researchers frequently express TAP-tagged proteins in yeast cells or mammalian systems to facilitate the study of complex protein networks in these models.
TAP tags allow for the isolation and analysis of protein complexes from cells. This occurs through a multi-step purification process leveraging the tag's affinity for specific binding partners. Using TAP tags enhances the identification and study of interacting proteins and complexes improving our understanding of biological processes. The TAP tag system is often combined with mass spectrometry to identify additional complex members enhancing the resolution of protein interaction networks.
TAP tags enable the investigation of proteins involved in key pathways by isolating their associated complexes. Notable pathways where TAP tags have facilitated research include signal transduction and cell cycle regulation. Within these pathways TAP tags help identify interactions with proteins like kinases and phosphatases providing insights into pathway dynamics and control mechanisms. The use of TAP tagging in pathway analysis can also reveal cross-talk between pathways by uncovering shared or sequentially interacting proteins.
TAP tags have been instrumental in revealing interactions relevant to cancer and neurodegenerative diseases. Researchers have used TAP tags to study molecules involved in tumor progression and metastasis shedding light on proteins like p53 and its network of interactions. Similarly in neurodegenerative conditions such as Alzheimer's disease TAP tags facilitate the identification of protein complexes that include beta-amyloid precursor protein (APP) and associated interactors offering insights into pathogenic mechanisms. These studies can help identify new biomarkers or therapeutic targets by elucidating the complex protein interactions modulating disease processes.
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This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
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Terms & Conditions.
All lanes: Western blot - Anti-TAP tag antibody [22F12-1E3] - C-terminal (ab185976) at 1/1000 dilution
Lane 1: TAP-tag containing control protein at 20 µL
Lane 2: TAP-tag containing control protein at 10 µL
Lane 3: TAP-tag containing control protein at 5 µL
All lanes: goat anti-mouse IgG-HRP at 1/15000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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