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Rabbit Recombinant Monoclonal TAP1 antibody. Carrier free. Suitable for Flow Cyt (Intra), WB, ICC/IF, IP, Dot and reacts with Human, Recombinant fragment - Human samples.

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Images

Western blot - Anti-TAP1 antibody [EPR26236-57] - BSA and Azide free (AB314746), expandable thumbnail
  • Immunoprecipitation - Anti-TAP1 antibody [EPR26236-57] - BSA and Azide free (AB314746), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-TAP1 antibody [EPR26236-57] - BSA and Azide free (AB314746), expandable thumbnail
  • Dot Blot - Anti-TAP1 antibody [EPR26236-57] - BSA and Azide free (AB314746), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-TAP1 antibody [EPR26236-57] - BSA and Azide free (AB314746), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Constituents: 100% PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
Flow Cyt (Intra)WBICC/IFIPDotIHC-P
Human
Tested
Tested
Tested
Tested
Expected
Not recommended
Mouse
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Rat
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Recombinant fragment - Human
Not recommended
Not recommended
Not recommended
Not recommended
Tested
Not recommended

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat, Recombinant fragment - Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat, Recombinant fragment - Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat, Recombinant fragment - Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat, Recombinant fragment - Human
Dilution info
-
Notes

-

Tested
Tested

Species
Recombinant fragment - Human
Dilution info
-
Notes

-

Expected
Expected

Species
Human
Dilution info
Use at an assay dependent concentration.
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat, Human, Recombinant fragment - Human
Dilution info
-
Notes

-

Associated Products

Select an associated product type

1 product for Alternative Version

Target data

Function

ABC transporter associated with antigen processing. In complex with TAP2 mediates unidirectional translocation of peptide antigens from cytosol to endoplasmic reticulum (ER) for loading onto MHC class I (MHCI) molecules (PubMed:25377891, PubMed:25656091). Uses the chemical energy of ATP to export peptides against the concentration gradient (PubMed:25377891). During the transport cycle alternates between 'inward-facing' state with peptide binding site facing the cytosol to 'outward-facing' state with peptide binding site facing the ER lumen. Peptide antigen binding to ATP-loaded TAP1-TAP2 induces a switch to hydrolysis-competent 'outward-facing' conformation ready for peptide loading onto nascent MHCI molecules. Subsequently ATP hydrolysis resets the transporter to the 'inward facing' state for a new cycle (PubMed:11274390, PubMed:25377891, PubMed:25656091). Typically transports intracellular peptide antigens of 8 to 13 amino acids that arise from cytosolic proteolysis via IFNG-induced immunoproteasome. Binds peptides with free N- and C-termini, the first three and the C-terminal residues being critical. Preferentially selects peptides having a highly hydrophobic residue at position 3 and hydrophobic or charged residues at the C-terminal anchor. Proline at position 2 has the most destabilizing effect (PubMed:11274390, PubMed:7500034, PubMed:9256420). As a component of the peptide loading complex (PLC), acts as a molecular scaffold essential for peptide-MHCI assembly and antigen presentation (PubMed:1538751, PubMed:25377891, PubMed:26611325).

Alternative names

ABCB2, PSF1, RING4, Y3, TAP1, Antigen peptide transporter 1, APT1, ATP-binding cassette sub-family B member 2, Peptide supply factor 1, Peptide transporter PSF1, Peptide transporter TAP1, Peptide transporter involved in antigen processing 1, Really interesting new gene 4 protein, PSF-1

Recommended products

Rabbit Recombinant Monoclonal TAP1 antibody. Carrier free. Suitable for Flow Cyt (Intra), WB, ICC/IF, IP, Dot and reacts with Human, Recombinant fragment - Human samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
EPR26236-57
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C

Notes

ab314746 is the carrier-free version of Anti-TAP1 antibody [EPR26236-57] ab314745.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

The transporter associated with antigen processing 1 (TAP1) also known as ABCB2 is an ATP-binding cassette transporter with an important role in cellular immunity. TAP1 functions mechanically by transporting peptides from the cytosol into the endoplasmic reticulum. These peptides are typically 8-16 amino acids long and are processed for MHC class I molecules presentation. TAP1 has a molecular mass of approximately 75 kDa. Expression of TAP1 occurs primarily in cells of the immune system including lymphocytes and dendritic cells.

Biological function summary

TAP1 participates in the immune system by facilitating the presentation of peptide antigens to T cells. It forms a complex with TAP2 to transport antigenic peptides necessary for the proper functioning of the MHC class I pathway. This complex is essential for the immune system's ability to detect and respond to pathogens. Proper functioning of TAP1 ensures that peptides presented on MHC class I molecules are of endogenous origin allowing T cells to monitor cellular health and detect infected or transformed cells.

Pathways

TAP1 plays a role in the antigen processing and presentation pathway which is critical for adaptive immunity. This pathway involves specific steps in peptide processing where TAP1 along with TAP2 is essential for translocating peptides for binding to MHC class I complexes. Other related proteins in this pathway include calreticulin and tapasin which assist in peptide loading onto MHC class I molecules. Additionally TAP1 is involved in regulating cytosolic proteins' turnover through its role in transporting peptides that result from degraded proteins.

Associated diseases and disorders

TAP1 dysfunction or variants can lead to immune system-related diseases such as Bare Lymphocyte Syndrome (BLS) a condition characterized by the lack of MHC class I molecule expression. This results in compromised immune surveillance and increased susceptibility to infections. TAP1 is also linked to autoimmune diseases where abnormal peptide presentation may occur. It is associated with proteins like HLA molecules which are often implicated in autoimmune pathogenesis. Analyzing TAP1 expression and function provides insight into these disorders' underlying mechanisms.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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