Anti-TAP2 antibody

• ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-TAP2 antibody (AB180611)

Immunocytochemistry/Immunofluorescence analysis of U2OS cells using ab180611. Blue DAPI for nuclear staining.

• WB

Lab

Western blot - Anti-TAP2 antibody (AB180611)

False colour image of Western blot : Anti-TAP2 antibody staining at 1/500 dilution, shown in green; Mouse anti-GAPDH antibody [6C5] (ab8245) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab180611 was shown to bind specifically to TAP2. A band was observed at 75 kDa in wild-type HeLa cell lysates with no signal observed at this size in Tap2 knockout cell line ab265426 (knockout cell lysate ab258712). To generate this image, wild-type and Tap2 knockout HeLa cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween^® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed (ab216776) at 1/20000 dilution.

All lanes:

Western blot - Anti-TAP2 antibody (ab180611) at 1/500 dilution

Lane 1:

Wild-type HeLa cell lysate at 20 µg

Lane 2:

Tap2 knockout HeLa cell lysate at 20 µg

Lane 2:

Western blot - Human TAP2 knockout HeLa cell line (<a href='/en-us/products/cell-lines/human-tap2-knockout-hela-cell-line-ab265426'>ab265426</a>)

Predicted band size: 76 kDa

Observed band size: 75 kDa

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• WB

Lab

Western blot - Anti-TAP2 antibody (AB180611)

False colour image of Western blot : Anti-TAP2 antibody staining at 1/500 dilution, shown in green; Mouse anti-GAPDH antibody [6C5] (ab8245) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab180611 was shown to bind specifically to TAP2. A band was observed at 70 kDa in treated wild-type A431 cell lysates with no signal observed at this size in Tap2 knockout cell line ab269617 (knockout cell lysate ab272427). To generate this image, wild-type and Tap2 knockout A431 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.

All lanes:

Western blot - Anti-TAP2 antibody (ab180611) at 1/500 dilution

Lane 1:

Wild-type A431 Treated IFNgamma (10 ng/mL, 16 h) cell lysate at 20 µg

Lane 2:

Tap2 knockout A431 Treated IFNgamma (10 ng/mL, 16 h) cell lysate at 20 µg

Lane 3:

Wild-type A431 Vehicle control IFNgamma (0 ng/mL, 16 h) cell lysate at 20 µg

Lane 4:

Tap2 knockout A431 Vehicle control IFNgamma (0 ng/mL, 16 h) cell lysate at 20 µg

Lane 5:

HeLa cell lysate at 20 µg

Secondary

Lanes 1 - 5:

Goat anti-Rabbit IgG H&L 800CW at 1/20000 dilution

Lanes 1 - 5:

Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution

Observed band size: 70 kDa

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• WB

Supplier Data

Western blot - Anti-TAP2 antibody (AB180611)

All lanes:

Western blot - Anti-TAP2 antibody (ab180611) at 1/500 dilution

All lanes:

Human placenta extract

Predicted band size: 76 kDa

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