Anti-TAP2 antibody [EPR26237-82]

• IHC

Supplier Data

Immunohistochemistry - Anti-TAP2 antibody [EPR26237-82] (AB307282)

Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labeling TAP2 with ab307282 at 1/2000 (0.257 ug/ml) followed by a ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Positive staining in human cerebrum. The section was incubated with ab307282 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument.. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

• IHC

Supplier Data

Immunohistochemistry - Anti-TAP2 antibody [EPR26237-82] (AB307282)

Immunohistochemical analysis of paraffin-embedded human breast cancer tissue labeling TAP2 with ab307282 at 1/2000 (0.257 ug/ml) followed by ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Positive staining in human breast cancer (PMID : 22065046). The section was incubated with ab307282 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

• IHC

Supplier Data

Immunohistochemistry - Anti-TAP2 antibody [EPR26237-82] (AB307282)

Immunohistochemical analysis of paraffin-embedded human liver tissue labeling TAP2 with ab307282 at 1/2000 (0.257 ug/ml) followed by ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Positive staining in human liver. The section was incubated with ab307282 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

• IHC

Supplier Data

Immunohistochemistry - Anti-TAP2 antibody [EPR26237-82] (AB307282)

Immunohistochemical analysis of paraffin-embedded human breast tissue labeling TAP2 with ab307282 at 1/2000 (0.257 ug/ml) followed by ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Positive staining in human breast. The section was incubated with ab307282 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

• IHC

Supplier Data

Immunohistochemistry - Anti-TAP2 antibody [EPR26237-82] (AB307282)

Immunohistochemical analysis of paraffin-embedded cell pellets labeling TAP2 with ab307282 at 1/2000 (0.257 ug/ml) followed by ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Positive staining in wild-type HeLa cell pellet and no staining in TAP2 knockout HeLa cell pellet. The section was incubated with ab307282 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

• IHC

Supplier Data

Immunohistochemistry - Anti-TAP2 antibody [EPR26237-82] (AB307282)

Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling TAP2 with ab307282 at 1/2000 (0.257 ug/ml) followed by ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Positive staining in human tonsil. The section was incubated with ab307282 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

• IHC

Supplier Data

Immunohistochemistry - Anti-TAP2 antibody [EPR26237-82] (AB307282)

Immunohistochemical analysis of paraffin-embedded human kidney tissue labeling TAP2 with ab307282 at 1/2000 (0.257 ug/ml) followed by ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Positive staining in human kidney. The section was incubated with ab307282 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

• Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-TAP2 antibody [EPR26237-82] (AB307282)

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized Wild-type HeLa (human cervix adenocarcinoma epithelial cell, Right) / TAP2 knockout HeLa (Left) cells labeling TAP2 with ab307282 at 1/500 dilution (0.1ug) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/2000 dilution was used as the secondary antibody. Positive staining on HeLa cells (ab255928), while no staining on TAP2 knockout HeLa cells (ab265426).

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-TAP2 antibody [EPR26237-82] (AB307282)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized TAP2 KO HeLa (TAP2 knockout human cervical adenocarcinoma epithelial cell) ( ab265426) cells labeling TAP2 with ab307282 at 1/500 (1.028 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2 ug/ml) dilution (Green). Confocal image showing endoplasmic reticulum staining in wildtype HeLa cells and no staining in TAP2 knockout HeLa cells. Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8). ab12223 Anti-KDEL mouse monoclonal antibody was used to counterstain tubulin at 1/200 dilution (5 ug/ml) dilution, followed by ab150120 Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) at a 1/1000 dilution (2 ug/ml) (Red). The nuclear counterstain was DAPI (Blue). Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/ml) dilution.

• IP

Supplier Data

Immunoprecipitation - Anti-TAP2 antibody [EPR26237-82] (AB307282)

TAP2 was immunoprecipitated from 0.35 mg HeLa (human epithelial cell line from cervix adenocarcinoma) treated with 10 ng/ml IFN gamma for 16 hours, whole cell lysate 10 ug with ab307282 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab307282 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution. Lane 1 : HeLa treated with 10 ng/ml IFN gamma for 16 hours, whole cell lysate 10 ug Lane 2 : ab307282 IP in HeLa treated with 10 ng/ml IFN gamma for 16 hours, whole cell lysate Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab307282 in HeLa treated with 10ng/ml IFN gamma for 16 hours, whole cell lysate Blocking and dilution buffer and concentration : 5% NFDM/TBST. Exposure time : 1 seconds. Input is non-boiled as boiling may cause protein aggregates.

All lanes:

Immunoprecipitation - Anti-TAP2 antibody [EPR26237-82] (ab307282) at 1/1000 dilution

Lane 1:

HeLa (human epithelial cell line from cervix adenocarcinoma) treated with 10 ng/ml IFN gamma for 16 hours, whole cell lysate 10 μg

Lane 2:

HeLa treated with 10 ng/ml IFN gamma for 16 hours, whole cell lysate

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/5000 dilution

Observed band size: 75 kDa

false

Exposure time: 1s

• WB

Supplier Data

Western blot - Anti-TAP2 antibody [EPR26237-82] (AB307282)

Samples are non-boiled as boiling may cause protein aggregates. The expression of TAP2 is upregulated in response to IFN gamma treatment (PMID : 1946428). Blocking/dilution buffer : 5% NFDM/TBST. Exposure time : 26 seconds.

All lanes:

Western blot - Anti-TAP2 antibody [EPR26237-82] (ab307282) at 1/1000 dilution

Lane 1:

Wild-type HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate 20 μg

Lane 2:

TAP2 knockout HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate 20 μg

Lane 3:

Jurkat (human T cell leukemia T lymphocyte from peripheral blood) whole cell lysate 20 μg

Lane 4:

Raji (human Burkitts lymphoma B lymphocyte) whole cell lysate 20 μg

Lane 5:

Untreated HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate 20 μg

Lane 6:

HeLa treated with /ml IFN gamma for 16 hours whole cell lysate 20 μg

Lane 7:

MOLT-4 (human lymphoblastic leukemia T lymphoblast) whole cell lysate 20 μg

Lane 8:

HT-1080 (human fibrosarcoma epithelial cell) whole cell lysate 20 μg

Lane 9:

Human tonsil tissue lysate 20 μg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 75 kDa

false

Exposure time: 26s