AB249797

Anti-TAPP-1 antibody [EPR12061] - BSA and Azide free

RabMAb
Recombinant
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(1 Publication)

Rabbit Recombinant Monoclonal TAPP-1 antibody. Carrier free. Suitable for IP, WB and reacts with Human samples. Cited in 1 publication.

View Alternative Names

TAPP1, PLEKHA1, Pleckstrin homology domain-containing family A member 1, PH domain-containing family A member 1, Tandem PH domain-containing protein 1, TAPP-1

2 Images

Supplier Data

Immunoprecipitation - Anti-TAPP-1 antibody [EPR12061] - BSA and Azide free (AB249797)

This data was developed using ab174292, the same antibody clone in a different buffer formulation.

Western blot analysis on immunoprecipitation pellet from (1) fetal lung lysate or (2) 1XPBS (negative control) using ab172627, and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.

Lane 1 Primary : ab172627, 1/1000 dilution. Sample : Fetal lung lysate.

Lane 2 Primary : ab172627, 1/1000 dilution. Sample : 1X PBS (negative control).

All lanes:

Immunoprecipitation - Anti-TAPP-1 antibody [EPR12061] (<a href='/en-us/products/primary-antibodies/tapp-1-antibody-epr12061-ab174292'>ab174292</a>) at 1/10 dilution

Lane 1:

MOLT4 cell lysate

Lane 2:

PBS

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG

Predicted band size: 46 kDa

false

Supplier Data

Western blot - Anti-TAPP-1 antibody [EPR12061] - BSA and Azide free (AB249797)

This data was developed using ab174292, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-TAPP-1 antibody [EPR12061] (<a href='/en-us/products/primary-antibodies/tapp-1-antibody-epr12061-ab174292'>ab174292</a>) at 1/1000 dilution

Lane 1:

MOLT4 cell lysate at 10 µg

Lane 2:

HeLa cell lysate at 10 µg

Lane 3:

Jurkat cell lysate at 10 µg

Lane 4:

A549 cell lysate at 10 µg

Lane 5:

Human fetal thymus cell lysate at 10 µg

Lane 6:

Human skeletal muscle cell lysate at 10 µg

Predicted band size: 46 kDa

false

Unconjugated

Anti-TAPP-1 antibody [EPR12061]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR12061

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

IP, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

ab249797 is the carrier-free version of ab174292.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TAPP-1 also known as tandem PH domain-containing protein 1 is a signaling protein with a molecular weight of approximately 50 kDa. It features tandem PH domains that bind to phosphoinositides specifically PI(34)P2 which is a phospholipid signaling molecule. TAPP-1 is expressed in various tissues including the immune system cells such as lymphocytes and other hematopoietic cells. It plays an important role in the context of phosphatidylinositol signaling pathways.

Biological function summary

This protein is important for immune system functions and is involved in cellular signaling processes that govern cell survival proliferation and migration. TAPP-1 can exist as part of larger signaling complexes where it helps to scaffold proteins to phosphoinositide-rich membranes. This interaction directs the spatial and temporal assembly of signaling components critical for immune cell activation and response.

Pathways

TAPP-1 plays a role in PI3K (phosphoinositide 3-kinase) signaling an important pathway in the regulation of the immune response and cellular growth. Its activity impacts the AKT signaling pathway linking it to cell proliferation and survival functions. TAPP-1 works in concert with proteins like SHIP and BTK influencing their activity in the phosphoinositide signaling web that regulates immune cell behavior.

The regulation of TAPP-1 is significant in immune-related disorders and cancers as imbalances in PI3K signaling may lead to these conditions. Aberrant expression or mutation of TAPP-1 has been linked to leukemia where altered phosphoinositide signaling contributes to abnormal cell proliferation. Moreover it shows connection with SHIP through its involvement in immune dysfunctions pointing to a role in diseases like autoimmune disorders where immune signaling pathways are disrupted.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Binds specifically to phosphatidylinositol 3,4-diphosphate (PtdIns3,4P2), but not to other phosphoinositides. May recruit other proteins to the plasma membrane.

See full target information PLEKHA1

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in cell and developmental biology 9:775642 PubMed35004677

2021

HIF-1α Induces HECTD2 Up-Regulation and Aggravates the Malignant Progression of Renal Cell Cancer Repressing miR-320a.

Applications

Unspecified application

Species

Unspecified reactive species

Dong Lv,Taimin Shen,Juncheng Yao,Qi Yang,Ying Xiang,Zhiwei Ma

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-TAPP-1 antibody [EPR12061] - BSA and Azide free

Immunoprecipitation - Anti-TAPP-1 antibody [EPR12061] - BSA and Azide free (AB249797)

Western blot - Anti-TAPP-1 antibody [EPR12061] - BSA and Azide free (AB249797)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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