JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB248976

Anti-TARBP1 antibody [EPR8727] - BSA and Azide free

Be the first to review this product! Submit a review

|

(1 Publication)

Rabbit Recombinant Monoclonal TARBP1 antibody. Carrier free. Suitable for WB and reacts with Human samples. Cited in 1 publication.

View Alternative Names

TRM3, TRP185, TARBP1, tRNA (guanosine(18)-2'-O)-methyltransferase TARBP1, TAR RNA-binding protein 1, TAR RNA-binding protein of 185 kDa, TRP-185

1 Images
Western blot - Anti-TARBP1 antibody [EPR8727] - BSA and Azide free (AB248976)
  • WB

Unknown

Western blot - Anti-TARBP1 antibody [EPR8727] - BSA and Azide free (AB248976)

This data was developed using ab150412, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-TARBP1 antibody [EPR8727] (<a href='/en-us/products/primary-antibodies/tarbp1-antibody-epr8727-ab150412'>ab150412</a>) at 1/1000 dilution

Lane 1:

Jurkat cell lysate at 10 µg

Lane 2:

HeLa cell lysate at 10 µg

Lane 3:

HepG2 cell lysate at 10 µg

Lane 4:

293T cell lysate at 10 µg

Lane 5:

Caco 2 cell lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 182 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR8727

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

Complementary Products

Primary Antibodies

AB60068

Anti-FTSJ2 antibody [2372C6a]

Western blot - Anti-FTSJ2 antibody [2372C6a] (AB60068)

  • 0
  • 0
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB280885

Anti-Protein S antibody [EPR24509-70]

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Protein S antibody [EPR24509-70] (AB280885)

  • 0
  • 0
Primary Antibodies

AB248850

Anti-ZNF575 antibody [EPR9027] - BSA and Azide free

RabMAb|Recombinant

Flow Cytometry (Intracellular) - Anti-ZNF575 antibody [EPR9027] - BSA and Azide free (AB248850)

  • 0
  • 0
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" } } }
style
left-column

Product details

ab248976 is the carrier-free version of ab150412.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TARBP1 also known as RISC-loading complex subunit TARBP1 weighs approximately 314 amino acids in size. It is widely expressed in various tissues with a significant presence in the brain and other areas with high RNA activity. TARBP1 plays a mechanical role in RNA binding and processing. It influences RNA silencing pathways by interacting with specific RNA molecules which regulate gene expression. This protein modulates post-transcriptional gene silencing by forming complexes with other proteins to assist in targeting RNA molecules for degradation or translational repression.
Biological function summary

TARBP1 is involved in the RNA interference (RNAi) pathway contributing to cellular regulatory mechanisms. It associates closely with the RNA-induced silencing complex (RISC) facilitating the recognition and processing of RNA targets. This protein functions as a cofactor aiding AGO2 in cleaving target mRNA an important part of gene silencing processes within the cellular environment. These interactions are vital for maintaining cellular homeostasis and regulating various gene expression pathways through post-transcriptional modifications.

Pathways

TARBP1 is integral to the RNA-induced silencing complex loading pathway and the microRNA (miRNA) biogenesis pathway. In these pathways TARBP1 works in concert with Dicer a ribonuclease enzyme to facilitate the maturation of miRNAs which are pivotal in gene regulation. This interaction determines the gene expression patterns essential for cellular processes such as development and differentiation highlighting TARBP1’s importance in RNA silencing pathways.

TARBP1 has connections to cancer and neurodegenerative diseases. In cancer altered expression levels of TARBP1 can affect miRNA processing leading to dysregulation of genes involved in cell proliferation and apoptosis contributing to tumorigenesis. Similarly misregulation of miRNA activity influenced by TARBP1 has been observed in Alzheimer's disease where imbalanced RNA processing can lead to neuronal dysfunction. Proteins such as AGO2 and Dicer are intricately connected to these conditions as they collaborate with TARBP1 in the regulation of miRNA pathways influencing disease progression and outcomes.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

S-adenosyl-L-methionine-dependent 2'-O-ribose methyltransferase that catalyzes the formation of 2'-O-methylguanosine at position 18 (Gm18) in a subset of tRNA (PubMed : 18412263, PubMed : 31019095, PubMed : 38867004). Selectively mediates Gm18 methylation of tRNAGln-TTG/CTG and tRNASer-TGA/GCT. Gm18 modification can enhance the stability of modified tRNAs (PubMed : 38867004).. (Microbial infection) In case of infection by HIV-1, it binds to the loop region of TAR RNA, a region also bound by RNA polymerase II (PubMed : 7638159, PubMed : 8626763, PubMed : 8846792). Binding of TARBP1 and RNA polymerase II to HIV-1 TAR RNA is mutually exclusive, suggesting that TARBP1 may function alone or in conjunction with HIV-1 Tat to disengage RNA polymerase II from HIV-1 TAR RNA (PubMed : 7638159, PubMed : 8626763, PubMed : 8846792).
See full target information TARBP1
style
left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Oncology reports 50: PubMed37732539

2023

Heat shock protein B7 (HSPB7) inhibits lung adenocarcinoma progression by inhibiting glycolysis.

Applications

Unspecified application

Species

Unspecified reactive species

Zhitao Chen,Peipei Li,Lingguang Shen,Xiuyu Jiang
View all publications
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com