Anti-TARC/CCL17 antibody

• IHC-P

AbReview48572****

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TARC/CCL17 antibody (AB182793)

ab182793 staining TARC/CCL17 in human lung biopsy tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and antigen retrieval was performed by heat mediation in citrate buffer, pH 6.0. Samples were incubated with primary antibody (1/2000 in PBS + 1% BSA) for 16 hours at 4°C. A Biotin-conjugated donkey anti-rabbit IgG polyclonal (1/500) was used as the secondary antibody.

TARC/CCL17 in connective tissue is revealed in brown (DAB impact). Nucleus (and Mucus) are counterstain in blue (Fastblue).

Fixative : Formalin 4% (Qpath), lungs were post-fixed (6h) before paraffin embedded in a tissue-tek^® VIP6 automat.
Sections : 3μm on SuperFrost^®.
Antigen retrieval step : Citrate buffer pH=6 (15 min at 95°C) in a water Bath then slide were placed in Sequenza immunostaining with Coverplate (Shandon).
Endogenous enzyme were blocked with Dual Endogenous Enzyme Block (Dako cytomation) 15min.
Extravidin-HRP was used to increase signal (1/500, 20min, RT).

This image is courtesy of an Abreview submitted by Francois Daubeuf

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TARC/CCL17 antibody (AB182793)

Immunohistochemical analysis of paraffin embedded Human liver cancer tissue labeling TARC/CCL17 with ab182793 at 1/55.

• WB

Unknown

Western blot - Anti-TARC/CCL17 antibody (AB182793)

Gel : 12% SDS-PAGE

Lanes 1, 3 and 4:

Western blot - Anti-TARC/CCL17 antibody (ab182793) at 1/700 dilution

Lane 2:

Western blot - Anti-TARC/CCL17 antibody (ab182793) at 500700 µg

Lane 1:

HepG2 cell lysate at 40 µg

Lane 2:

293T cell lysate at 40 µg

Lane 3:

Human cerebrum tissue lysate at 40 µg

Lane 4:

Mouse kidney tissue lysate at 40 µg

Secondary

All lanes:

Goat anti rabbit IgG at 1/5000 dilution

Predicted band size: 11 kDa

false