Rabbit Recombinant Monoclonal TARC/CCL17 antibody. Suitable for WB and reacts with Mouse samples.
Images
![Western blot - Anti-TARC/CCL17 antibody [EPR28961-9] (AB323800), expandable thumbnail](https://content.abcam.com/products/images/tarc-ccl17-antibody-epr28961-9-ab323800--western-blot-img447086.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-TARC/CCL17 antibody [EPR28961-9] (AB323800)")
![Western blot - Anti-TARC/CCL17 antibody [EPR28961-9] (AB323800), expandable thumbnail](https://content.abcam.com/products/images/tarc-ccl17-antibody-epr28961-9-ab323800--western-blot-img447087.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-TARC/CCL17 antibody [EPR28961-9] (AB323800)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | IHC-P | ICC/IF | |
|---|---|---|---|
| Human | Not recommended | Not recommended | Not recommended |
| Mouse | Tested | Not recommended | Not recommended |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/1000 | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human | Dilution info - | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse | Dilution info - | Notes - |
Target data
Function
Chemokine, which displays chemotactic activity for T lymphocytes, preferentially Th2 cells, but not monocytes or granulocytes. Therefore plays an important role in a wide range of inflammatory and immunological processes (PubMed:10508243, PubMed:10508268). Acts by binding to CCR4 at T-cell surface (By similarity). Mediates GM-CSF/CSF2-driven pain and inflammation (PubMed:27525438). In the brain, required to maintain the typical, highly branched morphology of hippocampal microglia under homeostatic conditions. May be important for the appropriate adaptation of microglial morphology and synaptic plasticity to acute lipopolysaccharide (LPS)-induced neuroinflammation (PubMed:30277599). Plays a role in wound healing, mainly by inducing fibroblast migration into the wound (PubMed:21521373).
Alternative names
Tarc, Ccl17, C-C motif chemokine 17, ABCD-2, CC chemokine TARC, Small-inducible cytokine A17, Thymus and activation-regulated chemokine, TARC/CCL17
Rabbit Recombinant Monoclonal TARC/CCL17 antibody. Suitable for WB and reacts with Mouse samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR28961-9
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
![Western blot - Anti-TARC/CCL17 antibody [EPR28961-9] (ab323800), expandable thumbnail](https://content.abcam.com/products/images/tarc-ccl17-antibody-epr28961-9-ab323800--western-blot-img447086.jpg "Western blot - Anti-TARC/CCL17 antibody [EPR28961-9] (ab323800)")
Western blot - Anti-TARC/CCL17 antibody [EPR28961-9] (ab323800)
TARC/CCL17 Western blot staining using rabbit Anti-TARC/CCL17 antibody
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
The expression of TARC/CCL17 is upregulated in response to M-CSF, GM-CSF and BFA treatment (PMID: 27525438).
In Western blot, Anti-Integrin alpha V antibody [EPR16800] staining at 1/5000 dilution.
All lanes: Western blot - Anti-TARC/CCL17 antibody [EPR28961-9] (ab323800) at 1/1000 dilution
Lane 1: Untreated Mouse bone marrow lysate at 20 µg
Lane 2: Mouse bone marrow treated with 100ng/mL M-CSF for 7 days, 20ng/ml GM-CSF and 300ng/ml BFA for 16 hours lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 11 kDa, 125 kDa
Exposure time: 180s
![Western blot - Anti-TARC/CCL17 antibody [EPR28961-9] (ab323800), expandable thumbnail](https://content.abcam.com/products/images/tarc-ccl17-antibody-epr28961-9-ab323800--western-blot-img447087.jpg "Western blot - Anti-TARC/CCL17 antibody [EPR28961-9] (ab323800)")
Western blot - Anti-TARC/CCL17 antibody [EPR28961-9] (ab323800)
TARC/CCL17 Western blot staining using rabbit Anti-TARC/CCL17 antibody
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
The expression of TARC/CCL17 is upregulated in response to LPS and IL-4 treatment (PMID: 18004070).
The identity of the bands higher than 37 kDa are unknown.
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
All lanes: Western blot - Anti-TARC/CCL17 antibody [EPR28961-9] (ab323800) at 1/1000 dilution
Lane 1: Untreated RAW 264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate at 30 µg
Lane 2: RAW 264.7 treated with 100ng/ml LPS for 24 hours, 10ng/ml IL-4 for 20 hours and 300ng/ml BFA for 4 hours whole cell lysate at 30 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Observed band size: 11 kDa, 36 kDa
Exposure time: 180s
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
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