AB185716

Anti-Tartrate Resistant Acid Phosphatase antibody

Name: Anti-Tartrate Resistant Acid Phosphatase antibody (ab185716) | Abcam
Brand: Abcam Limited
SKU: AB185716
Price: 525 USD
Availability: InStock
Rating: 5 (1 reviews)

(1 Review)

(19 Publications)

Rabbit Polyclonal Tartrate Resistant Acid Phosphatase antibody. Suitable for WB, ICC/IF and reacts with Human, Mouse samples. Cited in 19 publications. Immunogen corresponding to Recombinant Fragment Protein within Human Tartrate-resistant acid phosphatase type 5 aa 50 to C-terminus.

View Alternative Names

Tartrate-resistant acid phosphatase type 5, TR-AP, Tartrate-resistant acid ATPase, Type 5 acid phosphatase, TrATPase, ACP5

2 Images

Immunocytochemistry/ Immunofluorescence - Anti-Tartrate Resistant Acid Phosphatase antibody (AB185716)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Tartrate Resistant Acid Phosphatase antibody (AB185716)

Immunocytochemistry/Immunofluorescence analysis of U2OS cells using ab185716. Blue DAPI for nuclear staining.

Supplier Data

Western blot - Anti-Tartrate Resistant Acid Phosphatase antibody (AB185716)

All lanes:

Western blot - Anti-Tartrate Resistant Acid Phosphatase antibody (ab185716) at 1/500 dilution

Lane 1:

BT474 cell extract

Lane 2:

Jurkat cell extract

Lane 3:

Mouse liver extract

Predicted band size: 37 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

WB, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human Tartrate-resistant acid phosphatase type 5 aa 50 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P13686

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.3 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Tartrate Resistant Acid Phosphatase (TRAP) also known as ACP-5 and type 5 acid phosphatase is an enzyme with mass approximately 35 kDa. It is expressed in osteoclasts macrophages and dendritic cells playing a role in bone resorption and macrophage activation. TRAP exists in both a monomeric and proteolytically activated dimeric form. Its unique tartrate resistance distinguishes it from other acid phosphatases providing specificity for enzyme assays and studies.

Biological function summary

TRAP contributes significantly to bone remodeling through its action in osteoclasts. It helps in degrading mineralized matrix components and may also serve a role in dendritic cell activation signaling and macrophage-related processes. TRAP forms part of a complex mechanism that includes catabolic enzymes which work together to ensure normal skeletal function and immune response.

Pathways

TRAP plays an integral role in the bone resorption pathway along with proteins such as cathepsin K and matrix metalloproteinases. It is involved in osteoclast differentiation necessary for bone turnover and homeostasis. Additionally it participates in the immune response through its activity in macrophages affecting inflammation pathways and innate immune functions.

Abnormal TRAP activity associates with osteopetrosis and osteoporosis. These diseases affect bone density and strength with TRAP often showing elevated levels in conditions of high bone turnover. TRAP activity also connects to inflammatory disorders due to its expression in macrophages linking it to proteins like RANKL and osteoprotegerin which influence bone metabolism and immune signaling.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Involved in osteopontin/bone sialoprotein dephosphorylation. Its expression seems to increase in certain pathological states such as Gaucher and Hodgkin diseases, the hairy cell, the B-cell, and the T-cell leukemias.

See full target information Tartrate-resistant acid phosphatase type 5

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Publications (19)

Recent publications for all applications. Explore the full list and refine your search

Cancer research communications 4:2621-2637 PubMed39248577

2024

Antiangiogenic Tyrosine Kinase Inhibitors have Differential Efficacy in Clear Cell Renal Cell Carcinoma in Bone.

Applications

Unspecified application

Species

Unspecified reactive species

Stefan Maksimovic,Nina C Boscolo,Ludovica La Posta,Sergio Barrios,Mohammad Jad Moussa,Emanuela Gentile,Pedro I Pesquera,Wenjiao Li,Jianfeng Chen,Javier A Gomez,Akshay Basi,Jared K Burks,Christopher Alvarez-Breckenridge,Jianjun Gao,Matthew T Campbell,Eleonora Dondossola

Cell 187:2801-2816.e17 PubMed38657601

2024

Hematopoietic stem cell niche generation and maintenance are distinguishable by an epitranscriptomic program.

Applications

Unspecified application

Species

Unspecified reactive species

Longfei Gao,Heather Lee,Joshua H Goodman,Lei Ding

Cell transplantation 32:9636897231198296 PubMed37710973

2023

Periodontal Tissue Regeneration by Transplantation of Autologous Adipose Tissue-Derived Multi-Lineage Progenitor Cells With Carbonate Apatite.

Applications

Unspecified application

Species

Unspecified reactive species

Masahide Takedachi,Kohsuke Kawasaki,Keigo Sawada,Kazuma Sakura,Mari Murata,Junpei Shimomura,Kazuma Kawakami,Chiaki Morimoto,Koji Miki,Noboru Takeshita,Tomoaki Iwayama,Hanayuki Okura,Akifumi Matsuyama,Masahiro Saito,Masahiro Kitamura,Shinya Murakami

Biomolecules 12: PubMed36139058

2022

Polyphenolic Compounds Inhibit Osteoclast Differentiation While Reducing Autophagy through Limiting ROS and the Mitochondrial Membrane Potential.

Applications

Unspecified application

Species

Unspecified reactive species

Dipranjan Laha,Jaganmay Sarkar,Jyotirindra Maity,Asmita Pramanik,Md Sariful Islam Howlader,Derek Barthels,Hiranmoy Das

Nature communications 13:2654 PubMed35551452

2022

Runx1 and Runx2 inhibit fibrotic conversion of cellular niches for hematopoietic stem cells.

Applications

Unspecified application

Species

Unspecified reactive species

Yoshiki Omatsu,Shota Aiba,Tomonori Maeta,Kei Higaki,Kazunari Aoki,Hitomi Watanabe,Gen Kondoh,Riko Nishimura,Shu Takeda,Ung-Il Chung,Takashi Nagasawa

Cell reports 39:110750 PubMed35508129

2022

SM22α-lineage niche cells regulate intramembranous bone regeneration via PDGFRβ-triggered hydrogen sulfide production.

Applications

Unspecified application

Species

Unspecified reactive species

Xueman Zhou,Jin Liu,Yingcheng Zheng,Zhenzhen Zhang,Yange Wu,Wenke Yang,Jiaqi Liu,Yanmei Huang,Yating Yi,Zhihe Zhao,Hengyi Xiao,Xianming Mo,Jun Wang

Bone research 9:47 PubMed34719673

2021

An antibody against Siglec-15 promotes bone formation and fracture healing by increasing TRAP mononuclear cells and PDGF-BB secretion.

Applications

Unspecified application

Species

Unspecified reactive species

Gehua Zhen,Yang Dan,Ruomei Wang,Ce Dou,Qiaoyue Guo,Melissa Zarr,Linda N Liu,Lieping Chen,Ruoxian Deng,Yusheng Li,Zengwu Shao,Xu Cao

Journal of cellular and molecular medicine 25:2390-2403 PubMed33511706

2021

Dental pulp-derived stem cells inhibit osteoclast differentiation by secreting osteoprotegerin and deactivating AKT signalling in myeloid cells.

Applications

Unspecified application

Species

Unspecified reactive species

Suman Kanji,Ripon Sarkar,Asmita Pramanik,Sudhir Kshirsagar,Carl J Greene,Hiranmoy Das

Frontiers in molecular biosciences 7:585056 PubMed33324677

2020

Osteoblast and Osteoclast Activity Affect Bone Remodeling Upon Regulation by Mechanical Loading-Induced Leukemia Inhibitory Factor Expression in Osteocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Jingke Du,Jiancheng Yang,Zihao He,Junqi Cui,Yiqi Yang,Mingming Xu,Xinhua Qu,Ning Zhao,Mengning Yan,Hanjun Li,Zhifeng Yu

In vivo (Athens, Greece) 34:3291-3299 PubMed33144436

2020

Does the Addition of Abiraterone to Castration Affect the Reduction in Bone Mineral Density?

Applications

Unspecified application

Species

Unspecified reactive species

Shiori Nakajima,Takamitsu Inoue,Mingguo Huang,Koichiro Takayama,Soki Kashima,Ryohei Yamamoto,Atsushi Koizumi,Taketoshi Nara,Kazuyuki Numakura,Mitsuru Saito,Shintaro Narita,Masatomo Miura,Shigeru Satoh,Tomonori Habuchi

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Tartrate Resistant Acid Phosphatase antibody

Immunocytochemistry/ Immunofluorescence - Anti-Tartrate Resistant Acid Phosphatase antibody (AB185716)

Western blot - Anti-Tartrate Resistant Acid Phosphatase antibody (AB185716)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (19)

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