AB212723

Anti-Tartrate Resistant Acid Phosphatase antibody [ACP5/1070] - BSA and Azide free

Name: Anti-Tartrate Resistant Acid Phosphatase antibody [ACP5/1070] - BSA and Azide free (ab212723) | Abcam
Brand: Abcam Limited
SKU: AB212723
Price: 905 USD
Availability: InStock

Be the first to review this product! Submit a review

(4 Publications)

Mouse Monoclonal Tartrate Resistant Acid Phosphatase antibody. Carrier free. Suitable for IHC-P and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human Tartrate-resistant acid phosphatase type 5.

View Alternative Names

Tartrate-resistant acid phosphatase type 5, TR-AP, Tartrate-resistant acid ATPase, Type 5 acid phosphatase, TrATPase, ACP5

1 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tartrate Resistant Acid Phosphatase antibody [ACP5/1070] - BSA and Azide free (AB212723)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tartrate Resistant Acid Phosphatase antibody [ACP5/1070] - BSA and Azide free (AB212723)

Immunohistochemical analysis of formalin fixed, paraffin embedded Human spleen tissue labeling Tartrate Resistant Acid Phosphatase with ab212723 at 1 μg/ml.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

ACP5/1070

Isotype

IgG2b

Light chain type

kappa

Carrier free

Yes

Reacts with

Human

Applications

IHC-P

applications

Immunogen

Recombinant Full Length Protein corresponding to Human Tartrate-resistant acid phosphatase type 5.

P13686

style

left-column

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "0.5-1 µg/mL", "IHCP-species-notes": "<p></p>" } } }

style

left-column

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A/G

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Tartrate Resistant Acid Phosphatase (TRAP) also known as ACP-5 and type 5 acid phosphatase is an enzyme with mass approximately 35 kDa. It is expressed in osteoclasts macrophages and dendritic cells playing a role in bone resorption and macrophage activation. TRAP exists in both a monomeric and proteolytically activated dimeric form. Its unique tartrate resistance distinguishes it from other acid phosphatases providing specificity for enzyme assays and studies.

Biological function summary

TRAP contributes significantly to bone remodeling through its action in osteoclasts. It helps in degrading mineralized matrix components and may also serve a role in dendritic cell activation signaling and macrophage-related processes. TRAP forms part of a complex mechanism that includes catabolic enzymes which work together to ensure normal skeletal function and immune response.

Pathways

TRAP plays an integral role in the bone resorption pathway along with proteins such as cathepsin K and matrix metalloproteinases. It is involved in osteoclast differentiation necessary for bone turnover and homeostasis. Additionally it participates in the immune response through its activity in macrophages affecting inflammation pathways and innate immune functions.

Abnormal TRAP activity associates with osteopetrosis and osteoporosis. These diseases affect bone density and strength with TRAP often showing elevated levels in conditions of high bone turnover. TRAP activity also connects to inflammatory disorders due to its expression in macrophages linking it to proteins like RANKL and osteoprotegerin which influence bone metabolism and immune signaling.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Involved in osteopontin/bone sialoprotein dephosphorylation. Its expression seems to increase in certain pathological states such as Gaucher and Hodgkin diseases, the hairy cell, the B-cell, and the T-cell leukemias.

See full target information Tartrate-resistant acid phosphatase type 5

style

left-column

Publications (4)

Recent publications for all applications. Explore the full list and refine your search

ACS biomaterials science & engineering 7:2392-2407 PubMed33625829

2021

Cellular and Molecular Dynamics during Early Oral Osseointegration: A Comprehensive Characterization in the Lewis Rat.

Applications

Unspecified application

Species

Unspecified reactive species

Sutton E Wheelis,Claudia C Biguetti,Shruti Natarajan,Alexandra Arteaga,Jihad El Allami,Bhuvana Lakkasettar Chandrashekar,Gustavo P Garlet,Danieli C Rodrigues

American journal of physiology. Cell physiology 319:C21-C33 PubMed32374679

2020

Osteoclast-derived exosomal let-7a-5p targets Smad2 to promote the hypertrophic differentiation of chondrocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Jingjin Dai,Rui Dong,Xinyun Han,Jianmei Li,Xiaoshan Gong,Yun Bai,Fei Kang,Mengmeng Liang,Fanchun Zeng,Zhiyong Hou,Shiwu Dong

Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research 35:1188-1202 PubMed32078184

2020

Glucocorticoids Disrupt Skeletal Angiogenesis Through Transrepression of NF-κB-Mediated Preosteoclast Pdgfb Transcription in Young Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Yi Peng,Shan Lv,Yusheng Li,Jianxi Zhu,Shijie Chen,Gehua Zhen,Xu Cao,Song Wu,Janet L Crane

The Journal of clinical investigation 129:2578-2594 PubMed30946695

2019

Macrophage-lineage TRAP+ cells recruit periosteum-derived cells for periosteal osteogenesis and regeneration.

Applications

Unspecified application

Species

Unspecified reactive species

Bo Gao,Ruoxian Deng,Yu Chai,Hao Chen,Bo Hu,Xiao Wang,Shouan Zhu,Yong Cao,Shuangfei Ni,Mei Wan,Liu Yang,Zhuojing Luo,Xu Cao

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Tartrate Resistant Acid Phosphatase antibody [ACP5/1070] - BSA and Azide free

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tartrate Resistant Acid Phosphatase antibody [ACP5/1070] - BSA and Azide free (AB212723)

Key facts

Host species

Clonality

Clone number

Isotype

Light chain type

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

Product promise