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Rabbit Recombinant Monoclonal HTSF1 antibody. Suitable for WB, IP, ICC/IF, IHC-P and reacts with Human, Rat samples.

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Images

Western blot - Anti-Tat-SF1 antibody [EPR9105(B)] (AB134921), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tat-SF1 antibody [EPR9105(B)] (AB134921), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tat-SF1 antibody [EPR9105(B)] (AB134921), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-Tat-SF1 antibody [EPR9105(B)] (AB134921), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tat-SF1 antibody [EPR9105(B)] (AB134921), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIPICC/IFIHC-PFlow Cyt
Human
Tested
Expected
Tested
Tested
Not recommended
Rat
Expected
Expected
Expected
Expected
Not recommended

Tested
Tested

Species
Human
Dilution info
1/1000 - 1/10000
Notes

-

Expected
Expected

Species
Rat
Dilution info
1/1000 - 1/10000
Notes

-

Expected
Expected

Species
Human
Dilution info
1/10 - 1/100
Notes

-

Species
Rat
Dilution info
1/10 - 1/100
Notes

-

Tested
Tested

Species
Human
Dilution info
1/250 - 1/500
Notes

-

Expected
Expected

Species
Rat
Dilution info
1/250 - 1/500
Notes

-

Tested
Tested

Species
Human
Dilution info
1/100 - 1/250
Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Expected
Expected

Species
Rat
Dilution info
1/100 - 1/250
Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species
Human, Rat
Dilution info
-
Notes

-

Associated Products

Select an associated product type

1 product for Alternative Product

Target data

Function

Component of the 17S U2 SnRNP complex of the spliceosome, a large ribonucleoprotein complex that removes introns from transcribed pre-mRNAs (PubMed:30567737, PubMed:32494006, PubMed:34822310). The 17S U2 SnRNP complex (1) directly participates in early spliceosome assembly and (2) mediates recognition of the intron branch site during pre-mRNA splicing by promoting the selection of the pre-mRNA branch-site adenosine, the nucleophile for the first step of splicing (PubMed:30567737, PubMed:32494006, PubMed:34822310). Within the 17S U2 SnRNP complex, HTATSF1 is required to stabilize the branchpoint-interacting stem loop (PubMed:34822310). HTATSF1 is displaced from the 17S U2 SnRNP complex before the stable addition of the 17S U2 SnRNP complex to the spliceosome, destabilizing the branchpoint-interacting stem loop and allowing to probe intron branch site sequences (PubMed:32494006, PubMed:34822310). Also acts as a regulator of transcriptional elongation, possibly by mediating the reciprocal stimulatory effect of splicing on transcriptional elongation (PubMed:10454543, PubMed:10913173, PubMed:11780068). Involved in double-strand break (DSB) repair via homologous recombination in S-phase by promoting the recruitment of TOPBP1 to DNA damage sites (PubMed:35597237). Mechanistically, HTATSF1 is (1) recruited to DNA damage sites in S-phase via interaction with poly-ADP-ribosylated RPA1 and (2) phosphorylated by CK2, promoting recruitment of TOPBP1, thereby facilitating RAD51 nucleofilaments formation and RPA displacement, followed by homologous recombination (PubMed:35597237). (Microbial infection) In case of infection by HIV-1, it is up-regulated by the HIV-1 proteins NEF and gp120, acts as a cofactor required for the Tat-enhanced transcription of the virus.

Alternative names

17S U2 SnRNP complex component HTATSF1, HIV Tat-specific factor 1, Tat-SF1, HTATSF1

Recommended products

Rabbit Recombinant Monoclonal HTSF1 antibody. Suitable for WB, IP, ICC/IF, IHC-P and reacts with Human, Rat samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR9105(B)
Purification technique
Affinity purification Protein A
Dissociation constant
8 x 10-12 M
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C

Notes

Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Tat-SF1 also known as transcription elongation factor SPT5 (SPT4-5) is a protein integral to the RNA transcription process. The mass of Tat-SF1 is approximately 90 kDa. It is primarily expressed in the nucleus of the cell where it interacts with RNA Polymerase II to facilitate the elongation phase of transcription. Tat-SF1 plays a significant role by enhancing the transcriptional speed and efficiency critical for the production of various RNA species.

Biological function summary

Tat-SF1 functions by participating in the regulation of transcription elongation. It is part of the larger multi-protein complex involving CycT1 another important transcription factor. This complex helps modulate the interaction of RNA polymerase II with other transcriptional machinery and influences the synthesis of mRNA. Through these actions Tat-SF1 supports the accurate gene expression important for normal cellular processes and response to environmental cues.

Pathways

Tat-SF1 has functions within the general transcriptional regulation pathway and the HIV-1 viral transcription pathway. It directly interacts with the HIV-1 Tat protein; this interaction is essential for efficient transcription of HIV-1 genes. The connection with the CycT1 protein is important within these pathways as it aids in recruiting Tat-SF1 to the sites where RNA polymerase II needs support therefore coordinating a precise transcriptional response.

Associated diseases and disorders

Tat-SF1 is notably linked to HIV-1 infection due to its interaction with the viral Tat protein facilitating viral replication. Disruption or alteration in the function of Tat-SF1 can hinder this process making it a target for therapeutic interventions. Additionally any dysregulation in the normal transcriptional process involving Tat-SF1 and CycT1 can contribute to oncogenesis as improper gene expression can lead to unregulated cell proliferation. Understanding these connections highlights the importance of Tat-SF1 in disease mechanisms and therapeutic strategy development.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

7 product images

  • Western blot - Anti-Tat-SF1 antibody [EPR9105(B)] (ab134921), expandable thumbnail

    Western blot - Anti-Tat-SF1 antibody [EPR9105(B)] (ab134921)

    All lanes: Western blot - Anti-Tat-SF1 antibody [EPR9105(B)] (ab134921) at 1/1000 dilution

    Lane 1: HeLa cell lysate at 10 µg

    Lane 2: Jurkat cell lysate at 10 µg

    Secondary

    All lanes: Goat-anti-rabbit HRP at 1/2000 dilution

    Developed using the ECL technique.

    Predicted band size: 86 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tat-SF1 antibody [EPR9105(B)] (ab134921), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tat-SF1 antibody [EPR9105(B)] (ab134921)

    Immunohistochemical analysis of paraffin-embedded Human colon tissue labelling Tat-SF1 with ab134921 at 1/100 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tat-SF1 antibody [EPR9105(B)] (ab134921), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tat-SF1 antibody [EPR9105(B)] (ab134921)

    Immunohistochemical analysis of paraffin-embedded Human kidney tissue labelling Tat-SF1 with ab134921 at 1/100 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-Tat-SF1 antibody [EPR9105(B)] (ab134921), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Tat-SF1 antibody [EPR9105(B)] (ab134921)

    Immunofluorescence analysis of HepG2 cells staining Tat-SF1 with ab134921 at 1/250 dilution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tat-SF1 antibody [EPR9105(B)] (ab134921), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tat-SF1 antibody [EPR9105(B)] (ab134921)

    Immunohistochemical analysis using ab134921 showing positive staining in Normal testis tissue.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tat-SF1 antibody [EPR9105(B)] (ab134921), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tat-SF1 antibody [EPR9105(B)] (ab134921)

    Immunohistochemical analysis using ab134921 showing positive staining in Breast carcinoma tissue.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • OI-RD Scanning - Anti-Tat-SF1 antibody [EPR9105(B)] (ab134921), expandable thumbnail

    OI-RD Scanning - Anti-Tat-SF1 antibody [EPR9105(B)] (ab134921)

    We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody.
    Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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