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Mouse Monoclonal TATA binding protein TBP antibody. Suitable for ChIP, ChIP/Chip, ICC, IP, Flow Cyt, ELISA, WB, IHC-P, ICC/IF, EMSA, IHC-Fr and reacts with Human, Rat, Mouse samples. Cited in 263 publications. Immunogen corresponding to Synthetic Peptide within Human TBP.
Alternative names=TATA-box-binding protein, TATA sequence-binding protein, TATA-binding factor, TATA-box factor, Transcription initiation factor TFIID TBP subunit, TF2D, TBP, GTF2D1, TFIID
- Isotype
IgG1
- Host species
Mouse
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide- Form
Liquid
- Clonality
Monoclonal
- Immunogen
Synthetic Peptide within Human TBP. The exact immunogen used to generate this antibody is proprietary information. Database link: P20226
Reactivity data
Select an application| ChIP | ChIP/Chip | ICC | IP | Flow Cyt | ELISA | WB | IHC-P | ICC/IF | EMSA | IHC-Fr | |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Human | Tested | Expected | Expected | Expected | Tested | Expected | Expected | Expected | Expected | Expected | Expected |
| Mouse | Predicted | Expected | Expected | Expected | Not recommended | Expected | Expected | Expected | Expected | Expected | Expected |
| Rat | Predicted | Expected | Expected | Expected | Not recommended | Expected | Expected | Expected | Expected | Expected | Expected |
| Drosophila melanogaster | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
| Saccharomyces cerevisiae | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
| Silk worm | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
| Xenopus laevis | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
Images
ChIP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 5.00000-10.00000 µg for 25 µg chromatin | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Saccharomyces cerevisiae, Silk worm, Drosophila melanogaster, Xenopus laevis | Dilution info - | Notes - |
ChIP/Chip
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Rat, Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Saccharomyces cerevisiae, Silk worm, Drosophila melanogaster, Xenopus laevis | Dilution info - | Notes - |
ICC
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Saccharomyces cerevisiae, Silk worm, Drosophila melanogaster, Xenopus laevis | Dilution info - | Notes - |
IP
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Saccharomyces cerevisiae, Silk worm, Drosophila melanogaster, Xenopus laevis | Dilution info - | Notes - |
Flow Cyt
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1.00000-6.00000 µg for 106 Cells | Notes ab170190 and ab81216 - Mouse monoclonal IgG1, are suitable for use as an isotype control with this antibody. We recommend Goat Anti-Mouse IgG H&L (DyLight® 488) preadsorbed (ab96879) secondary antibody |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Drosophila melanogaster | Dilution info - | Notes ab170190 and ab81216 - Mouse monoclonal IgG1, are suitable for use as an isotype control with this antibody. We recommend Goat Anti-Mouse IgG H&L (DyLight® 488) preadsorbed (ab96879) secondary antibody |
Species Xenopus laevis | Dilution info - | Notes ab170190 and ab81216 - Mouse monoclonal IgG1, are suitable for use as an isotype control with this antibody. We recommend Goat Anti-Mouse IgG H&L (DyLight® 488) preadsorbed (ab96879) secondary antibody |
Species Mouse | Dilution info - | Notes - |
Species Rat | Dilution info - | Notes - |
Species Saccharomyces cerevisiae | Dilution info - | Notes - |
Species Silk worm | Dilution info - | Notes - |
ELISA
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes We recommend Rabbit Anti-Mouse IgG H&;L (Alkaline Phosphatase) (ab6729) secondary antibody. |
Species Rat | Dilution info - | Notes We recommend Rabbit Anti-Mouse IgG H&;L (Alkaline Phosphatase) (ab6729) secondary antibody. |
Species Human | Dilution info - | Notes We recommend Rabbit Anti-Mouse IgG H&;L (Alkaline Phosphatase) (ab6729) secondary antibody. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Saccharomyces cerevisiae | Dilution info - | Notes We recommend Rabbit Anti-Mouse IgG H&;L (Alkaline Phosphatase) (ab6729) secondary antibody. |
Species Silk worm | Dilution info - | Notes We recommend Rabbit Anti-Mouse IgG H&;L (Alkaline Phosphatase) (ab6729) secondary antibody. |
Species Drosophila melanogaster | Dilution info - | Notes We recommend Rabbit Anti-Mouse IgG H&;L (Alkaline Phosphatase) (ab6729) secondary antibody. |
Species Xenopus laevis | Dilution info - | Notes We recommend Rabbit Anti-Mouse IgG H&;L (Alkaline Phosphatase) (ab6729) secondary antibody. |
WB
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Saccharomyces cerevisiae, Silk worm, Drosophila melanogaster, Xenopus laevis | Dilution info - | Notes - |
IHC-P
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Saccharomyces cerevisiae, Silk worm, Drosophila melanogaster, Xenopus laevis | Dilution info - | Notes - |
ICC/IF
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Saccharomyces cerevisiae, Silk worm, Drosophila melanogaster, Xenopus laevis | Dilution info - | Notes - |
EMSA
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Saccharomyces cerevisiae, Silk worm, Drosophila melanogaster, Xenopus laevis | Dilution info - | Notes - |
IHC-Fr
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Saccharomyces cerevisiae, Silk worm, Drosophila melanogaster, Xenopus laevis | Dilution info - | Notes - |
Recommended products
Mouse Monoclonal TATA binding protein TBP antibody. Suitable for ChIP, ChIP/Chip, ICC, IP, Flow Cyt, ELISA, WB, IHC-P, ICC/IF, EMSA, IHC-Fr and reacts with Human, Rat, Mouse samples. Cited in 263 publications. Immunogen corresponding to Synthetic Peptide within Human TBP.
Alternative names=TATA-box-binding protein, TATA sequence-binding protein, TATA-binding factor, TATA-box factor, Transcription initiation factor TFIID TBP subunit, TF2D, TBP, GTF2D1, TFIID
Key facts
- Isotype
IgG1
- Host species
Mouse
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide- Form
Liquid
- Clonality
Monoclonal
- Immunogen
Synthetic Peptide within Human TBP. The exact immunogen used to generate this antibody is proprietary information. Database link: P20226
- Clone number
1TBP18
- Purification technique
Affinity purification Protein G
- Epitope
Within amino acid residues 1-20 of human, mouse and rat TBP.The specific epitope for this antibody is accessible when the C-terminal domain of TBP is removed or when TBP is complexed with DNA.
- Concentration
- Purification notes
Purified from TCS
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
This product was changed from ascites to tissue culture supernatant on 19/12/2018. Please note that the dilutions may need to be adjusted accordingly. If you have any questions please do not hesitate to contact our scientific support team.
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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