AB28175

Anti-TATA binding protein TBP antibody

Name: Anti-TATA binding protein TBP antibody (ab28175) | Abcam
Brand: Abcam Limited
SKU: AB28175
Price: 400 USD
Availability: InStock
Rating: 4 (3 reviews)

Lab Essentials
What is this?

(3 Reviews)

(22 Publications)

Rabbit Polyclonal TATA binding protein TBP antibody. Suitable for ChIP and reacts with Human samples. Cited in 22 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human TBP.

View Alternative Names

GTF2D1, TF2D, TFIID, TBP, TATA-box-binding protein, TATA sequence-binding protein, TATA-binding factor, TATA-box factor, Transcription initiation factor TFIID TBP subunit

2 Images

ChIP - Anti-TATA binding protein TBP antibody (AB28175)

ChIP

Unknown

ChIP - Anti-TATA binding protein TBP antibody (AB28175)

Chromatin was prepared from Hela cells according to the Abcam X-ChIP protocol. Cells were fixed with formaldehyde for 10min. The ChIP was performed with 25μg of chromatin, 8μg of ab28175 (blue), and 20μl of Protein A/G sepharose beads. No antibody was added to the beads control (yellow). The immunoprecipitated DNA was quantified by real time PCR (Taqman and sybr green approach). Primers and probes are located either in the core promoter of the gene (TATA) or in the open reading frame (ORF).

ChIP

AbReview32840****

ChIP - Anti-TATA binding protein TBP antibody (AB28175)

ChIP analysis using ab28175 binding TATA binding protein TBP in mouse liver nuclear tissue lysate. Cells were cross-linked for 10 minutes with 1% paraformaldehyde. Samples were incubated with primary antibody for 16 hours at 4°C in TE pH8, 150mM NaCl, Trition 1%, SDS 0,1%. Protein binding was detected using real-time PCR.
Negative Control : beads.

This image is courtesy of an anonymous Abreview

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ChIP

applications

Immunogen

Recombinant Full Length Protein corresponding to Human TBP.

P20226

Specificity

Ab28175 recognises TATA binding protein TBP.

style

left-column

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ChIP" : {"fullname" : "ChIP", "shortname":"ChIP"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ChIP-species-checked": "testedAndGuaranteed", "ChIP-species-dilution-info": "5-10 µg for 25 µg chromatin", "ChIP-species-notes": "<p></p>" } } }

style

left-column

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.9 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The TATA binding protein (TBP) also known as TBP or TFIID (Transcription Factor IID) is a central player in transcription initiation. This protein weighing approximately 38 kDa binds to the TATA box a DNA sequence found in the promoter region of many genes especially those transcribed by RNA polymerase II. TBP facilitates the loading of the transcription machinery onto DNA enabling the start of transcription. It is found inside the nucleus of eukaryotic cells present across diverse cell types owing to its role in gene expression.

Biological function summary

TBP serves as a pivotal anchor in the assembly of the transcription preinitiation complex contributing to the process by bending DNA to assist other transcription factors such as TFIIA and TFIIB in binding. Its role in this complex illustrates its importance in regulating gene expression at a fundamental level. TBP’s interaction with other transcription factors ensures precise transcription regulation reflecting its significant contribution to cellular activities.

Pathways

TBP acts within the RNA polymerase II transcription initiation pathway and is integral in regulating gene expression. TBP's involvement is critical for the process of starting the transcription of mRNA. In its pathway TBP interacts with proteins like TFIID helping to anchor other transcription factors in the complex. By collaborating with these proteins TBP shapes gene regulatory networks essential for a wide range of cellular functions.

TBP is linked to conditions such as spinocerebellar ataxia type 17 (SCA17) and Huntington's disease. SCA17 is associated with mutations in the TBP gene that disrupt its usual functioning leading to neurodegeneration. In Huntington's disease TBP's interaction with proteins such as huntingtin may influence nuclear signaling pathways involved in disease progression. The understanding of TBP’s role in these disorders underlines its relevance in research focused on genetic diseases and transcription-related pathologies.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

The TFIID basal transcription factor complex plays a major role in the initiation of RNA polymerase II (Pol II)-dependent transcription (PubMed : 33795473). TFIID recognizes and binds promoters with or without a TATA box via its subunit TBP, a TATA-box-binding protein, and promotes assembly of the pre-initiation complex (PIC) (PubMed : 2194289, PubMed : 2363050, PubMed : 2374612, PubMed : 27193682, PubMed : 33795473). The TFIID complex consists of TBP and TBP-associated factors (TAFs), including TAF1, TAF2, TAF3, TAF4, TAF5, TAF6, TAF7, TAF8, TAF9, TAF10, TAF11, TAF12 and TAF13 (PubMed : 27007846, PubMed : 33795473). The TFIID complex structure can be divided into 3 modules TFIID-A, TFIID-B, and TFIID-C (PubMed : 33795473). TBP forms the TFIID-A module together with TAF3 and TAF5 (PubMed : 33795473). TBP is a general transcription factor that functions at the core of the TFIID complex (PubMed : 2194289, PubMed : 2363050, PubMed : 2374612, PubMed : 27193682, PubMed : 33795473, PubMed : 9836642). During assembly of the core PIC on the promoter, as part of TFIID, TBP binds to and also bends promoter DNA, irrespective of whether the promoter contains a TATA box (PubMed : 33795473). Component of a BRF2-containing transcription factor complex that regulates transcription mediated by RNA polymerase III (PubMed : 26638071). Component of the transcription factor SL1/TIF-IB complex, which is involved in the assembly of the PIC during RNA polymerase I-dependent transcription (PubMed : 15970593). The rate of PIC formation probably is primarily dependent on the rate of association of SL1 with the rDNA promoter (PubMed : 15970593). SL1 is involved in stabilization of nucleolar transcription factor 1/UBTF on rDNA (PubMed : 15970593).

See full target information TBP

style

left-column

Publications (22)

Recent publications for all applications. Explore the full list and refine your search

PloS one 19:e0306633 PubMed39208271

2024

Taenia solium TAF6 and TAF9 bind to a downstream promoter element present in the Tstbp1 gene core promoter.

Applications

Unspecified application

Species

Unspecified reactive species

Oscar Rodríguez-Lima,Ponciano García-Gutiérrez,Lucía Jiménez,Laura A Velázquez-Villegas,Angel Zarain-Herzberg,Roberto Lazzarini,Karel Estrada,Abraham Landa

Science advances 10:eadl4893 PubMed39121214

2024

3D chromatin architecture, BRD4, and Mediator have distinct roles in regulating genome-wide transcriptional bursting and gene network.

Applications

Unspecified application

Species

Unspecified reactive species

Pawel Trzaskoma,SeolKyoung Jung,Aleksandra Pękowska,Christopher H Bohrer,Xiang Wang,Faiza Naz,Stefania Dell'Orso,Wendy D Dubois,Ana Olivera,Supriya V Vartak,Yongbing Zhao,Subhashree Nayak,Andrew Overmiller,Maria I Morasso,Vittorio Sartorelli,Daniel R Larson,Carson C Chow,Rafael Casellas,John J O'Shea

Heliyon 8:e11656 PubMed36458309

2022

PEITC: A resounding molecule averts metastasis in breast cancer cells by regulating PKCδ/Aurora A interplay.

Applications

Unspecified application

Species

Unspecified reactive species

Souvick Biswas,Elizabeth Mahapatra,Salini Das,Madhumita Roy,Sutapa Mukherjee

Human molecular genetics 32:104-121 PubMed35925837

2022

TFIIB-related factor 1 is a nucleolar protein that promotes RNA polymerase I-directed transcription and tumour cell growth.

Applications

Unspecified application

Species

Unspecified reactive species

Juan Wang,Qiyue Chen,Xin Wang,Shasha Zhao,Huan Deng,Baoqiang Guo,Cheng Zhang,Xiaoye Song,Wensheng Deng,Tongcun Zhang,Hongwei Ni

International journal of molecular sciences 22: PubMed34884937

2021

Crosstalk between E-Cadherin/β-Catenin and NF-κB Signaling Pathways: The Regulation of Host-Pathogen Interaction during Leptospirosis.

Applications

Unspecified application

Species

Unspecified reactive species

Shen-Hsing Hsu,Li-Fang Chou,Chung-Hung Hong,Ming-Yang Chang,Chung-Ying Tsai,Ya-Chung Tian,Huang-Yu Yang,Chih-Wei Yang

Nature structural & molecular biology 28:435-442 PubMed33972784

2021

A dual role for H2A.Z.1 in modulating the dynamics of RNA polymerase II initiation and elongation.

Applications

Unspecified application

Species

Unspecified reactive species

Constantine Mylonas,Choongman Lee,Alexander L Auld,Ibrahim I Cisse,Laurie A Boyer

Oncology reports 45:706-716 PubMed33416185

2021

miRNA‑490‑3p promotes the metastatic progression of invasive ductal carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Ning Lu,Mei Zhang,Lu Lu,Yan-Zhao Liu,Hai-Hong Zhang,Xiao-Dong Liu

The Journal of biological chemistry 295:8887-8900 PubMed32434928

2020

The transcriptional corepressor CBFA2T3 inhibits all--retinoic acid-induced myeloid gene expression and differentiation in acute myeloid leukemia.

Applications

Unspecified application

Species

Unspecified reactive species

Nickolas Steinauer,Chun Guo,Jinsong Zhang

International journal of molecular sciences 20: PubMed31277458

2019

The TAFs of TFIID Bind and Rearrange the Topology of the TATA-Less Promoter.

Applications

Unspecified application

Species

Unspecified reactive species

Sarah N Le,Christopher R Brown,Stacy Harvey,Hinrich Boeger,Hans Elmlund,Dominika Elmlund

Molecular oncology 13:1369-1387 PubMed30927552

2019

Promyelocytic leukemia protein (PML) controls breast cancer cell proliferation by modulating Forkhead transcription factors.

Applications

Unspecified application

Species

Unspecified reactive species

Nikoleta Sachini,Panagiota Arampatzi,Antonios Klonizakis,Christoforos Nikolaou,Takis Makatounakis,Eric W-F Lam,Androniki Kretsovali,Joseph Papamatheakis

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-TATA binding protein TBP antibody

ChIP - Anti-TATA binding protein TBP antibody (AB28175)

ChIP - Anti-TATA binding protein TBP antibody (AB28175)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Alternative Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Complementary Products

Primary Antibodies

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (22)

Product promise