Rabbit Recombinant Monoclonal TATA binding protein TBP antibody. Suitable for ChIC/CUT&RUN-seq, IHC-P, IP, ChIP, WB, ICC/IF, ChIP-seq, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples. Cited in 5 publications.
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
ChIC/CUT&RUN-seq | IHC-P | IP | ChIP | WB | ICC/IF | ChIP-seq | Flow Cyt (Intra) | |
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Human | Tested | Tested | Tested | Tested | Tested | Tested | Tested | Tested |
Mouse | Expected | Tested | Expected | Expected | Tested | Expected | Expected | Expected |
Rat | Expected | Tested | Expected | Expected | Tested | Expected | Expected | Expected |
Species | Dilution info | Notes |
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Species Human | Dilution info 3 µg | Notes - |
Species | Dilution info | Notes |
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Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
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Species Mouse | Dilution info 1/4000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Species Rat | Dilution info 1/4000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Species Human | Dilution info 1/4000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/30 | Notes - |
Species | Dilution info | Notes |
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Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 5 µg chromatin for 25 µg chromatin | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info 1/1000 | Notes - |
Species Rat | Dilution info 1/1000 | Notes - |
Species Human | Dilution info 1/1000 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/500 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 4 µg chromatin for 30 µg chromatin | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/500 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
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The TFIID basal transcription factor complex plays a major role in the initiation of RNA polymerase II (Pol II)-dependent transcription (PubMed:33795473). TFIID recognizes and binds promoters with or without a TATA box via its subunit TBP, a TATA-box-binding protein, and promotes assembly of the pre-initiation complex (PIC) (PubMed:2194289, PubMed:2363050, PubMed:2374612, PubMed:27193682, PubMed:33795473). The TFIID complex consists of TBP and TBP-associated factors (TAFs), including TAF1, TAF2, TAF3, TAF4, TAF5, TAF6, TAF7, TAF8, TAF9, TAF10, TAF11, TAF12 and TAF13 (PubMed:27007846, PubMed:33795473). The TFIID complex structure can be divided into 3 modules TFIID-A, TFIID-B, and TFIID-C (PubMed:33795473). TBP forms the TFIID-A module together with TAF3 and TAF5 (PubMed:33795473). TBP is a general transcription factor that functions at the core of the TFIID complex (PubMed:2194289, PubMed:2363050, PubMed:2374612, PubMed:27193682, PubMed:33795473, PubMed:9836642). During assembly of the core PIC on the promoter, as part of TFIID, TBP binds to and also bends promoter DNA, irrespective of whether the promoter contains a TATA box (PubMed:33795473). Component of a BRF2-containing transcription factor complex that regulates transcription mediated by RNA polymerase III (PubMed:26638071). Component of the transcription factor SL1/TIF-IB complex, which is involved in the assembly of the PIC during RNA polymerase I-dependent transcription (PubMed:15970593). The rate of PIC formation probably is primarily dependent on the rate of association of SL1 with the rDNA promoter (PubMed:15970593). SL1 is involved in stabilization of nucleolar transcription factor 1/UBTF on rDNA (PubMed:15970593).
GTF2D1, TF2D, TFIID, TBP, TATA-box-binding protein, TATA sequence-binding protein, TATA-binding factor, TATA-box factor, Transcription initiation factor TFIID TBP subunit
Rabbit Recombinant Monoclonal TATA binding protein TBP antibody. Suitable for ChIC/CUT&RUN-seq, IHC-P, IP, ChIP, WB, ICC/IF, ChIP-seq, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples. Cited in 5 publications.
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
For more information, read more on recombinant antibodies.
The TATA binding protein (TBP) also known as TBP or TFIID (Transcription Factor IID) is a central player in transcription initiation. This protein weighing approximately 38 kDa binds to the TATA box a DNA sequence found in the promoter region of many genes especially those transcribed by RNA polymerase II. TBP facilitates the loading of the transcription machinery onto DNA enabling the start of transcription. It is found inside the nucleus of eukaryotic cells present across diverse cell types owing to its role in gene expression.
TBP serves as a pivotal anchor in the assembly of the transcription preinitiation complex contributing to the process by bending DNA to assist other transcription factors such as TFIIA and TFIIB in binding. Its role in this complex illustrates its importance in regulating gene expression at a fundamental level. TBP’s interaction with other transcription factors ensures precise transcription regulation reflecting its significant contribution to cellular activities.
TBP acts within the RNA polymerase II transcription initiation pathway and is integral in regulating gene expression. TBP's involvement is critical for the process of starting the transcription of mRNA. In its pathway TBP interacts with proteins like TFIID helping to anchor other transcription factors in the complex. By collaborating with these proteins TBP shapes gene regulatory networks essential for a wide range of cellular functions.
TBP is linked to conditions such as spinocerebellar ataxia type 17 (SCA17) and Huntington's disease. SCA17 is associated with mutations in the TBP gene that disrupt its usual functioning leading to neurodegeneration. In Huntington's disease TBP's interaction with proteins such as huntingtin may influence nuclear signaling pathways involved in disease progression. The understanding of TBP’s role in these disorders underlines its relevance in research focused on genetic diseases and transcription-related pathologies.
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We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
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Terms & Conditions.
Chromatin was prepared from HeLa (human epithelial cell line from cervix adenocarcinoma) cells according to the Abcam X-ChIP protocol. The ChIP was performed with 25 μg of chromatin, 5 μg of ab220788 (red), and 20 μl of Protein A/G sepharose beads. 5 μg of rabbit normal IgG was added to the beads control (gray). The immunoprecipitated DNA was quantified by real time PCR (SYBR green approach).Primers and probes are located in the first kb of the transcribed region.
CUT&RUN was performed using the ChIC/CUT&RUN pAG-MNAse ChIC/CUT&RUN pAG-MNase ab285373, 3x10^5 HeLa cells and 3µg of ab220788 [EPR21954]. The resulting DNA was sequenced on the NovaSeq 6000 (PE150).
Additional screenshots of mapped reads can be downloaded here.
Chromatin was prepared from HeLa cells. Cells were fixed with 1% formaldehyde for 10 minutes. ChIP was performed with 30 μg of chromatin and 4 μg of ab220788. ChIP DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 30 million reads. ChIP-Seq validation performed with ChIP-Kit Transcription Factors ChIP-Seq (ChIP Kit (Transcription factors, ChIP-seq) ab270813).
Additional screenshots of mapped reads can be downloaded here.
Chromatin was prepared from HeLa cells. Cells were fixed with 1% formaldehyde for 10 minutes. ChIP was performed with 30 μg of chromatin and 4 μg of Anti-TATA binding protein TBP antibody [EPR21954] - ChIP Grade (ab220788). ChIP DNA was sequenced on the Illumina NextSeq 500 to a depth of 30 million reads. ChIP-Seq validation performed by Active Motif, Carlsbad, CA.
Additional screenshots of mapped reads can be downloaded here.
TATA binding protein TBP Western blot staining using rabbit Anti-TATA binding protein TBP antibody
Blocking and dilution buffer: 5% NFDM/TBST.
Human TBP migrates with an approximate molecular mass of 45 kDa on SDS-PAGE (PMID:1458534; PMID: 1907890) the molecular mass observed is lower in mouse than in human samples due to a shorter sequence.
All lanes: Western blot - Anti-TATA binding protein TBP antibody [EPR21954] - Loading Control and ChIP Grade (ab220788) at 1/1000 dilution
Lane 1: HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 20 µg
Lane 2: HEK-293 (human epithelial cell line from embryonic kidney) whole cell lysate at 20 µg
Lane 3: HCT 116 (human colorectal carcinoma cell line) whole cell lysate at 20 µg
Lane 4: MCF7 (human breast adenocarcinoma cell line) whole cell lysate at 20 µg
Lane 5: C2C12 (mouse myoblast cell line) whole cell lysate at 20 µg
Lane 6: Mouse testis lysate at 20 µg
Lane 7: Rat testis lysate at 20 µg
Lane 8: NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 20 µg
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Predicted band size: 38 kDa
Observed band size: 35 kDa, 45 kDa
TATA binding protein TBP was immunoprecipitated from 0.35 mg of HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate with ab220788 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab220788 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366), was used for detection at 1/5000 dilution.
Lane 1: HeLa whole cell lysate 10 μg (Input).
Lane 2: ab220788 IP in HeLa whole cell lysate.
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab220788 in HeLa whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 30 seconds.
All lanes: Immunoprecipitation - Anti-TATA binding protein TBP antibody [EPR21954] - Loading Control and ChIP Grade (ab220788)
Predicted band size: 38 kDa
Observed band size: 45 kDa
Immunohistochemical analysis of paraffin-embedded mouse testis tissue labeling TATA binding protein TBP with ab220788 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Nuclear staining in mouse testis (PMID: 17570761; PMID: 11861477) is observed. Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.
Heat mediated antigen retrieval was performed with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemical analysis of paraffin-embedded rat testis tissue labeling TATA binding protein TBP with ab220788 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Nuclear staining in rat testis (PMID: 17570761; PMID: 11861477) is observed. Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.
Heat mediated antigen retrieval was performed with citrate buffer pH 6 before commencing with IHC staining protocol.
TATA binding protein TBP Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining using rabbit Anti-TATA binding protein TBP antibody
Immunohistochemical analysis of paraffin-embedded human bladder cancer tissue labeling TATA binding protein TBP with ab220788 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Nuclear staining in human bladder cancer (PMID: 17570761; PMID: 11861477) is observed. Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.
Heat mediated antigen retrieval was performed with citrate buffer pH 6 before commencing with IHC staining protocol.
TATA binding protein TBP Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining using rabbit Anti-TATA binding protein TBP antibody
Immunohistochemical analysis of paraffin-embedded human testis tissue labeling TATA binding protein TBP with ab220788 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Nuclear staining in human testis (PMID: 17570761; PMID: 11861477) is observed. Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.
Heat mediated antigen retrieval was performed with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HCT 116 (human colorectal carcinoma cell line) cells labeling TATA binding protein TBP with ab220788 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing nuclear staining in HCT 116 cells. The nuclear counter stain is DAPI (blue).
Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889) at 1/200 dilution (red).
Secondary antibody only control: Used PBS instead of primary antibody, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution.
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling TATA binding protein TBP with ab220788 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing nuclear staining in HeLa cells. The nuclear counter stain is DAPI (blue).
Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889) at 1/200 dilution (red).
Secondary antibody only control: Used PBS instead of primary antibody, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution.
ChIC/CUT&RUN was performed using a pAG-MNAse at a final concentration of 700 ng/mL, 3 x 10^5 HeLa (Human cervix adenocarcinoma epithelial cell line) cells and 3µg of ab220788 [EPR21954]. The resulting DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 10 million reads. The negative IgG control Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730 is also shown.
The ChIP data was conducted on chromatin prepared from HeLa cells. Cells were fixed with 1% formaldehyde for 10 minutes. ChIP was performed with 30 µg of chromatin and 4 µg of ab220788 with ChIP-Kit Transcription Factors ChIP-Seq (ChIP Kit (Transcription factors, ChIP-seq) ab270813). ChIP DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 30 million reads.
Additional screenshots of mapped reads can be found in the Protocol booklet in the Support and downloads section.
The University of Geneva owns patents relevant to ChIC (Chromatin Immuno-Cleavage) methods.
TATA binding protein TBP Flow Cytometry (Intracellular) staining using rabbit Anti-TATA binding protein TBP antibody
Intracellular flow cytometric analysis of4% paraformaldehyde-fixed, 90% methanol-permeabilized HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling TATA binding protein TBP with ab220788 at 1/500 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (black) and an unlabeled control (cells incubated with secondary antibody only) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077), at 1/2000 dilution was used as the secondary antibody.
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