AB74222

Anti-TATA binding protein TBP antibody - Loading Control

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(3 Publications)

Rabbit Polyclonal TATA binding protein TBP antibody. Suitable for WB and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human TBP.

View Alternative Names

GTF2D1, TF2D, TFIID, TBP, TATA-box-binding protein, TATA sequence-binding protein, TATA-binding factor, TATA-box factor, Transcription initiation factor TFIID TBP subunit

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Western blot - Anti-TATA binding protein TBP antibody - Loading Control (AB74222)

All lanes:

Western blot - Anti-TATA binding protein TBP antibody - Loading Control (ab74222) at 1 µg/mL

Lane 1:

HeLa whole cell lysate at 50 µg

Lane 2:

HeLa whole cell lysate at 15 µg

Lane 3:

HeLa whole cell lysate at 5 µg

Lane 4:

293T whole cell lysate at 50 µg

Predicted band size: 38 kDa

Observed band size: 45 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

Synthetic Peptide within Human TBP. The exact immunogen used to generate this antibody is proprietary information.

P20226

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Reactivity data

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: PBS, 1.815% Tris, 1.764% Sodium citrate

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle|Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The TATA binding protein (TBP) also known as TBP or TFIID (Transcription Factor IID) is a central player in transcription initiation. This protein weighing approximately 38 kDa binds to the TATA box a DNA sequence found in the promoter region of many genes especially those transcribed by RNA polymerase II. TBP facilitates the loading of the transcription machinery onto DNA enabling the start of transcription. It is found inside the nucleus of eukaryotic cells present across diverse cell types owing to its role in gene expression.

Biological function summary

TBP serves as a pivotal anchor in the assembly of the transcription preinitiation complex contributing to the process by bending DNA to assist other transcription factors such as TFIIA and TFIIB in binding. Its role in this complex illustrates its importance in regulating gene expression at a fundamental level. TBP’s interaction with other transcription factors ensures precise transcription regulation reflecting its significant contribution to cellular activities.

Pathways

TBP acts within the RNA polymerase II transcription initiation pathway and is integral in regulating gene expression. TBP's involvement is critical for the process of starting the transcription of mRNA. In its pathway TBP interacts with proteins like TFIID helping to anchor other transcription factors in the complex. By collaborating with these proteins TBP shapes gene regulatory networks essential for a wide range of cellular functions.

TBP is linked to conditions such as spinocerebellar ataxia type 17 (SCA17) and Huntington's disease. SCA17 is associated with mutations in the TBP gene that disrupt its usual functioning leading to neurodegeneration. In Huntington's disease TBP's interaction with proteins such as huntingtin may influence nuclear signaling pathways involved in disease progression. The understanding of TBP’s role in these disorders underlines its relevance in research focused on genetic diseases and transcription-related pathologies.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

The TFIID basal transcription factor complex plays a major role in the initiation of RNA polymerase II (Pol II)-dependent transcription (PubMed : 33795473). TFIID recognizes and binds promoters with or without a TATA box via its subunit TBP, a TATA-box-binding protein, and promotes assembly of the pre-initiation complex (PIC) (PubMed : 2194289, PubMed : 2363050, PubMed : 2374612, PubMed : 27193682, PubMed : 33795473). The TFIID complex consists of TBP and TBP-associated factors (TAFs), including TAF1, TAF2, TAF3, TAF4, TAF5, TAF6, TAF7, TAF8, TAF9, TAF10, TAF11, TAF12 and TAF13 (PubMed : 27007846, PubMed : 33795473). The TFIID complex structure can be divided into 3 modules TFIID-A, TFIID-B, and TFIID-C (PubMed : 33795473). TBP forms the TFIID-A module together with TAF3 and TAF5 (PubMed : 33795473). TBP is a general transcription factor that functions at the core of the TFIID complex (PubMed : 2194289, PubMed : 2363050, PubMed : 2374612, PubMed : 27193682, PubMed : 33795473, PubMed : 9836642). During assembly of the core PIC on the promoter, as part of TFIID, TBP binds to and also bends promoter DNA, irrespective of whether the promoter contains a TATA box (PubMed : 33795473). Component of a BRF2-containing transcription factor complex that regulates transcription mediated by RNA polymerase III (PubMed : 26638071). Component of the transcription factor SL1/TIF-IB complex, which is involved in the assembly of the PIC during RNA polymerase I-dependent transcription (PubMed : 15970593). The rate of PIC formation probably is primarily dependent on the rate of association of SL1 with the rDNA promoter (PubMed : 15970593). SL1 is involved in stabilization of nucleolar transcription factor 1/UBTF on rDNA (PubMed : 15970593).

See full target information TBP

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Biomedical papers of the Medical Faculty of the University Palacky, Olomouc, Czechoslovakia : PubMed40709542

2025

Potential of silymarin and its polyphenols to affect Nrf2 signalling pathway in human skin cells.

Applications

Unspecified application

Species

Unspecified reactive species

Alena Rysava,Katerina Valentova,Katerina Cizkova,Jitka Vostalova,Bohumil Zalesak,Alena Rajnochova Svobodova

PLoS pathogens 21:e1013184 PubMed40367285

2025

Virus and cell specific HMGB1 secretion and subepithelial infiltrate formation in adenovirus keratitis.

Applications

Unspecified application

Species

Unspecified reactive species

Amrita Saha,Mohammad Mirazul Islam,Rahul Kumar,Ashrafali Mohamed Ismail,Emanuel Garcia,Rama R Gullapali,James Chodosh,Jaya Rajaiya

American journal of hematology 91:1227-1233 PubMed27622320

2016

Clonal hematopoiesis in patients with dyskeratosis congenita.

Applications

Unspecified application

Species

Unspecified reactive species

Nieves Perdigones,Juan C Perin,Irene Schiano,Peter Nicholas,Jaclyn A Biegel,Philip J Mason,Daria V Babushok,Monica Bessler

View all publications

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Product promise

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Anti-TATA binding protein TBP antibody - Loading Control

Western blot - Anti-TATA binding protein TBP antibody - Loading Control (AB74222)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

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