Anti-TATA binding protein TBP antibody [mAbcam51841] is a Mouse Monoclonal antibody that is used in TATA binding protein TBP ChIP, IHC-P, IP, Western Blot. Suitable for Human, Mouse, Rat samples.
The TATA-binding protein (TBP) is a crucial transcription factor that binds to the TATA box, a specific DNA sequence found in the promoter region of many genes. By binding to the TATA box, TBP helps recruit RNA polymerase II and other transcription factors, facilitating the initiation of gene transcription. This process is essential for regulating gene expression and ensuring the proper production of proteins necessary for various cellular functions.
Images
![Western blot - Anti-TATA binding protein TBP antibody [mAbcam51841] (AB300656), expandable thumbnail](https://content.abcam.com/products/images/tata-binding-protein-tbp-antibody-mabcam51841-ab300656--western-blot-img401949.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-TATA binding protein TBP antibody [mAbcam51841] (AB300656)")
![Immunohistochemistry - Anti-TATA binding protein TBP antibody [mAbcam51841] (AB300656), expandable thumbnail](https://content.abcam.com/products/images/tata-binding-protein-tbp-antibody-mabcam51841-ab300656--immunohistochemistry-img401083.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry - Anti-TATA binding protein TBP antibody [mAbcam51841] (AB300656)")
![Immunohistochemistry - Anti-TATA binding protein TBP antibody [mAbcam51841] (AB300656), expandable thumbnail](https://content.abcam.com/products/images/tata-binding-protein-tbp-antibody-mabcam51841-ab300656--immunohistochemistry-img401082.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry - Anti-TATA binding protein TBP antibody [mAbcam51841] (AB300656)")
![Immunohistochemistry - Anti-TATA binding protein TBP antibody [mAbcam51841] (AB300656), expandable thumbnail](https://content.abcam.com/products/images/tata-binding-protein-tbp-antibody-mabcam51841-ab300656--immunohistochemistry-img401081.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry - Anti-TATA binding protein TBP antibody [mAbcam51841] (AB300656)")
![Immunohistochemistry - Anti-TATA binding protein TBP antibody [mAbcam51841] (AB300656), expandable thumbnail](https://content.abcam.com/products/images/tata-binding-protein-tbp-antibody-mabcam51841-ab300656--immunohistochemistry-img401080.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry - Anti-TATA binding protein TBP antibody [mAbcam51841] (AB300656)")
Key facts
- Isotype
- IgG2c
- Host species
- Mouse
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| ChIP | IP | Flow Cyt (Intra) | ICC | IHC-P | WB | |
|---|---|---|---|---|---|---|
| Human | Tested | Tested | Not recommended | Not recommended | Tested | Tested |
| Mouse | Expected | Tested | Not recommended | Not recommended | Tested | Tested |
| Rat | Expected | Expected | Not recommended | Not recommended | Tested | Tested |
ChIP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 5 µg/mL | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/30 | Notes - |
Species Human | Dilution info 1/30 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Flow Cyt (Intra)
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human, Rat | Dilution info - | Notes - |
ICC
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info 1/10000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Species Mouse | Dilution info 1/10000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Species Human | Dilution info 1/10000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info 1/1000 | Notes - |
Species Mouse | Dilution info 1/1000 | Notes - |
Species Human | Dilution info 1/1000 | Notes - |
Target data
Function
The TFIID basal transcription factor complex plays a major role in the initiation of RNA polymerase II (Pol II)-dependent transcription (PubMed:33795473). TFIID recognizes and binds promoters with or without a TATA box via its subunit TBP, a TATA-box-binding protein, and promotes assembly of the pre-initiation complex (PIC) (PubMed:2194289, PubMed:2363050, PubMed:2374612, PubMed:27193682, PubMed:33795473). The TFIID complex consists of TBP and TBP-associated factors (TAFs), including TAF1, TAF2, TAF3, TAF4, TAF5, TAF6, TAF7, TAF8, TAF9, TAF10, TAF11, TAF12 and TAF13 (PubMed:27007846, PubMed:33795473). The TFIID complex structure can be divided into 3 modules TFIID-A, TFIID-B, and TFIID-C (PubMed:33795473). TBP forms the TFIID-A module together with TAF3 and TAF5 (PubMed:33795473). TBP is a general transcription factor that functions at the core of the TFIID complex (PubMed:2194289, PubMed:2363050, PubMed:2374612, PubMed:27193682, PubMed:33795473, PubMed:9836642). During assembly of the core PIC on the promoter, as part of TFIID, TBP binds to and also bends promoter DNA, irrespective of whether the promoter contains a TATA box (PubMed:33795473). Component of a BRF2-containing transcription factor complex that regulates transcription mediated by RNA polymerase III (PubMed:26638071). Component of the transcription factor SL1/TIF-IB complex, which is involved in the assembly of the PIC during RNA polymerase I-dependent transcription (PubMed:15970593). The rate of PIC formation probably is primarily dependent on the rate of association of SL1 with the rDNA promoter (PubMed:15970593). SL1 is involved in stabilization of nucleolar transcription factor 1/UBTF on rDNA (PubMed:15970593).
Alternative names
GTF2D1, TF2D, TFIID, TBP, TATA-box-binding protein, TATA sequence-binding protein, TATA-binding factor, TATA-box factor, Transcription initiation factor TFIID TBP subunit
Recommended products
Anti-TATA binding protein TBP antibody [mAbcam51841] is a Mouse Monoclonal antibody that is used in TATA binding protein TBP ChIP, IHC-P, IP, Western Blot. Suitable for Human, Mouse, Rat samples.
The TATA-binding protein (TBP) is a crucial transcription factor that binds to the TATA box, a specific DNA sequence found in the promoter region of many genes. By binding to the TATA box, TBP helps recruit RNA polymerase II and other transcription factors, facilitating the initiation of gene transcription. This process is essential for regulating gene expression and ensuring the proper production of proteins necessary for various cellular functions.
Key facts
- Isotype
- IgG2c
- Host species
- Mouse
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- mAbcam51841
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The TATA binding protein (TBP) also known as TBP or TFIID (Transcription Factor IID) is a central player in transcription initiation. This protein weighing approximately 38 kDa binds to the TATA box a DNA sequence found in the promoter region of many genes especially those transcribed by RNA polymerase II. TBP facilitates the loading of the transcription machinery onto DNA enabling the start of transcription. It is found inside the nucleus of eukaryotic cells present across diverse cell types owing to its role in gene expression.
- Biological function summary
TBP serves as a pivotal anchor in the assembly of the transcription preinitiation complex contributing to the process by bending DNA to assist other transcription factors such as TFIIA and TFIIB in binding. Its role in this complex illustrates its importance in regulating gene expression at a fundamental level. TBP’s interaction with other transcription factors ensures precise transcription regulation reflecting its significant contribution to cellular activities.
- Pathways
TBP acts within the RNA polymerase II transcription initiation pathway and is integral in regulating gene expression. TBP's involvement is critical for the process of starting the transcription of mRNA. In its pathway TBP interacts with proteins like TFIID helping to anchor other transcription factors in the complex. By collaborating with these proteins TBP shapes gene regulatory networks essential for a wide range of cellular functions.
- Associated diseases and disorders
TBP is linked to conditions such as spinocerebellar ataxia type 17 (SCA17) and Huntington's disease. SCA17 is associated with mutations in the TBP gene that disrupt its usual functioning leading to neurodegeneration. In Huntington's disease TBP's interaction with proteins such as huntingtin may influence nuclear signaling pathways involved in disease progression. The understanding of TBP’s role in these disorders underlines its relevance in research focused on genetic diseases and transcription-related pathologies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
5 product images
![Western blot - Anti-TATA binding protein TBP antibody [mAbcam51841] (ab300656), expandable thumbnail](https://content.abcam.com/products/images/tata-binding-protein-tbp-antibody-mabcam51841-ab300656--western-blot-img401949.jpg "Western blot - Anti-TATA binding protein TBP antibody [mAbcam51841] (ab300656)")
Western blot - Anti-TATA binding protein TBP antibody [mAbcam51841] (ab300656)
All lanes: Western blot - Anti-TATA binding protein TBP antibody [mAbcam51841] (ab300656) at 1/1000 dilution
Lane 1: HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate at 20 µg
Lane 2: 293T (human embryonic kidney epithelial cell) whole cell lysate at 20 µg
Lane 3: C2C12 (mouse myoblasts myoblast) whole cell lysate at 20 µg
Lane 4: NIH/3T3 (mouse embryonic fibroblast) whole cell lysate at 20 µg
Lane 5: C6 (rat glial tumor glial cell) whole cell lysate at 20 µg
Lane 6: PC-12 (rat adrenal gland pheochromocytoma ) whole cell lysate at 20 µg
Lane 7: Mouse testis tissue lysate at 20 µg
Lane 8: Rat testis tissue lysate at 20 µg
Lane 9: Human testis tissue lysate at 20 µg
SecondaryAll lanes: Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution
Observed band size: 40 kDa, 35 kDa
![Immunohistochemistry - Anti-TATA binding protein TBP antibody [mAbcam51841] (ab300656), expandable thumbnail](https://content.abcam.com/products/images/tata-binding-protein-tbp-antibody-mabcam51841-ab300656--immunohistochemistry-img401083.jpg "Immunohistochemistry - Anti-TATA binding protein TBP antibody [mAbcam51841] (ab300656)")
Immunohistochemistry - Anti-TATA binding protein TBP antibody [mAbcam51841] (ab300656)
Immunohistochemical analysis of paraffin-embedded Rat colon tissue labeling TATA binding protein TBP with ab300656 at 1/10000 (0.103 ug/ml) followed by a LeicaDS9800 (Bond Polymer Refine Detection) was used at Ready to use dilution. Nuclear staining on rat colon. The section was incubated with ab51841 for 10 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control: Secondary antibody is LeicaDS9800 (Bond Polymer Refine Detection) was used at Ready to use dilution. Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins
![Immunohistochemistry - Anti-TATA binding protein TBP antibody [mAbcam51841] (ab300656), expandable thumbnail](https://content.abcam.com/products/images/tata-binding-protein-tbp-antibody-mabcam51841-ab300656--immunohistochemistry-img401082.jpg "Immunohistochemistry - Anti-TATA binding protein TBP antibody [mAbcam51841] (ab300656)")
Immunohistochemistry - Anti-TATA binding protein TBP antibody [mAbcam51841] (ab300656)
Immunohistochemical analysis of paraffin-embedded Mouse stomach tissue labeling TATA binding protein TBP with ab300656 at 1/10000 (0.103 ug/ml) followed by a LeicaDS9800 (Bond Polymer Refine Detection) was used at Ready to use dilution. Nuclear staining on mouse stomach. The section was incubated with ab51841 for 10 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control: Secondary antibody is LeicaDS9800 (Bond Polymer Refine Detection) was used at Ready to use dilution. Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins
![Immunohistochemistry - Anti-TATA binding protein TBP antibody [mAbcam51841] (ab300656), expandable thumbnail](https://content.abcam.com/products/images/tata-binding-protein-tbp-antibody-mabcam51841-ab300656--immunohistochemistry-img401081.jpg "Immunohistochemistry - Anti-TATA binding protein TBP antibody [mAbcam51841] (ab300656)")
Immunohistochemistry - Anti-TATA binding protein TBP antibody [mAbcam51841] (ab300656)
Immunohistochemical analysis of paraffin-embedded Human colon carcinom tissue labeling TATA binding protein TBP with ab300656 at 1/10000 (0.103 ug/ml) followed by a LeicaDS9800 (Bond Polymer Refine Detection) was used at Ready to use dilution. Nuclear staining on human colon carcinoma. The section was incubated with ab51841 for 10 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control: Secondary antibody is LeicaDS9800 (Bond Polymer Refine Detection) was used at Ready to use dilution. Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins
![Immunohistochemistry - Anti-TATA binding protein TBP antibody [mAbcam51841] (ab300656), expandable thumbnail](https://content.abcam.com/products/images/tata-binding-protein-tbp-antibody-mabcam51841-ab300656--immunohistochemistry-img401080.jpg "Immunohistochemistry - Anti-TATA binding protein TBP antibody [mAbcam51841] (ab300656)")
Immunohistochemistry - Anti-TATA binding protein TBP antibody [mAbcam51841] (ab300656)
Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling TATA binding protein TBP with ab300656 at 1/10000 (0.103 ug/ml) followed by a LeicaDS9800 (Bond Polymer Refine Detection) was used at Ready to use dilution. Nuclear staining on human colon. The section was incubated with ab51841 for 10 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control: Secondary antibody is LeicaDS9800 (Bond Polymer Refine Detection) was used at Ready to use dilution. Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins
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