Anti-TATA binding protein TBP antibody [mAbcam51841] (BSA and Azide free)

• IHC

Supplier Data

Immunohistochemistry - Anti-TATA binding protein TBP antibody [mAbcam51841] (BSA and Azide free) (AB282715)

This data was developed using ab300656, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human colon carcinom tissue labeling TATA binding protein TBP with ab300656 at 1/10000 (0.103 ug/ml) followed by a LeicaDS9800 (Bond™ Polymer Refine Detection) was used at Ready to use dilution. Nuclear staining on human colon carcinoma. The section was incubated with ab51841 for 10 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is LeicaDS9800 (Bond™ Polymer Refine Detection) was used at Ready to use dilution. Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins

• IHC

Supplier Data

Immunohistochemistry - Anti-TATA binding protein TBP antibody [mAbcam51841] (BSA and Azide free) (AB282715)

This data was developed using ab300656, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling TATA binding protein TBP with ab300656 at 1/10000 (0.103 ug/ml) followed by a LeicaDS9800 (Bond™ Polymer Refine Detection) was used at Ready to use dilution. Nuclear staining on human colon. The section was incubated with ab51841 for 10 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is LeicaDS9800 (Bond® Polymer Refine Detection) was used at Ready to use dilution. Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins

• IHC

Supplier Data

Immunohistochemistry - Anti-TATA binding protein TBP antibody [mAbcam51841] (BSA and Azide free) (AB282715)

This data was developed using ab300656, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Rat colon tissue labeling TATA binding protein TBP with ab300656 at 1/10000 (0.103 ug/ml) followed by a LeicaDS9800 (Bond™ Polymer Refine Detection) was used at Ready to use dilution. Nuclear staining on rat colon. The section was incubated with ab51841 for 10 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is LeicaDS9800 (Bond™ Polymer Refine Detection) was used at Ready to use dilution. Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins

• IHC

Supplier Data

Immunohistochemistry - Anti-TATA binding protein TBP antibody [mAbcam51841] (BSA and Azide free) (AB282715)

This data was developed using ab300656, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Mouse stomach tissue labeling TATA binding protein TBP with ab300656 at 1/10000 (0.103 ug/ml) followed by a LeicaDS9800 (Bond™ Polymer Refine Detection) was used at Ready to use dilution. Nuclear staining on mouse stomach. The section was incubated with ab51841 for 10 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is LeicaDS9800 (Bond™ Polymer Refine Detection) was used at Ready to use dilution. Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins

• WB

Supplier Data

Western blot - Anti-TATA binding protein TBP antibody [mAbcam51841] (BSA and Azide free) (AB282715)

All lanes:

Western blot - Anti-TATA binding protein TBP antibody [mAbcam51841] - Loading Control (<a href='/en-us/products/primary-antibodies/tata-binding-protein-tbp-antibody-mabcam51841-loading-control-ab300656'>ab300656</a>) at 1/1000 dilution

Lane 1:

HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 2:

293T (human embryonic kidney epithelial cell) whole cell lysate at 20 µg

Lane 3:

C2C12 (mouse myoblasts myoblast) whole cell lysate at 20 µg

Lane 4:

NIH/3T3 (mouse embryonic fibroblast) whole cell lysate at 20 µg

Lane 5:

C6 (rat glial tumor glial cell) whole cell lysate at 20 µg

Lane 6:

PC-12 (rat adrenal gland pheochromocytoma ) whole cell lysate at 20 µg

Lane 7:

Mouse testis tissue lysate at 20 µg

Lane 8:

Rat testis tissue lysate at 20 µg

Lane 9:

Human testis tissue lysate at 20 µg

Secondary

All lanes:

Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

Observed band size: 40 kDa,35 kDa

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