AB300656

Anti-TATA binding protein TBP antibody [mAbcam51841] - Loading Control

BOND RX™ Validated
Recombinant
Lab Essentials
What is this?

Be the first to review this product! Submit a review

(4 Publications)

Anti-TATA binding protein TBP antibody [mAbcam51841] is a Mouse Monoclonal antibody that is used in TATA binding protein TBP ChIP, IHC-P, IP, Western Blot. Suitable for Human, Mouse, Rat samples.

The TATA-binding protein (TBP) is a crucial transcription factor that binds to the TATA box, a specific DNA sequence found in the promoter region of many genes. By binding to the TATA box, TBP helps recruit RNA polymerase II and other transcription factors, facilitating the initiation of gene transcription. This process is essential for regulating gene expression and ensuring the proper production of proteins necessary for various cellular functions.

View Alternative Names

GTF2D1, TF2D, TFIID, TBP, TATA-box-binding protein, TATA sequence-binding protein, TATA-binding factor, TATA-box factor, Transcription initiation factor TFIID TBP subunit

7 Images

Supplier Data

Immunohistochemistry - Anti-TATA binding protein TBP antibody [mAbcam51841] - Loading Control (AB300656)

Immunohistochemical analysis of paraffin-embedded Human colon carcinom tissue labeling TATA binding protein TBP with ab300656 at 1/10000 (0.103 ug/ml) followed by a LeicaDS9800 (Bond Polymer Refine Detection) was used at Ready to use dilution. Nuclear staining on human colon carcinoma. The section was incubated with ab51841 for 10 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is LeicaDS9800 (Bond Polymer Refine Detection) was used at Ready to use dilution. Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins

Supplier Data

Immunohistochemistry - Anti-TATA binding protein TBP antibody [mAbcam51841] - Loading Control (AB300656)

Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling TATA binding protein TBP with ab300656 at 1/10000 (0.103 ug/ml) followed by a LeicaDS9800 (Bond Polymer Refine Detection) was used at Ready to use dilution. Nuclear staining on human colon. The section was incubated with ab51841 for 10 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is LeicaDS9800 (Bond Polymer Refine Detection) was used at Ready to use dilution. Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins

Lab

Immunoprecipitation - Anti-TATA binding protein TBP antibody [mAbcam51841] - Loading Control (AB300656)

TATA binding protein TBP was immunoprecipitated from 0.35 mg HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate (10 µg) with ab300656 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab300656 at 1/2000 dilution. Mouse IgG for IP (HRP) (ab131368) was used as the secondary at 1/5000 dilution.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

All lanes:

Immunoprecipitation - Anti-TATA binding protein TBP antibody [mAbcam51841] - Loading Control (ab300656) at 1/30 dilution

Lane 1:

HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate at 10 µg

Lane 2:

HeLa whole cell lysate

Secondary

All lanes:

Immunoprecipitation - Anti-mouse IgG for IP (HRP) (<a href='/en-us/products/secondary-antibodies/mouse-igg-for-ip-hrp-ab131368'>ab131368</a>) at 1/5000 dilution

Observed band size: 35 kDa,38 kDa

false

Exposure time: 24s

Supplier Data

Immunohistochemistry - Anti-TATA binding protein TBP antibody [mAbcam51841] - Loading Control (AB300656)

Immunohistochemical analysis of paraffin-embedded Mouse stomach tissue labeling TATA binding protein TBP with ab300656 at 1/10000 (0.103 ug/ml) followed by a LeicaDS9800 (Bond Polymer Refine Detection) was used at Ready to use dilution. Nuclear staining on mouse stomach. The section was incubated with ab51841 for 10 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is LeicaDS9800 (Bond Polymer Refine Detection) was used at Ready to use dilution. Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins

Supplier Data

Immunohistochemistry - Anti-TATA binding protein TBP antibody [mAbcam51841] - Loading Control (AB300656)

Immunohistochemical analysis of paraffin-embedded Rat colon tissue labeling TATA binding protein TBP with ab300656 at 1/10000 (0.103 ug/ml) followed by a LeicaDS9800 (Bond Polymer Refine Detection) was used at Ready to use dilution. Nuclear staining on rat colon. The section was incubated with ab51841 for 10 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is LeicaDS9800 (Bond Polymer Refine Detection) was used at Ready to use dilution. Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins

Lab

Immunoprecipitation - Anti-TATA binding protein TBP antibody [mAbcam51841] - Loading Control (AB300656)

TATA binding protein TBP was immunoprecipitated from 0.35 mg NIH/3T3 (mouse embryonic fibroblast) whole cell lysate (10 µg) with ab300656 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab300656 at 1/2000 dilution. Mouse IgG for IP (HRP) (ab131368) was used as the secondary at 1/5000 dilution.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

All lanes:

Immunoprecipitation - Anti-TATA binding protein TBP antibody [mAbcam51841] - Loading Control (ab300656) at 1/30 dilution

Lane 1:

NIH/3T3 (mouse embryonic fibroblast) whole cell lysate at 10 µg

Lane 2:

NIH/3T3 whole cell lysate

Secondary

All lanes:

Immunoprecipitation - Anti-mouse IgG for IP (HRP) (<a href='/en-us/products/secondary-antibodies/mouse-igg-for-ip-hrp-ab131368'>ab131368</a>) at 1/5000 dilution

Predicted band size: 38 kDa

Observed band size: 36 kDa

false

Exposure time: 24s

Supplier Data

Western blot - Anti-TATA binding protein TBP antibody [mAbcam51841] - Loading Control (AB300656)

All lanes:

Western blot - Anti-TATA binding protein TBP antibody [mAbcam51841] - Loading Control (ab300656) at 1/1000 dilution

Lane 1:

HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 2:

293T (human embryonic kidney epithelial cell) whole cell lysate at 20 µg

Lane 3:

C2C12 (mouse myoblasts myoblast) whole cell lysate at 20 µg

Lane 4:

NIH/3T3 (mouse embryonic fibroblast) whole cell lysate at 20 µg

Lane 5:

C6 (rat glial tumor glial cell) whole cell lysate at 20 µg

Lane 6:

PC-12 (rat adrenal gland pheochromocytoma ) whole cell lysate at 20 µg

Lane 7:

Mouse testis tissue lysate at 20 µg

Lane 8:

Rat testis tissue lysate at 20 µg

Lane 9:

Human testis tissue lysate at 20 µg

Secondary

All lanes:

Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

Observed band size: 40 kDa,35 kDa

false

Carrier free

Anti-TATA binding protein TBP antibody [mAbcam51841] (BSA and Azide free)

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

mAbcam51841

Isotype

IgG2c

Carrier free

Reacts with

Rat, Mouse, Human

Applications

ChIP, WB, IHC-P, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style

left-column

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ChIP" : {"fullname" : "ChIP", "shortname":"ChIP"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ChIP-species-checked": "testedAndGuaranteed", "ChIP-species-dilution-info": "5 µg/mL", "ChIP-species-notes": "", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/30", "IP-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "ICC-species-checked": "notRecommended", "ICC-species-dilution-info": "", "ICC-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/10000", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "" }, "Mouse": { "ChIP-species-checked": "guaranteed", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/30", "IP-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "ICC-species-checked": "notRecommended", "ICC-species-dilution-info": "", "ICC-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/10000", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "" }, "Rat": { "ChIP-species-checked": "guaranteed", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "ICC-species-checked": "notRecommended", "ICC-species-dilution-info": "", "ICC-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/10000", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "" } } }

style

left-column

Product details

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The TATA binding protein (TBP) also known as TBP or TFIID (Transcription Factor IID) is a central player in transcription initiation. This protein weighing approximately 38 kDa binds to the TATA box a DNA sequence found in the promoter region of many genes especially those transcribed by RNA polymerase II. TBP facilitates the loading of the transcription machinery onto DNA enabling the start of transcription. It is found inside the nucleus of eukaryotic cells present across diverse cell types owing to its role in gene expression.

Biological function summary

TBP serves as a pivotal anchor in the assembly of the transcription preinitiation complex contributing to the process by bending DNA to assist other transcription factors such as TFIIA and TFIIB in binding. Its role in this complex illustrates its importance in regulating gene expression at a fundamental level. TBP’s interaction with other transcription factors ensures precise transcription regulation reflecting its significant contribution to cellular activities.

Pathways

TBP acts within the RNA polymerase II transcription initiation pathway and is integral in regulating gene expression. TBP's involvement is critical for the process of starting the transcription of mRNA. In its pathway TBP interacts with proteins like TFIID helping to anchor other transcription factors in the complex. By collaborating with these proteins TBP shapes gene regulatory networks essential for a wide range of cellular functions.

TBP is linked to conditions such as spinocerebellar ataxia type 17 (SCA17) and Huntington's disease. SCA17 is associated with mutations in the TBP gene that disrupt its usual functioning leading to neurodegeneration. In Huntington's disease TBP's interaction with proteins such as huntingtin may influence nuclear signaling pathways involved in disease progression. The understanding of TBP’s role in these disorders underlines its relevance in research focused on genetic diseases and transcription-related pathologies.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

The TFIID basal transcription factor complex plays a major role in the initiation of RNA polymerase II (Pol II)-dependent transcription (PubMed : 33795473). TFIID recognizes and binds promoters with or without a TATA box via its subunit TBP, a TATA-box-binding protein, and promotes assembly of the pre-initiation complex (PIC) (PubMed : 2194289, PubMed : 2363050, PubMed : 2374612, PubMed : 27193682, PubMed : 33795473). The TFIID complex consists of TBP and TBP-associated factors (TAFs), including TAF1, TAF2, TAF3, TAF4, TAF5, TAF6, TAF7, TAF8, TAF9, TAF10, TAF11, TAF12 and TAF13 (PubMed : 27007846, PubMed : 33795473). The TFIID complex structure can be divided into 3 modules TFIID-A, TFIID-B, and TFIID-C (PubMed : 33795473). TBP forms the TFIID-A module together with TAF3 and TAF5 (PubMed : 33795473). TBP is a general transcription factor that functions at the core of the TFIID complex (PubMed : 2194289, PubMed : 2363050, PubMed : 2374612, PubMed : 27193682, PubMed : 33795473, PubMed : 9836642). During assembly of the core PIC on the promoter, as part of TFIID, TBP binds to and also bends promoter DNA, irrespective of whether the promoter contains a TATA box (PubMed : 33795473). Component of a BRF2-containing transcription factor complex that regulates transcription mediated by RNA polymerase III (PubMed : 26638071). Component of the transcription factor SL1/TIF-IB complex, which is involved in the assembly of the PIC during RNA polymerase I-dependent transcription (PubMed : 15970593). The rate of PIC formation probably is primarily dependent on the rate of association of SL1 with the rDNA promoter (PubMed : 15970593). SL1 is involved in stabilization of nucleolar transcription factor 1/UBTF on rDNA (PubMed : 15970593).

See full target information TBP

style

left-column

Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Cancer drug resistance (Alhambra, Calif.) 8:26 PubMed40521302

2025

PER2 expression and cellular localization play a critical role in tumor aggressiveness and drug resistance in an model of hepatocellular carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Mariarosaria Negri,Feliciana Amatrudo,Donatella Paola Provvisiero,Roberta Patalano,Giovanna Trinchese,Fabiano Cimmino,Cristina de Angelis,Chiara Simeoli,Renata Simona Auriemma,Maria Pina Mollica,Annamaria Colao,Rosario Pivonello,Claudia Pivonello

Redox biology 71:103117 PubMed38479223

2024

Inhibition of glycogen synthase kinase-3 enhances NRF2 protein stability, nuclear localisation and target gene transcription in pancreatic beta cells.

Applications

Unspecified application

Species

Unspecified reactive species

Chinmai Patibandla,Lidy van Aalten,Albena T Dinkova-Kostova,Tadashi Honda,Antonio Cuadrado,Raquel Fernández-Ginés,Alison D McNeilly,John D Hayes,James Cantley,Calum Sutherland

Journal of cardiovascular development and disease 11: PubMed38248875

2024

Calciprotein Particles Induce Cellular Compartment-Specific Proteome Alterations in Human Arterial Endothelial Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Daria Shishkova,Arseniy Lobov,Egor Repkin,Victoria Markova,Yulia Markova,Anna Sinitskaya,Maxim Sinitsky,Egor Kondratiev,Evgenia Torgunakova,Anton Kutikhin

iScience 26:108515 PubMed38089580

2023

Cyclophilin A facilitates influenza B virus replication by stabilizing viral proteins.

Applications

Unspecified application

Species

Unspecified reactive species

Huizi Li,Wenhui Fan,Jie Min,Xiaoyuan Bai,Wenxian Yang,Heqiao Li,Yuna Zhao,Runshan Lin,Xiaojuan Jia,Wenjun Liu,Lei Sun

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-TATA binding protein TBP antibody [mAbcam51841] - Loading Control

Immunohistochemistry - Anti-TATA binding protein TBP antibody [mAbcam51841] - Loading Control (AB300656)

Immunohistochemistry - Anti-TATA binding protein TBP antibody [mAbcam51841] - Loading Control (AB300656)

Immunoprecipitation - Anti-TATA binding protein TBP antibody [mAbcam51841] - Loading Control (AB300656)

Immunohistochemistry - Anti-TATA binding protein TBP antibody [mAbcam51841] - Loading Control (AB300656)

Immunohistochemistry - Anti-TATA binding protein TBP antibody [mAbcam51841] - Loading Control (AB300656)

Immunoprecipitation - Anti-TATA binding protein TBP antibody [mAbcam51841] - Loading Control (AB300656)

Western blot - Anti-TATA binding protein TBP antibody [mAbcam51841] - Loading Control (AB300656)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Reagents, Controls & Accessories

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

Product promise