Skip to main content

Rabbit Recombinant Monoclonal TAU antibody. Suitable for WB, ICC/IF, Flow Cyt (Intra) and reacts with Mouse, Rat, Human samples. Cited in 22 publications.


Images

Western blot - Anti-Tau antibody [EP2456Y] (AB76128), expandable thumbnail
  • Western blot - Anti-Tau antibody [EP2456Y] (AB76128), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-Tau antibody [EP2456Y] (AB76128), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-Tau antibody [EP2456Y] (AB76128), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-Tau antibody [EP2456Y] (AB76128), expandable thumbnail

Publications

  • Cellular and molecular life sciences : CMLS 80:3782023
    Mistranslation-associated perturbations of proteostasis do not promote accumulation of amyloid beta and plaque deposition in aged mouse brain.
    Applications:
    Unspecified application
    Reactive species:
    Unspecified reactive species
    Harshitha Santhosh Kumar,James Moore,Adrian C Steiner,Emmanuel Sotirakis,Benjamin Schärli,Patricia Isnard-Petit,Kader Thiam,David P Wolfer,Erik C Böttger
    PubMed 38010524
  • JCI insight 8:2023
    N6-methyladenosine-mediated overexpression of long noncoding RNA ADAMTS9-AS2 triggers neuroblastoma differentiation via regulating LIN28B/let-7/MYCN signaling.
    Applications:
    Unspecified application
    Reactive species:
    Unspecified reactive species
    Yun Liu,Jun Zhang,Fang Cao,Xiaobao Dong,Jie Li,Yanna Cao,Zhanglin Li,Yan Guo,Jie Yan,Yuanyuan Liu,Qiang Zhao
    PubMed 37991019

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPWBICC/IFFlow Cyt (Intra)
Human
Not recommended
Tested
Tested
Tested
Mouse
Not recommended
Tested
Tested
Tested
Rat
Not recommended
Tested
Tested
Tested

Not recommended
Not recommended

Species

Mouse, Rat, Human

Dilution info

-

Notes

-

Tested
Tested

Species

Mouse

Dilution info

1/10000

Notes

For unpurified use at 1/2000 - 1/10000.

Species

Rat

Dilution info

1/10000

Notes

For unpurified use at 1/2000 - 1/10000.

Species

Human

Dilution info

1/10000

Notes

For unpurified use at 1/2000 - 1/10000.

Tested
Tested

Species

Mouse

Dilution info

1/50

Notes

For unpurified use at 1/100 - 1/500.

Species

Rat

Dilution info

1/50

Notes

For unpurified use at 1/100 - 1/500.

Species

Human

Dilution info

1/50

Notes

For unpurified use at 1/100 - 1/500.

Tested
Tested

Species

Mouse

Dilution info

1/500

Notes

For unpurified use at 1/160 - 1/1000. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Species

Rat

Dilution info

1/500

Notes

For unpurified use at 1/160 - 1/1000. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Species

Human

Dilution info

1/500

Notes

For unpurified use at 1/160 - 1/1000. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Associated Products

Select an associated product type

8 products for Alternative Product

    4 products for Alternative Version

      Target data

      Function

      Promotes microtubule assembly and stability, and might be involved in the establishment and maintenance of neuronal polarity (PubMed:21985311). The C-terminus binds axonal microtubules while the N-terminus binds neural plasma membrane components, suggesting that tau functions as a linker protein between both (PubMed:21985311, PubMed:32961270). Axonal polarity is predetermined by TAU/MAPT localization (in the neuronal cell) in the domain of the cell body defined by the centrosome. The short isoforms allow plasticity of the cytoskeleton whereas the longer isoforms may preferentially play a role in its stabilization.

      Alternative names

      Microtubule-associated protein tau, Neurofibrillary tangle protein, Paired helical filament-tau, PHF-tau, MAPT, MTBT1, TAU, MAPTL

      Recommended products

      1. Loading...
      2. Loading...
      3. Loading...
      4. Loading...

      Rabbit Recombinant Monoclonal TAU antibody. Suitable for WB, ICC/IF, Flow Cyt (Intra) and reacts with Mouse, Rat, Human samples. Cited in 22 publications.

      Alternative names

      Microtubule-associated protein tau, Neurofibrillary tangle protein, Paired helical filament-tau, PHF-tau, MAPT, MTBT1, TAU, MAPTL

      Key facts

      Isotype

      IgG

      Form

      Liquid

      Clonality

      Monoclonal

      Immunogen
      • The exact immunogen used to generate this antibody is proprietary information.
      Clone number

      EP2456Y

      Purification technique

      Affinity purification Protein A

      Specificity

      This antibody detects Tau, whether phosphorylated or unphosphorylated on serine 635.
      The specificity of this antibody refers to P10636-8.
      This antibody clone has been shown to react with Tau 4R and 3R isoforms (doi.org/10.1101/2023.04.13.536711).
      Our testing suggests that this antibody clone does not cross-reacts with MAP2 or MAP4

      Dissociation constant

      1.55 x 10-11 M

      Concentration
      Loading...

      Storage

      Shipped at conditions

      Blue Ice

      Appropriate short-term storage duration

      1-2 weeks

      Appropriate short-term storage conditions

      +4°C

      Appropriate long-term storage conditions

      -20°C

      Aliquoting information

      Upon delivery aliquot

      Storage information

      Avoid freeze / thaw cycle

      Notes

      Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

      This product is a recombinant monoclonal antibody, which offers several advantages including:

      • - High batch-to-batch consistency and reproducibility
      • - Improved sensitivity and specificity
      • - Long-term security of supply
      • - Animal-free batch production

      For more information, read more on recombinant antibodies.

      Supplementary info

      Activity summary

      Tau also known as microtubule-associated protein Tau (MAPT) plays an important role in stabilizing microtubules in neuronal cells. Tau is primarily found in the central nervous system but also exists in peripheral neurons. Human Tau protein comes in six isoforms due to alternative splicing with molecular weights ranging from 48 kDa to 67 kDa. This protein predominantly locates in the axons of neurons where it maintains the stability of microtubule tracks necessary for axonal transport.

      Biological function summary

      Tau is involved in the assembly and stabilization of microtubules essential for maintaining neuronal structure. It interacts with microtubule-binding domains (MBD) to bind and bundle microtubules facilitating intracellular transport. Tau forms a part of the neuronal cytoskeleton complex working closely with other cytoskeletal proteins to preserve the proper axonal transport and function. Abnormally phosphorylated Tau often termed phospho-Tau disrupts this complex affecting microtubule stability.

      Pathways

      Tau has critical involvement in several signaling cascades such as the microtubule-binding and transport pathways. Glycogen synthase kinase 3 beta (GSK3β) and cyclin-dependent kinase 5 (CDK5) frequently phosphorylate Tau controlling its interaction with microtubules. Phosphorylated Tau accumulates leading to the formation of neurofibrillary tangles often observed in neurodegenerative conditions. Additionally Tau interacts with GAPDH impacting cellular energy regulation through potential pathway cross-talk involving oxidative stress responses.

      Associated diseases and disorders

      Tau is closely associated with Alzheimer's disease and frontotemporal dementia. In Alzheimer's disease hyperphosphorylated Tau aggregates into paired helical filaments forming neurofibrillary tangles while similar aggregates are observed in frontotemporal dementia. In these conditions Tau links to amyloid precursor protein (APP) where misregulated phosphorylation-driven interactions contribute to neurodegeneration. Identifying phospho-Tau and its altered interactions with related proteins aids in understanding and potentially treating these disorders.

      Product promise

      We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

      In the unlikely event of one of our products not working as expected, you are covered by our product promise.

      Full details and terms and conditions can be found here:
      Terms & Conditions.

      7 product images

      • Western blot - Anti-Tau antibody [EP2456Y] (ab76128), expandable thumbnail

        Western blot - Anti-Tau antibody [EP2456Y] (ab76128)

        All lanes: Western blot - Anti-Tau antibody [EP2456Y] (ab76128) at 1/10000 dilution

        Lane 1: Mouse brain lysate at 15 µg

        Lane 2: Rat brain lysate at 15 µg

        Secondary

        All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

        Predicted band size: 78 kDa

      • Western blot - Anti-Tau antibody [EP2456Y] (ab76128), expandable thumbnail

        Western blot - Anti-Tau antibody [EP2456Y] (ab76128)

        All lanes: Western blot - Anti-Tau antibody [EP2456Y] (ab76128) at 1/20000 dilution

        All lanes: Human brain lysate

        Secondary

        All lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

        Predicted band size: 78 kDa

      • Immunocytochemistry/ Immunofluorescence - Anti-Tau antibody [EP2456Y] (ab76128), expandable thumbnail

        Immunocytochemistry/ Immunofluorescence - Anti-Tau antibody [EP2456Y] (ab76128)

        Immunocytochemistry analysis of Neuro-2a (Mouse neuroblastoma neuroblast) cells labeling Tau with purified ab76128 at 1/50 dilution (10 μg/mL). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% TritonX-100. Cells were counterstained with Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 μg/mL). Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) was used as the secondary antibody at 1/1000 (2 μg/mL) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

      • Immunocytochemistry/ Immunofluorescence - Anti-Tau antibody [EP2456Y] (ab76128), expandable thumbnail

        Immunocytochemistry/ Immunofluorescence - Anti-Tau antibody [EP2456Y] (ab76128)

        Immunofluorescence staining of Tau using ab76128 (Unpurified format) in ioGlutamatergic Neurons (Human iPSC-Derived Glutamatergic Neurons, ioGlutamatergic Neurons - Human iPSC-Derived Glutamatergic Neurons ab259259), which were differentiated for 11 days post induction.

        The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% PBS-Tween for 5 mins and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab76128 at 0.5 μg/ml and Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291, Mouse monoclonal [DM1A] to alpha Tubulin, at 1/1000 dilution. Cells were then incubated with Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (shown in green) and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120, Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed at 1/1000 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).

        Images were acquired with the Perkin Elmer Operetta HCA and a maximum intensity projection of confocal sections is shown.

      • Flow Cytometry (Intracellular) - Anti-Tau antibody [EP2456Y] (ab76128), expandable thumbnail

        Flow Cytometry (Intracellular) - Anti-Tau antibody [EP2456Y] (ab76128)

        Intracellular Flow Cytometry analysis of SH-SY5Y (Human neuroblastoma epithelial cell) cells labelling Tau with purified ab76128 at 1/500 dilution (1 μg/mL) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabelled control - Cell without incubation with primary antibody and secondary antibody (Blue).

      • Western blot - Anti-Tau antibody [EP2456Y] (ab76128), expandable thumbnail

        Western blot - Anti-Tau antibody [EP2456Y] (ab76128)

        Blocking and diluting buffer and concentration: 5% NFDM/TBST.

        The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 22366723, PMID: 24113653).

        The expression of Tau (O-GlcNAc S400) is upregulated in response to Thiamet G treatment (PMID: 25336656, PMID: 22366723, PMID: 24113653).

        The identity of the higher MW band at approximately 110 kDa is unknown.

        In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

        In Western blot, Anti-Tau antibody - Total protein control (ab76128) staining at 1/1000 dilution.



        In Western blot, Anti-Tau antibody - Total protein control Anti-6X His tag® antibody [EPR20547] - ChIP Grade (Anti-6X His tag® antibody [EPR20547] - ChIP Grade ab213204) staining at 1/5000 dilution

        All lanes: Western blot - Anti-Tau (O-GlcNAc S400) antibody [EPR24891-48] (Anti-Tau (O-GlcNAc S400) antibody [EPR24891-48] ab320076) at 1/1000 dilution

        Lane 1: 293T (human embryonic kidney epithelial cell) cells transfected with a human wild-type Tau 2N4R expression vector containing a myc-His-tag®, whole cell lysate at 50 µg

        Lane 2: 293T cells transfected with a human wild-type Tau 2N4R expression vector containing a myc-His-tag® were treated with 10 uM Thiamet G for 48 hours, whole cell lysate at 50 µg

        Lane 3: 293T cells transfected with a human Tau 2N4R (S400A mutation) expression vector containing a myc-His-tag®, whole cell lysate at 50 µg

        Lane 4: 293T cells transfected with a human Tau 2N4R (S400A mutation) expression vector containing a myc-His-tag® were treated with 10 uM Thiamet G for 48 hours, whole cell lysate at 50 µg

        Secondary

        All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

        Observed band size: 25-75 kDa, 36 kDa

        Exposure time: 37s

      • Western blot - Anti-Tau antibody [EP2456Y] (ab76128), expandable thumbnail

        Western blot - Anti-Tau antibody [EP2456Y] (ab76128)

        Blocking/dilution buffer: 5% NFDM/TBST

        All lanes: Western blot - Anti-Tau antibody [EP2456Y] (ab76128) at 1/1000 dilution

        Lane 1: 293T cells transfected with an empty vector containing a flag tag whole cell lysate at 20 µg

        Lane 2: 293T cells transfected with a human Tau expression vector containing a flag whole cell lysate at 20 µg

        Lane 3: 293T cells transfected with a human MAP2 expression vector containing a flag whole cell lysate at 20 µg

        Lane 4: 293T cells transfected with a human MAP4 expression vector containing a flag whole cell lysate at 20 µg

        Secondary

        All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

        Predicted band size: 78 kDa

        Observed band size: 55-100 kDa

        Exposure time: 1s

      Downloads

      Product protocols

      For this product, it's our understanding that no specific protocols are required. You can:

      Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

      For licensing inquiries, please contact partnerships@abcam.com