Rabbit Recombinant Monoclonal TAU antibody. Suitable for WB, ICC/IF, Flow Cyt (Intra) and reacts with Mouse, Rat, Human samples. Cited in 22 publications.
IgG
Rabbit
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Liquid
Monoclonal
IP | WB | ICC/IF | Flow Cyt (Intra) | |
---|---|---|---|---|
Human | Not recommended | Tested | Tested | Tested |
Mouse | Not recommended | Tested | Tested | Tested |
Rat | Not recommended | Tested | Tested | Tested |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info 1/10000 | Notes For unpurified use at 1/2000 - 1/10000. |
Species Rat | Dilution info 1/10000 | Notes For unpurified use at 1/2000 - 1/10000. |
Species Human | Dilution info 1/10000 | Notes For unpurified use at 1/2000 - 1/10000. |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info 1/50 | Notes For unpurified use at 1/100 - 1/500. |
Species Rat | Dilution info 1/50 | Notes For unpurified use at 1/100 - 1/500. |
Species Human | Dilution info 1/50 | Notes For unpurified use at 1/100 - 1/500. |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info 1/500 | Notes For unpurified use at 1/160 - 1/1000. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
Species Rat | Dilution info 1/500 | Notes For unpurified use at 1/160 - 1/1000. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
Species Human | Dilution info 1/500 | Notes For unpurified use at 1/160 - 1/1000. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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Promotes microtubule assembly and stability, and might be involved in the establishment and maintenance of neuronal polarity (PubMed:21985311). The C-terminus binds axonal microtubules while the N-terminus binds neural plasma membrane components, suggesting that tau functions as a linker protein between both (PubMed:21985311, PubMed:32961270). Axonal polarity is predetermined by TAU/MAPT localization (in the neuronal cell) in the domain of the cell body defined by the centrosome. The short isoforms allow plasticity of the cytoskeleton whereas the longer isoforms may preferentially play a role in its stabilization.
Microtubule-associated protein tau, Neurofibrillary tangle protein, Paired helical filament-tau, PHF-tau, MAPT, MTBT1, TAU, MAPTL
Rabbit Recombinant Monoclonal TAU antibody. Suitable for WB, ICC/IF, Flow Cyt (Intra) and reacts with Mouse, Rat, Human samples. Cited in 22 publications.
Microtubule-associated protein tau, Neurofibrillary tangle protein, Paired helical filament-tau, PHF-tau, MAPT, MTBT1, TAU, MAPTL
IgG
Rabbit
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Liquid
Monoclonal
EP2456Y
Affinity purification Protein A
This antibody detects Tau, whether phosphorylated or unphosphorylated on serine 635.
The specificity of this antibody refers to P10636-8.
This antibody clone has been shown to react with Tau 4R and 3R isoforms (doi.org/10.1101/2023.04.13.536711).
Our testing suggests that this antibody clone does not cross-reacts with MAP2 or MAP4
1.55 x 10-11 M
Blue Ice
1-2 weeks
+4°C
-20°C
Upon delivery aliquot
Avoid freeze / thaw cycle
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
For more information, read more on recombinant antibodies.
Tau also known as microtubule-associated protein Tau (MAPT) plays an important role in stabilizing microtubules in neuronal cells. Tau is primarily found in the central nervous system but also exists in peripheral neurons. Human Tau protein comes in six isoforms due to alternative splicing with molecular weights ranging from 48 kDa to 67 kDa. This protein predominantly locates in the axons of neurons where it maintains the stability of microtubule tracks necessary for axonal transport.
Tau is involved in the assembly and stabilization of microtubules essential for maintaining neuronal structure. It interacts with microtubule-binding domains (MBD) to bind and bundle microtubules facilitating intracellular transport. Tau forms a part of the neuronal cytoskeleton complex working closely with other cytoskeletal proteins to preserve the proper axonal transport and function. Abnormally phosphorylated Tau often termed phospho-Tau disrupts this complex affecting microtubule stability.
Tau has critical involvement in several signaling cascades such as the microtubule-binding and transport pathways. Glycogen synthase kinase 3 beta (GSK3β) and cyclin-dependent kinase 5 (CDK5) frequently phosphorylate Tau controlling its interaction with microtubules. Phosphorylated Tau accumulates leading to the formation of neurofibrillary tangles often observed in neurodegenerative conditions. Additionally Tau interacts with GAPDH impacting cellular energy regulation through potential pathway cross-talk involving oxidative stress responses.
Tau is closely associated with Alzheimer's disease and frontotemporal dementia. In Alzheimer's disease hyperphosphorylated Tau aggregates into paired helical filaments forming neurofibrillary tangles while similar aggregates are observed in frontotemporal dementia. In these conditions Tau links to amyloid precursor protein (APP) where misregulated phosphorylation-driven interactions contribute to neurodegeneration. Identifying phospho-Tau and its altered interactions with related proteins aids in understanding and potentially treating these disorders.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
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In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
All lanes: Western blot - Anti-Tau antibody [EP2456Y] (ab76128) at 1/10000 dilution
Lane 1: Mouse brain lysate at 15 µg
Lane 2: Rat brain lysate at 15 µg
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 78 kDa
All lanes: Western blot - Anti-Tau antibody [EP2456Y] (ab76128) at 1/20000 dilution
All lanes: Human brain lysate
All lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 78 kDa
Immunocytochemistry analysis of Neuro-2a (Mouse neuroblastoma neuroblast) cells labeling Tau with purified ab76128 at 1/50 dilution (10 μg/mL). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% TritonX-100. Cells were counterstained with Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 μg/mL). Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) was used as the secondary antibody at 1/1000 (2 μg/mL) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
Immunofluorescence staining of Tau using ab76128 (Unpurified format) in ioGlutamatergic Neurons (Human iPSC-Derived Glutamatergic Neurons, ioGlutamatergic Neurons - Human iPSC-Derived Glutamatergic Neurons ab259259), which were differentiated for 11 days post induction.
The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% PBS-Tween for 5 mins and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab76128 at 0.5 μg/ml and Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291, Mouse monoclonal [DM1A] to alpha Tubulin, at 1/1000 dilution. Cells were then incubated with Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (shown in green) and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120, Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed at 1/1000 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).
Images were acquired with the Perkin Elmer Operetta HCA and a maximum intensity projection of confocal sections is shown.
Intracellular Flow Cytometry analysis of SH-SY5Y (Human neuroblastoma epithelial cell) cells labelling Tau with purified ab76128 at 1/500 dilution (1 μg/mL) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabelled control - Cell without incubation with primary antibody and secondary antibody (Blue).
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 22366723, PMID: 24113653).
The expression of Tau (O-GlcNAc S400) is upregulated in response to Thiamet G treatment (PMID: 25336656, PMID: 22366723, PMID: 24113653).
The identity of the higher MW band at approximately 110 kDa is unknown.
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
In Western blot, Anti-Tau antibody - Total protein control (ab76128) staining at 1/1000 dilution.
In Western blot, Anti-Tau antibody - Total protein control Anti-6X His tag® antibody [EPR20547] - ChIP Grade (Anti-6X His tag® antibody [EPR20547] - ChIP Grade ab213204) staining at 1/5000 dilution
All lanes: Western blot - Anti-Tau (O-GlcNAc S400) antibody [EPR24891-48] (Anti-Tau (O-GlcNAc S400) antibody [EPR24891-48] ab320076) at 1/1000 dilution
Lane 1: 293T (human embryonic kidney epithelial cell) cells transfected with a human wild-type Tau 2N4R expression vector containing a myc-His-tag®, whole cell lysate at 50 µg
Lane 2: 293T cells transfected with a human wild-type Tau 2N4R expression vector containing a myc-His-tag® were treated with 10 uM Thiamet G for 48 hours, whole cell lysate at 50 µg
Lane 3: 293T cells transfected with a human Tau 2N4R (S400A mutation) expression vector containing a myc-His-tag®, whole cell lysate at 50 µg
Lane 4: 293T cells transfected with a human Tau 2N4R (S400A mutation) expression vector containing a myc-His-tag® were treated with 10 uM Thiamet G for 48 hours, whole cell lysate at 50 µg
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Observed band size: 25-75 kDa, 36 kDa
Exposure time: 37s
Blocking/dilution buffer: 5% NFDM/TBST
All lanes: Western blot - Anti-Tau antibody [EP2456Y] (ab76128) at 1/1000 dilution
Lane 1: 293T cells transfected with an empty vector containing a flag tag whole cell lysate at 20 µg
Lane 2: 293T cells transfected with a human Tau expression vector containing a flag whole cell lysate at 20 µg
Lane 3: 293T cells transfected with a human MAP2 expression vector containing a flag whole cell lysate at 20 µg
Lane 4: 293T cells transfected with a human MAP4 expression vector containing a flag whole cell lysate at 20 µg
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 78 kDa
Observed band size: 55-100 kDa
Exposure time: 1s
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