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Rabbit Recombinant Monoclonal TAU antibody. Carrier free. Suitable for WB, ICC/IF, Flow Cyt (Intra) and reacts with Mouse, Rat, Human samples.

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Images

Western blot - Anti-Tau antibody [EP2456Y] - BSA and Azide free (AB174727), expandable thumbnail
  • Western blot - Anti-Tau antibody [EP2456Y] - BSA and Azide free (AB174727), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-Tau antibody [EP2456Y] - BSA and Azide free (AB174727), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-Tau antibody [EP2456Y] - BSA and Azide free (AB174727), expandable thumbnail
  • Western blot - Anti-Tau antibody [EP2456Y] - BSA and Azide free (AB174727), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

Constituents: PBS

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPWBICC/IFFlow Cyt (Intra)
Human
Not recommended
Expected
Tested
Tested
Mouse
Not recommended
Tested
Tested
Tested
Rat
Not recommended
Tested
Tested
Tested

Not recommended
Not recommended

Species

Mouse, Rat, Human

Dilution info

-

Notes

-

Tested
Tested

Species

Mouse, Rat

Dilution info

-

Notes

-

Expected
Expected

Species

Human

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Mouse, Rat, Human

Dilution info

-

Notes

-

Tested
Tested

Species

Mouse

Dilution info

-

Notes

ab199376 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Species

Rat

Dilution info

-

Notes

ab199376 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Species

Human

Dilution info

-

Notes

ab199376 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Associated Products

Select an associated product type

4 products for Alternative Version

    8 products for Alternative Product

      Target data

      Function

      Promotes microtubule assembly and stability, and might be involved in the establishment and maintenance of neuronal polarity (PubMed:21985311). The C-terminus binds axonal microtubules while the N-terminus binds neural plasma membrane components, suggesting that tau functions as a linker protein between both (PubMed:21985311, PubMed:32961270). Axonal polarity is predetermined by TAU/MAPT localization (in the neuronal cell) in the domain of the cell body defined by the centrosome. The short isoforms allow plasticity of the cytoskeleton whereas the longer isoforms may preferentially play a role in its stabilization.

      Alternative names

      Microtubule-associated protein tau, Neurofibrillary tangle protein, Paired helical filament-tau, PHF-tau, MAPT, MTBT1, TAU, MAPTL

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      Rabbit Recombinant Monoclonal TAU antibody. Carrier free. Suitable for WB, ICC/IF, Flow Cyt (Intra) and reacts with Mouse, Rat, Human samples.

      Alternative names

      Microtubule-associated protein tau, Neurofibrillary tangle protein, Paired helical filament-tau, PHF-tau, MAPT, MTBT1, TAU, MAPTL

      Key facts

      Isotype

      IgG

      Form

      Liquid

      Clonality

      Monoclonal

      Immunogen
      • The exact immunogen used to generate this antibody is proprietary information.
      Carrier free

      Yes

      Clone number

      EP2456Y

      Purification technique

      Affinity purification Protein A

      Specificity

      This antibody detects Tau, whether phosphorylated or unphosphorylated on serine 635.
      The specificity of this antibody refers to P10636-8.
      This antibody clone has been shown to react with Tau 4R and 3R isoforms (doi.org/10.1101/2023.04.13.536711).
      Our testing suggests that this antibody clone does not cross-reacts with MAP2 or MAP4

      Dissociation constant

      1.55 x 10-11 M

      Concentration
      Loading...

      Storage

      Shipped at conditions

      Blue Ice

      Appropriate long-term storage conditions

      +4°C

      Storage information

      Do Not Freeze

      Notes

      ab174727 is the carrier-free version of Anti-Tau antibody [EP2456Y] ab76128.

      Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

      This product is a recombinant monoclonal antibody, which offers several advantages including:

      • - High batch-to-batch consistency and reproducibility
      • - Improved sensitivity and specificity
      • - Long-term security of supply
      • - Animal-free batch production

      For more information, read more on recombinant antibodies.

      Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

      This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

      Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

      This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

      Supplementary info

      Activity summary

      Tau also known as microtubule-associated protein Tau (MAPT) plays an important role in stabilizing microtubules in neuronal cells. Tau is primarily found in the central nervous system but also exists in peripheral neurons. Human Tau protein comes in six isoforms due to alternative splicing with molecular weights ranging from 48 kDa to 67 kDa. This protein predominantly locates in the axons of neurons where it maintains the stability of microtubule tracks necessary for axonal transport.

      Biological function summary

      Tau is involved in the assembly and stabilization of microtubules essential for maintaining neuronal structure. It interacts with microtubule-binding domains (MBD) to bind and bundle microtubules facilitating intracellular transport. Tau forms a part of the neuronal cytoskeleton complex working closely with other cytoskeletal proteins to preserve the proper axonal transport and function. Abnormally phosphorylated Tau often termed phospho-Tau disrupts this complex affecting microtubule stability.

      Pathways

      Tau has critical involvement in several signaling cascades such as the microtubule-binding and transport pathways. Glycogen synthase kinase 3 beta (GSK3β) and cyclin-dependent kinase 5 (CDK5) frequently phosphorylate Tau controlling its interaction with microtubules. Phosphorylated Tau accumulates leading to the formation of neurofibrillary tangles often observed in neurodegenerative conditions. Additionally Tau interacts with GAPDH impacting cellular energy regulation through potential pathway cross-talk involving oxidative stress responses.

      Associated diseases and disorders

      Tau is closely associated with Alzheimer's disease and frontotemporal dementia. In Alzheimer's disease hyperphosphorylated Tau aggregates into paired helical filaments forming neurofibrillary tangles while similar aggregates are observed in frontotemporal dementia. In these conditions Tau links to amyloid precursor protein (APP) where misregulated phosphorylation-driven interactions contribute to neurodegeneration. Identifying phospho-Tau and its altered interactions with related proteins aids in understanding and potentially treating these disorders.

      Product promise

      We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

      In the unlikely event of one of our products not working as expected, you are covered by our product promise.

      Full details and terms and conditions can be found here:
      Terms & Conditions.

      5 product images

      • Western blot - Anti-Tau antibody [EP2456Y] - BSA and Azide free (ab174727), expandable thumbnail

        Western blot - Anti-Tau antibody [EP2456Y] - BSA and Azide free (ab174727)

        All lanes: Western blot - Anti-Tau antibody [EP2456Y] (Anti-Tau antibody [EP2456Y] ab76128) at 1/10000 dilution

        Lane 1: Mouse brain lysate at 15 µg

        Lane 2: Rat brain lysate at 15 µg

        Secondary

        All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

        Predicted band size: 78 kDa

      • Western blot - Anti-Tau antibody [EP2456Y] - BSA and Azide free (ab174727), expandable thumbnail

        Western blot - Anti-Tau antibody [EP2456Y] - BSA and Azide free (ab174727)

        All lanes: Western blot - Anti-Tau antibody [EP2456Y] (Anti-Tau antibody [EP2456Y] ab76128) at 1/20000 dilution

        All lanes: Human brain lysate

        Secondary

        All lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

        Predicted band size: 78 kDa

      • Immunocytochemistry/ Immunofluorescence - Anti-Tau antibody [EP2456Y] - BSA and Azide free (ab174727), expandable thumbnail

        Immunocytochemistry/ Immunofluorescence - Anti-Tau antibody [EP2456Y] - BSA and Azide free (ab174727)

        This data was developed using Anti-Tau antibody [EP2456Y] ab76128, the same antibody clone in a different buffer formulation.

        Immunocytochemistry analysis of Neuro-2a (Mouse neuroblastoma neuroblast) cells labeling Tau with purified Anti-Tau antibody [EP2456Y] ab76128 at 1/50 dilution (10 μg/mL). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% TritonX-100. Cells were counterstained with Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 μg/mL). Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) was used as the secondary antibody at 1/1000 (2 μg/mL) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

      • Flow Cytometry (Intracellular) - Anti-Tau antibody [EP2456Y] - BSA and Azide free (ab174727), expandable thumbnail

        Flow Cytometry (Intracellular) - Anti-Tau antibody [EP2456Y] - BSA and Azide free (ab174727)

        This data was developed using Anti-Tau antibody [EP2456Y] ab76128, the same antibody clone in a different buffer formulation. Intracellular Flow Cytometry analysis of SH-SY5Y (Human neuroblastoma epithelial cell) cells labelling Tau with purified Anti-Tau antibody [EP2456Y] ab76128 at 1/500 dilution (1 μg/mL) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabelled control - Cell without incubation with primary antibody and secondary antibody (Blue).

      • Western blot - Anti-Tau antibody [EP2456Y] - BSA and Azide free (ab174727), expandable thumbnail

        Western blot - Anti-Tau antibody [EP2456Y] - BSA and Azide free (ab174727)

        This data was developed using Anti-Tau antibody [EP2456Y] ab76128, the same antibody clone in a different buffer formulation. Blocking/dilution buffer: 5% NFDM/TBST

        All lanes: Western blot - Anti-Tau antibody [EP2456Y] (Anti-Tau antibody [EP2456Y] ab76128) at 1/1000 dilution

        Lane 1: 293T cells transfected with an empty vector containing a flag tag whole cell lysate at 20 µg

        Lane 2: 293T cells transfected with a human Tau expression vector containing a flag whole cell lysate at 20 µg

        Lane 3: 293T cells transfected with a human MAP2 expression vector containing a flag whole cell lysate at 20 µg

        Lane 4: 293T cells transfected with a human MAP4 expression vector containing a flag whole cell lysate at 20 µg

        Secondary

        All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

        Predicted band size: 78 kDa

        Observed band size: 55-100 kDa

        Exposure time: 1s

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      Product protocols

      For this product, it's our understanding that no specific protocols are required. You can:

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