Name: Anti-Tau antibody [EPR22524-95]
SKU: ab254256
Rating: 5 (3 reviews)

Anti-Tau antibody [EPR22524-95] (ab254256) is a rabbit monoclonal antibody detecting Tau in Western Blot, IP, IHC-P, IHC-Fr. Suitable for Human, Mouse.

- Biophysical QC for unrivalled batch-batch consistency

Images

Immunoprecipitation - Anti-Tau antibody [EPR22524-95] (AB254256), expandable thumbnail

Publications

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	IP	WB	IHC-Fr	IHC-P
Human	Tested	Tested	Expected	Tested
Mouse	Expected	Tested	Tested	Tested

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/30	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Expected
Expected

Species	Dilution info	Notes
Species Mouse	Dilution info Use at an assay dependent concentration.	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/1000	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Human	Dilution info 1/1000	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/100	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Human	Dilution info Use at an assay dependent concentration.	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/4000	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Human	Dilution info 1/4000	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Associated Products

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6 products for Alternative Product

Target data

See full target information Mapt

Function

Promotes microtubule assembly and stability, and might be involved in the establishment and maintenance of neuronal polarity. The C-terminus binds axonal microtubules while the N-terminus binds neural plasma membrane components, suggesting that tau functions as a linker protein between both. Axonal polarity is predetermined by tau localization (in the neuronal cell) in the domain of the cell body defined by the centrosome. The short isoforms allow plasticity of the cytoskeleton whereas the longer isoforms may preferentially play a role in its stabilization.

Alternative names

Mtapt, Tau, Mapt, Microtubule-associated protein tau, Neurofibrillary tangle protein, Paired helical filament-tau, PHF-tau

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Clone number

EPR22524-95

Purification technique

Affinity purification Protein A

Specificity

The specificity of this antibody refers to P10637-1.

Our testing suggests that this antibody clone does not cross-reacts with MAP2 or MAP4.

Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage duration: 1-2 weeks
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: -20°C
Aliquoting information: Upon delivery aliquot
Storage information: Avoid freeze / thaw cycle

Notes

What is this antibody validated in?

Anti-Tau antibody [EPR22524-95] (ab254256) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC-P), Immunohistochemistry (IHC-Fr) in Human, Mouse samples.

What is the molecular weight of Tau?

Anti-Tau [EPR22524-95] (ab254256) specifically detects a band for Tau (UniProt: P10636) at a molecular weight of 76kDa.

Trial sizes available!

Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.

Other related products

We have a range of other formats of antibody clone [EPR22524-95] also available for your convenience:
ab254256, Carrier free - ab255271, ab317841, Carrier free - ab317842

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Tau also known as microtubule-associated protein Tau (MAPT) plays an important role in stabilizing microtubules in neuronal cells. Tau is primarily found in the central nervous system but also exists in peripheral neurons. Human Tau protein comes in six isoforms due to alternative splicing with molecular weights ranging from 48 kDa to 67 kDa. This protein predominantly locates in the axons of neurons where it maintains the stability of microtubule tracks necessary for axonal transport.

Biological function summary

Tau is involved in the assembly and stabilization of microtubules essential for maintaining neuronal structure. It interacts with microtubule-binding domains (MBD) to bind and bundle microtubules facilitating intracellular transport. Tau forms a part of the neuronal cytoskeleton complex working closely with other cytoskeletal proteins to preserve the proper axonal transport and function. Abnormally phosphorylated Tau often termed phospho-Tau disrupts this complex affecting microtubule stability.

Pathways

Tau has critical involvement in several signaling cascades such as the microtubule-binding and transport pathways. Glycogen synthase kinase 3 beta (GSK3Β) and cyclin-dependent kinase 5 (CDK5) frequently phosphorylate Tau controlling its interaction with microtubules. Phosphorylated Tau accumulates leading to the formation of neurofibrillary tangles often observed in neurodegenerative conditions. Additionally Tau interacts with GAPDH impacting cellular energy regulation through potential pathway cross-talk involving oxidative stress responses.

Associated diseases and disorders

Tau is closely associated with Alzheimer's disease and frontotemporal dementia. In Alzheimer's disease hyperphosphorylated Tau aggregates into paired helical filaments forming neurofibrillary tangles while similar aggregates are observed in frontotemporal dementia. In these conditions Tau links to amyloid precursor protein (APP) where misregulated phosphorylation-driven interactions contribute to neurodegeneration. Identifying phospho-Tau and its altered interactions with related proteins aids in understanding and potentially treating these disorders.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

13 product images

Immunoprecipitation - Anti-Tau antibody [EPR22524-95] (ab254256)
Tau was immunoprecipitated from 0.35 mg human brain lysate with ab254256 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab254256 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366), was used at 1/5000 dilution.
Lane 1: Human brain lysate 10 μg (Input).
Lane 2: ab254256 IP in human brain lysate.
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab254256 in human brain lysate.
Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure time: 10 seconds.
All lanes: Immunoprecipitation - Anti-Tau antibody [EPR22524-95] (ab254256)
Predicted band size: 78 kDa
Observed band size: 50 kDa
Western blot - Anti-Tau antibody [EPR22524-95] (ab254256)
Negative control: human fetal heart, human fetal kidney, human spleen. (PMID: 24386422).
The molecular weight observed is consistent with what has been described in the literature (PMID: 24386422).
Blocking/Dilution buffer: 5% NFDM/TBST.
All lanes: Western blot - Anti-Tau antibody [EPR22524-95] (ab254256) at 1/1000 dilution
Lane 1: Human brain lysate at 10 µg
Lane 2: Human fetal heart lysate at 10 µg
Lane 3: Human fetal kidney lysate at 10 µg
Lane 4: Human spleen lysate at 10 µg
Secondary
All lanes: Western blot - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/1000 dilution
Predicted band size: 78 kDa
Observed band size: 50-70 kDa
Exposure time: 8s
Western blot - Anti-Tau antibody [EPR22524-95] (ab254256)
Negative control: mouse heart, mouse kidney, mouse spleen. (PMID: 24386422).
The molecular weight observed is consistent with what has been described in the literature (PMID: 24386422).
Blocking/Dilution buffer: 5% NFDM/TBST.
All lanes: Western blot - Anti-Tau antibody [EPR22524-95] (ab254256) at 1/1000 dilution
Lane 1: Mouse brain lysate at 10 µg
Lane 2: Mouse heart lysate at 10 µg
Lane 3: Mouse kidney lysate at 10 µg
Lane 4: Mouse spleen lysate at 10 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Predicted band size: 78 kDa
Observed band size: 50-70 kDa
Exposure time: 15s
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau antibody [EPR22524-95] (ab254256)
Immunohistochemical analysis of paraffin-embedded mouse hippocampus tissue labeling Tau with ab254256 at 1/4000 dilution, followed by ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Positive staining on mouse hippocampus (PMID: 22961084) is observed. Counterstained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
The section was incubated with ab254256 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument.
Immunohistochemistry (Frozen sections) - Anti-Tau antibody [EPR22524-95] (ab254256)
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse cerebral cortex tissue labeling Tau with ab254256 at 1/100 dilution (green), followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 AlexaFluor^®488 Goat anti-Rabbit secondary at a 1/1,000 dilution. Positive staining on mouse cerebral cortex (PMID: 22961084) is observed. Counterstained with DAPI (blue).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 AlexaFluor^®488 Goat anti-Rabbit used at a 1/1,000 dilution.
Heat mediated antigen retrieval using sodium citrate buffer (10 mM citrate pH 6.0 and 0.05% Tween-20).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau antibody [EPR22524-95] (ab254256)
Immunohistochemical analysis of paraffin-embedded mouse cerebral cortex tissue labeling Tau with ab254256 at 1/4000 dilution, followed by ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Positive staining on mouse cerebral cortex (PMID: 22961084) is observed. Counterstained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
The section was incubated with ab254256 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau antibody [EPR22524-95] (ab254256)
Immunohistochemical analysis of paraffin-embedded human cerebral cortex tissue labeling Tau with ab254256 at 1/4000 dilution, followed by ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Positive staining on human cerebral cortex (PMID: 22961084) is observed. Counterstained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
The section was incubated with ab254256 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument.
Western blot - Anti-Tau antibody [EPR22524-95] (ab254256)
Blocking/dilution buffer: 5% NFDM/TBST
All lanes: Western blot - Anti-Tau antibody [EPR22524-95] (ab254256) at 1/1000 dilution
Lane 1: 293T cells transfected with an empty vector containing a flag tag whole cell lysate at 20 µg
Lane 2: 293T cells transfected with a human Tau expression vector containing a flag whole cell lysate at 20 µg
Lane 3: 293T cells transfected with a human MAP2 expression vector containing a flag whole cell lysate at 20 µg
Lane 4: 293T cells transfected with a human MAP4 expression vector containing a flag whole cell lysate at 20 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 78 kDa
Observed band size: 55-100 kDa
Exposure time: 1s
Western blot - Anti-Tau antibody [EPR22524-95] (ab254256)
Blocking and diluting buffer and concentration: 5% NFDM/TBST.

In Western blot, anti-Tau antibody (ab254256) loading control staining at 1/1000 dilution.

Exposure time: 3.25 seconds.
All lanes: Western blot - Anti-Tau (phospho S396) antibody [E178] - Mouse IgG2a (Chimeric) - BSA and Azide free (Anti-Tau (phospho S396) antibody [E178] - Mouse IgG2a (Chimeric) - BSA and Azide free ab302491) at 1/1000 dilution
Lane 1: Mouse brain tissue lysate (untreated membrane) at 10 µg
Lane 2: Mouse brain tissue lysate (phosphatase treated membrane) at 10 µg
Secondary
All lanes: Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/5000 dilution
Observed band size: 50-70 kDa
Western blot - Anti-Tau antibody [EPR22524-95] (ab254256)
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
In Western blot, Anti-Tau antibody - Total protein control (ab254256) staining at 1/1000 dilution.
In Western blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (Anti-6X His tag® antibody [EPR20547] - ChIP Grade ab213204) staining at 1/5000 dilution.
All lanes: Western blot - Anti-Tau (nitrosyl Y29) antibody [EPR27841-79] (Anti-Tau (nitrosyl Y29) antibody [EPR27841-79] ab315891) at 1/1000 dilution
Lane 1: Untreated 293T cells transfected with an empty vector containing a His-tag, whole cell lysate at 20 µg with 5% NFDM/TBST
Lane 2: 293T cells transfected with an empty vector containing a His-tag were treated with 3mM peroxynitrite for 1 hour, whole cell lysate at 20 µg with 5% NFDM/TBST
Lane 3: Untreated 293T cells transfected with a human Tau 2N4R expression vector containing a His-tag, whole cell lysate at 20 µg with 5% NFDM/TBST
Lane 4: 293T cells transfected with a human Tau 2N4R expression vector containing a His-tag were treated with 3mM peroxynitrite for 1 hour, whole cell lysate at 20 µg with 5% NFDM/TBST
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 20-75 kDa, 36 kDa
Exposure time: 180s
Western blot - Anti-Tau antibody [EPR22524-95] (ab254256)
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
In Western blot, Anti-Histone H3 antibody [EPR16987] - Nuclear Marker and ChIP Grade (Anti-Histone H3 antibody [EPR16987] - Nuclear Marker and ChIP Grade ab176842) staining at 1/100000 dilution.
In Western blot, Anti-6X His tag antibody [EPR20547]-CHIP Grade (Anti-6X His tag® antibody [EPR20547] - ChIP Grade ab213204) staining at 1/5000 dilution, Anti-Tau antibody [EPR22524-95] (ab254256) staining at 1/1000 dilution.
All lanes: Western blot - Anti-1N Tau antibody [EPR25206-82] (Anti-1N Tau antibody [EPR25206-82] ab314889) at 1/1000 dilution
Lane 1: 293T cells transfected with an empty vector containing a His-tag, whole cell lysate at 20 µg
Lane 2: 293T cells transfected with a human Tau 0N3R expression vector containing a His-tag, whole cell lysate at 20 µg
Lane 3: 293T cells transfected with a human Tau 0N4R expression vector containing a His-tag, whole cell lysate at 20 µg
Lane 4: 293T cells transfected with a human Tau 1N3R expression vector containing a His-tag, whole cell lysate at 20 µg
Lane 5: 293T cells transfected with a human Tau 1N4R expression vector containing a His-tag, whole cell lysate at 20 µg
Lane 6: 293T cells transfected with a human Tau 2N3R expression vector containing a His-tag, whole cell lysate at 20 µg
Lane 7: 293T cells transfected with a human Tau 2N4R expression vector containing a His-tag, whole cell lysate at 20 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 37-75 kDa, 15 kDa
Exposure time: 6s
Western blot - Anti-Tau antibody [EPR22524-95] (ab254256)
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
In Western blot, Anti-Tau antibody [EPR22524-95] (ab254256) staining at 1/1000 dilution.
All lanes: Western blot - Anti-1N Tau antibody [EPR25206-82] (Anti-1N Tau antibody [EPR25206-82] ab314889) at 1/1000 dilution
Lane 1: His-tagged Tau 0N3R human recombinant protein at 50 ng
Lane 2: His-tagged Tau 0N4R human recombinant protein at 50 ng
Lane 3: His-tagged Tau 1N3R human recombinant protein at 10 ng
Lane 4: His-tagged Tau 1N4R human recombinant protein at 10 ng
Lane 5: His-tagged Tau 2N3R human recombinant protein at 50 ng
Lane 6: His-tagged Tau 2N4R human recombinant protein at 50 ng
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 75 kDa
Exposure time: 180s
Western blot - Anti-Tau antibody [EPR22524-95] (ab254256)
Tau Western blot staining using rabbit Anti-Tau antibody
Blocking and diluting buffer and concentration: 5% NFDM/TBST.

Tau assembles into oligomers as described in PMID: 28382304, 32692785 and 30120733.

In Western blot, anti-Tau antibody (ab254256) loading control staining at 1/1000 dilution.

Exposure time: 26 seconds.
All lanes: Western blot - Anti-Tau (phospho S396) antibody [E178] - Mouse IgG2a (Chimeric) - BSA and Azide free (Anti-Tau (phospho S396) antibody [E178] - Mouse IgG2a (Chimeric) - BSA and Azide free ab302491) at 1/1000 dilution
Lane 1: Rat brain tissue lysate (untreated membrane) at 10 µg
Lane 2: Rat brain tissue lysate (phosphatase treated membrane) at 10 µg
Secondary
All lanes: Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/5000 dilution
Observed band size: 50-60 kDa, 200 kDa