Rabbit Recombinant Monoclonal TAU antibody. Carrier free. Suitable for IP, WB, IHC-Fr, IHC-P and reacts with Human, Mouse samples.
Images
![Immunoprecipitation - Anti-Tau antibody [EPR22524-95] - BSA and Azide free (AB255271), expandable thumbnail](https://content.abcam.com/products/images/tau-antibody-epr22524-95-bsa-and-azide-free-ab255271--immunoprecipitation-img3681.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-Tau antibody [EPR22524-95] - BSA and Azide free (AB255271)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau antibody [EPR22524-95] - BSA and Azide free (AB255271), expandable thumbnail](https://content.abcam.com/products/images/tau-antibody-epr22524-95-bsa-and-azide-free-ab255271--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img113074.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau antibody [EPR22524-95] - BSA and Azide free (AB255271)")
![Immunohistochemistry (Frozen sections) - Anti-Tau antibody [EPR22524-95] - BSA and Azide free (AB255271), expandable thumbnail](https://content.abcam.com/products/images/tau-antibody-epr22524-95-bsa-and-azide-free-ab255271--immunohistochemistry-frozen-sections-img134536.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Frozen sections) - Anti-Tau antibody [EPR22524-95] - BSA and Azide free (AB255271)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau antibody [EPR22524-95] - BSA and Azide free (AB255271), expandable thumbnail](https://content.abcam.com/products/images/tau-antibody-epr22524-95-bsa-and-azide-free-ab255271--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img58096.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau antibody [EPR22524-95] - BSA and Azide free (AB255271)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau antibody [EPR22524-95] - BSA and Azide free (AB255271), expandable thumbnail](https://content.abcam.com/products/images/tau-antibody-epr22524-95-bsa-and-azide-free-ab255271--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img103906.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau antibody [EPR22524-95] - BSA and Azide free (AB255271)")
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
Liquid
- Clonality
Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | WB | IHC-Fr | IHC-P | |
|---|---|---|---|---|
| Human | Tested | Expected | Expected | Expected |
| Mouse | Expected | Expected | Tested | Tested |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
WB
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human | Dilution info Use at an assay dependent concentration. | Notes - |
IHC-Fr
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
Select an associated product type
6 products for Alternative Product
Target data
Function
Promotes microtubule assembly and stability, and might be involved in the establishment and maintenance of neuronal polarity. The C-terminus binds axonal microtubules while the N-terminus binds neural plasma membrane components, suggesting that tau functions as a linker protein between both. Axonal polarity is predetermined by tau localization (in the neuronal cell) in the domain of the cell body defined by the centrosome. The short isoforms allow plasticity of the cytoskeleton whereas the longer isoforms may preferentially play a role in its stabilization.
Alternative names
Microtubule-associated protein tau, Neurofibrillary tangle protein, Paired helical filament-tau, PHF-tau, Tau, Mtapt, Mapt
Recommended products
Rabbit Recombinant Monoclonal TAU antibody. Carrier free. Suitable for IP, WB, IHC-Fr, IHC-P and reacts with Human, Mouse samples.
Alternative names
Microtubule-associated protein tau, Neurofibrillary tangle protein, Paired helical filament-tau, PHF-tau, Tau, Mtapt, Mapt
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
Yes
- Clone number
EPR22524-95
- Purification technique
Affinity purification Protein A
- Specificity
The specificity of this antibody refers to P10637-1.
Our testing suggests that this antibody clone does not cross-reacts with MAP2 or MAP4.
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
+4°C
- Storage information
Do Not Freeze
Notes
ab255271 is the carrier-free version of Anti-Tau antibody [EPR22524-95] ab254256.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Tau also known as microtubule-associated protein Tau (MAPT) plays an important role in stabilizing microtubules in neuronal cells. Tau is primarily found in the central nervous system but also exists in peripheral neurons. Human Tau protein comes in six isoforms due to alternative splicing with molecular weights ranging from 48 kDa to 67 kDa. This protein predominantly locates in the axons of neurons where it maintains the stability of microtubule tracks necessary for axonal transport.
- Biological function summary
Tau is involved in the assembly and stabilization of microtubules essential for maintaining neuronal structure. It interacts with microtubule-binding domains (MBD) to bind and bundle microtubules facilitating intracellular transport. Tau forms a part of the neuronal cytoskeleton complex working closely with other cytoskeletal proteins to preserve the proper axonal transport and function. Abnormally phosphorylated Tau often termed phospho-Tau disrupts this complex affecting microtubule stability.
- Pathways
Tau has critical involvement in several signaling cascades such as the microtubule-binding and transport pathways. Glycogen synthase kinase 3 beta (GSK3β) and cyclin-dependent kinase 5 (CDK5) frequently phosphorylate Tau controlling its interaction with microtubules. Phosphorylated Tau accumulates leading to the formation of neurofibrillary tangles often observed in neurodegenerative conditions. Additionally Tau interacts with GAPDH impacting cellular energy regulation through potential pathway cross-talk involving oxidative stress responses.
- Associated diseases and disorders
Tau is closely associated with Alzheimer's disease and frontotemporal dementia. In Alzheimer's disease hyperphosphorylated Tau aggregates into paired helical filaments forming neurofibrillary tangles while similar aggregates are observed in frontotemporal dementia. In these conditions Tau links to amyloid precursor protein (APP) where misregulated phosphorylation-driven interactions contribute to neurodegeneration. Identifying phospho-Tau and its altered interactions with related proteins aids in understanding and potentially treating these disorders.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
6 product images
![Immunoprecipitation - Anti-Tau antibody [EPR22524-95] - BSA and Azide free (ab255271), expandable thumbnail](https://content.abcam.com/products/images/tau-antibody-epr22524-95-bsa-and-azide-free-ab255271--immunoprecipitation-img3681.jpg "Immunoprecipitation - Anti-Tau antibody [EPR22524-95] - BSA and Azide free (ab255271)")
Immunoprecipitation - Anti-Tau antibody [EPR22524-95] - BSA and Azide free (ab255271)
Tau was immunoprecipitated from 0.35 mg human brain lysate with Anti-Tau antibody [EPR22524-95] ab254256 at 1/30 dilution. Western blot was performed from the immunoprecipitate using Anti-Tau antibody [EPR22524-95] ab254256 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366), was used at 1/5000 dilution.
Lane 1: Human brain lysate 10 μg (Input).
Lane 2: Anti-Tau antibody [EPR22524-95] ab254256 IP in human brain lysate.
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-Tau antibody [EPR22524-95] ab254256 in human brain lysate.Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure time: 10 seconds.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Tau antibody [EPR22524-95] ab254256).
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
All lanes: Immunoprecipitation - Anti-Tau antibody [EPR22524-95] (Anti-Tau antibody [EPR22524-95] ab254256)
Predicted band size: 78 kDa
Observed band size: 50 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau antibody [EPR22524-95] - BSA and Azide free (ab255271), expandable thumbnail](https://content.abcam.com/products/images/tau-antibody-epr22524-95-bsa-and-azide-free-ab255271--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img113074.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau antibody [EPR22524-95] - BSA and Azide free (ab255271)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau antibody [EPR22524-95] - BSA and Azide free (ab255271)
Immunohistochemical analysis of paraffin-embedded mouse hippocampus tissue labeling Tau with Anti-Tau antibody [EPR22524-95] ab254256 at 1/4000 dilution, followed by ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Positive staining on mouse hippocampus (PMID: 22961084) is observed. Counterstained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
The section was incubated with Anti-Tau antibody [EPR22524-95] ab254256 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Tau antibody [EPR22524-95] ab254256).
![Immunohistochemistry (Frozen sections) - Anti-Tau antibody [EPR22524-95] - BSA and Azide free (ab255271), expandable thumbnail](https://content.abcam.com/products/images/tau-antibody-epr22524-95-bsa-and-azide-free-ab255271--immunohistochemistry-frozen-sections-img134536.jpg "Immunohistochemistry (Frozen sections) - Anti-Tau antibody [EPR22524-95] - BSA and Azide free (ab255271)")
Immunohistochemistry (Frozen sections) - Anti-Tau antibody [EPR22524-95] - BSA and Azide free (ab255271)
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse cerebral cortex tissue labeling Tau with Anti-Tau antibody [EPR22524-95] ab254256 at 1/100 dilution (green), followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at a 1/1,000 dilution. Positive staining on mouse cerebral cortex (PMID: 22961084) is observed. Counterstained with DAPI (blue).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 AlexaFluor®488 Goat anti-Rabbit used at a 1/1,000 dilution.
Heat mediated antigen retrieval using sodium citrate buffer (10 mM citrate pH 6.0 and 0.05% Tween-20).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Tau antibody [EPR22524-95] ab254256).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau antibody [EPR22524-95] - BSA and Azide free (ab255271), expandable thumbnail](https://content.abcam.com/products/images/tau-antibody-epr22524-95-bsa-and-azide-free-ab255271--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img58096.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau antibody [EPR22524-95] - BSA and Azide free (ab255271)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau antibody [EPR22524-95] - BSA and Azide free (ab255271)
Immunohistochemical analysis of paraffin-embedded mouse cerebral cortex tissue labeling Tau with Anti-Tau antibody [EPR22524-95] ab254256 at 1/4000 dilution, followed by ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Positive staining on mouse cerebral cortex (PMID: 22961084) is observed. Counterstained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
The section was incubated with Anti-Tau antibody [EPR22524-95] ab254256 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Tau antibody [EPR22524-95] ab254256).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau antibody [EPR22524-95] - BSA and Azide free (ab255271), expandable thumbnail](https://content.abcam.com/products/images/tau-antibody-epr22524-95-bsa-and-azide-free-ab255271--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img103906.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau antibody [EPR22524-95] - BSA and Azide free (ab255271)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau antibody [EPR22524-95] - BSA and Azide free (ab255271)
Immunohistochemical analysis of paraffin-embedded human cerebral cortex tissue labeling Tau with Anti-Tau antibody [EPR22524-95] ab254256 at 1/4000 dilution, followed by ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Positive staining on human cerebral cortex (PMID: 22961084) is observed. Counterstained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
The section was incubated with Anti-Tau antibody [EPR22524-95] ab254256 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Tau antibody [EPR22524-95] ab254256).
![Western blot - Anti-Tau antibody [EPR22524-95] - BSA and Azide free (ab255271), expandable thumbnail](https://content.abcam.com/products/images/tau-antibody-epr22524-95-bsa-and-azide-free-ab255271--western-blot-img424819.jpg "Western blot - Anti-Tau antibody [EPR22524-95] - BSA and Azide free (ab255271)")
Western blot - Anti-Tau antibody [EPR22524-95] - BSA and Azide free (ab255271)
This data was developed using Anti-Tau antibody [EPR22524-95] ab254256, the same antibody clone in a different buffer formulation
Blocking/dilution buffer: 5% NFDM/TBSTAll lanes: Western blot - Anti-Tau antibody [EPR22524-95] (Anti-Tau antibody [EPR22524-95] ab254256) at 1/1000 dilution
Lane 1: 293T cells transfected with an empty vector containing a flag tag whole cell lysate at 20 µg
Lane 2: 293T cells transfected with a human Tau expression vector containing a flag whole cell lysate at 20 µg
Lane 3: 293T cells transfected with a human MAP2 expression vector containing a flag whole cell lysate at 20 µg
Lane 4: 293T cells transfected with a human MAP4 expression vector containing a flag whole cell lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 78 kDa
Observed band size: 55-100 kDa
Exposure time: 1s
![Western blot - Anti-Tau antibody [EPR22524-95] - BSA and Azide free (ab255271), expandable thumbnail](https://content.abcam.com/products/images/tau-antibody-epr22524-95-bsa-and-azide-free-ab255271--western-blot-img424819.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Tau antibody [EPR22524-95] - BSA and Azide free (ab255271)")
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