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Mouse Recombinant Monoclonal TAU antibody. Carrier free. Suitable for WB, IHC-P and reacts with Human, Mouse, Rat samples.

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Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau antibody [EPR22524-95] - Mouse IgG1 (Chimeric) - BSA and Azide free (AB317842), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau antibody [EPR22524-95] - Mouse IgG1 (Chimeric) - BSA and Azide free (AB317842), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau antibody [EPR22524-95] - Mouse IgG1 (Chimeric) - BSA and Azide free (AB317842), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau antibody [EPR22524-95] - Mouse IgG1 (Chimeric) - BSA and Azide free (AB317842), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau antibody [EPR22524-95] - Mouse IgG1 (Chimeric) - BSA and Azide free (AB317842), expandable thumbnail

Key facts

Isotype

IgG1

Host species

Mouse

Storage buffer

pH: 7.2 - 7.4
Constituents: 100% PBS

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIHC-PIHC-FrIP
Human
Tested
Tested
Not recommended
Not recommended
Mouse
Tested
Tested
Not recommended
Not recommended
Rat
Tested
Tested
Not recommended
Not recommended

Tested
Tested

Species

Human, Mouse, Rat

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

-

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species

Mouse

Dilution info

-

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species

Rat

Dilution info

-

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species

Mouse, Human, Rat

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Human, Mouse, Rat

Dilution info

-

Notes

-

Target data

Function

Promotes microtubule assembly and stability, and might be involved in the establishment and maintenance of neuronal polarity. The C-terminus binds axonal microtubules while the N-terminus binds neural plasma membrane components, suggesting that tau functions as a linker protein between both. Axonal polarity is predetermined by tau localization (in the neuronal cell) in the domain of the cell body defined by the centrosome. The short isoforms allow plasticity of the cytoskeleton whereas the longer isoforms may preferentially play a role in its stabilization.

Alternative names

Microtubule-associated protein tau, Neurofibrillary tangle protein, Paired helical filament-tau, PHF-tau, Tau, Mtapt, Mapt

Recommended products

Mouse Recombinant Monoclonal TAU antibody. Carrier free. Suitable for WB, IHC-P and reacts with Human, Mouse, Rat samples.

Alternative names

Microtubule-associated protein tau, Neurofibrillary tangle protein, Paired helical filament-tau, PHF-tau, Tau, Mtapt, Mapt

Key facts

Isotype

IgG1

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free

Yes

Clone number

EPR22524-95

Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate long-term storage conditions

+4°C

Notes

This mouse monoclonal chimeric antibody (ab317842) is the carrier-free version of Anti-Tau antibody [EPR22524-95] - Mouse IgG1 (Chimeric) ab317841.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Tau also known as microtubule-associated protein Tau (MAPT) plays an important role in stabilizing microtubules in neuronal cells. Tau is primarily found in the central nervous system but also exists in peripheral neurons. Human Tau protein comes in six isoforms due to alternative splicing with molecular weights ranging from 48 kDa to 67 kDa. This protein predominantly locates in the axons of neurons where it maintains the stability of microtubule tracks necessary for axonal transport.

Biological function summary

Tau is involved in the assembly and stabilization of microtubules essential for maintaining neuronal structure. It interacts with microtubule-binding domains (MBD) to bind and bundle microtubules facilitating intracellular transport. Tau forms a part of the neuronal cytoskeleton complex working closely with other cytoskeletal proteins to preserve the proper axonal transport and function. Abnormally phosphorylated Tau often termed phospho-Tau disrupts this complex affecting microtubule stability.

Pathways

Tau has critical involvement in several signaling cascades such as the microtubule-binding and transport pathways. Glycogen synthase kinase 3 beta (GSK3β) and cyclin-dependent kinase 5 (CDK5) frequently phosphorylate Tau controlling its interaction with microtubules. Phosphorylated Tau accumulates leading to the formation of neurofibrillary tangles often observed in neurodegenerative conditions. Additionally Tau interacts with GAPDH impacting cellular energy regulation through potential pathway cross-talk involving oxidative stress responses.

Associated diseases and disorders

Tau is closely associated with Alzheimer's disease and frontotemporal dementia. In Alzheimer's disease hyperphosphorylated Tau aggregates into paired helical filaments forming neurofibrillary tangles while similar aggregates are observed in frontotemporal dementia. In these conditions Tau links to amyloid precursor protein (APP) where misregulated phosphorylation-driven interactions contribute to neurodegeneration. Identifying phospho-Tau and its altered interactions with related proteins aids in understanding and potentially treating these disorders.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

9 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau antibody [EPR22524-95] - Mouse IgG1 (Chimeric) - BSA and Azide free (ab317842), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau antibody [EPR22524-95] - Mouse IgG1 (Chimeric) - BSA and Azide free (ab317842)

    This data was developed using Anti-Tau antibody [EPR22524-95] - Mouse IgG1 (Chimeric) ab317841, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labeling Tau with Anti-Tau antibody [EPR22524-95] - Mouse IgG1 (Chimeric) ab317841 at 1/10000 (0.104 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining on human cerebral cortex.

    The section was incubated with Anti-Tau antibody [EPR22524-95] - Mouse IgG1 (Chimeric) ab317841 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau antibody [EPR22524-95] - Mouse IgG1 (Chimeric) - BSA and Azide free (ab317842), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau antibody [EPR22524-95] - Mouse IgG1 (Chimeric) - BSA and Azide free (ab317842)

    This data was developed using Anti-Tau antibody [EPR22524-95] - Mouse IgG1 (Chimeric) ab317841, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling Tau with Anti-Tau antibody [EPR22524-95] - Mouse IgG1 (Chimeric) ab317841 at 1/10000 (0.104 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining on mouse cerebral cortex.

    The section was incubated with Anti-Tau antibody [EPR22524-95] - Mouse IgG1 (Chimeric) ab317841 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau antibody [EPR22524-95] - Mouse IgG1 (Chimeric) - BSA and Azide free (ab317842), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau antibody [EPR22524-95] - Mouse IgG1 (Chimeric) - BSA and Azide free (ab317842)

    This data was developed using Anti-Tau antibody [EPR22524-95] - Mouse IgG1 (Chimeric) ab317841, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Rat cerebrum tissue labeling Tau with Anti-Tau antibody [EPR22524-95] - Mouse IgG1 (Chimeric) ab317841 at 1/10000 (0.104 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining on rat cerebral cortex.

    The section was incubated with Anti-Tau antibody [EPR22524-95] - Mouse IgG1 (Chimeric) ab317841 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau antibody [EPR22524-95] - Mouse IgG1 (Chimeric) - BSA and Azide free (ab317842), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau antibody [EPR22524-95] - Mouse IgG1 (Chimeric) - BSA and Azide free (ab317842)

    This data was developed using Anti-Tau antibody [EPR22524-95] - Mouse IgG1 (Chimeric) ab317841, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Mouse hippocampus tissue labeling Tau with Anti-Tau antibody [EPR22524-95] - Mouse IgG1 (Chimeric) ab317841 at 1/10000 (0.104 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining on mouse hippocampus.

    The section was incubated with Anti-Tau antibody [EPR22524-95] - Mouse IgG1 (Chimeric) ab317841 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau antibody [EPR22524-95] - Mouse IgG1 (Chimeric) - BSA and Azide free (ab317842), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau antibody [EPR22524-95] - Mouse IgG1 (Chimeric) - BSA and Azide free (ab317842)

    This data was developed using Anti-Tau antibody [EPR22524-95] - Mouse IgG1 (Chimeric) ab317841, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Rat hippocampus tissue labeling Tau with Anti-Tau antibody [EPR22524-95] - Mouse IgG1 (Chimeric) ab317841 at 1/10000 (0.104 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining on rat hippocampus.

    The section was incubated with Anti-Tau antibody [EPR22524-95] - Mouse IgG1 (Chimeric) ab317841 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau antibody [EPR22524-95] - Mouse IgG1 (Chimeric) - BSA and Azide free (ab317842), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau antibody [EPR22524-95] - Mouse IgG1 (Chimeric) - BSA and Azide free (ab317842)

    This data was developed using Anti-Tau antibody [EPR22524-95] - Mouse IgG1 (Chimeric) ab317841, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling Tau with Anti-Tau antibody [EPR22524-95] - Mouse IgG1 (Chimeric) ab317841 at 1/10000 (0.104 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Negative control: no staining on human liver.

    The section was incubated with Anti-Tau antibody [EPR22524-95] - Mouse IgG1 (Chimeric) ab317841 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau antibody [EPR22524-95] - Mouse IgG1 (Chimeric) - BSA and Azide free (ab317842), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau antibody [EPR22524-95] - Mouse IgG1 (Chimeric) - BSA and Azide free (ab317842)

    This data was developed using Anti-Tau antibody [EPR22524-95] - Mouse IgG1 (Chimeric) ab317841, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Mouse lliver tissue labeling Tau with Anti-Tau antibody [EPR22524-95] - Mouse IgG1 (Chimeric) ab317841 at 1/10000 (0.104 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Negative control: no staining on mouse liver.

    The section was incubated with Anti-Tau antibody [EPR22524-95] - Mouse IgG1 (Chimeric) ab317841 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau antibody [EPR22524-95] - Mouse IgG1 (Chimeric) - BSA and Azide free (ab317842), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau antibody [EPR22524-95] - Mouse IgG1 (Chimeric) - BSA and Azide free (ab317842)

    This data was developed using Anti-Tau antibody [EPR22524-95] - Mouse IgG1 (Chimeric) ab317841, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Rat liver tissue labeling Tau with Anti-Tau antibody [EPR22524-95] - Mouse IgG1 (Chimeric) ab317841 at 1/10000 (0.104 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Negative control: no staining on rat liver.

    The section was incubated with Anti-Tau antibody [EPR22524-95] - Mouse IgG1 (Chimeric) ab317841 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Western blot - Anti-Tau antibody [EPR22524-95] - Mouse IgG1 (Chimeric) - BSA and Azide free (ab317842), expandable thumbnail

    Western blot - Anti-Tau antibody [EPR22524-95] - Mouse IgG1 (Chimeric) - BSA and Azide free (ab317842)

    This data was developed using Anti-Tau antibody [EPR22524-95] - Mouse IgG1 (Chimeric) ab317841, the same antibody clone in a different buffer formulation.

    Negative control: liver, heart, kidney, spleen (PMID: 24386422).

    The identity of the lower band at approximately 15 kDa (in lane 1) is unknown.

    Lanes 1-3 blot were developed using a high sensitivity ECL substrate.

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    Exposure time: Lanes 1-4: 169 seconds; Lanes 5-12: 48 seconds

    All lanes: Western blot - Anti-Tau antibody [EPR22524-95] - Mouse IgG1 (Chimeric) (Anti-Tau antibody [EPR22524-95] - Mouse IgG1 (Chimeric) ab317841) at 1/1000 dilution

    Lane 1: Human cerebellum tissue lysate at 20 µg

    Lane 2: Human liver tissue lysate at 20 µg

    Lane 3: Human kidney tissue lysate at 20 µg

    Lane 4: Human spleen tissue lysate at 20 µg

    Lane 5: Mouse brain tissue lysate at 20 µg

    Lane 6: Mouse heart tissue lysate at 20 µg

    Lane 7: Mouse kidney tissue lysate at 20 µg

    Lane 8: Mouse spleen tissue lysate at 20 µg

    Lane 9: Rat brain tissue lysate at 20 µg

    Lane 10: Rat heart tissue lysate at 20 µg

    Lane 11: Rat kidney tissue lysate at 20 µg

    Lane 12: Rat spleen tissue lysate at 20 µg

    Secondary

    All lanes: Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

    Observed band size: 50-70 kDa, 36 kDa

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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