JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB109392

Anti-Tau antibody [EPR2396(2)]

2

(1 Review)

|

(7 Publications)

Rabbit Recombinant Monoclonal TAU antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 7 publications.

View Alternative Names

MAPTL, MTBT1, TAU, MAPT, Microtubule-associated protein tau, Neurofibrillary tangle protein, Paired helical filament-tau, PHF-tau, Big tau, MAPTL, MTBT1, TAU, MAPT, Microtubule-associated protein tau, Neurofibrillary tangle protein, Paired helical filament-tau, PHF-tau

4 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau antibody [EPR2396(2)] (AB109392)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau antibody [EPR2396(2)] (AB109392)

Immunohistochemical analysis of paraffin-embedded Human glioma tissue using 1/250 ab109392.

Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau antibody [EPR2396(2)] (AB109392)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau antibody [EPR2396(2)] (AB109392)

Immunohistochemical analysis of paraffin-embedded Human brain tissue using 1/250 ab109392.

Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.

Immunocytochemistry/ Immunofluorescence - Anti-Tau antibody [EPR2396(2)] (AB109392)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Tau antibody [EPR2396(2)] (AB109392)

Immunofluorescent staining of SHSY5Y cells using 1/100 ab109392.

Western blot - Anti-Tau antibody [EPR2396(2)] (AB109392)
  • WB

Supplier Data

Western blot - Anti-Tau antibody [EPR2396(2)] (AB109392)

Blocking and Diluting buffer and concentration : 5% NFDM/TBST

All lanes:

Western blot - Anti-Tau antibody [EPR2396(2)] (ab109392) at 1/1000 dilution

All lanes:

Human Hippocampus whole tissue lysate at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 123 kDa,13 kDa,153 kDa,19 kDa,34 kDa,35 kDa,43 kDa,48 kDa,56 kDa,68 kDa,71 kDa,74 kDa,76 kDa,78 kDa,94 kDa

Observed band size: 110 kDa,13 kDa,155 kDa,19 kDa,37 kDa,43 kDa,50-70 kDa,58 kDa,68 kDa,71 kDa,74 kDa,75 kDa,81 kDa

false

Exposure time: 1s

  • Carrier free

    Anti-Tau antibody [EPR2396(2)] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR2396(2)

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, IHC-P, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

The specificity of this antibody refers to P10636-8.

This antibody clone has been shown to react with Tau 1N and 2N isoforms and does not react with 0N isoforms (doi.org/10.1101/2023.04.13.536711)

style
left-column

Alternative Products

Primary Antibodies

AB92682

Anti-Tau (phospho Ser673) antibody [EPR2603]

20ul selling size|RabMAb|Recombinant

Western blot - Anti-Tau (phospho Ser673) antibody [EPR2603] (AB92682)

  • 0
  • 0
Primary Antibodies

AB109390

Anti-Tau (phospho S396) antibody [EPR2731]

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant

Immunohistochemistry (Frozen sections) - Anti-Tau (phospho S396) antibody [EPR2731] (AB109390)

  • 4
  • 13
Primary Antibodies

AB151559

Anti-Tau (phospho T231) antibody [EPR2488]

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (phospho T231) antibody [EPR2488] (AB151559)

  • 5
  • 6
Primary Antibodies

AB32057

Anti-Tau (phospho S396) antibody [E178]

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (phospho S396) antibody [E178] (AB32057)

  • 5
  • 5
Primary Antibodies

AB170892

Anti-Tau (phospho S214) antibody [EPR1884(2)]

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant|Trial Size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (phospho S214) antibody [EPR1884(2)] (AB170892)

  • 5
  • 1
Primary Antibodies

AB92676

Anti-Tau (phospho S404) antibody [EPR2605]

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (phospho S404) antibody [EPR2605] (AB92676)

  • 5
  • 4
Primary Antibodies

AB93726

Anti-Tau antibody [SP70]

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau antibody [SP70] (AB93726)

  • 5
  • 4

Complementary Products

Assay Kits

AB273617

Human Tau ELISA Kit

Recombinant|SimpleStep

ELISA - Human Tau ELISA Kit (AB273617)

  • 0
  • 0
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB68428

Anti-GFAP antibody [EPR1034Y] - Astrocyte Marker

BOND RX™ Validated|20ul selling size|RabMAb|Advanced Validation|Recombinant

Multiplex immunohistochemistry - Anti-GFAP antibody [EPR1034Y] - Astrocyte Marker (AB68428)

  • 5
  • 20
Primary Antibodies

AB32454

Anti-MAP2 antibody - Neuronal Marker

BOND RX™ Validated|Lab Essentials

Immunocytochemistry/ Immunofluorescence - Anti-MAP2 antibody - Neuronal Marker (AB32454)

  • 5
  • 35
Primary Antibodies

AB32136

Anti-Amyloid Precursor Protein antibody [Y188]

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Amyloid Precursor Protein antibody [Y188] (AB32136)

  • 4
  • 9
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/250 - 1/500", "IHCP-species-notes": "<p>Heat up to 98 degrees C, below boiling, and then let cool for 10-20 min.</p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/250", "ICCIF-species-notes": "<p></p>" } } }
style
left-column

Product details

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Stable for 12 months at -20°C
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Tau also known as microtubule-associated protein Tau (MAPT) plays an important role in stabilizing microtubules in neuronal cells. Tau is primarily found in the central nervous system but also exists in peripheral neurons. Human Tau protein comes in six isoforms due to alternative splicing with molecular weights ranging from 48 kDa to 67 kDa. This protein predominantly locates in the axons of neurons where it maintains the stability of microtubule tracks necessary for axonal transport.
Biological function summary

Tau is involved in the assembly and stabilization of microtubules essential for maintaining neuronal structure. It interacts with microtubule-binding domains (MBD) to bind and bundle microtubules facilitating intracellular transport. Tau forms a part of the neuronal cytoskeleton complex working closely with other cytoskeletal proteins to preserve the proper axonal transport and function. Abnormally phosphorylated Tau often termed phospho-Tau disrupts this complex affecting microtubule stability.

Pathways

Tau has critical involvement in several signaling cascades such as the microtubule-binding and transport pathways. Glycogen synthase kinase 3 beta (GSK3β) and cyclin-dependent kinase 5 (CDK5) frequently phosphorylate Tau controlling its interaction with microtubules. Phosphorylated Tau accumulates leading to the formation of neurofibrillary tangles often observed in neurodegenerative conditions. Additionally Tau interacts with GAPDH impacting cellular energy regulation through potential pathway cross-talk involving oxidative stress responses.

Tau is closely associated with Alzheimer's disease and frontotemporal dementia. In Alzheimer's disease hyperphosphorylated Tau aggregates into paired helical filaments forming neurofibrillary tangles while similar aggregates are observed in frontotemporal dementia. In these conditions Tau links to amyloid precursor protein (APP) where misregulated phosphorylation-driven interactions contribute to neurodegeneration. Identifying phospho-Tau and its altered interactions with related proteins aids in understanding and potentially treating these disorders.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Promotes microtubule assembly and stability, and might be involved in the establishment and maintenance of neuronal polarity (PubMed : 21985311). The C-terminus binds axonal microtubules while the N-terminus binds neural plasma membrane components, suggesting that tau functions as a linker protein between both (PubMed : 21985311, PubMed : 32961270). Axonal polarity is predetermined by TAU/MAPT localization (in the neuronal cell) in the domain of the cell body defined by the centrosome. The short isoforms allow plasticity of the cytoskeleton whereas the longer isoforms may preferentially play a role in its stabilization.
See full target information MAPT

Additional targets

MAPT
style
left-column

Publications (7)

Recent publications for all applications. Explore the full list and refine your search

Acta neuropathologica 149:2 PubMed39688618

2024

Amyloid-β oligomers increase the binding and internalization of tau oligomers in human synapses.

Applications

Unspecified application

Species

Unspecified reactive species

Shrinath Kadamangudi,Michela Marcatti,Wen-Ru Zhang,Anna Fracassi,Rakez Kayed,Agenor Limon,Giulio Taglialatela

Journal of molecular neuroscience : MN 74:105 PubMed39496982

2024

Sex Differences in Blood Accumulation of Neurodegenerative-Related Proteins and Antioxidant Responses to Regular Physical Exercise.

Applications

Unspecified application

Species

Unspecified reactive species

Elisa Chelucci,Giorgia Scarfò,Rebecca Piccarducci,Antonio Rizza,Jonathan Fusi,Francesco Epifani,Sara Carpi,Beatrice Polini,Laura Betti,Barbara Costa,Sabrina Taliani,Vito Cela,Paolo Artini,Simona Daniele,Claudia Martini,Ferdinando Franzoni

PloS one 19:e0312834 PubMed39475992

2024

SARS-CoV-2 propagation to the TPH2-positive neurons in the ventral tegmental area induces cell death via GSK3β-dependent accumulation of phosphorylated tau.

Applications

Unspecified application

Species

Unspecified reactive species

Motoki Imai,Fumitaka Kawakami,Takayuki Uematsu,Toshihide Matsumoto,Rei Kawashima,Yoshifumi Kurosaki,Shun Tamaki,Shotaro Maehana,Takafumi Ichikawa,Hideaki Hanaki,Hidero Kitazato,Makoto Kubo

Acta neuropathologica 147:87 PubMed38761203

2024

Identification of high-performing antibodies for the reliable detection of Tau proteoforms by Western blotting and immunohistochemistry.

Applications

Unspecified application

Species

Unspecified reactive species

Michael J Ellis,Christiana Lekka,Katie L Holden,Hanna Tulmin,Faheem Seedat,Darragh P O'Brien,Shalinee Dhayal,Marie-Louise Zeissler,Jakob G Knudsen,Benedikt M Kessler,Noel G Morgan,John A Todd,Sarah J Richardson,M Irina Stefana

Alzheimer's & dementia : the journal of the Alzheimer's Association 19:5355-5370 PubMed37191183

2023

Preserved autophagy in cognitively intact non-demented individuals with Alzheimer's neuropathology.

Applications

Unspecified application

Species

Unspecified reactive species

Batbayar Tumurbaatar,Anna Fracassi,Pietro Scaduto,Jutatip Guptarak,Randall Woltjer,Daniel Jupiter,Giulio Taglialatela

The European journal of neuroscience 56:4514-4528 PubMed35902984

2022

α-Glyceryl-phosphoryl-ethanolamine protects human hippocampal neurons from ageing-induced cellular alterations.

Applications

Unspecified application

Species

Unspecified reactive species

Elisa Zappelli,Simona Daniele,Lorenzo Ceccarelli,Matteo Vergassola,Lorella Ragni,Giorgina Mangano,Claudia Martini

Frontiers in pharmacology 12:748021 PubMed34658885

2021

Palmitoylethanolamide Counteracts Enteric Inflammation and Bowel Motor Dysfunctions in a Mouse Model of Alzheimer's Disease.

Applications

Unspecified application

Species

Unspecified reactive species

Vanessa D'Antongiovanni,Carolina Pellegrini,Luca Antonioli,Laura Benvenuti,Clelia Di Salvo,Lorenzo Flori,Rebecca Piccarducci,Simona Daniele,Alma Martelli,Vincenzo Calderone,Claudia Martini,Matteo Fornai
View all publications
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com