Anti-Tau antibody [PC1C6] - BSA and Azide free

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau antibody [PC1C6] - BSA and Azide free (AB255907)

Immunohistochemical analysis of paraffin-embedded human cerebrum tissue labeling Tau with ab254150 at 1/5000 dilution (0.154μg/ml) followed by ready to use Goat Anti-mouse IgG H&L (HRP polymer) (ab214879). Positive staining on human cerebrum. The section was incubated with ab254150 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is ready to use Goat Anti-mouse IgG H&L (HRP polymer) (ab214879).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab254150).

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau antibody [PC1C6] - BSA and Azide free (AB255907)

Immunohistochemical analysis of paraffin-embedded human glioma tissue labeling Tau with ab254150 at 1/5000 dilution (0.154μg/ml) followed by ready to use Goat Anti-mouse IgG H&L (HRP polymer) (ab214879). Positive staining on human glioma. The section was incubated with ab254150 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is ready to use Goat Anti-mouse IgG H&L (HRP polymer) (ab214879).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab254150).

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau antibody [PC1C6] - BSA and Azide free (AB255907)

Immunohistochemical analysis of paraffin-embedded rat hippocampus tissue labeling Tau with ab254150 at 1/10000 dilution (0.077μg/ml) followed by ready to use Goat Anti-mouse IgG H&L (HRP polymer) (ab214879). Positive staining on rat hippocampus. The section was incubated with ab254150 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is ready to use Goat Anti-mouse IgG H&L (HRP polymer) (ab214879).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab254150).

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau antibody [PC1C6] - BSA and Azide free (AB255907)

Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue labeling Tau with ab254150 at 1/10000 dilution (0.077μg/ml) followed by ready to use Goat Anti-mouse IgG H&L (HRP polymer) (ab214879). Positive staining on mouse cerebrum. The section was incubated with ab254150 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is ready to use Goat Anti-mouse IgG H&L (HRP polymer) (ab214879).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab254150).

• WB

Lab

Western blot - Anti-Tau antibody [PC1C6] - BSA and Azide free (AB255907)

Blocking/Dilution buffer : 5% NFDM/TBST.

The molecular weight observed is consistent with what has been described in the literature (PMID : 24386422 and 3930508).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab254150).

All lanes:

Western blot - Anti-Tau antibody [PC1C6] (<a href='/en-us/products/primary-antibodies/tau-antibody-pc1c6-ab254150'>ab254150</a>) at 0.77 µg/mL

Lane 1:

Human brain tissue lysate at 10 µg

Lane 2:

Human hippocampus tissue lysate at 10 µg

Lane 3:

Mouse brain tissue lysate at 10 µg

Lane 4:

Mouse hippocampus tissue lysate at 10 µg

Lane 5:

Rat hippocampus tissue lysate at 10 µg

Secondary

All lanes:

Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

Predicted band size: 78 kDa

Observed band size: 37-70 kDa

false

Exposure time: 3s