Mouse Recombinant Monoclonal TAU antibody. Carrier free. Suitable for WB, IHC-P and reacts with Mouse, Rat, Human samples. Cited in 1 publication.
Images
![Western blot - Anti-Tau antibody [PC1C6] - BSA and Azide free (AB255907), expandable thumbnail](https://content.abcam.com/products/images/tau-antibody-pc1c6-bsa-and-azide-free-ab255907--western-blot-img54823.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Tau antibody [PC1C6] - BSA and Azide free (AB255907)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau antibody [PC1C6] - BSA and Azide free (AB255907), expandable thumbnail](https://content.abcam.com/products/images/tau-antibody-pc1c6-bsa-and-azide-free-ab255907--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img28206.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau antibody [PC1C6] - BSA and Azide free (AB255907)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau antibody [PC1C6] - BSA and Azide free (AB255907), expandable thumbnail](https://content.abcam.com/products/images/tau-antibody-pc1c6-bsa-and-azide-free-ab255907--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img121366.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau antibody [PC1C6] - BSA and Azide free (AB255907)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau antibody [PC1C6] - BSA and Azide free (AB255907), expandable thumbnail](https://content.abcam.com/products/images/tau-antibody-pc1c6-bsa-and-azide-free-ab255907--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img135649.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau antibody [PC1C6] - BSA and Azide free (AB255907)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau antibody [PC1C6] - BSA and Azide free (AB255907), expandable thumbnail](https://content.abcam.com/products/images/tau-antibody-pc1c6-bsa-and-azide-free-ab255907--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img87643.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau antibody [PC1C6] - BSA and Azide free (AB255907)")
Publications
- The Journal of cell biology 101:1371-81985The distribution of tau in the mammalian central nervous system.Applications:Unspecified applicationReactive species:Unspecified reactive speciesL I Binder,A Frankfurter,L I RebhunPubMed 3930508
Key facts
- Isotype
IgG2a
- Host species
Mouse
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
Liquid
- Clonality
Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | ICC/IF | IHC-P | |
|---|---|---|---|
| Human | Tested | Not recommended | Tested |
| Mouse | Tested | Not recommended | Tested |
| Rat | Tested | Not recommended | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Rat | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Rat | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Associated Products
Select an associated product type
1 products for Alternative Version
Target data
Function
Promotes microtubule assembly and stability, and might be involved in the establishment and maintenance of neuronal polarity. The C-terminus binds axonal microtubules while the N-terminus binds neural plasma membrane components, suggesting that tau functions as a linker protein between both. Axonal polarity is predetermined by tau localization (in the neuronal cell) in the domain of the cell body defined by the centrosome. The short isoforms allow plasticity of the cytoskeleton whereas the longer isoforms may preferentially play a role in its stabilization.
Alternative names
Microtubule-associated protein tau, Neurofibrillary tangle protein, Paired helical filament-tau, PHF-tau, MAPT, TAU
Recommended products
Mouse Recombinant Monoclonal TAU antibody. Carrier free. Suitable for WB, IHC-P and reacts with Mouse, Rat, Human samples. Cited in 1 publication.
Alternative names
Microtubule-associated protein tau, Neurofibrillary tangle protein, Paired helical filament-tau, PHF-tau, MAPT, TAU
Key facts
- Isotype
IgG2a
- Host species
Mouse
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
Yes
- Clone number
PC1C6
- Purification technique
Affinity purification Protein A
- Specificity
The immunogen of this antibody is from cow Tau, but we haven't tested this antibody with cow samples.
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
+4°C
- Storage information
Do Not Freeze
Notes
ab255907 is the carrier-free version of Anti-Tau antibody [PC1C6] ab254150.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- Activity summary
Tau also known as microtubule-associated protein Tau (MAPT) plays an important role in stabilizing microtubules in neuronal cells. Tau is primarily found in the central nervous system but also exists in peripheral neurons. Human Tau protein comes in six isoforms due to alternative splicing with molecular weights ranging from 48 kDa to 67 kDa. This protein predominantly locates in the axons of neurons where it maintains the stability of microtubule tracks necessary for axonal transport.
- Biological function summary
Tau is involved in the assembly and stabilization of microtubules essential for maintaining neuronal structure. It interacts with microtubule-binding domains (MBD) to bind and bundle microtubules facilitating intracellular transport. Tau forms a part of the neuronal cytoskeleton complex working closely with other cytoskeletal proteins to preserve the proper axonal transport and function. Abnormally phosphorylated Tau often termed phospho-Tau disrupts this complex affecting microtubule stability.
- Pathways
Tau has critical involvement in several signaling cascades such as the microtubule-binding and transport pathways. Glycogen synthase kinase 3 beta (GSK3β) and cyclin-dependent kinase 5 (CDK5) frequently phosphorylate Tau controlling its interaction with microtubules. Phosphorylated Tau accumulates leading to the formation of neurofibrillary tangles often observed in neurodegenerative conditions. Additionally Tau interacts with GAPDH impacting cellular energy regulation through potential pathway cross-talk involving oxidative stress responses.
- Associated diseases and disorders
Tau is closely associated with Alzheimer's disease and frontotemporal dementia. In Alzheimer's disease hyperphosphorylated Tau aggregates into paired helical filaments forming neurofibrillary tangles while similar aggregates are observed in frontotemporal dementia. In these conditions Tau links to amyloid precursor protein (APP) where misregulated phosphorylation-driven interactions contribute to neurodegeneration. Identifying phospho-Tau and its altered interactions with related proteins aids in understanding and potentially treating these disorders.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
5 product images
![Western blot - Anti-Tau antibody [PC1C6] - BSA and Azide free (ab255907), expandable thumbnail](https://content.abcam.com/products/images/tau-antibody-pc1c6-bsa-and-azide-free-ab255907--western-blot-img54823.jpg "Western blot - Anti-Tau antibody [PC1C6] - BSA and Azide free (ab255907)")
Western blot - Anti-Tau antibody [PC1C6] - BSA and Azide free (ab255907)
Blocking/Dilution buffer: 5% NFDM/TBST.
The molecular weight observed is consistent with what has been described in the literature (PMID: 24386422 and 3930508).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Tau antibody [PC1C6] ab254150).
All lanes: Western blot - Anti-Tau antibody [PC1C6] (Anti-Tau antibody [PC1C6] ab254150) at 0.77 µg/mL
Lane 1: Human brain tissue lysate at 10 µg
Lane 2: Human hippocampus tissue lysate at 10 µg
Lane 3: Mouse brain tissue lysate at 10 µg
Lane 4: Mouse hippocampus tissue lysate at 10 µg
Lane 5: Rat hippocampus tissue lysate at 10 µg
SecondaryAll lanes: Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution
Predicted band size: 78 kDa
Observed band size: 37-70 kDa
Exposure time: 3s
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau antibody [PC1C6] - BSA and Azide free (ab255907), expandable thumbnail](https://content.abcam.com/products/images/tau-antibody-pc1c6-bsa-and-azide-free-ab255907--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img28206.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau antibody [PC1C6] - BSA and Azide free (ab255907)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau antibody [PC1C6] - BSA and Azide free (ab255907)
Immunohistochemical analysis of paraffin-embedded human cerebrum tissue labeling Tau with Anti-Tau antibody [PC1C6] ab254150 at 1/5000 dilution (0.154μg/ml) followed by ready to use Goat Anti-mouse IgG H&L (HRP polymer) (Goat Anti-Mouse IgG H&L (HRP polymer) ab214879). Positive staining on human cerebrum. The section was incubated with Anti-Tau antibody [PC1C6] ab254150 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is ready to use Goat Anti-mouse IgG H&L (HRP polymer) (Goat Anti-Mouse IgG H&L (HRP polymer) ab214879).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Tau antibody [PC1C6] ab254150).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau antibody [PC1C6] - BSA and Azide free (ab255907), expandable thumbnail](https://content.abcam.com/products/images/tau-antibody-pc1c6-bsa-and-azide-free-ab255907--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img121366.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau antibody [PC1C6] - BSA and Azide free (ab255907)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau antibody [PC1C6] - BSA and Azide free (ab255907)
Immunohistochemical analysis of paraffin-embedded human glioma tissue labeling Tau with Anti-Tau antibody [PC1C6] ab254150 at 1/5000 dilution (0.154μg/ml) followed by ready to use Goat Anti-mouse IgG H&L (HRP polymer) (Goat Anti-Mouse IgG H&L (HRP polymer) ab214879). Positive staining on human glioma. The section was incubated with Anti-Tau antibody [PC1C6] ab254150 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is ready to use Goat Anti-mouse IgG H&L (HRP polymer) (Goat Anti-Mouse IgG H&L (HRP polymer) ab214879).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Tau antibody [PC1C6] ab254150).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau antibody [PC1C6] - BSA and Azide free (ab255907), expandable thumbnail](https://content.abcam.com/products/images/tau-antibody-pc1c6-bsa-and-azide-free-ab255907--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img135649.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau antibody [PC1C6] - BSA and Azide free (ab255907)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau antibody [PC1C6] - BSA and Azide free (ab255907)
Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue labeling Tau with Anti-Tau antibody [PC1C6] ab254150 at 1/10000 dilution (0.077μg/ml) followed by ready to use Goat Anti-mouse IgG H&L (HRP polymer) (Goat Anti-Mouse IgG H&L (HRP polymer) ab214879). Positive staining on mouse cerebrum. The section was incubated with Anti-Tau antibody [PC1C6] ab254150 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is ready to use Goat Anti-mouse IgG H&L (HRP polymer) (Goat Anti-Mouse IgG H&L (HRP polymer) ab214879).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Tau antibody [PC1C6] ab254150).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau antibody [PC1C6] - BSA and Azide free (ab255907), expandable thumbnail](https://content.abcam.com/products/images/tau-antibody-pc1c6-bsa-and-azide-free-ab255907--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img87643.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau antibody [PC1C6] - BSA and Azide free (ab255907)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau antibody [PC1C6] - BSA and Azide free (ab255907)
Immunohistochemical analysis of paraffin-embedded rat hippocampus tissue labeling Tau with Anti-Tau antibody [PC1C6] ab254150 at 1/10000 dilution (0.077μg/ml) followed by ready to use Goat Anti-mouse IgG H&L (HRP polymer) (Goat Anti-Mouse IgG H&L (HRP polymer) ab214879). Positive staining on rat hippocampus. The section was incubated with Anti-Tau antibody [PC1C6] ab254150 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is ready to use Goat Anti-mouse IgG H&L (HRP polymer) (Goat Anti-Mouse IgG H&L (HRP polymer) ab214879).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Tau antibody [PC1C6] ab254150).
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com