Anti-Tau antibody [TAU-5] - BSA and Azide free (ab80579) is a mouse recombinant monoclonal antibody provided in a PBS only buffer for easy conjugation detecting Tau in Western Blot, ICC/IF. Suitable for Human, Mouse, Rat.
- BSA, sodium azide, and glycerol-free for easy conjugation
- Biophysical QC for unrivalled batch-batch consistency
- Over 150 publications
- Trusted since 2009
Images
![Western blot - Anti-Tau antibody [TAU-5] - BSA and Azide free (AB80579), expandable thumbnail](https://content.abcam.com/products/images/tau-antibody-tau-5-bsa-and-azide-free-ab80579--western-blot-img408707.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Tau antibody [TAU-5] - BSA and Azide free (AB80579)")
![Immunocytochemistry/ Immunofluorescence - Anti-Tau antibody [TAU-5] - BSA and Azide free (AB80579), expandable thumbnail](https://content.abcam.com/products/images/tau-antibody-tau-5-bsa-and-azide-free-ab80579--immunocytochemistry-immunofluorescence-img408706.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-Tau antibody [TAU-5] - BSA and Azide free (AB80579)")
Publications
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- Aging 15:14172-141912023Gypenoside IX restores Akt/GSK-3β pathway and alleviates Alzheimer's disease-like neuropathology and cognitive deficits.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLing Lei,Yong Luo,Dongkun Kang,Fumin Yang,Dongli Meng,Jian-Zhi Wang,Rong Liu,Xiaochuan Wang,Hong-Lian LiPubMed 38095632
- Acta neuropathologica communications 11:1662023Antisense oligonucleotide-based targeting of Tau-tubulin kinase 1 prevents hippocampal accumulation of phosphorylated tau in PS19 tauopathy mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKayo Yukawa,Satomi Yamamoto-Mcguire,Louis Cafaro,Christine Hong,Fredrik Kamme,Tsuneya Ikezu,Seiko IkezuPubMed 37853497
- Journal of neurochemistry 167:441-4602023Effects of anti-tau immunotherapy on reactive microgliosis, cerebral endotheliopathy, and cognitive function in an experimental model of cerebral malaria.Applications:Unspecified applicationReactive species:Unspecified reactive speciesOscar B Akide Ndunge,Henry J Shikani,Minxian Dai,Brandi D Freeman,Mahalia S DesruisseauxPubMed 37814468
- Signal transduction and targeted therapy 8:3582023Tauopathy promotes spinal cord-dependent production of toxic amyloid-beta in transgenic monkeys.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZhuchi Tu,Sen Yan,Bofeng Han,Caijuan Li,Weien Liang,Yingqi Lin,Yongyan Ding,Huiyi Wei,Lu Wang,Hao Xu,Jianmeng Ye,Bang Li,Shihua Li,Xiao-Jiang LiPubMed 37735155
- PNAS nexus 2:pgad2822023Severe acute respiratory syndrome coronavirus 2 infection leads to Tau pathological signature in neurons.Applications:Unspecified applicationReactive species:Unspecified reactive speciesCristina Di Primio,Paola Quaranta,Marianna Mignanelli,Giacomo Siano,Matteo Bimbati,Arianna Scarlatti,Carmen Rita Piazza,Piero Giorgio Spezia,Paola Perrera,Fulvio Basolo,Anello Marcello Poma,Mario Costa,Mauro Pistello,Antonino CattaneoPubMed 37731949
- Journal of extracellular vesicles 12:e123582023Comprehensive characterization of human brain-derived extracellular vesicles using multiple isolation methods: Implications for diagnostic and therapeutic applications.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZhengrong Zhang,Kaiwen Yu,Yang You,Peizhou Jiang,Zhiping Wu,Michael A DeTure,Dennis W Dickson,Seiko Ikezu,Junmin Peng,Tsuneya IkezuPubMed 37563857
- International journal of biological sciences 19:2409-24272023Brain-derived extracellular vesicles promote bone-fat imbalance in Alzheimer's disease.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXixi Liu,Chunyuan Chen,Yaling Jiang,Meidan Wan,Bin Jiao,Xinxin Liao,Shanshan Rao,Chungu Hong,Qijie Yang,Yuan Zhu,Qianqian Liu,Zhongwei Luo,Ran Duan,Yiyi Wang,Yijuan Tan,Jia Cao,Zhengzhao Liu,Zhenxing Wang,Hui Xie,Lu ShenPubMed 37215980
- iScience 26:1066742023Mitochondrial Bcl-xL promotes brain synaptogenesis by controlling non-lethal caspase activation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTrang Thi Minh Nguyen,Rudy Gadet,Marine Lanfranchi,Romane A Lahaye,Sozerko Yandiev,Olivier Lohez,Ivan Mikaelian,Lea Jabbour,Ruth Rimokh,Julien Courchet,Frédéric Saudou,Nikolay Popgeorgiev,Germain GilletPubMed 37182099
- Molecular neurodegeneration 18:232023Targeting a vulnerable septum-hippocampus cholinergic circuit in a critical time window ameliorates tau-impaired memory consolidation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDongqin Wu,Nana Yu,Yang Gao,Rui Xiong,Luping Liu,Huiyang Lei,Sen Jin,Jiale Liu,Yingzhou Liu,Jiazhao Xie,Enjie Liu,Qiuzhi Zhou,Yanchao Liu,Shihong Li,Linyu Wei,Jingru Lv,Huilin Yu,Wenbo Zeng,Qiang Zhou,Fuqiang Xu,Min-Hua Luo,Yao Zhang,Ying Yang,Jian-Zhi WangPubMed 37060096
- Acta neuropathologica communications 11:612023Exogenous interleukin 33 enhances the brain's lymphatic drainage and toxic protein clearance in acute traumatic brain injury mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMingqi Liu,Jinhao Huang,Tao Liu,Jiangyuan Yuan,Chuanxiang Lv,Zhuang Sha,Chenrui Wu,Weiwei Jiang,Xuanhui Liu,Meng Nie,Yupeng Chen,Shiying Dong,Yu Qian,Chuang Gao,Yibing Fan,Di Wu,Rongcai JiangPubMed 37024941
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
Constituents: 100% PBS
- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | ICC/IF | |
|---|---|---|
| Human | Tested | Expected |
| Mouse | Tested | Expected |
| Rat | Tested | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1 µg/mL | Notes - |
Species Rat | Dilution info 1 µg/mL | Notes - |
Species Human | Dilution info 1 µg/mL | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info 1 µg/mL | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
Select an associated product type
9 products for Alternative Product
Target data
Function
Promotes microtubule assembly and stability, and might be involved in the establishment and maintenance of neuronal polarity. The C-terminus binds axonal microtubules while the N-terminus binds neural plasma membrane components, suggesting that tau functions as a linker protein between both. Axonal polarity is predetermined by tau localization (in the neuronal cell) in the domain of the cell body defined by the centrosome. The short isoforms allow plasticity of the cytoskeleton whereas the longer isoforms may preferentially play a role in its stabilization.
Alternative names
TAU, MAPT, Microtubule-associated protein tau, Neurofibrillary tangle protein, Paired helical filament-tau, PHF-tau
Recommended products
Anti-Tau antibody [TAU-5] - BSA and Azide free (ab80579) is a mouse recombinant monoclonal antibody provided in a PBS only buffer for easy conjugation detecting Tau in Western Blot, ICC/IF. Suitable for Human, Mouse, Rat.
- BSA, sodium azide, and glycerol-free for easy conjugation
- Biophysical QC for unrivalled batch-batch consistency
- Over 150 publications
- Trusted since 2009
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
Constituents: 100% PBS
- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- TAU-5
- Purification technique
- Affinity purification Protein G
- Specificity
The specificity of this antibody refers to P29172.
- Epitope
- The epitope has been mapped to the Human tau sequence 218-225 (doi:10.1016/j.bbrc.2007.04.187)
- Light chain type
- kappa
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Anti-Tau antibody [TAU-5] - BSA and Azide free (ab80579) is a mouse recombinant monoclonal antibody and is provided in a carrier-free, conjugation ready format. ab80579 is validated in ICC/IF, WBin human, mouse, rat samples. Carrier-free antibodies are compatible with our best-in-class range of Lightning-Link® conjugation kits.
Anti-Tau antibody [TAU-5] - BSA and Azide free (ab80579) specifically detects Tau (UniProt ID: P29172; Molecular weight: 46kDa) and is sold in 100 µg selling sizes.
Quality and Validation
Antibody clone TAU-5 has been cited over 150 times in peer reviewed journals and is trusted by the scientific community.
Anti-Tau antibody [TAU-5] - BSA and Azide free (ab80579) has 5 independent reviews from customers.
Browse our curated portfolio of extensively validated recombinant antibodies and assays sensitive to Tau, Beta-amyloid, and other Alzheimer’s targets of interest, with everything you need in one place.
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Tau also known as microtubule-associated protein Tau (MAPT) plays an important role in stabilizing microtubules in neuronal cells. Tau is primarily found in the central nervous system but also exists in peripheral neurons. Human Tau protein comes in six isoforms due to alternative splicing with molecular weights ranging from 48 kDa to 67 kDa. This protein predominantly locates in the axons of neurons where it maintains the stability of microtubule tracks necessary for axonal transport.
- Biological function summary
Tau is involved in the assembly and stabilization of microtubules essential for maintaining neuronal structure. It interacts with microtubule-binding domains (MBD) to bind and bundle microtubules facilitating intracellular transport. Tau forms a part of the neuronal cytoskeleton complex working closely with other cytoskeletal proteins to preserve the proper axonal transport and function. Abnormally phosphorylated Tau often termed phospho-Tau disrupts this complex affecting microtubule stability.
- Pathways
Tau has critical involvement in several signaling cascades such as the microtubule-binding and transport pathways. Glycogen synthase kinase 3 beta (GSK3β) and cyclin-dependent kinase 5 (CDK5) frequently phosphorylate Tau controlling its interaction with microtubules. Phosphorylated Tau accumulates leading to the formation of neurofibrillary tangles often observed in neurodegenerative conditions. Additionally Tau interacts with GAPDH impacting cellular energy regulation through potential pathway cross-talk involving oxidative stress responses.
- Associated diseases and disorders
Tau is closely associated with Alzheimer's disease and frontotemporal dementia. In Alzheimer's disease hyperphosphorylated Tau aggregates into paired helical filaments forming neurofibrillary tangles while similar aggregates are observed in frontotemporal dementia. In these conditions Tau links to amyloid precursor protein (APP) where misregulated phosphorylation-driven interactions contribute to neurodegeneration. Identifying phospho-Tau and its altered interactions with related proteins aids in understanding and potentially treating these disorders.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
![Western blot - Anti-Tau antibody [TAU-5] - BSA and Azide free (ab80579), expandable thumbnail](https://content.abcam.com/products/images/tau-antibody-tau-5-bsa-and-azide-free-ab80579--western-blot-img408707.jpg "Western blot - Anti-Tau antibody [TAU-5] - BSA and Azide free (ab80579)")
Western blot - Anti-Tau antibody [TAU-5] - BSA and Azide free (ab80579)
Overnight at 4єC. Binding at expected molecular weights consistent with Tau protein across the various species.
All lanes: Western blot - Anti-Tau antibody [TAU-5] - BSA and Azide free (ab80579) at 1 µg/mL
Lane 1: Human Brain Tissue Lysate at 40 µg with Milk in TBS-T (0.1% Tween)
Lane 2: Mouse Brain Tissue Lysate at 40 µg with Milk in TBS-T (0.1% Tween)
Lane 3: Rat Brain Tissue Lysate at 20 µg with Milk in TBS-T (0.1% Tween)
SecondaryLanes 1 - 3: Western blot - Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed ab216772) at 1/20000 dilution
Lanes 1 - 3: Western blot - Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed ab216777) at 1/20000 dilution
![Immunocytochemistry/ Immunofluorescence - Anti-Tau antibody [TAU-5] - BSA and Azide free (ab80579), expandable thumbnail](https://content.abcam.com/products/images/tau-antibody-tau-5-bsa-and-azide-free-ab80579--immunocytochemistry-immunofluorescence-img408706.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Tau antibody [TAU-5] - BSA and Azide free (ab80579)")
Immunocytochemistry/ Immunofluorescence - Anti-Tau antibody [TAU-5] - BSA and Azide free (ab80579)
Cells incubated overnight at 4°C. The cells were fixed with 4% paraformaldehyde (10 min), permeabilized with 0.1% PBS-Tween for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h.
Nuclear DNA was labelled with DAPI (shown in blue). Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.
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