Anti-Tau - C-terminal (4R-aa396) antibody [EPR29542-63]

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau - C-terminal (4R-aa396) antibody [EPR29542-63] (AB323709)

Immunohistochemical analysis of paraffin-embedded Human Alzheimer's disease cerebrum tissue labeling Tau - C-terminal (4R-aa396) with ab323709 at 1/2000 (0.243 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on human Alzheimer's disease cerebrum. The section was incubated with ab323709 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau - C-terminal (4R-aa396) antibody [EPR29542-63] (AB323709)

Immunohistochemical analysis of paraffin-embedded Human spleen tissue labeling Tau - C-terminal (4R-aa396) with ab323709 at 1/2000 (0.243 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Negative control : no staining on human spleen. The section was incubated with ab323709 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau - C-terminal (4R-aa396) antibody [EPR29542-63] (AB323709)

Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labeling Tau - C-terminal (4R-aa396) with ab323709 at 1/2000 (0.243 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on human cerebrum. The section was incubated with ab323709 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau - C-terminal (4R-aa396) antibody [EPR29542-63] (AB323709)

Immunohistochemical analysis of paraffin-embedded Mouse Alzheimer's disease cerebrum tissue labeling Tau - C-terminal (4R-aa396) with ab323709 at 1/2000 (0.243 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on mouse Alzheimer's disease cerebrum. The section was incubated with ab323709 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau - C-terminal (4R-aa396) antibody [EPR29542-63] (AB323709)

Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue labeling Tau - C-terminal (4R-aa396) with ab323709 at 1/2000 (0.243 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Negative control : no staining on mouse spleen (PMID : 24386422). The section was incubated with ab323709 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau - C-terminal (4R-aa396) antibody [EPR29542-63] (AB323709)

Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling Tau - C-terminal (4R-aa396) with ab323709 at 1/2000 (0.243 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on mouse cerebrum. The section was incubated with ab323709 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• WB

Supplier Data

Western blot - Anti-Tau - C-terminal (4R-aa396) antibody [EPR29542-63] (AB323709)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Negative control : liver, spleen (PMID : 24386422, PMID : 8752131)

The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID : 24116116, PMID : 8752131, PMID : 24386422, PMID : 37443334).

The band around 15kDa in lane 1 might be cleavage fragment of Tau (PMID : 37905868).

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

Exposure time : Lane1 : 125 seconds; Lanes 2-4 : 6 seconds

All lanes:

Western blot - Anti-Tau - C-terminal (4R-aa396) antibody [EPR29542-63] (ab323709) at 1/1000 dilution

Lane 1:

Human Alzheimers brain tissue lysate at 20 µg

Lane 2:

Human hippocampus tissue lysate at 20 µg

Lane 3:

Human spleen tissue lysate at 20 µg

Lane 4:

Human liver tissue lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Observed band size: 33-62 kDa,36 kDa

false

• WB

Supplier Data

Western blot - Anti-Tau - C-terminal (4R-aa396) antibody [EPR29542-63] (AB323709)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Negative control : liver, spleen, testis (PMID : 24386422, PMID : 8752131)

The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID : 24116116, PMID : 8752131, PMID : 24386422, PMID : 37443334).

The identity of the higher MW band at approximately 100 kDa in lane 7 is unknown.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-Tau - C-terminal (4R-aa396) antibody [EPR29542-63] (ab323709) at 1/1000 dilution

Lane 1:

Mouse Alzheimers brain tissue lysate at 20 µg

Lane 2:

Mouse brain tissue lysate at 20 µg

Lane 3:

Mouse hippocampus tissue lysate at 20 µg

Lane 4:

Mouse cerebellum tissue lysate at 20 µg

Lane 5:

Mouse spleen tissue lysate at 20 µg

Lane 6:

Mouse liver tissue lysate at 20 µg

Lane 7:

Mouse testis tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 33-62 kDa,36 kDa

false

Exposure time: 37s

• I-ELISA

Supplier Data

Indirect ELISA - Anti-Tau - C-terminal (4R-aa396) antibody [EPR29542-63] (AB323709)

Indirect ELISA analysis of ab323709 at 1000-0 ng/ml. The Secondary antibody used was Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1 : 2500 dilution dilution.

Antigen : Mouse PNS-Tau recombinant protein, Mouse Tau-A (corresponding to Human 2N4R) recombinant protein.

Antigen concentration : 1000 ng/ml

• I-ELISA

Supplier Data

Indirect ELISA - Anti-Tau - C-terminal (4R-aa396) antibody [EPR29542-63] (AB323709)

Indirect ELISA analysis of ab323709 at 1000-0 ng/ml. The Secondary antibody used was Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1 : 2500 dilution dilution.

Antigen : Human Tau 2N4R peptide (within 4R-aa396), Human Tau 2N4R recombinant protein, Human MAP2 fragment.

Antigen concentration : 1000 ng/ml

This antibody does not cross-react with human MAP2.