Anti-Tau - C-terminal (4R-aa396) antibody [EPR29542-63] - BSA and Azide free (ab324110)

Rabbit Recombinant Monoclonal TAU antibody. Carrier free. Suitable for I-ELISA, IHC-P, WB and reacts with Recombinant fragment - Mouse, Synthetic peptide - Human, Human, Mouse samples.

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Images

Western blot - Anti-Tau - C-terminal (4R-aa396) antibody [EPR29542-63] - BSA and Azide free (AB324110), expandable thumbnail

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Constituents: 100% PBS
Form: Liquid
Clonality: Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	I-ELISA	IHC-P	WB
Human	Expected	Tested	Tested
Mouse	Expected	Tested	Tested
Recombinant fragment - Mouse	Tested	Not recommended	Not recommended
Synthetic peptide - Human	Tested	Not recommended	Not recommended

Tested
Tested

Species	Dilution info	Notes
Species Recombinant fragment - Mouse, Synthetic peptide - Human	Dilution info -	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Human, Mouse	Dilution info Use at an assay dependent concentration.	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info -	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Mouse	Dilution info -	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species	Dilution info	Notes
Species Recombinant fragment - Mouse, Synthetic peptide - Human	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human, Mouse	Dilution info -	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Recombinant fragment - Mouse, Synthetic peptide - Human	Dilution info -	Notes -

Target data

See full target information MAPT

Function

Promotes microtubule assembly and stability, and might be involved in the establishment and maintenance of neuronal polarity (PubMed:21985311). The C-terminus binds axonal microtubules while the N-terminus binds neural plasma membrane components, suggesting that tau functions as a linker protein between both (PubMed:21985311, PubMed:32961270). Axonal polarity is predetermined by TAU/MAPT localization (in the neuronal cell) in the domain of the cell body defined by the centrosome. The short isoforms allow plasticity of the cytoskeleton whereas the longer isoforms may preferentially play a role in its stabilization.

Alternative names

MAPTL, MTBT1, TAU, MAPT, Microtubule-associated protein tau, Neurofibrillary tangle protein, Paired helical filament-tau, PHF-tau

Rabbit Recombinant Monoclonal TAU antibody. Carrier free. Suitable for I-ELISA, IHC-P, WB and reacts with Recombinant fragment - Mouse, Synthetic peptide - Human, Human, Mouse samples.

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Constituents: 100% PBS
Form: Liquid
Clonality: Monoclonal
Immunogen: The exact immunogen used to generate this antibody is proprietary information.
Carrier free: Yes
Clone number: EPR29542-63
Purification technique: Affinity purification Protein A
Specificity: The antibody binds to a region between the 4R domain and aa396 of 2N4R Tau.
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: +4°C

Notes

ab324110 is the carrier free version of Anti-Tau - C-terminal (4R-aa396) antibody [EPR29542-63] ab323709.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

10 product images

Western blot - Anti-Tau - C-terminal (4R-aa396) antibody [EPR29542-63] - BSA and Azide free (ab324110)
Tau - C-terminal (4R-aa396) Western blot staining using rabbit Anti-Tau - C-terminal (4R-aa396) antibody
This data was developed using Anti-Tau - C-terminal (4R-aa396) antibody [EPR29542-63] ab323709, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Negative control: liver spleen (PMID:24386422 PMID: 8752131)
The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 24116116 PMID: 8752131 PMID: 24386422 PMID:37443334).
The band around 15kDa in lane 1 might be cleavage fragment of Tau (PMID: 37905868).
In Western blot Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
Exposure time: Lane1: 125 seconds; Lanes 2-4: 6 seconds
All lanes: Western blot - Anti-Tau - C-terminal (4R-aa396) antibody [EPR29542-63] (Anti-Tau - C-terminal (4R-aa396) antibody [EPR29542-63] ab323709) at 1/1000 dilution
Lane 1: Human Alzheimers brain tissue lysate at 20 µg
Lane 2: Human hippocampus tissue lysate at 20 µg
Lane 3: Human spleen tissue lysate at 20 µg
Lane 4: Human liver tissue lysate at 20 µg
Secondary
All lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Observed band size: 33-62 kDa, 36 kDa
Western blot - Anti-Tau - C-terminal (4R-aa396) antibody [EPR29542-63] - BSA and Azide free (ab324110)
Tau - C-terminal (4R-aa396) Western blot staining using rabbit Anti-Tau - C-terminal (4R-aa396) antibody
This data was developed using Anti-Tau - C-terminal (4R-aa396) antibody [EPR29542-63] ab323709, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Negative control: liver spleen testis (PMID:24386422 PMID: 8752131)
The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 24116116 PMID: 8752131 PMID: 24386422 PMID:37443334).
The identity of the higher MW band at approximately 100 kDa in lane 7 is unknown.
In Western blot Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
All lanes: Western blot - Anti-Tau - C-terminal (4R-aa396) antibody [EPR29542-63] (Anti-Tau - C-terminal (4R-aa396) antibody [EPR29542-63] ab323709) at 1/1000 dilution
Lane 1: Mouse Alzheimers brain tissue lysate at 20 µg
Lane 2: Mouse brain tissue lysate at 20 µg
Lane 3: Mouse hippocampus tissue lysate at 20 µg
Lane 4: Mouse cerebellum tissue lysate at 20 µg
Lane 5: Mouse spleen tissue lysate at 20 µg
Lane 6: Mouse liver tissue lysate at 20 µg
Lane 7: Mouse testis tissue lysate at 20 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 33-62 kDa, 36 kDa
Exposure time: 37s
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau - C-terminal (4R-aa396) antibody [EPR29542-63] - BSA and Azide free (ab324110)
Tau - C-terminal (4R-aa396) Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining of Human cerebrum using rabbit Anti-Tau - C-terminal (4R-aa396) antibody
This data was developed using Anti-Tau - C-terminal (4R-aa396) antibody [EPR29542-63] ab323709, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labeling Tau - C-terminal (4R-aa396) with Anti-Tau - C-terminal (4R-aa396) antibody [EPR29542-63] ab323709 at 1/2000 (0.243 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on human cerebrum. The section was incubated with Anti-Tau - C-terminal (4R-aa396) antibody [EPR29542-63] ab323709 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0 Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau - C-terminal (4R-aa396) antibody [EPR29542-63] - BSA and Azide free (ab324110)
Tau - C-terminal (4R-aa396) Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining of Human Alzheimer's disease cerebrum using rabbit Anti-Tau - C-terminal (4R-aa396) antibody
This data was developed using Anti-Tau - C-terminal (4R-aa396) antibody [EPR29542-63] ab323709, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human Alzheimer's disease cerebrum tissue labeling Tau - C-terminal (4R-aa396) with Anti-Tau - C-terminal (4R-aa396) antibody [EPR29542-63] ab323709 at 1/2000 (0.243 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on human Alzheimer's disease cerebrum. The section was incubated with Anti-Tau - C-terminal (4R-aa396) antibody [EPR29542-63] ab323709 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0 Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau - C-terminal (4R-aa396) antibody [EPR29542-63] - BSA and Azide free (ab324110)
Tau - C-terminal (4R-aa396) Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining of Mouse cerebrum using rabbit Anti-Tau - C-terminal (4R-aa396) antibody
This data was developed using Anti-Tau - C-terminal (4R-aa396) antibody [EPR29542-63] ab323709, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling Tau - C-terminal (4R-aa396) with Anti-Tau - C-terminal (4R-aa396) antibody [EPR29542-63] ab323709 at 1/2000 (0.243 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on mouse cerebrum. The section was incubated with Anti-Tau - C-terminal (4R-aa396) antibody [EPR29542-63] ab323709 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0 Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau - C-terminal (4R-aa396) antibody [EPR29542-63] - BSA and Azide free (ab324110)
Tau - C-terminal (4R-aa396) Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining of Mouse Alzheimer's disease cerebrum using rabbit Anti-Tau - C-terminal (4R-aa396) antibody
This data was developed using Anti-Tau - C-terminal (4R-aa396) antibody [EPR29542-63] ab323709, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Mouse Alzheimer's disease cerebrum tissue labeling Tau - C-terminal (4R-aa396) with Anti-Tau - C-terminal (4R-aa396) antibody [EPR29542-63] ab323709 at 1/2000 (0.243 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on mouse Alzheimer's disease cerebrum. The section was incubated with Anti-Tau - C-terminal (4R-aa396) antibody [EPR29542-63] ab323709 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0 Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau - C-terminal (4R-aa396) antibody [EPR29542-63] - BSA and Azide free (ab324110)
Tau - C-terminal (4R-aa396) Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining of Human spleen using rabbit Anti-Tau - C-terminal (4R-aa396) antibody
This data was developed using Anti-Tau - C-terminal (4R-aa396) antibody [EPR29542-63] ab323709, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human spleen tissue labeling Tau - C-terminal (4R-aa396) with Anti-Tau - C-terminal (4R-aa396) antibody [EPR29542-63] ab323709 at 1/2000 (0.243 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Negative control: no staining on human spleen. The section was incubated with Anti-Tau - C-terminal (4R-aa396) antibody [EPR29542-63] ab323709 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0 Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau - C-terminal (4R-aa396) antibody [EPR29542-63] - BSA and Azide free (ab324110)
Tau - C-terminal (4R-aa396) Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining of Mouse spleen using rabbit Anti-Tau - C-terminal (4R-aa396) antibody
This data was developed using Anti-Tau - C-terminal (4R-aa396) antibody [EPR29542-63] ab323709, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue labeling Tau - C-terminal (4R-aa396) with Anti-Tau - C-terminal (4R-aa396) antibody [EPR29542-63] ab323709 at 1/2000 (0.243 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Negative control: no staining on mouse spleen (PMID: 24386422). The section was incubated with Anti-Tau - C-terminal (4R-aa396) antibody [EPR29542-63] ab323709 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0 Epitope Retrieval Solution2) for 20 mins
Indirect ELISA - Anti-Tau - C-terminal (4R-aa396) antibody [EPR29542-63] - BSA and Azide free (ab324110)
This data was developed using Anti-Tau - C-terminal (4R-aa396) antibody [EPR29542-63] ab323709, the same antibody clone in a different buffer formulation.
Indirect ELISA analysis of Anti-Tau - C-terminal (4R-aa396) antibody [EPR29542-63] ab323709 at 1000-0 ng/ml. The Secondary antibody used was Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1:2500 dilution dilution.
Antigen: Human Tau 2N4R peptide (within 4R-aa396), Human Tau 2N4R recombinant protein, Human MAP2 fragment.
Antigen concentration: 1000 ng/ml
This antibody does not cross-react with human MAP2.
Indirect ELISA - Anti-Tau - C-terminal (4R-aa396) antibody [EPR29542-63] - BSA and Azide free (ab324110)
This data was developed using Anti-Tau - C-terminal (4R-aa396) antibody [EPR29542-63] ab323709, the same antibody clone in a different buffer formulation.
Indirect ELISA analysis of Anti-Tau - C-terminal (4R-aa396) antibody [EPR29542-63] ab323709 at 1000-0 ng/ml. The Secondary antibody used was Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1:2500 dilution dilution.
Antigen: Human Tau 2N4R peptide (within 4R-aa396), Human Tau 2N4R recombinant protein, Human MAP2 fragment.
Antigen concentration: 1000 ng/ml
This antibody does not cross-react with human MAP2.