Anti-Tau - C-terminal antibody [EPR28785-33]

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau - C-terminal antibody [EPR28785-33] (AB316121)

Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labeling Tau - C-terminal with ab316121 at 1/4000 (0.122 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Cytoplasmic staining on human cerebrum (PMID : 2492045).
The section was incubated with ab316121 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau - C-terminal antibody [EPR28785-33] (AB316121)

Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling Tau - C-terminal with ab316121 at 1/4000 (0.122 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Cytoplasmic staining on peripheral nerves of human colon.
The section was incubated with ab316121 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau - C-terminal antibody [EPR28785-33] (AB316121)

Immunohistochemical analysis of paraffin-embedded Human spleen tissue labeling Tau - C-terminal with ab316121 at 1/4000 (0.122 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Negative control : No staining on human spleen (PMID : 12895417).
The section was incubated with ab316121 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau - C-terminal antibody [EPR28785-33] (AB316121)

Immunohistochemical analysis of paraffin-embedded Human Alzhiemer's disease brain tissue labeling Tau - C-terminal with ab316121 at 1/4000 (0.122 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond® Polymer Refine Detection).

Cytoplasmic staining on human Alzhiemer's disease brain.
The section was incubated with ab316121 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond® Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau antibody [EPR28785-33] (AB316121)

Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling Tau - C-terminal with ab316121 at 1/4000 (0.122 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Cytoplasmic staining on mouse cerebrum.
The section was incubated with ab316121 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-Tau - C-terminal antibody [EPR28785-33] (AB316121)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized mouse primary neuron cells labelling Tau - C-terminal with ab316121 at 1/50 (9.78 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2 ug/mL) dilution (Green).

Confocal image showing cytoplasmic staining in mouse primary neurons.
Confocal scanning Z step was set as 0.3 µm followed by image processing with maximum Z projection.
Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8).

ab11267 Anti-MAP2 mouse monoclonal antibody was used to counterstain at 1/500 (4ug/ml) dilution (Red). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/mL) dilution.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau antibody [EPR28785-33] (AB316121)

Immunohistochemical analysis of paraffin-embedded Rat cerebrum tissue labeling Tau - C-terminal with ab316121 at 1/4000 (0.122 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Cytoplasmic staining on rat cerebrum.
The section was incubated with ab316121 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau - C-terminal antibody [EPR28785-33] (AB316121)

Immunohistochemical analysis of paraffin-embedded Mouse colon tissue labeling Tau - C-terminal with ab316121 at 1/4000 (0.122 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Cytoplasmic staining on peripheral nerves of mouse colon.
The section was incubated with ab316121 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Tau - C-terminal antibody [EPR28785-33] (AB316121)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized rat primary neuron cells labelling Tau - C-terminal with ab316121 at 1/50 (9.78 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2 ug/mL) dilution (Green).

Confocal image showing cytoplasmic staining in rat primary neurons.
Confocal scanning Z step was set as 0.3 µm followed by image processing with maximum Z projection.
Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8).

ab11267 Anti-MAP2 mouse monoclonal antibody was used to counterstain at 1/500 (4ug/ml) dilution (Red). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/mL) dilution.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau - C-terminal antibody [EPR28785-33] (AB316121)

Immunohistochemical analysis of paraffin-embedded Rat colon tissue labeling Tau - C-terminal with ab316121 at 1/4000 (0.122 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Cytoplasmic staining on peripheral nerves of rat colon.
The section was incubated with ab316121 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• Flow Cyt (Intra)

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Flow Cytometry (Intracellular) - Anti-Tau - C-terminal antibody [EPR28785-33] (AB316121)

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized Mouse primary neuron cells labelling Tau - C-terminal with ab316121 at 1/50 dilution (1 ug)/Right compared with a Rabbit monoclonal IgG (ab172730) / Left isotype control.

Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.

• Flow Cyt (Intra)

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Flow Cytometry (Intracellular) - Anti-Tau - C-terminal antibody [EPR28785-33] (AB316121)

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized Rat primary neuron cells labelling Tau - C-terminal with ab316121 at 1/50 dilution (1 ug)/Right compared with a Rabbit monoclonal IgG (ab172730) / Left isotype control.

Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.

• WB

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Western blot - Anti-Tau - C-terminal antibody [EPR28785-33] (AB316121)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Negative control : spleen, kidney (PMID : 24386422; PMID : 24309898).

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-Tau - C-terminal antibody [EPR28785-33] (ab316121) at 1/1000 dilution

Lane 1:

Human brain tissue lysate at 20 µg

Lane 2:

Human kidney tissue lysate at 20 µg

Lane 3:

Human spleen tissue lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/1000 dilution

Observed band size: 33-70 kDa,36 kDa

false

Exposure time: 92s

• WB

Supplier Data

Western blot - Anti-Tau - C-terminal antibody [EPR28785-33] (AB316121)

All lanes:

Western blot - Anti-Tau (phospho T50) antibody [EPR29148-44] (<a href='/en-us/products/primary-antibodies/tau-phospho-t50-antibody-epr29148-44-ab322143'>ab322143</a>) at 1/1000 dilution

Lane 1:

Human brain tissue lysate (untreated membrane) at 20 µg

Lane 2:

Human brain tissue lysate (alkaline phosphatase treated membrane) at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Observed band size: 35-70 kDa,50-70 kDa

false

Exposure time: 92s

• WB

Supplier Data

Western blot - Anti-Tau - C-terminal antibody [EPR28785-33] (AB316121)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Negative control : heart, spleen (PMID : 24386422).

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-Tau - C-terminal antibody [EPR28785-33] (ab316121) at 1/1000 dilution

Lane 1:

Mouse hippocampus tissue lysate at 20 µg

Lane 2:

Mouse alzheimers brain tissue lysate at 20 µg

Lane 3:

Mouse brain tissue lysate at 20 µg

Lane 4:

Mouse heart tissue lysate at 20 µg

Lane 5:

Mouse spleen tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 45-70 kDa,36 kDa

false

Exposure time: 103s

• WB

Supplier Data

Western blot - Anti-Tau - C-terminal antibody [EPR28785-33] (AB316121)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Negative control : heart, spleen (PMID : 24386422).

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-Tau - C-terminal antibody [EPR28785-33] (ab316121) at 1/1000 dilution

Lane 1:

Rat hippocampus tissue lysate at 20 µg

Lane 2:

Rat brain tissue lysate at 20 µg

Lane 3:

Rat heart tissue lysate at 20 µg

Lane 4:

Rat spleen tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 45-70 kDa,36 kDa

false

Exposure time: 103s

• I-ELISA

Supplier Data

Indirect ELISA - Anti-Tau - C-terminal antibody [EPR28785-33] (AB316121)

Indirect ELISA analysis of ab316121 at 1000-0 ng/ml. The Secondary antibody used was Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1 : 2500 dilution.

Antigen : Human 1N3R, Human 1N4R, Human 2N4R.

Antigen concentration : 1000 ng/ml

• WB

Supplier Data

Western blot - Anti-Tau - C-terminal antibody [EPR28785-33] (AB316121)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

The identity of the bands higher than 100 kDa are unknown.

In Western blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (ab213204) staining at 1/5000 dilution.

In Western blot, Anti-Tau - C-terminal antibody [EPR28785-33] - (ab316121) staining at 1/1000 dilution.

Actual observed bands 50-70kDa.

All lanes:

Western blot - Anti-Tau (phospho T50) antibody [EPR29148-44] (<a href='/en-us/products/primary-antibodies/tau-phospho-t50-antibody-epr29148-44-ab322143'>ab322143</a>) at 1/1000 dilution

Lane 1:

293T (human embryonic kidney epithelial cell) cells transfected with a human Tau (T50A mutation) expression vector containing a myc-His-tag® treated with 100nM Calyculin A for 30min, whole cell lysate (untreated membrane) at 20 µg

Lane 2:

293T cells transfected with a human wild-type Tau expression vector containing a myc-His-tag® treated with 100nM Calyculin A for 30min, whole cell lysate (untreated membrane) at 20 µg

Lane 3:

293T cells transfected with a human wild-type Tau expression vector containing a myc-His-tag® treated with 100nM Calyculin A for 30min, whole cell lysate (alkaline phosphatase treated membrane) at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 50-70 kDa

false

Exposure time: 8s