Skip to main content

Rabbit Recombinant Monoclonal TAU antibody. Suitable for WB, IHC-P, ICC/IF, I-ELISA, Flow Cyt (Intra) and reacts with Human, Mouse, Rat, Recombinant fragment - Human samples.

Be the first to review this product! Submit a review

Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau - C-terminal antibody [EPR28785-33] (AB316121), expandable thumbnail
  • Western blot - Anti-Tau - C-terminal antibody [EPR28785-33] (AB316121), expandable thumbnail
  • Western blot - Anti-Tau - C-terminal antibody [EPR28785-33] (AB316121), expandable thumbnail
  • Indirect ELISA - Anti-Tau - C-terminal antibody [EPR28785-33] (AB316121), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-Tau - C-terminal antibody [EPR28785-33] (AB316121), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIHC-PICC/IFI-ELISAFlow Cyt (Intra)
Human
Tested
Tested
Expected
Expected
Expected
Mouse
Tested
Tested
Tested
Expected
Tested
Rat
Tested
Tested
Tested
Expected
Tested
Recombinant fragment - Human
Not recommended
Not recommended
Not recommended
Tested
Not recommended

Tested
Tested

Species

Human

Dilution info
1/1000
Notes

-

Species

Mouse

Dilution info
1/1000
Notes

-

Species

Rat

Dilution info
1/1000
Notes

-

Not recommended
Not recommended

Species

Recombinant fragment - Human

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info
1/4000
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species

Mouse

Dilution info
1/4000
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species

Rat

Dilution info
1/4000
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species

Recombinant fragment - Human

Dilution info

-

Notes

-

Tested
Tested

Species

Mouse

Dilution info
1/50
Notes

-

Species

Rat

Dilution info
1/50
Notes

-

Expected
Expected

Species

Human

Dilution info

Use at an assay dependent concentration.

Notes

-

Not recommended
Not recommended

Species

Recombinant fragment - Human

Dilution info

-

Notes

-

Tested
Tested

Species

Recombinant fragment - Human

Dilution info
1000 ng/mL
Notes

-

Expected
Expected

Species

Human, Mouse, Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Mouse

Dilution info
1/50
Notes

-

Species

Rat

Dilution info
1/50
Notes

-

Expected
Expected

Species

Human

Dilution info

Use at an assay dependent concentration.

Notes

-

Not recommended
Not recommended

Species

Recombinant fragment - Human

Dilution info

-

Notes

-

Associated Products

Select an associated product type

1 product for Alternative Version

Target data

Function

Promotes microtubule assembly and stability, and might be involved in the establishment and maintenance of neuronal polarity (PubMed:21985311). The C-terminus binds axonal microtubules while the N-terminus binds neural plasma membrane components, suggesting that tau functions as a linker protein between both (PubMed:21985311, PubMed:32961270). Axonal polarity is predetermined by TAU/MAPT localization (in the neuronal cell) in the domain of the cell body defined by the centrosome. The short isoforms allow plasticity of the cytoskeleton whereas the longer isoforms may preferentially play a role in its stabilization.

Alternative names

Recommended products

Rabbit Recombinant Monoclonal TAU antibody. Suitable for WB, IHC-P, ICC/IF, I-ELISA, Flow Cyt (Intra) and reacts with Human, Mouse, Rat, Recombinant fragment - Human samples.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EPR28785-33

Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Tau also known as microtubule-associated protein Tau (MAPT) plays an important role in stabilizing microtubules in neuronal cells. Tau is primarily found in the central nervous system but also exists in peripheral neurons. Human Tau protein comes in six isoforms due to alternative splicing with molecular weights ranging from 48 kDa to 67 kDa. This protein predominantly locates in the axons of neurons where it maintains the stability of microtubule tracks necessary for axonal transport.

Biological function summary

Tau is involved in the assembly and stabilization of microtubules essential for maintaining neuronal structure. It interacts with microtubule-binding domains (MBD) to bind and bundle microtubules facilitating intracellular transport. Tau forms a part of the neuronal cytoskeleton complex working closely with other cytoskeletal proteins to preserve the proper axonal transport and function. Abnormally phosphorylated Tau often termed phospho-Tau disrupts this complex affecting microtubule stability.

Pathways

Tau has critical involvement in several signaling cascades such as the microtubule-binding and transport pathways. Glycogen synthase kinase 3 beta (GSK3β) and cyclin-dependent kinase 5 (CDK5) frequently phosphorylate Tau controlling its interaction with microtubules. Phosphorylated Tau accumulates leading to the formation of neurofibrillary tangles often observed in neurodegenerative conditions. Additionally Tau interacts with GAPDH impacting cellular energy regulation through potential pathway cross-talk involving oxidative stress responses.

Associated diseases and disorders

Tau is closely associated with Alzheimer's disease and frontotemporal dementia. In Alzheimer's disease hyperphosphorylated Tau aggregates into paired helical filaments forming neurofibrillary tangles while similar aggregates are observed in frontotemporal dementia. In these conditions Tau links to amyloid precursor protein (APP) where misregulated phosphorylation-driven interactions contribute to neurodegeneration. Identifying phospho-Tau and its altered interactions with related proteins aids in understanding and potentially treating these disorders.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

18 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau - C-terminal antibody [EPR28785-33] (ab316121), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau - C-terminal antibody [EPR28785-33] (ab316121)

    Immunohistochemical analysis of paraffin-embedded Human Alzhiemer's disease brain tissue labeling Tau - C-terminal with ab316121 at 1/4000 (0.122 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond® Polymer Refine Detection).

    Cytoplasmic staining on human Alzhiemer's disease brain.

    The section was incubated with ab316121 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond® Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

  • Western blot - Anti-Tau - C-terminal antibody [EPR28785-33] (ab316121), expandable thumbnail

    Western blot - Anti-Tau - C-terminal antibody [EPR28785-33] (ab316121)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    The identity of the bands higher than 100 kDa are unknown.

    In Western blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (Anti-6X His tag® antibody [EPR20547] - ChIP Grade ab213204) staining at 1/5000 dilution.

    In Western blot, Anti-Tau - C-terminal antibody [EPR28785-33] - (ab316121) staining at 1/1000 dilution.

    Actual observed bands 50-70kDa.

    All lanes: Western blot - Anti-Tau (phospho T50) antibody [EPR29148-44] (Anti-Tau (phospho T50) antibody [EPR29148-44] ab322143) at 1/1000 dilution

    Lane 1: 293T (human embryonic kidney epithelial cell) cells transfected with a human Tau (T50A mutation) expression vector containing a myc-His-tag® treated with 100nM Calyculin A for 30min, whole cell lysate (untreated membrane) at 20 µg

    Lane 2: 293T cells transfected with a human wild-type Tau expression vector containing a myc-His-tag® treated with 100nM Calyculin A for 30min, whole cell lysate (untreated membrane) at 20 µg

    Lane 3: 293T cells transfected with a human wild-type Tau expression vector containing a myc-His-tag® treated with 100nM Calyculin A for 30min, whole cell lysate (alkaline phosphatase treated membrane) at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Observed band size: 50-70 kDa

    Exposure time: 8s

  • Western blot - Anti-Tau - C-terminal antibody [EPR28785-33] (ab316121), expandable thumbnail

    Western blot - Anti-Tau - C-terminal antibody [EPR28785-33] (ab316121)

    All lanes: Western blot - Anti-Tau (phospho T50) antibody [EPR29148-44] (Anti-Tau (phospho T50) antibody [EPR29148-44] ab322143) at 1/1000 dilution

    Lane 1: Human brain tissue lysate (untreated membrane) at 20 µg

    Lane 2: Human brain tissue lysate (alkaline phosphatase treated membrane) at 20 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Observed band size: 35-70 kDa, 50-70 kDa

    Exposure time: 92s

  • Indirect ELISA - Anti-Tau - C-terminal antibody [EPR28785-33] (ab316121), expandable thumbnail

    Indirect ELISA - Anti-Tau - C-terminal antibody [EPR28785-33] (ab316121)

    Indirect ELISA analysis of ab316121 at 1000-0 ng/ml. The Secondary antibody used was Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1:2500 dilution.

    Antigen: Human 1N3R, Human 1N4R, Human 2N4R.

    Antigen concentration: 1000 ng/ml

  • Flow Cytometry (Intracellular) - Anti-Tau - C-terminal antibody [EPR28785-33] (ab316121), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-Tau - C-terminal antibody [EPR28785-33] (ab316121)

    Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized Rat primary neuron cells labelling Tau - C-terminal with ab316121 at 1/50 dilution (1 ug)/Right compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) / Left isotype control.

    Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/5000 dilution was used as the secondary antibody.

  • Flow Cytometry (Intracellular) - Anti-Tau - C-terminal antibody [EPR28785-33] (ab316121), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-Tau - C-terminal antibody [EPR28785-33] (ab316121)

    Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized Mouse primary neuron cells labelling Tau - C-terminal with ab316121 at 1/50 dilution (1 ug)/Right compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) / Left isotype control.

    Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/5000 dilution was used as the secondary antibody.

  • Immunocytochemistry/ Immunofluorescence - Anti-Tau - C-terminal antibody [EPR28785-33] (ab316121), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Tau - C-terminal antibody [EPR28785-33] (ab316121)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized rat primary neuron cells labelling Tau - C-terminal with ab316121 at 1/50 (9.78 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2 ug/mL) dilution (Green).

    Confocal image showing cytoplasmic staining in rat primary neurons.

    Confocal scanning Z step was set as 0.3 ?m followed by image processing with maximum Z projection.

    Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8).

    Anti-MAP2 antibody [HM-2] ab11267 Anti-MAP2 mouse monoclonal antibody was used to counterstain at 1/500 (4ug/ml) dilution (Red). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/mL) dilution.

  • Immunocytochemistry/ Immunofluorescence - Anti-Tau - C-terminal antibody [EPR28785-33] (ab316121), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Tau - C-terminal antibody [EPR28785-33] (ab316121)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized mouse primary neuron cells labelling Tau - C-terminal with ab316121 at 1/50 (9.78 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2 ug/mL) dilution (Green).

    Confocal image showing cytoplasmic staining in mouse primary neurons.

    Confocal scanning Z step was set as 0.3 ?m followed by image processing with maximum Z projection.

    Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8).

    Anti-MAP2 antibody [HM-2] ab11267 Anti-MAP2 mouse monoclonal antibody was used to counterstain at 1/500 (4ug/ml) dilution (Red). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/mL) dilution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau - C-terminal antibody [EPR28785-33] (ab316121), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau - C-terminal antibody [EPR28785-33] (ab316121)

    Immunohistochemical analysis of paraffin-embedded Human spleen tissue labeling Tau - C-terminal with ab316121 at 1/4000 (0.122 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Negative control: No staining on human spleen (PMID:12895417).

    The section was incubated with ab316121 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau - C-terminal antibody [EPR28785-33] (ab316121), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau - C-terminal antibody [EPR28785-33] (ab316121)

    Immunohistochemical analysis of paraffin-embedded Rat colon tissue labeling Tau - C-terminal with ab316121 at 1/4000 (0.122 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Cytoplasmic staining on peripheral nerves of rat colon.

    The section was incubated with ab316121 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau - C-terminal antibody [EPR28785-33] (ab316121), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau - C-terminal antibody [EPR28785-33] (ab316121)

    Immunohistochemical analysis of paraffin-embedded Mouse colon tissue labeling Tau - C-terminal with ab316121 at 1/4000 (0.122 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Cytoplasmic staining on peripheral nerves of mouse colon.

    The section was incubated with ab316121 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau - C-terminal antibody [EPR28785-33] (ab316121), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau - C-terminal antibody [EPR28785-33] (ab316121)

    Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling Tau - C-terminal with ab316121 at 1/4000 (0.122 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Cytoplasmic staining on peripheral nerves of human colon.

    The section was incubated with ab316121 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau - C-terminal antibody [EPR28785-33] (ab316121), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau - C-terminal antibody [EPR28785-33] (ab316121)

    Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labeling Tau - C-terminal with ab316121 at 1/4000 (0.122 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Cytoplasmic staining on human cerebrum (PMID: 2492045).

    The section was incubated with ab316121 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

  • Western blot - Anti-Tau - C-terminal antibody [EPR28785-33] (ab316121), expandable thumbnail

    Western blot - Anti-Tau - C-terminal antibody [EPR28785-33] (ab316121)

    Negative control: heart, spleen (PMID: 24386422).

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    All lanes: Western blot - Anti-Tau - C-terminal antibody [EPR28785-33] (ab316121) at 1/1000 dilution

    Lane 1: Mouse hippocampus tissue lysate at 20 µg

    Lane 2: Mouse alzheimers brain tissue lysate at 20 µg

    Lane 3: Mouse brain tissue lysate at 20 µg

    Lane 4: Mouse heart tissue lysate at 20 µg

    Lane 5: Mouse spleen tissue lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Observed band size: 45-70 kDa, 36 kDa

    Exposure time: 103s

  • Western blot - Anti-Tau antibody [EPR28785-33] (ab316121), expandable thumbnail

    Western blot - Anti-Tau antibody [EPR28785-33] (ab316121)

    Negative control: heart, spleen (PMID: 24386422).

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    All lanes: Western blot - Anti-Tau antibody [EPR28785-33] (ab316121) at 1/1000 dilution

    Lane 1: Rat hippocampus tissue lysate at 20 µg

    Lane 2: Rat brain tissue lysate at 20 µg

    Lane 3: Rat heart tissue lysate at 20 µg

    Lane 4: Rat spleen tissue lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Observed band size: 45-70 kDa, 36 kDa

    Exposure time: 103s

  • Western blot - Anti-Tau antibody [EPR28785-33] (ab316121), expandable thumbnail

    Western blot - Anti-Tau antibody [EPR28785-33] (ab316121)

    Negative control: spleen, kidney (PMID: 24386422; PMID: 24309898).

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    All lanes: Western blot - Anti-Tau antibody [EPR28785-33] (ab316121) at 1/1000 dilution

    Lane 1: Human brain tissue lysate at 20 µg

    Lane 2: Human kidney tissue lysate at 20 µg

    Lane 3: Human spleen tissue lysate at 20 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/1000 dilution

    Observed band size: 33-70 kDa, 36 kDa

    Exposure time: 92s

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau antibody [EPR28785-33] (ab316121), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau antibody [EPR28785-33] (ab316121)

    Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling Tau - C-terminal with ab316121 at 1/4000 (0.122 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Cytoplasmic staining on mouse cerebrum.

    The section was incubated with ab316121 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau antibody [EPR28785-33] (ab316121), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau antibody [EPR28785-33] (ab316121)

    Immunohistochemical analysis of paraffin-embedded Rat cerebrum tissue labeling Tau - C-terminal with ab316121 at 1/4000 (0.122 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Cytoplasmic staining on rat cerebrum.

    The section was incubated with ab316121 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com