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Rabbit Recombinant Monoclonal TAU antibody. Suitable for WB, IHC-P, IHC-Fr, ICC/IF, Flow Cyt (Intra), IP and reacts with Transfected cell lysate - Human, Human, Mouse, Rat samples.

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Images

Western blot - Anti-Tau (MBD region) antibody [EPR25205-233] (AB308439), expandable thumbnail
  • Affinity Purification - Anti-Tau (MBD region) antibody [EPR25205-233] (AB308439), expandable thumbnail
  • Immunohistochemistry (Frozen sections) - Anti-Tau (MBD region) antibody [EPR25205-233] (AB308439), expandable thumbnail
  • Immunohistochemistry (Frozen sections) - Anti-Tau (MBD region) antibody [EPR25205-233] (AB308439), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-Tau (MBD region) antibody [EPR25205-233] (AB308439), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIHC-PIHC-FrICC/IFFlow Cyt (Intra)IP
Human
Tested
Tested
Expected
Expected
Expected
Expected
Mouse
Tested
Tested
Tested
Tested
Tested
Tested
Rat
Tested
Tested
Tested
Tested
Tested
Expected
Transfected cell lysate - Human
Tested
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended

Tested
Tested

Species

Transfected cell lysate - Human

Dilution info
1/1000
Notes

-

Species

Human

Dilution info
1/1000
Notes

-

Species

Mouse

Dilution info
1/1000
Notes

-

Species

Rat

Dilution info
1/1000
Notes

-

Tested
Tested

Species

Human

Dilution info
1/1000
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species

Mouse

Dilution info
1/1000
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species

Rat

Dilution info
1/1000
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species

Transfected cell lysate - Human

Dilution info

-

Notes

-

Tested
Tested

Species

Mouse

Dilution info
1/50
Notes

-

Species

Rat

Dilution info
1/50
Notes

-

Expected
Expected

Species

Human

Dilution info

Use at an assay dependent concentration.

Notes

-

Not recommended
Not recommended

Species

Transfected cell lysate - Human

Dilution info

-

Notes

-

Tested
Tested

Species

Mouse

Dilution info
1/50
Notes

-

Species

Rat

Dilution info
1/50
Notes

-

Expected
Expected

Species

Human

Dilution info

Use at an assay dependent concentration.

Notes

-

Not recommended
Not recommended

Species

Transfected cell lysate - Human

Dilution info

-

Notes

-

Tested
Tested

Species

Mouse

Dilution info
1/500
Notes

-

Species

Rat

Dilution info
1/500
Notes

-

Expected
Expected

Species

Human

Dilution info

Use at an assay dependent concentration.

Notes

-

Not recommended
Not recommended

Species

Transfected cell lysate - Human

Dilution info

-

Notes

-

Tested
Tested

Species

Mouse

Dilution info
1/30
Notes

-

Expected
Expected

Species

Human, Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Not recommended
Not recommended

Species

Transfected cell lysate - Human

Dilution info

-

Notes

-

Target data

Function

Promotes microtubule assembly and stability, and might be involved in the establishment and maintenance of neuronal polarity (PubMed:21985311). The C-terminus binds axonal microtubules while the N-terminus binds neural plasma membrane components, suggesting that tau functions as a linker protein between both (PubMed:21985311, PubMed:32961270). Axonal polarity is predetermined by TAU/MAPT localization (in the neuronal cell) in the domain of the cell body defined by the centrosome. The short isoforms allow plasticity of the cytoskeleton whereas the longer isoforms may preferentially play a role in its stabilization.

Alternative names

Recommended products

Rabbit Recombinant Monoclonal TAU antibody. Suitable for WB, IHC-P, IHC-Fr, ICC/IF, Flow Cyt (Intra), IP and reacts with Transfected cell lysate - Human, Human, Mouse, Rat samples.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EPR25205-233

Purification technique

Affinity purification Protein A

Dissociation constant
4.78 x 10-10 M
Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Tau also known as microtubule-associated protein Tau (MAPT) plays an important role in stabilizing microtubules in neuronal cells. Tau is primarily found in the central nervous system but also exists in peripheral neurons. Human Tau protein comes in six isoforms due to alternative splicing with molecular weights ranging from 48 kDa to 67 kDa. This protein predominantly locates in the axons of neurons where it maintains the stability of microtubule tracks necessary for axonal transport.

Biological function summary

Tau is involved in the assembly and stabilization of microtubules essential for maintaining neuronal structure. It interacts with microtubule-binding domains (MBD) to bind and bundle microtubules facilitating intracellular transport. Tau forms a part of the neuronal cytoskeleton complex working closely with other cytoskeletal proteins to preserve the proper axonal transport and function. Abnormally phosphorylated Tau often termed phospho-Tau disrupts this complex affecting microtubule stability.

Pathways

Tau has critical involvement in several signaling cascades such as the microtubule-binding and transport pathways. Glycogen synthase kinase 3 beta (GSK3β) and cyclin-dependent kinase 5 (CDK5) frequently phosphorylate Tau controlling its interaction with microtubules. Phosphorylated Tau accumulates leading to the formation of neurofibrillary tangles often observed in neurodegenerative conditions. Additionally Tau interacts with GAPDH impacting cellular energy regulation through potential pathway cross-talk involving oxidative stress responses.

Associated diseases and disorders

Tau is closely associated with Alzheimer's disease and frontotemporal dementia. In Alzheimer's disease hyperphosphorylated Tau aggregates into paired helical filaments forming neurofibrillary tangles while similar aggregates are observed in frontotemporal dementia. In these conditions Tau links to amyloid precursor protein (APP) where misregulated phosphorylation-driven interactions contribute to neurodegeneration. Identifying phospho-Tau and its altered interactions with related proteins aids in understanding and potentially treating these disorders.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

19 product images

  • Western blot - Anti-Tau (MBD region) antibody [EPR25205-233] (ab308439), expandable thumbnail

    Western blot - Anti-Tau (MBD region) antibody [EPR25205-233] (ab308439)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Negative control: spleen (PMID: 24309898; PMID:24386422).

    In Western blot, anti- Vinculin antibody (Anti-Vinculin antibody [EPR8185] ab129002) loading control staining at 1/10, 000 dilution.

    This blot was developed using a high sensitivity ECL substrate. The high-sensitivity ECL substrate used allows for the detection of proteins in the mid-femtogram range.

    All lanes: Western blot - Anti-Tau (MBD region) antibody [EPR25205-233] (ab308439) at 1/1000 dilution

    Lane 1: Mouse brain tissue lysate at 20 µg

    Lane 2: Mouse spleen tissue lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Developed using the ECL technique.

    Predicted band size: 78 kDa

    Observed band size: 50 kDa

    Exposure time: 180s

  • Affinity Purification - Anti-Tau (MBD region) antibody [EPR25205-233] (ab308439), expandable thumbnail

    Affinity Purification - Anti-Tau (MBD region) antibody [EPR25205-233] (ab308439)

    Biotinylated Human Tau 2N4R (Protein: P9954) [1μg/ml] is loaded to SA biosensor on Octet RED96e machine, then associate with Anti-Tau (MBD region) antibody [EPR25205-233] (Anti-Tau (MBD region) antibody [EPR25205-233] - BSA and Azide free ab308440) in serial concentration points [53.35, 26.68, 13.34, 6.67, 3.34 nM/ml] by 2-fold dilution, then dissociate in blank testing buffer [10mM HEPES (0.05% Tween-20, 3mM EDTA, 100mM NaCl)]. Calculated signals had already subtracted blank control, curve fitting using 1:1 binding model. KD (M) value of Anti-Tau (MBD region) antibody [EPR25205-233] (Anti-Tau (MBD region) antibody [EPR25205-233] - BSA and Azide free ab308440) is 4.78E-10.

  • Immunohistochemistry (Frozen sections) - Anti-Tau (MBD region) antibody [EPR25205-233] (ab308439), expandable thumbnail

    Immunohistochemistry (Frozen sections) - Anti-Tau (MBD region) antibody [EPR25205-233] (ab308439)

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse spleen (fresh) tissue labeling Tau (MBD region) with ab308439 at 1/50 (9.64 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution (Green). Negative control: confocal image showing no staining on mouse spleen (PMID: 24309898). The nuclear counterstain was DAPI (Blue). The section was incubated with ab308439 for 60 mins at room temperature. The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). is observed. The nuclear counterstain was DAPI (Blue).

    Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution.

  • Immunohistochemistry (Frozen sections) - Anti-Tau (MBD region) antibody [EPR25205-233] (ab308439), expandable thumbnail

    Immunohistochemistry (Frozen sections) - Anti-Tau (MBD region) antibody [EPR25205-233] (ab308439)

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse cerebellum (fresh) tissue labeling Tau (MBD region) with ab308439 at 1/50 (9.64 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution (Green). Confocal image showing positive staining on mouse cerebellum. The nuclear counterstain was DAPI (Blue). The section was incubated with ab308439 for 60 mins at room temperature. The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). is observed. The nuclear counterstain was DAPI (Blue).

    Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution.

  • Immunocytochemistry/ Immunofluorescence - Anti-Tau (MBD region) antibody [EPR25205-233] (ab308439), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Tau (MBD region) antibody [EPR25205-233] (ab308439)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized rat primary neuron cells labelling Tau (MBD region) with ab308439 at 1/50 (9.64 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2ug/mL dilution (Green). Confocal image showing cytoplasmic staining in rat primary neuron.Confocal scanning Z step was set as 0.3 ?m followed by image processing with maximum Z projection Anti-MAP2 antibody [HM-2] ab11267 Anti-MAP2 mouse monoclonal antibody was used to counterstain tubulin at 1/500 4ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2ug/mL dilution.

  • Immunocytochemistry/ Immunofluorescence - Anti-Tau (MBD region) antibody [EPR25205-233] (ab308439), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Tau (MBD region) antibody [EPR25205-233] (ab308439)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized mouse primary neuron cells labelling Tau (MBD region) with ab308439 at 1/50 (9.64 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2ug/mL dilution (Green). Confocal image showing cytoplasmic staining in mouse primary neuron.Confocal scanning Z step was set as 0.3 ?m followed by image processing with maximum Z projection Anti-MAP2 antibody [HM-2] ab11267 Anti-MAP2 mouse monoclonal antibody was used to counterstain tubulin at 1/500 4ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2ug/mL dilution.

  • Flow Cytometry (Intracellular) - Anti-Tau (MBD region) antibody [EPR25205-233] (ab308439), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-Tau (MBD region) antibody [EPR25205-233] (ab308439)

    Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized rat primary neuron cells labelling Tau (MBD region) with ab308439 at 1/500 dilution (0.1 ug)/Right (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/5000 dilution was used as the secondary antibody.

  • Flow Cytometry (Intracellular) - Anti-Tau (MBD region) antibody [EPR25205-233] (ab308439), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-Tau (MBD region) antibody [EPR25205-233] (ab308439)

    Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized mouse primary neuron cells labelling Tau (MBD region) with ab308439 at 1/500 dilution (0.1 ug)/Right (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/5000 dilution was used as the secondary antibody.

  • Western blot - Anti-Tau (MBD region) antibody [EPR25205-233] (ab308439), expandable thumbnail

    Western blot - Anti-Tau (MBD region) antibody [EPR25205-233] (ab308439)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Negative control: testis (PMID: 24386422).

    In Western blot, anti-GAPDH antibody (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) loading control staining at 1/200, 000 dilution.

    Exposure time: 180 seconds.

    All lanes: Western blot - Anti-Tau (MBD region) antibody [EPR25205-233] (ab308439) at 1/1000 dilution

    Lane 1: Rat brain tissue lysate at 20 µg

    Lane 2: Rat testis tissue lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Predicted band size: 78 kDa

    Observed band size: 50-70 kDa

    Exposure time: 180s

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (MBD region) antibody [EPR25205-233] (ab308439), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (MBD region) antibody [EPR25205-233] (ab308439)

    Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue labelling Tau (MBD region) with ab308439 at 1/5000 (0.096 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Negative control: no staining in mouse spleen. The section was incubated with ab308439 for 30 mins at room temperature. The
    immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

  • Immunoprecipitation - Anti-Tau (MBD region) antibody [EPR25205-233] (ab308439), expandable thumbnail

    Immunoprecipitation - Anti-Tau (MBD region) antibody [EPR25205-233] (ab308439)

    Tau (MBD region) was immunoprecipitated from 0.35 mg Mouse brain tissue lysate with ab308439 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab308439 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.

    Lane 1: Mouse brain tissue lysate

    Lane 2: abAB308439 IP in Mouse brain tissue lysate

    Lane 3:Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab308439 in mouse brain tissue lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 180 seconds

    All lanes: Immunoprecipitation - Anti-Tau (MBD region) antibody [EPR25205-233] (ab308439) at 1/30 dilution

    All lanes: Mouse brain tissue lysate at 10 µg

    Secondary

    All lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution

    Observed band size: 33 kDa, 50 kDa

    Exposure time: 180s

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (MBD region) antibody [EPR25205-233] (ab308439), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (MBD region) antibody [EPR25205-233] (ab308439)

    Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labelling Tau (MBD region) with ab308439 at 1/5000 (0.096 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Positive staining in mouse cerebrum. The section was incubated with ab308439 for 30 mins at room temperature. The
    immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

  • Western blot - Anti-Tau (MBD region) antibody [EPR25205-233] (ab308439), expandable thumbnail

    Western blot - Anti-Tau (MBD region) antibody [EPR25205-233] (ab308439)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    In Western blot, anti-His antibody (Anti-6X His tag® antibody [EPR20547] - ChIP Grade ab213204) staining at 1/5000 dilution.

    Exposure time: 8 seconds.

    All lanes: Western blot - Anti-Tau (MBD region) antibody [EPR25205-233] (ab308439) at 1/1000 dilution

    Lane 1: HEK-293 transfected with Tau 0N3R (WT) expression vector containing a myc-His-tag®, whole cell lysate at 20 µg

    Lane 2: HEK-293 transfected with Tau 1N3R (WT) expression vector containing a myc-His-tag®, whole cell lysate at 20 µg

    Lane 3: HEK-293 transfected with Tau 2N3R (WT) expression vector containing a myc-His-tag®, whole cell lysate at 20 µg

    Lane 4: HEK-293 transfected with Tau 0N4R (WT) expression vector containing a myc-His-tag®, whole cell lysate at 20 µg

    Lane 5: HEK-293 transfected with Tau 1N4R (WT) expression vector containing a myc-His-tag®, whole cell lysate at 20 µg

    Lane 6: HEK-293 transfected with Tau 2N4R (WT) expression vector containing a myc-His-tag®, whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 78 kDa

    Observed band size: 10-70 kDa

    Exposure time: 8s

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (MBD region) antibody [EPR25205-233] (ab308439), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (MBD region) antibody [EPR25205-233] (ab308439)

    Immunohistochemical analysis of paraffin-embedded Rat cerebrum tissue labelling Tau (MBD region) with ab308439 at 1/5000 (0.096 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Positive staining in rat cerebrum. The section was incubated with ab308439 for 30 mins at room temperature. The
    immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

  • Western blot - Anti-Tau (MBD region) antibody [EPR25205-233] (ab308439), expandable thumbnail

    Western blot - Anti-Tau (MBD region) antibody [EPR25205-233] (ab308439)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Negative control: spleen (PMID: 24309898; PMID:24386422), kidney (PMID: 24386422).

    In Western blot, anti-GAPDH antibody (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) loading control staining at 1/200,000 dilution.

    Exposure times: Lane 1:26 seconds; Lanes 2-4: 81 seconds.

    All lanes: Western blot - Anti-Tau (MBD region) antibody [EPR25205-233] (ab308439) at 1/1000 dilution

    Lane 1: Human brain tissue lysate at 20 µg

    Lane 2: Human cerebellum tissue lysate at 20 µg

    Lane 3: Human kidney tissue lysate at 20 µg

    Lane 4: Human spleen tissue lysate at 20 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Predicted band size: 78 kDa

    Observed band size: 33-50 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (MBD region) antibody [EPR25205-233] (ab308439), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (MBD region) antibody [EPR25205-233] (ab308439)

    Immunohistochemical analysis of paraffin-embedded Human spleen tissue labelling Tau (MBD region) with ab308439 at 1/1000 (0.482 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Negative control: no staining in human spleen. The section was incubated with ab308439 for 30 mins at room temperature. The
    immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (MBD region) antibody [EPR25205-233] (ab308439), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (MBD region) antibody [EPR25205-233] (ab308439)

    Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labelling Tau (MBD region) with ab308439 at 1/1000 (0.482 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Positive staining in human cerebrum. The section was incubated with ab308439 for 30 mins at room temperature. The
    immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

  • Immunohistochemistry (Frozen sections) - Anti-Tau (MBD region) antibody [EPR25205-233] (ab308439), expandable thumbnail

    Immunohistochemistry (Frozen sections) - Anti-Tau (MBD region) antibody [EPR25205-233] (ab308439)

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat spleen (fresh) tissue labeling Tau (MBD region) with ab308439 at 1/50 (9.64 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution (Green). Negative control: confocal image showing no staining on rat spleen (PMID: 24309898). The nuclear counterstain was DAPI (Blue). The section was incubated with ab308439 for 60 mins at room temperature. The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). is observed. The nuclear counterstain was DAPI (Blue).

    Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbedat 1/1000 2 ug/mL dilution.

  • Immunohistochemistry (Frozen sections) - Anti-Tau (MBD region) antibody [EPR25205-233] (ab308439), expandable thumbnail

    Immunohistochemistry (Frozen sections) - Anti-Tau (MBD region) antibody [EPR25205-233] (ab308439)

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat cerebellum (fresh) tissue labeling Tau (MBD region) with ab308439 at 1/50 (9.64 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution (Green). Confocal image showing positive staining on rat cerebellum. The nuclear counterstain was DAPI (Blue). The section was incubated with ab308439 for 60 mins at room temperature. The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). is observed. The nuclear counterstain was DAPI (Blue).

    Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution.

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