Anti-Tau (MTBR-tau243) antibody [EPR29541-59] (ab322554)

Rabbit Recombinant Monoclonal TAU antibody. Suitable for WB, I-ELISA, IHC-P and reacts with Transfected cell lysate - Human, Human, Mouse, Recombinant fragment - Human, Synthetic peptide - Human, Recombinant fragment - Mouse samples.

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Images

Indirect ELISA - Anti-Tau (MTBR-tau243) antibody [EPR29541-59] (AB322554), expandable thumbnail

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	WB	I-ELISA	IHC-P
Human	Tested	Expected	Tested
Mouse	Tested	Expected	Tested
Recombinant fragment - Human	Not recommended	Tested	Not recommended
Recombinant fragment - Mouse	Not recommended	Tested	Not recommended
Synthetic peptide - Human	Not recommended	Tested	Not recommended
Transfected cell lysate - Human	Tested	Not recommended	Not recommended

Tested
Tested

Species	Dilution info	Notes
Species Transfected cell lysate - Human	Dilution info 1/1000	Notes -
Species Human	Dilution info 1/1000	Notes -
Species Mouse	Dilution info 1/1000	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Recombinant fragment - Human, Synthetic peptide - Human, Recombinant fragment - Mouse	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Recombinant fragment - Human	Dilution info 1000 ng/mL	Notes -
Species Synthetic peptide - Human	Dilution info 1000 ng/mL	Notes -
Species Recombinant fragment - Mouse	Dilution info 1000 ng/mL	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Human, Mouse	Dilution info Use at an assay dependent concentration.	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Transfected cell lysate - Human	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/5000	Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Species Mouse	Dilution info 1/5000	Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species	Dilution info	Notes
Species Transfected cell lysate - Human, Recombinant fragment - Human, Synthetic peptide - Human, Recombinant fragment - Mouse	Dilution info -	Notes -

Target data

See full target information Microtubule-associated protein tau

Function

Promotes microtubule assembly and stability, and might be involved in the establishment and maintenance of neuronal polarity (PubMed:21985311). The C-terminus binds axonal microtubules while the N-terminus binds neural plasma membrane components, suggesting that tau functions as a linker protein between both (PubMed:21985311, PubMed:32961270). Axonal polarity is predetermined by TAU/MAPT localization (in the neuronal cell) in the domain of the cell body defined by the centrosome. The short isoforms allow plasticity of the cytoskeleton whereas the longer isoforms may preferentially play a role in its stabilization.

Alternative names

MAPTL, MTBT1, TAU, MAPT, Microtubule-associated protein tau, Neurofibrillary tangle protein, Paired helical filament-tau, PHF-tau

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal
Immunogen: The exact immunogen used to generate this antibody is proprietary information.
Clone number: EPR29541-59
Purification technique: Affinity purification Protein A
Specificity: The antibody binds in the region surrounding Leu243 of 2N4R Tau.
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage duration: 1-2 weeks
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: -20°C
Aliquoting information: Upon delivery aliquot
Storage information: Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

11 product images

Indirect ELISA - Anti-Tau (MTBR-tau243) antibody [EPR29541-59] (ab322554)
Indirect ELISA analysis of ab322554 at 1000-0 ng/ml. The Secondary antibody used was Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1:2500 dilution dilution.
Antigen: Mouse PNS-Tau recombinant protein, Mouse Tau-A (corresponding to Human 2N4R) recombinant protein.
Antigen concentration: 1000 ng/ml
Indirect ELISA - Anti-Tau (MTBR-tau243) antibody [EPR29541-59] (ab322554)
Indirect ELISA analysis of ab322554 at 1000-0 ng/ml. The Secondary antibody used was Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1:2500 dilution dilution.
Antigen: Human Tau 2N4R peptide (surrounding Leu243), Human Tau 2N4R recombinant protein, Human MAP2 fragment.
Antigen concentration: 1000 ng/ml
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (MTBR-tau243) antibody [EPR29541-59] (ab322554)
Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue labeling Tau (MTBR domain) with ab322554 at 1/5000 (0.097 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Negative control: No staining in mouse spleen.

The section was incubated with ab322554 for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins
Western blot - Anti-Tau (MTBR-tau243) antibody [EPR29541-59] (ab322554)
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
This antibody does not cross-react with human MAP2 and MAP4.
The antibody binds in the region surrounding Leu243 of 2N4R Tau
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
In Western blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (Anti-6X His tag® antibody [EPR20547] - ChIP Grade ab213204) staining at 1/5000 dilution.
In Western blot, Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody(Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [EPR20018-251] ab205606) staining at 37-250KDa dilution.
All lanes: Western blot - Anti-Tau (MTBR-tau243) antibody [EPR29541-59] (ab322554) at 1/1000 dilution
Lane 1: 293T cells transfected with an empty vector containing a myc-His-tag®, whole cell lysate at 20 µg
Lane 2: 293T cells transfected with a human wild-type Tau 2N4R expression vector containing a myc-His-tag®, whole cell lysate at 20 µg
Lane 3: 293T cells transfected with a human wild-type PNS-Tau expression vector containing a DDDDK-tag, whole cell lysate at 20 µg
Lane 4: 293T cells transfected with a human wild-type MAP2 expression vector containing a DDDDK-tag, whole cell lysate at 20 µg
Lane 5: 293T cells transfected with a human wild-type MAP4 expression vector containing a DDDDK-tag, whole cell lysate at 20 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 33-100 kDa, 36 kDa, 15-150 kDa, 37-250 kDa
Exposure time: 6s
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (MTBR-tau243) antibody [EPR29541-59] (ab322554)
Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling Tau (MTBR domain) with ab322554 at 1/5000 (0.097 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Negative control: No staining in mouse liver.

The section was incubated with ab322554 for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (MTBR-tau243) antibody [EPR29541-59] (ab322554)
Immunohistochemical analysis of paraffin-embedded Human spleen tissue labeling Tau (MTBR domain) with ab322554 at 1/5000 (0.097 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Negative control: No staining in human spleen.

The section was incubated with ab322554 for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (MTBR-tau243) antibody [EPR29541-59] (ab322554)
Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling Tau (MTBR domain) with ab322554 at 1/5000 (0.097 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Negative control: No staining in human liver.

The section was incubated with ab322554 for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (MTBR-tau243) antibody [EPR29541-59] (ab322554)
Immunohistochemical analysis of paraffin-embedded (A) Mouse cerebrum. (B) Mouse Alzheimer's disease brain. tissue labeling Tau (MTBR domain) with ab322554 at 1/5000 (0.097 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining in (A) mouse cerebrum and (B) mouse Alzheimer's disease brain.

The section was incubated with ab322554 for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (MTBR-tau243) antibody [EPR29541-59] (ab322554)
Immunohistochemical analysis of paraffin-embedded (A) Human cerebrum. (B) Human Alzheimer's disease brain. tissue labeling Tau (MTBR domain) with ab322554 at 1/5000 (0.097 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining in (A) human cerebrum and (B) human Alzheimer's disease brain.

The section was incubated with ab322554 for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins
Western blot - Anti-Tau (MTBR-tau243) antibody [EPR29541-59] (ab322554)
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Negative control: EL4.
The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 24116116, PMID: 37443334, PMID:24386422).
In Western blot, Anti-Vinculin antibody [EPR8185] (Anti-Vinculin antibody [EPR8185] ab129002) staining at 1/10000 dilution.
All lanes: Western blot - Anti-Tau (MTBR-tau243) antibody [EPR29541-59] (ab322554) at 1/1000 dilution
Lane 1: Mouse primary neuron whole cell lysate at 50 µg
Lane 2: EL4 (mouse lymphoma T lymphocyte) whole cell lysate at 50 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 33-60 kDa, 124 kDa
Exposure time: 15s
Western blot - Anti-Tau (MTBR-tau243) antibody [EPR29541-59] (ab322554)
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Negative control: liver, spleen, testis, kidney (PMID:24386422).
The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 24116116, PMID: 37443334, PMID:24386422).
In Western blot, Anti-Vinculin antibody [EPR8185] (Anti-Vinculin antibody [EPR8185] ab129002) staining at 1/10000 dilution.
All lanes: Western blot - Anti-Tau (MTBR-tau243) antibody [EPR29541-59] (ab322554) at 1/1000 dilution
Lane 1: Human Alzheimers brain tissue lysate at 50 µg
Lane 2: Human cerebellum tissue lysate at 50 µg
Lane 3: human hippocampus tissue lysate at 50 µg
Lane 4: Human spleen tissue lysate at 50 µg
Lane 5: Human testis tissue lysate at 50 µg
Lane 6: Human liver tissue lysate at 50 µg
Lane 7: Mouse Alzheimers brain tissue lysate at 50 µg
Lane 8: Mouse cerebral cortex tissue lysate at 50 µg
Lane 9: Mouse hippocampus tissue lysate at 50 µg
Lane 10: Mouse cerebellum tissue lysate at 50 µg
Lane 11: Mouse brain tissue lysate at 50 µg
Lane 12: Mouse spleen tissue lysate at 50 µg
Lane 13: Mouse testis tissue lysate at 50 µg
Lane 14: Mouse liver tissue lysate at 50 µg
Lane 15: Mouse kidney tissue lysate at 50 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 33-60 kDa, 124 kDa
Exposure time: 26s