Anti-Tau (MTBR-tau243) antibody [EPR29541-59] - BSA and Azide free

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (MTBR-tau243) antibody [EPR29541-59] - BSA and Azide free (AB323528)

This data was developed using ab322554, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded (A) Human cerebrum. (B) Human Alzheimer's disease brain. tissue labeling Tau (MTBR domain) with ab322554 at 1/5000 (0.097 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining in (A) human cerebrum and (B) human Alzheimer's disease brain.
The section was incubated with ab322554 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0 Epitope Retrieval Solution 1) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (MTBR-tau243) antibody [EPR29541-59] - BSA and Azide free (AB323528)

This data was developed using ab322554, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded (A) Mouse cerebrum. (B) Mouse Alzheimer's disease brain. tissue labeling Tau (MTBR domain) with ab322554 at 1/5000 (0.097 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining in (A) mouse cerebrum and (B) mouse Alzheimer's disease brain.
The section was incubated with ab322554 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0 Epitope Retrieval Solution 1) for 20 mins

• WB

Supplier Data

Western blot - Anti-Tau (MTBR-tau243) antibody [EPR29541-59] - BSA and Azide free (AB323528)

This data was developed using ab322554, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Negative control : liver spleen testis kidney (PMID : 24386422).

The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID : 24116116 PMID : 37443334 PMID : 24386422).

In Western blot Anti-Vinculin antibody [EPR8185] (ab129002) staining at 1/10000 dilution.

All lanes:

Western blot - Anti-Tau (MTBR-tau243) antibody [EPR29541-59] (<a href='/en-us/products/primary-antibodies/tau-mtbr-tau243-antibody-epr29541-59-ab322554'>ab322554</a>) at 1/1000 dilution

Lane 1:

Human Alzheimers brain tissue lysate at 50 µg

Lane 2:

Human cerebellum tissue lysate at 50 µg

Lane 3:

human hippocampus tissue lysate at 50 µg

Lane 4:

Human spleen tissue lysate at 50 µg

Lane 5:

Human testis tissue lysate at 50 µg

Lane 6:

Human liver tissue lysate at 50 µg

Lane 7:

Mouse Alzheimers brain tissue lysate at 50 µg

Lane 8:

Mouse cerebral cortex tissue lysate at 50 µg

Lane 9:

Mouse hippocampus tissue lysate at 50 µg

Lane 10:

Mouse cerebellum tissue lysate at 50 µg

Lane 11:

Mouse brain tissue lysate at 50 µg

Lane 12:

Mouse spleen tissue lysate at 50 µg

Lane 13:

Mouse testis tissue lysate at 50 µg

Lane 14:

Mouse liver tissue lysate at 50 µg

Lane 15:

Mouse kidney tissue lysate at 50 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 33-60 kDa,124 kDa

false

Exposure time: 26s

• WB

Supplier Data

Western blot - Anti-Tau (MTBR-tau243) antibody [EPR29541-59] - BSA and Azide free (AB323528)

This data was developed using ab322554, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Negative control : EL4.

The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID : 24116116 PMID : 37443334 PMID : 24386422).

In Western blot Anti-Vinculin antibody [EPR8185] (ab129002) staining at 1/10000 dilution.

All lanes:

Western blot - Anti-Tau (MTBR-tau243) antibody [EPR29541-59] (<a href='/en-us/products/primary-antibodies/tau-mtbr-tau243-antibody-epr29541-59-ab322554'>ab322554</a>) at 1/1000 dilution

Lane 1:

Mouse primary neuron whole cell lysate at 50 µg

Lane 2:

EL4 (mouse lymphoma T lymphocyte) whole cell lysate at 50 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 33-60 kDa,124 kDa

false

Exposure time: 15s

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (MTBR-tau243) antibody [EPR29541-59] - BSA and Azide free (AB323528)

This data was developed using ab322554, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human spleen tissue labeling Tau (MTBR domain) with ab322554 at 1/5000 (0.097 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Negative control : No staining in human spleen.
The section was incubated with ab322554 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0 Epitope Retrieval Solution 1) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (MTBR-tau243) antibody [EPR29541-59] - BSA and Azide free (AB323528)

This data was developed using ab322554, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling Tau (MTBR domain) with ab322554 at 1/5000 (0.097 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Negative control : No staining in mouse liver.
The section was incubated with ab322554 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0 Epitope Retrieval Solution 1) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (MTBR-tau243) antibody [EPR29541-59] - BSA and Azide free (AB323528)

This data was developed using ab322554, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling Tau (MTBR domain) with ab322554 at 1/5000 (0.097 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Negative control : No staining in human liver.
The section was incubated with ab322554 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0 Epitope Retrieval Solution 1) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (MTBR-tau243) antibody [EPR29541-59] - BSA and Azide free (AB323528)

This data was developed using ab322554, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue labeling Tau (MTBR domain) with ab322554 at 1/5000 (0.097 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Negative control : No staining in mouse spleen.
The section was incubated with ab322554 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0 Epitope Retrieval Solution 1) for 20 mins

• I-ELISA

Supplier Data

Indirect ELISA - Anti-Tau (MTBR-tau243) antibody [EPR29541-59] - BSA and Azide free (AB323528)

This data was developed using ab322554, the same antibody clone in a different buffer formulation.

Indirect ELISA analysis of ab322554 at 1000-0 ng/ml. The Secondary antibody used was Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1 : 2500 dilution dilution.

Antigen : Mouse PNS-Tau recombinant protein, Mouse Tau-A (corresponding to Human 2N4R) recombinant protein.

Antigen concentration : 1000 ng/ml

• I-ELISA

Supplier Data

Indirect ELISA - Anti-Tau (MTBR-tau243) antibody [EPR29541-59] - BSA and Azide free (AB323528)

This data was developed using ab322554, the same antibody clone in a different buffer formulation.

Indirect ELISA analysis of ab322554 at 1000-0 ng/ml. The Secondary antibody used was Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1 : 2500 dilution dilution.

Antigen : Human Tau 2N4R peptide (surrounding Leu243), Human Tau 2N4R recombinant protein, Human MAP2 fragment.

Antigen concentration : 1000 ng/ml

• WB

Supplier Data

Western blot - Anti-Tau (MTBR-tau243) antibody [EPR29541-59] - BSA and Azide free (AB323528)

This data was developed using ab322554, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

This antibody does not cross-react with human MAP2 and MAP4.

The antibody binds in the region surrounding Leu243 of 2N4R Tau

In Western blot Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

In Western blot Anti-6X His tag® antibody [EPR20547] - ChIP Grade (ab213204) staining at 1/5000 dilution.

In Western blot Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody(ab205606) staining at 37-250KDa dilution.

All lanes:

Western blot - Anti-Tau (MTBR-tau243) antibody [EPR29541-59] (<a href='/en-us/products/primary-antibodies/tau-mtbr-tau243-antibody-epr29541-59-ab322554'>ab322554</a>) at 1/1000 dilution

Lane 1:

293T cells transfected with an empty vector containing a myc-His-tag®, whole cell lysate at 20 µg

Lane 2:

293T cells transfected with a human wild-type Tau 2N4R expression vector containing a myc-His-tag®, whole cell lysate at 20 µg

Lane 3:

293T cells transfected with a human wild-type PNS-Tau expression vector containing a DDDDK-tag, whole cell lysate at 20 µg

Lane 4:

293T cells transfected with a human wild-type MAP2 expression vector containing a DDDDK-tag, whole cell lysate at 20 µg

Lane 5:

293T cells transfected with a human wild-type MAP4 expression vector containing a DDDDK-tag, whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 33-100 kDa,36 kDa,15-150 kDa,37-250 kDa

false

Exposure time: 6s