Anti-Tau (N-terminal) antibody [EPR29521-8] (ab324236)

Rabbit Recombinant Monoclonal TAU antibody. Suitable for WB, I-ELISA, IHC-P and reacts with Transfected cell lysate - Human, Human, Mouse, Recombinant full length protein - Mouse, Recombinant full length protein - Human, Synthetic peptide - Human samples.

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Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (N-terminal) antibody [EPR29521-8] (AB324236), expandable thumbnail

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	WB	I-ELISA	IHC-P
Human	Tested	Expected	Tested
Mouse	Tested	Expected	Tested
Recombinant full length protein - Human	Not recommended	Tested	Not recommended
Recombinant full length protein - Mouse	Not recommended	Tested	Not recommended
Synthetic peptide - Human	Not recommended	Tested	Not recommended
Transfected cell lysate - Human	Tested	Not recommended	Not recommended

Tested
Tested

Species	Dilution info	Notes
Species Transfected cell lysate - Human	Dilution info 1/1000	Notes -
Species Human	Dilution info 1/1000	Notes -
Species Mouse	Dilution info 1/1000	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Recombinant full length protein - Mouse, Recombinant full length protein - Human, Synthetic peptide - Human	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Recombinant full length protein - Mouse	Dilution info 31.25 ng/mL	Notes -
Species Recombinant full length protein - Human	Dilution info 31.25 ng/mL	Notes -
Species Synthetic peptide - Human	Dilution info 31.25 ng/mL	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Human, Mouse	Dilution info Use at an assay dependent concentration.	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Transfected cell lysate - Human	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/2000	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Mouse	Dilution info 1/2000	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species	Dilution info	Notes
Species Transfected cell lysate - Human, Recombinant full length protein - Mouse, Recombinant full length protein - Human, Synthetic peptide - Human	Dilution info -	Notes -

Target data

See full target information MAPT

Function

The protein expressed by the MAPT gene promotes microtubule assembly and stability and might be involved in establishing and maintaining neuronal polarity. Its C-terminus binds axonal microtubules, and the N-terminus binds neural plasma membrane components, suggesting that tau functions as a linker protein between the two. Axonal polarity is predetermined by MAPT localization within the neuronal cell's domain defined by the centrosome. The short isoforms allow cytoskeleton plasticity, whereas the longer isoforms may preferentially play a role in its stabilization. This supplementary information is collated from multiple sources and compiled automatically.

Alternative names

MAPTL, MTBT1, TAU, MAPT, Microtubule-associated protein tau, Neurofibrillary tangle protein, Paired helical filament-tau, PHF-tau

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal
Immunogen: The exact immunogen used to generate this antibody is proprietary information.
Clone number: EPR29521-8
Purification technique: Affinity purification Protein A
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage duration: 1-2 weeks
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: -20°C
Aliquoting information: Upon delivery aliquot
Storage information: Avoid freeze / thaw cycle

Notes

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

10 product images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (N-terminal) antibody [EPR29521-8] (ab324236)
Tau (N-terminal) Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining of Mouse cerebrum using rabbit Anti-Tau (N-terminal) antibody
Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling Tau (N-terminal) with ab324236 at 1/2000 (0.249 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining in mouse cerebrum.

The section was incubated for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Western blot - Anti-Tau (N-terminal) antibody [EPR29521-8] (ab324236)
Tau (N-terminal) Western blot staining using rabbit Anti-Tau (N-terminal) antibody
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Negative control: spleen, testis (PMID:24386422).
The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 24116116, PMID: 37443334, PMID:24386422).
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
All lanes: Western blot - Anti-Tau (N-terminal) antibody [EPR29521-8] (ab324236) at 1/1000 dilution
Lane 1: Human Alzheimer's brain tissue lysate at 20 µg
Lane 2: Human cerebellum tissue lysate at 20 µg
Lane 3: Human hippocampus tissue lysate at 20 µg
Lane 4: Human spleen tissue lysate at 20 µg
Lane 5: Human testis tissue lysate at 20 µg
Lane 6: Mouse cerebellum tissue lysate at 20 µg
Lane 7: Mouse brain tissue lysate at 20 µg
Lane 8: Mouse spleen tissue lysate at 20 µg
Lane 9: Mouse testis tissue lysate at 20 µg
Secondary
All lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Observed band size: 33-60 kDa, 36 kDa
Exposure time: 59s
Indirect ELISA - Anti-Tau (N-terminal) antibody [EPR29521-8] (ab324236)
Indirect ELISA analysis of ab324236 at 1000-0 ng/ml. The Secondary antibody used was Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1:2500 dilution dilution.
Antigen: Mouse PNS-Tau recombinant protein, Mouse Tau-A (corresponding to Human 2N4R) recombinant protein.
Antigen concentration: 1000 ng/ml
Indirect ELISA - Anti-Tau (N-terminal) antibody [EPR29521-8] (ab324236)
Indirect ELISA analysis of ab324236 at 1000-0 ng/ml. The Secondary antibody used was Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1:2500 dilution dilution.
Antigen: Human Tau 2N4R-N terminal peptide, Human Tau 2N4R recombinant protein, Human MAP2 fragment.
Antigen concentration: 1000 ng/ml
Western blot - Anti-Tau (N-terminal) antibody [EPR29521-8] (ab324236)
Tau (N-terminal) Western blot staining using rabbit Anti-Tau (N-terminal) antibody
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Negative control: EL4.
The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 24116116, PMID: 37443334, PMID:24386422).
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
All lanes: Western blot - Anti-Tau (N-terminal) antibody [EPR29521-8] (ab324236) at 1/1000 dilution
Lane 1: Mouse primary neuron whole cell lysate at 50 µg
Lane 2: EL4 (mouse lymphoma T lymphocyte) whole cell lysate at 50 µg
Secondary
All lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/20000 dilution
Observed band size: 33-60 kDa, 36 kDa
Exposure time: 59s
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (N-terminal) antibody [EPR29521-8] (ab324236)
Tau (N-terminal) Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining of Human cerebrum using rabbit Anti-Tau (N-terminal) antibody
Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labeling Tau (N-terminal) with ab324236 at 1/2000 (0.249 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining in human cerebrum.

The section was incubated for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (N-terminal) antibody [EPR29521-8] (ab324236)
Tau (N-terminal) Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining of Human alzheimer's disease (AD) brain using rabbit Anti-Tau (N-terminal) antibody
Immunohistochemical analysis of paraffin-embedded Human alzheimer's disease (AD) brain tissue labeling Tau (N-terminal) with ab324236 at 1/2000 (0.249 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining in human AD brain.

The section was incubated for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (N-terminal) antibody [EPR29521-8] (ab324236)
Tau (N-terminal) Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining of Human spleen using rabbit Anti-Tau (N-terminal) antibody
Immunohistochemical analysis of paraffin-embedded Human spleen tissue labeling Tau (N-terminal) with ab324236 at 1/2000 (0.249 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Negative control: No staining in human spleen.

The section was incubated for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (N-terminal) antibody [EPR29521-8] (ab324236)
Tau (N-terminal) Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining of Mouse spleen using rabbit Anti-Tau (N-terminal) antibody
Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue labeling Tau (N-terminal) with ab324236 at 1/2000 (0.249 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Negative control: No staining in mouse spleen (PMID:24386422).

The section was incubated for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Western blot - Anti-Tau (N-terminal) antibody [EPR29521-8] (ab324236)
Tau (N-terminal) Western blot staining using rabbit Anti-Tau (N-terminal) antibody
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
This antibody does not cross-react with human MAP2 and MAP4.
In Western blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (Anti-6X His tag® antibody [EPR20547] - ChIP Grade ab213204) staining at 1/5000 dilution.
All lanes: Western blot - Anti-Tau (N-terminal) antibody [EPR29521-8] (ab324236) at 1/1000 dilution
Lane 1: 293T cells transfected with an empty vector containing a myc-His-tag®, whole cell lysate at 20 µg
Lane 2: 293T cells transfected with a human Tau 2N4R expression vector containing a myc-His-tag®, whole cell lysate at 20 µg
Lane 3: 293T cells transfected with a human PNS-Tau expression vector containing a DDDDK-tag, whole cell lysate at 20 µg
Lane 4: 293T cells transfected with a human MAP2 expression vector containing a DDDDK-tag, whole cell lysate at 20 µg
Lane 5: 293T cells transfected with a human MAP4 expression vector containing a DDDDK-tag, whole cell lysate at 20 µg
Secondary
All lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/100000 dilution
Observed band size: 33-60 kDa, 15-150 kDa, 37-250 kDa, 36 kDa
Exposure time: 1s