Anti-Tau (N-terminal) antibody [EPR29521-8]

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (N-terminal) antibody [EPR29521-8] (AB324236)

Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labeling Tau (N-terminal) with ab324236 at 1/2000 (0.249 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining in human cerebrum.
The section was incubated for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (N-terminal) antibody [EPR29521-8] (AB324236)

Immunohistochemical analysis of paraffin-embedded Human spleen tissue labeling Tau (N-terminal) with ab324236 at 1/2000 (0.249 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Negative control : No staining in human spleen.
The section was incubated for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (N-terminal) antibody [EPR29521-8] (AB324236)

Immunohistochemical analysis of paraffin-embedded Human alzheimer's disease (AD) brain tissue labeling Tau (N-terminal) with ab324236 at 1/2000 (0.249 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining in human AD brain.
The section was incubated for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (N-terminal) antibody [EPR29521-8] (AB324236)

Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue labeling Tau (N-terminal) with ab324236 at 1/2000 (0.249 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Negative control : No staining in mouse spleen (PMID : 24386422).
The section was incubated for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (N-terminal) antibody [EPR29521-8] (AB324236)

Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling Tau (N-terminal) with ab324236 at 1/2000 (0.249 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining in mouse cerebrum.
The section was incubated for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• WB

Supplier Data

Western blot - Anti-Tau (N-terminal) antibody [EPR29521-8] (AB324236)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Negative control : spleen, testis (PMID : 24386422).

The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID : 24116116, PMID : 37443334, PMID : 24386422).

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-Tau (N-terminal) antibody [EPR29521-8] (ab324236) at 1/1000 dilution

Lane 1:

Human Alzheimer's brain tissue lysate at 20 µg

Lane 2:

Human cerebellum tissue lysate at 20 µg

Lane 3:

Human hippocampus tissue lysate at 20 µg

Lane 4:

Human spleen tissue lysate at 20 µg

Lane 5:

Human testis tissue lysate at 20 µg

Lane 6:

Mouse cerebellum tissue lysate at 20 µg

Lane 7:

Mouse brain tissue lysate at 20 µg

Lane 8:

Mouse spleen tissue lysate at 20 µg

Lane 9:

Mouse testis tissue lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Observed band size: 33-60 kDa,36 kDa

false

Exposure time: 59s

• WB

Supplier Data

Western blot - Anti-Tau (N-terminal) antibody [EPR29521-8] (AB324236)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Negative control : EL4.

The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID : 24116116, PMID : 37443334, PMID : 24386422).

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-Tau (N-terminal) antibody [EPR29521-8] (ab324236) at 1/1000 dilution

Lane 1:

Mouse primary neuron whole cell lysate at 50 µg

Lane 2:

EL4 (mouse lymphoma T lymphocyte) whole cell lysate at 50 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/20000 dilution

Observed band size: 33-60 kDa,36 kDa

false

Exposure time: 59s

• I-ELISA

Supplier Data

Indirect ELISA - Anti-Tau (N-terminal) antibody [EPR29521-8] (AB324236)

Indirect ELISA analysis of ab324236 at 1000-0 ng/ml. The Secondary antibody used was Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1 : 2500 dilution dilution.

Antigen : Mouse PNS-Tau recombinant protein, Mouse Tau-A (corresponding to Human 2N4R) recombinant protein.

Antigen concentration : 1000 ng/ml

• I-ELISA

Supplier Data

Indirect ELISA - Anti-Tau (N-terminal) antibody [EPR29521-8] (AB324236)

Indirect ELISA analysis of ab324236 at 1000-0 ng/ml. The Secondary antibody used was Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1 : 2500 dilution dilution.

Antigen : Human Tau 2N4R-N terminal peptide, Human Tau 2N4R recombinant protein, Human MAP2 fragment.

Antigen concentration : 1000 ng/ml

• WB

Supplier Data

Western blot - Anti-Tau (N-terminal) antibody [EPR29521-8] (AB324236)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

This antibody does not cross-react with human MAP2 and MAP4.

In Western blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (ab213204) staining at 1/5000 dilution.

All lanes:

Western blot - Anti-Tau (N-terminal) antibody [EPR29521-8] (ab324236) at 1/1000 dilution

Lane 1:

293T cells transfected with an empty vector containing a myc-His-tag®, whole cell lysate at 20 µg

Lane 2:

293T cells transfected with a human Tau 2N4R expression vector containing a myc-His-tag®, whole cell lysate at 20 µg

Lane 3:

293T cells transfected with a human PNS-Tau expression vector containing a DDDDK-tag, whole cell lysate at 20 µg

Lane 4:

293T cells transfected with a human MAP2 expression vector containing a DDDDK-tag, whole cell lysate at 20 µg

Lane 5:

293T cells transfected with a human MAP4 expression vector containing a DDDDK-tag, whole cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/100000 dilution

Observed band size: 33-60 kDa,15-150 kDa,37-250 kDa,36 kDa

false

Exposure time: 1s