Rabbit Recombinant Monoclonal TAU nitrosyl Y29 antibody. Suitable for WB, I-ELISA, Dot and reacts with Transfected cell lysate - Human, Synthetic peptide - Human samples.
Images
![Dot Blot - Anti-Tau (nitrosyl Y29) antibody [EPR27841-79] (AB315891), expandable thumbnail](https://content.abcam.com/products/images/tau-nitrosyl-y29-antibody-epr27841-79-ab315891--dot-blot-img426319.jpg/_jcr_content/renditions/webp-475.webp "Dot Blot - Anti-Tau (nitrosyl Y29) antibody [EPR27841-79] (AB315891)")
![Western blot - Anti-Tau (nitrosyl Y29) antibody [EPR27841-79] (AB315891), expandable thumbnail](https://content.abcam.com/products/images/tau-nitrosyl-y29-antibody-epr27841-79-ab315891--western-blot-img426309.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Tau (nitrosyl Y29) antibody [EPR27841-79] (AB315891)")
![Indirect ELISA - Anti-Tau (nitrosyl Y29) antibody [EPR27841-79] (AB315891), expandable thumbnail](https://content.abcam.com/products/images/tau-nitrosyl-y29-antibody-epr27841-79-ab315891--indirect-elisa-img426367.jpg/_jcr_content/renditions/webp-475.webp "Indirect ELISA - Anti-Tau (nitrosyl Y29) antibody [EPR27841-79] (AB315891)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | I-ELISA | Dot | IHC-P | IHC-Fr | IP | ICC/IF | Flow Cyt (Intra) | |
|---|---|---|---|---|---|---|---|---|
| Human | Predicted | Predicted | Predicted | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
| Mouse | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
| Rat | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
| Synthetic peptide - Human | Not recommended | Tested | Tested | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
| Transfected cell lysate - Human | Tested | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell lysate - Human | Dilution info 1/1000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide - Human, Mouse, Rat | Dilution info - | Notes - |
I-ELISA
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide - Human | Dilution info 1000 ng/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell lysate - Human, Mouse, Rat | Dilution info - | Notes - |
Dot
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide - Human | Dilution info 1/1000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell lysate - Human, Mouse, Rat | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat, Transfected cell lysate - Human, Synthetic peptide - Human | Dilution info - | Notes - |
IHC-Fr
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human, Transfected cell lysate - Human, Synthetic peptide - Human, Rat | Dilution info - | Notes - |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Transfected cell lysate - Human, Synthetic peptide - Human, Mouse, Rat | Dilution info - | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Transfected cell lysate - Human, Synthetic peptide - Human, Mouse, Rat | Dilution info - | Notes - |
Flow Cyt (Intra)
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Transfected cell lysate - Human, Synthetic peptide - Human, Mouse, Rat | Dilution info - | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Version
Target data
Function
The protein expressed by the MAPT gene promotes microtubule assembly and stability and might be involved in establishing and maintaining neuronal polarity. Its C-terminus binds axonal microtubules, and the N-terminus binds neural plasma membrane components, suggesting that tau functions as a linker protein between the two. Axonal polarity is predetermined by MAPT localization within the neuronal cell's domain defined by the centrosome. The short isoforms allow cytoskeleton plasticity, whereas the longer isoforms may preferentially play a role in its stabilization. This supplementary information is collated from multiple sources and compiled automatically.
Alternative names
MAPTL, MTBT1, TAU, MAPT, Microtubule-associated protein tau, Neurofibrillary tangle protein, Paired helical filament-tau, PHF-tau
Recommended products
Rabbit Recombinant Monoclonal TAU nitrosyl Y29 antibody. Suitable for WB, I-ELISA, Dot and reacts with Transfected cell lysate - Human, Synthetic peptide - Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR27841-79
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Tau also known as microtubule-associated protein Tau (MAPT) plays an important role in stabilizing microtubules in neuronal cells. Tau is primarily found in the central nervous system but also exists in peripheral neurons. Human Tau protein comes in six isoforms due to alternative splicing with molecular weights ranging from 48 kDa to 67 kDa. This protein predominantly locates in the axons of neurons where it maintains the stability of microtubule tracks necessary for axonal transport.
- Biological function summary
Tau is involved in the assembly and stabilization of microtubules essential for maintaining neuronal structure. It interacts with microtubule-binding domains (MBD) to bind and bundle microtubules facilitating intracellular transport. Tau forms a part of the neuronal cytoskeleton complex working closely with other cytoskeletal proteins to preserve the proper axonal transport and function. Abnormally phosphorylated Tau often termed phospho-Tau disrupts this complex affecting microtubule stability.
- Pathways
Tau has critical involvement in several signaling cascades such as the microtubule-binding and transport pathways. Glycogen synthase kinase 3 beta (GSK3β) and cyclin-dependent kinase 5 (CDK5) frequently phosphorylate Tau controlling its interaction with microtubules. Phosphorylated Tau accumulates leading to the formation of neurofibrillary tangles often observed in neurodegenerative conditions. Additionally Tau interacts with GAPDH impacting cellular energy regulation through potential pathway cross-talk involving oxidative stress responses.
- Associated diseases and disorders
Tau is closely associated with Alzheimer's disease and frontotemporal dementia. In Alzheimer's disease hyperphosphorylated Tau aggregates into paired helical filaments forming neurofibrillary tangles while similar aggregates are observed in frontotemporal dementia. In these conditions Tau links to amyloid precursor protein (APP) where misregulated phosphorylation-driven interactions contribute to neurodegeneration. Identifying phospho-Tau and its altered interactions with related proteins aids in understanding and potentially treating these disorders.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
![Dot Blot - Anti-Tau (nitrosyl Y29) antibody [EPR27841-79] (ab315891), expandable thumbnail](https://content.abcam.com/products/images/tau-nitrosyl-y29-antibody-epr27841-79-ab315891--dot-blot-img426319.jpg "Dot Blot - Anti-Tau (nitrosyl Y29) antibody [EPR27841-79] (ab315891)")
Dot Blot - Anti-Tau (nitrosyl Y29) antibody [EPR27841-79] (ab315891)
Tau (nitrosyl Y29) Dot Blot staining using rabbit Anti-Tau (nitrosyl Y29) antibody
Dot blot analysis of Tau (nitrosyl Y29) using ab315891 at 1:1000 (0.518 ug/ml) followed by a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1:100,000 dilution.
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
All lanes: Dot Blot - Anti-Tau (nitrosyl Y29) antibody [EPR27841-79] (ab315891) at 1/1000 dilution
Lane 1: Tau (nitrosylated Y29) peptide a
Lane 2: Tau (nitrosylated Y29) peptide b
Lane 3: Tau non-nitrosylated Y29 peptide c
Lane 4: Tau (phospho Y29) peptide d
Lane 5: Tau (nitrosylated Y18) peptide e
Lane 6: Tau (nitrosylated Y18) peptide f
SecondaryAll lanes: Dot Blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Exposure time: 180s
![Western blot - Anti-Tau (nitrosyl Y29) antibody [EPR27841-79] (ab315891), expandable thumbnail](https://content.abcam.com/products/images/tau-nitrosyl-y29-antibody-epr27841-79-ab315891--western-blot-img426309.jpg "Western blot - Anti-Tau (nitrosyl Y29) antibody [EPR27841-79] (ab315891)")
Western blot - Anti-Tau (nitrosyl Y29) antibody [EPR27841-79] (ab315891)
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
In Western blot, Anti-Tau antibody - Total protein control (Anti-Tau antibody [EPR22524-95] ab254256) staining at 1/1000 dilution.
In Western blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (Anti-6X His tag® antibody [EPR20547] - ChIP Grade ab213204) staining at 1/5000 dilution.
All lanes: Western blot - Anti-Tau (nitrosyl Y29) antibody [EPR27841-79] (ab315891) at 1/1000 dilution
Lane 1: Untreated 293T cells transfected with an empty vector containing a His-tag, whole cell lysate at 20 µg with 5% NFDM/TBST
Lane 2: 293T cells transfected with an empty vector containing a His-tag were treated with 3mM peroxynitrite for 1 hour, whole cell lysate at 20 µg with 5% NFDM/TBST
Lane 3: Untreated 293T cells transfected with a human Tau 2N4R expression vector containing a His-tag, whole cell lysate at 20 µg with 5% NFDM/TBST
Lane 4: 293T cells transfected with a human Tau 2N4R expression vector containing a His-tag were treated with 3mM peroxynitrite for 1 hour, whole cell lysate at 20 µg with 5% NFDM/TBST
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 20-75 kDa, 36 kDa
Exposure time: 180s
![Indirect ELISA - Anti-Tau (nitrosyl Y29) antibody [EPR27841-79] (ab315891), expandable thumbnail](https://content.abcam.com/products/images/tau-nitrosyl-y29-antibody-epr27841-79-ab315891--indirect-elisa-img426367.jpg "Indirect ELISA - Anti-Tau (nitrosyl Y29) antibody [EPR27841-79] (ab315891)")
Indirect ELISA - Anti-Tau (nitrosyl Y29) antibody [EPR27841-79] (ab315891)
Indirect ELISA analysis of ab315891 at 1000-0 ng/ml. The Secondary antibody used was Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1:2500 dilution.
Antigen: Tau (nitrosylated Y29) peptide a,Tau (nitrosylated Y29) peptide b,Tau non-nitrosylated Y29 peptide c,Tau (phospho Y29) peptide d,Tau (nitrosylated Y18) peptide e,Tau (nitrosylated Y18) peptide f.
Antigen concentration: 1000 ng/ml
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