Rabbit Recombinant Monoclonal TAU glcnac S400 antibody. Carrier free. Suitable for WB, ICC/IF and reacts with Transfected cell lysate - Human, Transfected cell line - Human samples.
Images
![Western blot - Anti-Tau (O-GlcNAc S400) antibody [EPR24891-48] - BSA and Azide free (AB320077), expandable thumbnail](https://content.abcam.com/products/images/tau-o-glcnac-s400-antibody-epr24891-48-bsa-and-azide-free-ab320077--western-blot-img435164.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Tau (O-GlcNAc S400) antibody [EPR24891-48] - BSA and Azide free (AB320077)")
![Western blot - Anti-Tau (O-GlcNAc S400) antibody [EPR24891-48] - BSA and Azide free (AB320077), expandable thumbnail](https://content.abcam.com/products/images/tau-o-glcnac-s400-antibody-epr24891-48-bsa-and-azide-free-ab320077--western-blot-img435163.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Tau (O-GlcNAc S400) antibody [EPR24891-48] - BSA and Azide free (AB320077)")
![Immunocytochemistry/ Immunofluorescence - Anti-Tau (O-GlcNAc S400) antibody [EPR24891-48] - BSA and Azide free (AB320077), expandable thumbnail](https://content.abcam.com/products/images/tau-o-glcnac-s400-antibody-epr24891-48-bsa-and-azide-free-ab320077--immunocytochemistry-immunofluorescence-img435185.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-Tau (O-GlcNAc S400) antibody [EPR24891-48] - BSA and Azide free (AB320077)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | ICC/IF | |
|---|---|---|
| Human | Predicted | Predicted |
| Transfected cell line - Human | Not recommended | Tested |
| Transfected cell lysate - Human | Tested | Not recommended |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell lysate - Human | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell line - Human | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell line - Human | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell lysate - Human | Dilution info - | Notes - |
Target data
Function
Promotes microtubule assembly and stability, and might be involved in the establishment and maintenance of neuronal polarity (PubMed:21985311). The C-terminus binds axonal microtubules while the N-terminus binds neural plasma membrane components, suggesting that tau functions as a linker protein between both (PubMed:21985311, PubMed:32961270). Axonal polarity is predetermined by TAU/MAPT localization (in the neuronal cell) in the domain of the cell body defined by the centrosome. The short isoforms allow plasticity of the cytoskeleton whereas the longer isoforms may preferentially play a role in its stabilization.
Alternative names
MAPTL, MTBT1, TAU, Microtubule-associated protein tau, Neurofibrillary tangle protein, Paired helical filament-tau, PHF-tau
Recommended products
Rabbit Recombinant Monoclonal TAU glcnac S400 antibody. Carrier free. Suitable for WB, ICC/IF and reacts with Transfected cell lysate - Human, Transfected cell line - Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogens
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR24891-48
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
Notes
ab320077 is the carrier-free version of Anti-Tau (O-GlcNAc S400) antibody [EPR24891-48] ab320076.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Tau also known as microtubule-associated protein Tau (MAPT) plays an important role in stabilizing microtubules in neuronal cells. Tau is primarily found in the central nervous system but also exists in peripheral neurons. Human Tau protein comes in six isoforms due to alternative splicing with molecular weights ranging from 48 kDa to 67 kDa. This protein predominantly locates in the axons of neurons where it maintains the stability of microtubule tracks necessary for axonal transport.
- Biological function summary
Tau is involved in the assembly and stabilization of microtubules essential for maintaining neuronal structure. It interacts with microtubule-binding domains (MBD) to bind and bundle microtubules facilitating intracellular transport. Tau forms a part of the neuronal cytoskeleton complex working closely with other cytoskeletal proteins to preserve the proper axonal transport and function. Abnormally phosphorylated Tau often termed phospho-Tau disrupts this complex affecting microtubule stability.
- Pathways
Tau has critical involvement in several signaling cascades such as the microtubule-binding and transport pathways. Glycogen synthase kinase 3 beta (GSK3β) and cyclin-dependent kinase 5 (CDK5) frequently phosphorylate Tau controlling its interaction with microtubules. Phosphorylated Tau accumulates leading to the formation of neurofibrillary tangles often observed in neurodegenerative conditions. Additionally Tau interacts with GAPDH impacting cellular energy regulation through potential pathway cross-talk involving oxidative stress responses.
- Associated diseases and disorders
Tau is closely associated with Alzheimer's disease and frontotemporal dementia. In Alzheimer's disease hyperphosphorylated Tau aggregates into paired helical filaments forming neurofibrillary tangles while similar aggregates are observed in frontotemporal dementia. In these conditions Tau links to amyloid precursor protein (APP) where misregulated phosphorylation-driven interactions contribute to neurodegeneration. Identifying phospho-Tau and its altered interactions with related proteins aids in understanding and potentially treating these disorders.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
![Western blot - Anti-Tau (O-GlcNAc S400) antibody [EPR24891-48] - BSA and Azide free (ab320077), expandable thumbnail](https://content.abcam.com/products/images/tau-o-glcnac-s400-antibody-epr24891-48-bsa-and-azide-free-ab320077--western-blot-img435164.jpg "Western blot - Anti-Tau (O-GlcNAc S400) antibody [EPR24891-48] - BSA and Azide free (ab320077)")
Western blot - Anti-Tau (O-GlcNAc S400) antibody [EPR24891-48] - BSA and Azide free (ab320077)
This data was developed using Anti-Tau (O-GlcNAc S400) antibody [EPR24891-48] ab320076, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
The expression of Tau (O-GlcNAc S400) is reduced following deglycosylation induced by PNGase F.
The identity of the higher MW band at approximately 110 kDa is unknown.
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
All lanes: Western blot - Anti-Tau (O-GlcNAc S400) antibody [EPR24891-48] (Anti-Tau (O-GlcNAc S400) antibody [EPR24891-48] ab320076) at 1/1000 dilution
Lane 1: 293T cells transfected with a human wild-type Tau 2N4R expression vector containing a myc-His-tag® were treated with 10 uM Thiamet G for 48 hours whole cell lysate at 20 µg
Lane 2: 293T cells transfected with a human wild-type Tau 2N4R expression vector containing a myc-His-tag® were treated with 10 uM Thiamet G for 48 hours whole cell lysate treated with Peptide:N-glycosidase F (PNGase F) at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Observed band size: 25-75 kDa, 36 kDa
Exposure time: 92s
![Western blot - Anti-Tau (O-GlcNAc S400) antibody [EPR24891-48] - BSA and Azide free (ab320077), expandable thumbnail](https://content.abcam.com/products/images/tau-o-glcnac-s400-antibody-epr24891-48-bsa-and-azide-free-ab320077--western-blot-img435163.jpg "Western blot - Anti-Tau (O-GlcNAc S400) antibody [EPR24891-48] - BSA and Azide free (ab320077)")
Western blot - Anti-Tau (O-GlcNAc S400) antibody [EPR24891-48] - BSA and Azide free (ab320077)
This data was developed using Anti-Tau (O-GlcNAc S400) antibody [EPR24891-48] ab320076, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 22366723, PMID: 24113653).
The expression of Tau (O-GlcNAc S400) is upregulated in response to Thiamet G treatment (PMID: 25336656, PMID: 22366723, PMID: 24113653).
The identity of the higher MW band at approximately 110 kDa is unknown.
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
In Western blot, Anti-Tau antibody - Total protein control (Anti-Tau antibody [EP2456Y] ab76128) staining at 1/1000 dilution.
All lanes: Western blot - Anti-Tau (O-GlcNAc S400) antibody [EPR24891-48] (Anti-Tau (O-GlcNAc S400) antibody [EPR24891-48] ab320076) at 1/1000 dilution
Lane 1: 293T (human embryonic kidney epithelial cell) cells transfected with a human wild-type Tau 2N4R expression vector containing a myc-His-tag®, whole cell lysate at 50 µg
Lane 2: 293T cells transfected with a human wild-type Tau 2N4R expression vector containing a myc-His-tag® were treated with 10 uM Thiamet G for 48 hours, whole cell lysate at 50 µg
Lane 3: 293T cells transfected with a human Tau 2N4R (S400A mutation) expression vector containing a myc-His-tag®, whole cell lysate at 50 µg
Lane 4: 293T cells transfected with a human Tau 2N4R (S400A mutation) expression vector containing a myc-His-tag® were treated with 10 uM Thiamet G for 48 hours, whole cell lysate at 50 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Observed band size: 25-75 kDa, 36 kDa
Exposure time: 37s
![Immunocytochemistry/ Immunofluorescence - Anti-Tau (O-GlcNAc S400) antibody [EPR24891-48] - BSA and Azide free (ab320077), expandable thumbnail](https://content.abcam.com/products/images/tau-o-glcnac-s400-antibody-epr24891-48-bsa-and-azide-free-ab320077--immunocytochemistry-immunofluorescence-img435185.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Tau (O-GlcNAc S400) antibody [EPR24891-48] - BSA and Azide free (ab320077)")
Immunocytochemistry/ Immunofluorescence - Anti-Tau (O-GlcNAc S400) antibody [EPR24891-48] - BSA and Azide free (ab320077)
This data was developed using Anti-Tau (O-GlcNAc S400) antibody [EPR24891-48] ab320076, the same antibody clone in a different buffer formulation.
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized 293T (human embryonic kidney epithelial cell) cells labelling Tau (O-GlcNAc S400) with Anti-Tau (O-GlcNAc S400) antibody [EPR24891-48] ab320076 at 1/500 (1.02 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green).
Confocal image showing positive staining in 293T cells transfected with a human Tau 2N4R expression vector containing a myc-His-tag®, and the signal increased after treatment with Thiamet G (10 um) for 48 h. Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8).
A : 293T (human embryonic kidney epithelial cell) cells transfected with a human wild-type Tau 2N4R expression vector containing a myc-His-tag®
B : 293T cells transfected with a human wild-type Tau 2N4R expression vector containing a myc-His-tag® were treated with 10 μM Thiamet G for 48 hours
C : 293T cells transfected with a human Tau 2N4R (S400A mutation) expression vector containing a myc-His-tag®
D : 293T cells transfected with a human Tau 2N4R (S400A mutation) expression vector containing a myc-His-tag® were treated with 10 μM Thiamet G for 48 hoursAlexa Fluor® 594 Anti-Myc tag antibody [9E10] ab223894 Anti-Myc tag mouse monoclonal antibody (Alexa Fluor® 594) was used to counterstain tubulin at 1/100 5ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/ml dilution.
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