Rabbit Recombinant Monoclonal TAU antibody. Carrier free. Suitable for I-ELISA, IHC-P and reacts with Synthetic peptide - Human, Human samples.
Images
![Indirect ELISA - Anti-Tau (PDR P2 region) antibody [EPR30099-573] - BSA and Azide free (AB324253), expandable thumbnail](https://content.abcam.com/products/images/tau-pdr-p2-region-antibody-epr30099-573-bsa-and-azide-free-ab324253--indirect-elisa-img446755.jpg/_jcr_content/renditions/webp-475.webp "Indirect ELISA - Anti-Tau (PDR P2 region) antibody [EPR30099-573] - BSA and Azide free (AB324253)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (PDR P2 region) antibody [EPR30099-573] - BSA and Azide free (AB324253), expandable thumbnail](https://content.abcam.com/products/images/tau-pdr-p2-region-antibody-epr30099-573-bsa-and-azide-free-ab324253--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img446743.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (PDR P2 region) antibody [EPR30099-573] - BSA and Azide free (AB324253)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (PDR P2 region) antibody [EPR30099-573] - BSA and Azide free (AB324253), expandable thumbnail](https://content.abcam.com/products/images/tau-pdr-p2-region-antibody-epr30099-573-bsa-and-azide-free-ab324253--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img446742.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (PDR P2 region) antibody [EPR30099-573] - BSA and Azide free (AB324253)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (PDR P2 region) antibody [EPR30099-573] - BSA and Azide free (AB324253), expandable thumbnail](https://content.abcam.com/products/images/tau-pdr-p2-region-antibody-epr30099-573-bsa-and-azide-free-ab324253--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img446741.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (PDR P2 region) antibody [EPR30099-573] - BSA and Azide free (AB324253)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| I-ELISA | IHC-P | WB | |
|---|---|---|---|
| Human | Expected | Tested | Not recommended |
| Mouse | Not recommended | Not recommended | Not recommended |
| Synthetic peptide - Human | Tested | Not recommended | Not recommended |
I-ELISA
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide - Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Synthetic peptide - Human | Dilution info - | Notes - |
WB
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Synthetic peptide - Human | Dilution info - | Notes - |
Target data
Function
The protein expressed by the MAPT gene promotes microtubule assembly and stability and might be involved in establishing and maintaining neuronal polarity. Its C-terminus binds axonal microtubules, and the N-terminus binds neural plasma membrane components, suggesting that tau functions as a linker protein between the two. Axonal polarity is predetermined by MAPT localization within the neuronal cell's domain defined by the centrosome. The short isoforms allow cytoskeleton plasticity, whereas the longer isoforms may preferentially play a role in its stabilization. This supplementary information is collated from multiple sources and compiled automatically.
Alternative names
MAPTL, MTBT1, TAU, MAPT, Microtubule-associated protein tau, Neurofibrillary tangle protein, Paired helical filament-tau, PHF-tau
Rabbit Recombinant Monoclonal TAU antibody. Carrier free. Suitable for I-ELISA, IHC-P and reacts with Synthetic peptide - Human, Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR30099-573
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
Notes
ab324253 is the carrier free version of Anti-Tau (PDR P2 region) antibody [EPR30099-573] ab324252.
Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
![Indirect ELISA - Anti-Tau (PDR P2 region) antibody [EPR30099-573] - BSA and Azide free (ab324253), expandable thumbnail](https://content.abcam.com/products/images/tau-pdr-p2-region-antibody-epr30099-573-bsa-and-azide-free-ab324253--indirect-elisa-img446755.jpg "Indirect ELISA - Anti-Tau (PDR P2 region) antibody [EPR30099-573] - BSA and Azide free (ab324253)")
Indirect ELISA - Anti-Tau (PDR P2 region) antibody [EPR30099-573] - BSA and Azide free (ab324253)
This data was developed using Anti-Tau (PDR P2 region) antibody [EPR30099-573] ab324252, the same antibody clone in a different buffer formulation.
Indirect ELISA analysis of Anti-Tau (PDR P2 region) antibody [EPR30099-573] ab324252 at 1000-0 ng/ml. The Secondary antibody used was Peroxidase AffiniPure Goat Anti-Rabbit IgG, Fc fragment specific at 1:20000 dilution dilution.
Antigen: Tau (Proline-rich domain P2 region), Tau (MTBR domain).
Antigen concentration: 1000 ng/ml
This antibody is specific for the unmodified protein at aa218-230 within proline-rich domain P2
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (PDR P2 region) antibody [EPR30099-573] - BSA and Azide free (ab324253), expandable thumbnail](https://content.abcam.com/products/images/tau-pdr-p2-region-antibody-epr30099-573-bsa-and-azide-free-ab324253--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img446743.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (PDR P2 region) antibody [EPR30099-573] - BSA and Azide free (ab324253)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (PDR P2 region) antibody [EPR30099-573] - BSA and Azide free (ab324253)
Tau (PDR P2 region) Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining of Human spleen using rabbit Anti-Tau (PDR P2 region) antibody
This data was developed using Anti-Tau (PDR P2 region) antibody [EPR30099-573] ab324252, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human spleen tissue labeling Tau with Anti-Tau (PDR P2 region) antibody [EPR30099-573] ab324252 at 1/500 (1.004 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Negative control: No staining on human spleen.
The primary antibody was incubated for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrumentCounterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (PDR P2 region) antibody [EPR30099-573] - BSA and Azide free (ab324253), expandable thumbnail](https://content.abcam.com/products/images/tau-pdr-p2-region-antibody-epr30099-573-bsa-and-azide-free-ab324253--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img446742.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (PDR P2 region) antibody [EPR30099-573] - BSA and Azide free (ab324253)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (PDR P2 region) antibody [EPR30099-573] - BSA and Azide free (ab324253)
Tau (PDR P2 region) Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining of Human liver using rabbit Anti-Tau (PDR P2 region) antibody
This data was developed using Anti-Tau (PDR P2 region) antibody [EPR30099-573] ab324252, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling Tau with Anti-Tau (PDR P2 region) antibody [EPR30099-573] ab324252 at 1/500 (1.004 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Negative control: No staining on human liver.
The primary antibody was incubated for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrumentCounterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (PDR P2 region) antibody [EPR30099-573] - BSA and Azide free (ab324253), expandable thumbnail](https://content.abcam.com/products/images/tau-pdr-p2-region-antibody-epr30099-573-bsa-and-azide-free-ab324253--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img446741.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (PDR P2 region) antibody [EPR30099-573] - BSA and Azide free (ab324253)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (PDR P2 region) antibody [EPR30099-573] - BSA and Azide free (ab324253)
Tau (PDR P2 region) Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining using rabbit Anti-Tau (PDR P2 region) antibody
This data was developed using Anti-Tau (PDR P2 region) antibody [EPR30099-573] ab324252, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded (A) Human cerebrum and (B) Human Alzheimer's disease brain tissue labeling Tau with Anti-Tau (PDR P2 region) antibody [EPR30099-573] ab324252 at 1/500 (1.004 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Nearly no staining on (A) human cerebrum, positive staining on (B) human Alzheimer's disease brain.
The primary antibody was incubated for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrumentCounterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com