Anti-Tau (phospho S198) antibody [EPR2400]

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (phospho S198) antibody [EPR2400] (AB79540)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast cancer tissue sections labeling Tau with purified ab79540 at 1 : 1000 (0.423 μg/ml). Heat mediated antigen retrieval was performed using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used. PBS instead of the primary antibody was used as the negative control. Postive staining on human breast cancer without alkaline phosphatase treatment (image A). No staining on human breast cancer with alkaline phosphatase treatment (image B). The immunostaining was performed on a Leica Biosystems BOND^® RX instrument.

• IP

Lab

Immunoprecipitation - Anti-Tau (phospho S198) antibody [EPR2400] (AB79540)

Purified ab79540 at 1/50 dilution (2μg) immunoprecipitating Tau in SH-SY5Y whole cell lysate.
Lane 1 (input) : SY-SY5Y (Human neuroblastoma epithelial cell) whole cell lysate 10μg
Lane 2 (+) : ab79540 + SH-SY5Y whole cell lysate.
Lane 3 (-) : Rabbit monoclonal IgG (ab172730) instead of ab79540 in SH-SY5Y whole cell lysate.
VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/1000 dilution) was used for Western blotting.
Blocking Buffer and concentration : 5% NFDM/TBST.
Diluting buffer and concentration : 5% NFDM/TBST.
Observed band size : 50-70 kDa

All lanes:

Immunoprecipitation - Anti-Tau (phospho S198) antibody [EPR2400] (ab79540)

Predicted band size: 78 kDa

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• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (phospho S198) antibody [EPR2400] (AB79540)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat cerebrum tissue sections labeling Tau with purified ab79540 at 1 : 1000 (0.423 μg/ml). Heat mediated antigen retrieval was performed using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used. PBS instead of the primary antibody was used as the negative control. Postive staining on rat cerebrum without alkaline phosphatase treatment (image A). No staining on rat cerebrum with alkaline phosphatase treatment (image B). The immunostaining was performed on a Leica Biosystems BOND^® RX instrument.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (phospho S198) antibody [EPR2400] (AB79540)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse cerebrum tissue sections labeling Tau with purified ab79540 at 1 : 1000 (0.423 μg/ml). Heat mediated antigen retrieval was performed using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used. PBS instead of the primary antibody was used as the negative control. Postive staining on mouse cerebrum without alkaline phosphatase treatment (image A). No staining on mouse cerebrum with alkaline phosphatase treatment (image B). The immunostaining was performed on a Leica Biosystems BOND^® RX instrument.

• WB

Supplier Data

Western blot - Anti-Tau (phospho S198) antibody [EPR2400] (AB79540)

All lanes:

Western blot - Anti-Tau (phospho S198) antibody [EPR2400] (ab79540) at 1/1000 dilution

Lane 1:

Untreated SH-SY5Y (Human neuroblastoma epithelial cell) whole cell lysate at 15 µg

Lane 2:

SH-SY5Y treated with 1µM okadaic acid and 200nM calyculin a for 60 minutes, whole cell lysate at 15 µg

Lane 3:

SH-SY5Y treated with 1µM okadaic acid and 200nM calyculin a for 60 minutes whole cell lysate, then the membrane treated with Alkaline Phosphatase for 1 hour at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 78 kDa

Observed band size: 50-70 kDa

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• WB

Supplier Data

Western blot - Anti-Tau (phospho S198) antibody [EPR2400] (AB79540)

All lanes:

Western blot - Anti-Tau (phospho S198) antibody [EPR2400] (ab79540) at 1/10000 dilution

Lane 1:

Mouse hippocampus lysate at 15 µg

Lane 2:

Mouse hippocampus lysate, the membrane treated with Alkaline Phosphatase for 1 hour at 15 µg

Lane 3:

Rat hippocampus lysate at 15 µg

Lane 4:

Rat hippocampus lysate, the membrane treated with Alkaline Phosphatase for 1 hour at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 78 kDa

Observed band size: 50-70 kDa

false

• WB

CiteAb

Western blot - Anti-Tau (phospho S198) antibody [EPR2400] (AB79540)

Western Blotting using Anti-Tau (phospho S198) antibody [EPR2400], ab79540. Publication image from Ercan, E. et al., 2017, Mol Neurodegener, 29157277. Legend direct from paper.

A number of antibodies failed to detect tau in hTg-Tau brain lysates. a Immunoblotting with recombinant tau (full length (1–441), truncated (1–421) and caspase 3 cleaved ((1–441) + Casp3) shows that all tau forms can be detected with a pan-tau antibody (Dako, left), whereas the C3-D421 antibody only detects the truncated and the caspase 3 cleaved form of tau (right). b and c Tau isoform-specific antibodies failed to detect tau in hTg-Tau brain lysates as well as the recombinant protein in the tau ladder. d, e, f and g No signal was detected by the meK311, pT212, pS238 and C3-D421 antibodies in hTg-Tau mouse brains, possibly due to the low abundance of these modifications. h Actin demonstrates equal loading for all samples. Tg : hTg-Tau mouse, KO : Tau-KO mouse brain lysate, TL : recombinant tau ladder

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